Asian Journal of Pharmaceutical and Clinical Research, 2016
The metal nanoparticle synthesis is highly explored the field of nanotechnology. The biological m... more The metal nanoparticle synthesis is highly explored the field of nanotechnology. The biological methods seem to be more effective because of slow reduction rate and polydispersity of the final products. The main aim of this study is too the rapid and simplistic synthesis of silver nanoparticles by Withania somnifera Linn. at room temperature. The exposure of reaction mixtures containing silver nitrate and dried leaf powder of W. somnifera resulted in reduction of metal ions within 5 minutes. The extracellular synthesized silver nanoparticles were characterized by ultraviolet-visible, infrared (IR) spectroscopy, X-ray diffraction studies, zeta potential, Fourier transform IR, and scanning electron microscopy. The antibacterial and antifungal studies showed significant activity as compared to their respective standards. From the results, W. somnifera sliver nanoparticle has attained the maximum antimicrobial against clinical pathogens and also seen very good stability of nanoparticle ...
Polish journal of microbiology / Polskie Towarzystwo Mikrobiologów = The Polish Society of Microbiologists
The present experiments were studied on bioremediation of denim industry wastewater by using poly... more The present experiments were studied on bioremediation of denim industry wastewater by using polyurethane foam (PU foam) immobilized bacterial cells. About 30 indigenous adapted bacterial strains were isolated from denim textile effluent out of which only four isolates were found to be efficient against crude indigo carmine degradation using broth decolorization method. The selected bacterial strains were identified as Actinomyces sp., (PK 07), Pseudomonas sp., (PK 18), Stenotrophomonas sp., (PK 23) and Staphylococcus sp., (PK 28) based on microscopic and biochemical characteristics. The bacterial immobilized cells have the highest number of viable cells (PK07, PK18, PK23 and PK28 appeared to be 1×108, 1×109, 1×106 and 1×107 CFU/ml respectively) and maximum attachment efficiency of 92% on PU foam. The complete degradation using a consortium of PU foam immobilized cells was achieved at pH 6, 27°C, 100% of substrate concentration and allowed to develop biofilm for one day (1.5% W/V). ...
Bioremediation of Tannery Wastewater Using Immobilized Marine Microalga Tetraselmis sp.: Experime... more Bioremediation of Tannery Wastewater Using Immobilized Marine Microalga Tetraselmis sp.: Experimental Studies and Pseudo-Second Order Kinetics The removals of pollutants (nitrate, silicate, chromium and sulphide) from tannery wastewater were studied in batch experiments using marine microalgae. In this study five different microalgae species were used, out of these Tetraselmis sp. was found to be the most promising one. This species was further studied by employing several factors like variation in pH, retention time, algal cell density and beads density in order to understand the effect of biosorption on the pollutants. The higher biosorption percentage was achieved with a short retention time, while the sorption capacity of the algal cell density increased with the rise in the concentration of pollutants.
_____________________________________________________________________________________________ ABS... more _____________________________________________________________________________________________ ABSTRACT The soil samples were collected from oil factory in Salem, Vellore and Chennai. About 12 organisms were isolated from these soil samples. The strain was then screened using MM9 media for the L-asparaginase enzyme production. Aspergillus niger which showed pink colour around the colony on selected media. The screened isolate was optimized at different substrates (wheat bran, gingelly oil cake, cotton seed, rice bran, rice bran), temperature (30°C, 40°C, 50°C, 60°C and 70°C), moisture content (10%, 20%, 30%, 40% and 50%), pH (5, 6, 7, 8 and 9), incubation days (3, 4, 5, 6 and 7), carbon sources (glucose, maltose, sucrose, lactose and mannitol), nitrogen sources (urea, peptone, casein and yeast extract) and metal ions (ZnCl 2 , FeSO 4 , MgSO 4 , NaCl, and CaCl 2). A.niger produced maximum L-asparaginase enzyme in gingelly oil cake at temperature 40°C, in 30 % of moisture content, at p...
Aims: To study the microbial degradation of hydrocarbon from oil contaminated soil sample using P... more Aims: To study the microbial degradation of hydrocarbon from oil contaminated soil sample using Pseudomonas sp. and were analyzed the different physicochemical characters of normal and oil contaminated soil. Method and Results: The soil sample was collected from oil contaminated site, in Sivakasi and then the oil degrading microorganism (Pseudomonas sp) was screened from oil contaminated soil by using enrichment technique. After, the isolated microorganisms were identified by using standard characterization method. From the oil contaminated soil, 10 bacterial strains were isolated. Among them, tolerance of two strains was used for further studies. The physico-chemical characters of normal and oil contaminated soil samples were analyzed by standard method. In this study, the pH, moisture, carbon, phosphorous and potassium content of normal and oil contaminated soil samples were analyzed and compared. In the present investigation, the various concentration of aromatic hydrocarbon (tol...
This paper presents the review of the effects of bioremediation on the denim textile effluent usi... more This paper presents the review of the effects of bioremediation on the denim textile effluent using adapted bacterial cells immobilized on polyurethane foam. It further examines the various methods used in the biodecolorization of the industrial wastewater including the action of oxidative enzymes. Bacterial biofilms have great potential for the sustainable production of enzymes because of their high resistant, inherent characteristic of self immobilization and long term activity. It was also found that bacterial biofilm is widely used to treat textile wastewater because of the advantage derived from its ability to withstand under extreme conditions with their action of oxidoreductase enzymes
Abstract: Indole Acetic Acid (IAA) producing bacterium was isolated from the Rhizosphere soil and... more Abstract: Indole Acetic Acid (IAA) producing bacterium was isolated from the Rhizosphere soil and identified as Rhizobium sp. and Bacillus sp., Optimization of Indole acetic acid production was carried out at different cultural conditions, such as pH, temperature and ...
Abstract: This study investigated the potential of adapted fungi isolated from textile effluent c... more Abstract: This study investigated the potential of adapted fungi isolated from textile effluent containing a mixture of azo which were used in the bioremediation analysis. Around 4 organisms with a potential for color removal was selected and were found to be ...
Address:Name:Prabhavathi. PPlace:Tamil Nadu, IndiaE-mail:prabhamicro.19@gmail.comABSTRACTBiosurfa... more Address:Name:Prabhavathi. PPlace:Tamil Nadu, IndiaE-mail:prabhamicro.19@gmail.comABSTRACTBiosurfactants are valuable microbial amphiphilic molecules with effective surface activeand biological properties applicable to several industries and their processes. Microbessynthesize them, especially during growth on water immiscible substrates, providing analternative chemically prepared conventional surfactants. In recent years naturalbiosurfactants have attracted attention because of their low toxicity, biodegradability andecological acceptability. These molecules could be widely used in cosmetic,pharmaceutical and food processes as emulsifiers, preservatives and detergents and inbioremediation processes. The present study was aimed to screen and to evaluate thebiosurfactant and heavy metal resistant activity of biosurfactant produced by L. lactis.Lactococcus lactis was isolated from homemade curd was found to be a potent producer oflipopeptide biosurfactant with olive oil as carbon source. In continuation of these studies,the present work intended to purify the biosurfactant produced by the indigenousprobiotic bacteria L. lactis, structural analysis of the biosurfactant and to apply thepurified biosurfactant in microbial decolonization, bacteriocidal, heavy metalbioremediation and crude oil degradation.Keywords: biosurfactant, metal resistant bacteria, TLC.
"The synthesis of nanoparticles from plant sources has proved to be an effec... more "The synthesis of nanoparticles from plant sources has proved to be an effective and alternate method for the novel production of nanoparticles. A number of synthesis techniques have been developed including the chemical reduction of silver ions in aqueous solutions, with or without stabilizing agents, thermal decomposition in inorganic solvent, and chemical and photo reduction. Our study deals with the synthesis of silver nanoparticles from Cardiospermum helicacabum leaf extracts mostly fresh. The samples consisted of the leaf extracts at two different temperatures 65°C (5min,10min,15 min) and 95°C for 5 min. The plant extract at 95°C, however showed better and accurate nanoparticles formation with AgNO3 with time. Thus showing that the extraction temperature plays an important role in formation of nanoparticles. Also U.V Spectrophotometer studies show a graph that shows gradual increase of nanoparticles concentration with time, temperature, and sample characteristics. From these results we can conclude that Cardiospermum helicacabum is a potential source for nanoparticles synthesis and have identified an optimum temperature for its efficient synthesis. Depending Upon the particle size application of the nanoparticles varies. Particle size, shape can be determined by characterization procedure SEM and XRD .Synthesizing Silver nanoparticles by medicinal plants is echo friendly and good antimicrobial efficiency against bacteria, viruses and other micro-organisms. Now days widely applicable in medicinal fields for Cancer treatments, AIDS, Epilepsy etc. Keywords: Silver nanoparticles, Cardiospermum helicacabum, AgNO3"
Asian Journal of Pharmaceutical and Clinical Research, 2016
The metal nanoparticle synthesis is highly explored the field of nanotechnology. The biological m... more The metal nanoparticle synthesis is highly explored the field of nanotechnology. The biological methods seem to be more effective because of slow reduction rate and polydispersity of the final products. The main aim of this study is too the rapid and simplistic synthesis of silver nanoparticles by Withania somnifera Linn. at room temperature. The exposure of reaction mixtures containing silver nitrate and dried leaf powder of W. somnifera resulted in reduction of metal ions within 5 minutes. The extracellular synthesized silver nanoparticles were characterized by ultraviolet-visible, infrared (IR) spectroscopy, X-ray diffraction studies, zeta potential, Fourier transform IR, and scanning electron microscopy. The antibacterial and antifungal studies showed significant activity as compared to their respective standards. From the results, W. somnifera sliver nanoparticle has attained the maximum antimicrobial against clinical pathogens and also seen very good stability of nanoparticle ...
Polish journal of microbiology / Polskie Towarzystwo Mikrobiologów = The Polish Society of Microbiologists
The present experiments were studied on bioremediation of denim industry wastewater by using poly... more The present experiments were studied on bioremediation of denim industry wastewater by using polyurethane foam (PU foam) immobilized bacterial cells. About 30 indigenous adapted bacterial strains were isolated from denim textile effluent out of which only four isolates were found to be efficient against crude indigo carmine degradation using broth decolorization method. The selected bacterial strains were identified as Actinomyces sp., (PK 07), Pseudomonas sp., (PK 18), Stenotrophomonas sp., (PK 23) and Staphylococcus sp., (PK 28) based on microscopic and biochemical characteristics. The bacterial immobilized cells have the highest number of viable cells (PK07, PK18, PK23 and PK28 appeared to be 1×108, 1×109, 1×106 and 1×107 CFU/ml respectively) and maximum attachment efficiency of 92% on PU foam. The complete degradation using a consortium of PU foam immobilized cells was achieved at pH 6, 27°C, 100% of substrate concentration and allowed to develop biofilm for one day (1.5% W/V). ...
Bioremediation of Tannery Wastewater Using Immobilized Marine Microalga Tetraselmis sp.: Experime... more Bioremediation of Tannery Wastewater Using Immobilized Marine Microalga Tetraselmis sp.: Experimental Studies and Pseudo-Second Order Kinetics The removals of pollutants (nitrate, silicate, chromium and sulphide) from tannery wastewater were studied in batch experiments using marine microalgae. In this study five different microalgae species were used, out of these Tetraselmis sp. was found to be the most promising one. This species was further studied by employing several factors like variation in pH, retention time, algal cell density and beads density in order to understand the effect of biosorption on the pollutants. The higher biosorption percentage was achieved with a short retention time, while the sorption capacity of the algal cell density increased with the rise in the concentration of pollutants.
_____________________________________________________________________________________________ ABS... more _____________________________________________________________________________________________ ABSTRACT The soil samples were collected from oil factory in Salem, Vellore and Chennai. About 12 organisms were isolated from these soil samples. The strain was then screened using MM9 media for the L-asparaginase enzyme production. Aspergillus niger which showed pink colour around the colony on selected media. The screened isolate was optimized at different substrates (wheat bran, gingelly oil cake, cotton seed, rice bran, rice bran), temperature (30°C, 40°C, 50°C, 60°C and 70°C), moisture content (10%, 20%, 30%, 40% and 50%), pH (5, 6, 7, 8 and 9), incubation days (3, 4, 5, 6 and 7), carbon sources (glucose, maltose, sucrose, lactose and mannitol), nitrogen sources (urea, peptone, casein and yeast extract) and metal ions (ZnCl 2 , FeSO 4 , MgSO 4 , NaCl, and CaCl 2). A.niger produced maximum L-asparaginase enzyme in gingelly oil cake at temperature 40°C, in 30 % of moisture content, at p...
Aims: To study the microbial degradation of hydrocarbon from oil contaminated soil sample using P... more Aims: To study the microbial degradation of hydrocarbon from oil contaminated soil sample using Pseudomonas sp. and were analyzed the different physicochemical characters of normal and oil contaminated soil. Method and Results: The soil sample was collected from oil contaminated site, in Sivakasi and then the oil degrading microorganism (Pseudomonas sp) was screened from oil contaminated soil by using enrichment technique. After, the isolated microorganisms were identified by using standard characterization method. From the oil contaminated soil, 10 bacterial strains were isolated. Among them, tolerance of two strains was used for further studies. The physico-chemical characters of normal and oil contaminated soil samples were analyzed by standard method. In this study, the pH, moisture, carbon, phosphorous and potassium content of normal and oil contaminated soil samples were analyzed and compared. In the present investigation, the various concentration of aromatic hydrocarbon (tol...
This paper presents the review of the effects of bioremediation on the denim textile effluent usi... more This paper presents the review of the effects of bioremediation on the denim textile effluent using adapted bacterial cells immobilized on polyurethane foam. It further examines the various methods used in the biodecolorization of the industrial wastewater including the action of oxidative enzymes. Bacterial biofilms have great potential for the sustainable production of enzymes because of their high resistant, inherent characteristic of self immobilization and long term activity. It was also found that bacterial biofilm is widely used to treat textile wastewater because of the advantage derived from its ability to withstand under extreme conditions with their action of oxidoreductase enzymes
Abstract: Indole Acetic Acid (IAA) producing bacterium was isolated from the Rhizosphere soil and... more Abstract: Indole Acetic Acid (IAA) producing bacterium was isolated from the Rhizosphere soil and identified as Rhizobium sp. and Bacillus sp., Optimization of Indole acetic acid production was carried out at different cultural conditions, such as pH, temperature and ...
Abstract: This study investigated the potential of adapted fungi isolated from textile effluent c... more Abstract: This study investigated the potential of adapted fungi isolated from textile effluent containing a mixture of azo which were used in the bioremediation analysis. Around 4 organisms with a potential for color removal was selected and were found to be ...
Address:Name:Prabhavathi. PPlace:Tamil Nadu, IndiaE-mail:prabhamicro.19@gmail.comABSTRACTBiosurfa... more Address:Name:Prabhavathi. PPlace:Tamil Nadu, IndiaE-mail:prabhamicro.19@gmail.comABSTRACTBiosurfactants are valuable microbial amphiphilic molecules with effective surface activeand biological properties applicable to several industries and their processes. Microbessynthesize them, especially during growth on water immiscible substrates, providing analternative chemically prepared conventional surfactants. In recent years naturalbiosurfactants have attracted attention because of their low toxicity, biodegradability andecological acceptability. These molecules could be widely used in cosmetic,pharmaceutical and food processes as emulsifiers, preservatives and detergents and inbioremediation processes. The present study was aimed to screen and to evaluate thebiosurfactant and heavy metal resistant activity of biosurfactant produced by L. lactis.Lactococcus lactis was isolated from homemade curd was found to be a potent producer oflipopeptide biosurfactant with olive oil as carbon source. In continuation of these studies,the present work intended to purify the biosurfactant produced by the indigenousprobiotic bacteria L. lactis, structural analysis of the biosurfactant and to apply thepurified biosurfactant in microbial decolonization, bacteriocidal, heavy metalbioremediation and crude oil degradation.Keywords: biosurfactant, metal resistant bacteria, TLC.
"The synthesis of nanoparticles from plant sources has proved to be an effec... more "The synthesis of nanoparticles from plant sources has proved to be an effective and alternate method for the novel production of nanoparticles. A number of synthesis techniques have been developed including the chemical reduction of silver ions in aqueous solutions, with or without stabilizing agents, thermal decomposition in inorganic solvent, and chemical and photo reduction. Our study deals with the synthesis of silver nanoparticles from Cardiospermum helicacabum leaf extracts mostly fresh. The samples consisted of the leaf extracts at two different temperatures 65°C (5min,10min,15 min) and 95°C for 5 min. The plant extract at 95°C, however showed better and accurate nanoparticles formation with AgNO3 with time. Thus showing that the extraction temperature plays an important role in formation of nanoparticles. Also U.V Spectrophotometer studies show a graph that shows gradual increase of nanoparticles concentration with time, temperature, and sample characteristics. From these results we can conclude that Cardiospermum helicacabum is a potential source for nanoparticles synthesis and have identified an optimum temperature for its efficient synthesis. Depending Upon the particle size application of the nanoparticles varies. Particle size, shape can be determined by characterization procedure SEM and XRD .Synthesizing Silver nanoparticles by medicinal plants is echo friendly and good antimicrobial efficiency against bacteria, viruses and other micro-organisms. Now days widely applicable in medicinal fields for Cancer treatments, AIDS, Epilepsy etc. Keywords: Silver nanoparticles, Cardiospermum helicacabum, AgNO3"
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