The objective: to evaluate the possibility of determining the levels of activity of N-acetyl-β-D-... more The objective: to evaluate the possibility of determining the levels of activity of N-acetyl-β-D-glucosaminidase B (NAG B) in urine as an enzymological marker of differentiation of ischemic damage to the renal parenchyma in children with congenital vesicoureteral reflux (VUR). Materials and methods. 49 children aged 1 to 15 years with a verified diagnosis of unilateral VUR and 25 practically healthy children were examined. NAG B activity in urine was determined before reconstructive treatment. The limits of the reference intervals of the levels of enzyme activity in the urine of healthy children were calculated by the formula M±1.5σ. According to the personalized analysis the patientswere divided into 3 groups: group 1 – 13 patients (the levels of NAG activity in the urine are registered from 0.8 to 2.3 units); group 2 – 14 patients (the levels of NAG activity in the urine – 2.4 to 4.5 units); group 3 – 22 patients (the levels of NAG activity in urine – 4.6 to 10.5 units. Results. I...
Congenital anomalies of the uretero-vesical junction (CAUVJ) are frequent cause of chronic kidney... more Congenital anomalies of the uretero-vesical junction (CAUVJ) are frequent cause of chronic kidney disease in children and if not timely corrected can lead to nephrosclerosis, hypertension and chronic renal failure. The modern arsenal of methods UVJ reconstruction includes transvesical and extravesical ureterocystoneostomy. With the transvesical approach due to trauma, the functional state of the detrusor can significantly deteriorate for a long time (up to 3 weeks), which further compromises the functions of the upper urinary tract and kidneys. In these circumstances, the scientific justification of the safest method of correction of CAUVJ is an actual problem. The advantages of determining the complex of biomarkers in the urine to assess the functional state of the kidney include high informativeness and non-invasiveness. The objective: to determine the optimal method of surgical correction of CAUVJ in children by assessing the dynamics of changes in the complex of marker cytokines...
Immunology and Allergy: Science and Practice, 2020
The aim of this study was to study the peculiarities of changes in cytokine excretion levels of t... more The aim of this study was to study the peculiarities of changes in cytokine excretion levels of transforming growth factor b1 (TGF-b1), tumor necrosis factor a (TNF-a), monocytic chemoattractant protein 1 (MCP-1) and urinary activity of conditionally renospecific enzymes g-glutamyltranspeptidase (GGT) and neutral a-gtlucosidase (NG) as biomarkers of restoration of the functional state of the kidney parenchyma in children at different times after reconstructive correction of congenital defects of the uretero-vesical segment (UVS). Materials and Methods. Prior to reconstructive correction of congenital defects of UVS the comprehensive clinical and laboratory study was conducted in 45 children aged 4 to 15 years (22 boys, 23 girls) who were diagnosed with megaureter (MU) according to visualization methods: 18 patients with non-refluxing MU (NRMU) and 27 patients with refluxing MU (RMU). 3-4 patients were examined 3-4 weeks after the operation and 24 patients 4-6 months later. The study...
The objective: to evaluate the possibility of determining the levels of activity of N-acetyl-β-D-... more The objective: to evaluate the possibility of determining the levels of activity of N-acetyl-β-D-glucosaminidase B (NAG B) in urine as an enzymological marker of differentiation of ischemic damage to the renal parenchyma in children with congenital vesicoureteral reflux (VUR). Materials and methods. 49 children aged 1 to 15 years with a verified diagnosis of unilateral VUR and 25 practically healthy children were examined. NAG B activity in urine was determined before reconstructive treatment. The limits of the reference intervals of the levels of enzyme activity in the urine of healthy children were calculated by the formula M±1.5σ. According to the personalized analysis the patientswere divided into 3 groups: group 1 – 13 patients (the levels of NAG activity in the urine are registered from 0.8 to 2.3 units); group 2 – 14 patients (the levels of NAG activity in the urine – 2.4 to 4.5 units); group 3 – 22 patients (the levels of NAG activity in urine – 4.6 to 10.5 units. Results. I...
Congenital anomalies of the uretero-vesical junction (CAUVJ) are frequent cause of chronic kidney... more Congenital anomalies of the uretero-vesical junction (CAUVJ) are frequent cause of chronic kidney disease in children and if not timely corrected can lead to nephrosclerosis, hypertension and chronic renal failure. The modern arsenal of methods UVJ reconstruction includes transvesical and extravesical ureterocystoneostomy. With the transvesical approach due to trauma, the functional state of the detrusor can significantly deteriorate for a long time (up to 3 weeks), which further compromises the functions of the upper urinary tract and kidneys. In these circumstances, the scientific justification of the safest method of correction of CAUVJ is an actual problem. The advantages of determining the complex of biomarkers in the urine to assess the functional state of the kidney include high informativeness and non-invasiveness. The objective: to determine the optimal method of surgical correction of CAUVJ in children by assessing the dynamics of changes in the complex of marker cytokines...
Immunology and Allergy: Science and Practice, 2020
The aim of this study was to study the peculiarities of changes in cytokine excretion levels of t... more The aim of this study was to study the peculiarities of changes in cytokine excretion levels of transforming growth factor b1 (TGF-b1), tumor necrosis factor a (TNF-a), monocytic chemoattractant protein 1 (MCP-1) and urinary activity of conditionally renospecific enzymes g-glutamyltranspeptidase (GGT) and neutral a-gtlucosidase (NG) as biomarkers of restoration of the functional state of the kidney parenchyma in children at different times after reconstructive correction of congenital defects of the uretero-vesical segment (UVS). Materials and Methods. Prior to reconstructive correction of congenital defects of UVS the comprehensive clinical and laboratory study was conducted in 45 children aged 4 to 15 years (22 boys, 23 girls) who were diagnosed with megaureter (MU) according to visualization methods: 18 patients with non-refluxing MU (NRMU) and 27 patients with refluxing MU (RMU). 3-4 patients were examined 3-4 weeks after the operation and 24 patients 4-6 months later. The study...
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