Introduction: Water deficit is one of the major limiting factors that influences the cultivation ... more Introduction: Water deficit is one of the major limiting factors that influences the cultivation and production of agricultural crops in the world. Drought stress severely impairs plants growth and development through alterations in the physiological, morphological, biochemical, and molecular attributes (Cheng et al., 2018). Most of the world's farms are located in arid and semi-arid regions. In addition, drought conditions can be exacerbated by climate change and water scarcity. Therefore, it is important to find a way for increasing the adaptation potential of crops to water shortage (Asghari et al., 2020). Zeolite can act as water moderators and play a key role in plant growth and development under drought condition. The aim of this research was to investigate the effect of zeolite application on morphological traits and secondary metabolite biosynthesis of dill (Anethum graveolens L.) under drought stress. Material and methods: This study was conducted as a factorial experim...
For the study of salinity stress on <em>Vicia villosa</em> and calcium effect for mod... more For the study of salinity stress on <em>Vicia villosa</em> and calcium effect for modulation of that, an experiment was conducted under hydroponic condition, and some important morphological and physiological characteristics were evaluated. This experiment was conducted as a factorial based on randomized complete design with three replications. The treatments include salinity stress in three levels (0, 50, and 100 mM NaCl) and calcium in two levels (content in Hoagland solution and double content). The results showed that all morphological and physiological traits include root and shoot length, root and shoot wet and dry weight, leaf area, leaf chlorophyll content, RWC, CMS, and biological yield was significantly different from the control and is affected by the salinity stress severely. But, calcium effect on them was not significant despite of decreasing salinity effect.
Journal of Pharmaceutical Research International, 2019
Aims: Helicobacter pylori infection spreads widely in the world and has several complications inc... more Aims: Helicobacter pylori infection spreads widely in the world and has several complications including gastric cancer. The aim of this work is to investigate the antimicrobial properties of Valeriana officinalis L., Satureja bachtiarica and Thymus daenensis extracts against H. pylori. Methods: The disk diffusion test was used in this experiment. Methanolic extract was isolated from Valeriana officinalis L., Satureja bachtiarica and Thymus daenensis. Ten clinical isolates of H. pylori were used in this experiment, which was obtained at Alzahra hospital, Tehran, Iran from individual patients with gastrointestinal disorders. H. pylori strains were identified using the checking of bacterial growth (0.5 – 1 mm), Gram staining, urease test, catalase test and the drug resistance standard was performed on them. To determine the MIC of the extracts, disk diffusion (Kirby-Bauer) test in agar was used. Results: The clinical strains showed the highest susceptibility to tetracycline (10%). The ...
Abstract Salinity is serious environmental stress that highly affects the quantity and quality of... more Abstract Salinity is serious environmental stress that highly affects the quantity and quality of herbal products. In this study a pot experiment was carried out in greenhouse to investigate the interactive effects of Azospirillum lipoferum and Piriformospora indica on growth attributes and secondary metabolites production of sesame (Sesamum indicum L.) under saline condition. The applied microorganisms raised the ability of sesame in absorption of essential nutrients. The highest concentration of N (24.33 and 39.65 mg g−1 DW in shoot and root, respectively), K (23.13 and 18.74 mg g−1 DW in shoot and root, respectively) and P (11.08 and 13.70 mg g−1 DW in shoot and root, respectively) were observed in co-inoculated plants under non-stress condition. The results showed that increasing NaCl concentration in irrigation water (from 0 to 120 mM), significantly disordered the absorption and distribution of water and minerals. In addition, salt-induced reactive oxygen species lead to oxidative damage of sesame plants, consequently, 3.29 and 1.87 fold increases were observed in the amount of malondialdehyde (MDA) and electrolyte leakage (EL), respectively. During salt stress, activity of protective enzymes and amount of non-enzymatic antioxidants were dramatically raised. This study showed that inoculation of sesame with PBRMs can provide salt-tolerance by changing in physio-biochemical responses. Under saline condition, inoculation with Al and Pi resulted an enhancement of relative water content (up to 20%), maximum photochemical quantum yield of PSII (Fv/Fm) (up to 25%), antioxidant enzyme activity, nutrient absorption, proline (36–65%) and secondary metabolite contents, DPPH radical scavenging activity (18–22%) and linoleic acid. Conversely, the amount of MDA (up to 35%), EL (up to 23%), Na+ (up to 66 and 59% in shoot and root, respectively) as well as oleic/linoleic acids ratio were significantly decreased via microbial treatments. However, combined inoculation (Al + Pi) was generally more effective in alleviation of salinity deleterious effects than individual inoculation.
12 2 Abstract: Crop plants are usually affected by biotic and abiotic stresses that reduce their ... more 12 2 Abstract: Crop plants are usually affected by biotic and abiotic stresses that reduce their relative yields. Soil salinity is abiotic stress factor that poses a serious threat to agricultural production, as more than 800 million hectares of land in the world have been salt affected, which account for 6% of the total land area. Impacts of climatic and anthropogenic factors such as soil salinity, soil acidity and depletion of soil organic matter leading to reduced soil productivity continue to be a constraint and will become more serious under climate change and variability. Understanding the molecular basis of salinity will be helpful in developing selection strategies for improving salinity tolerance. To determine gene function relationships, large scale genome-wide reverse genetics approaches have been developed which includes both non-transgenic technology platforms such as TILLING (targeting induced local lesions in genomes) and EcoTILLING and insertional mutagenesis systems...
During postharvest life, broccoli suffers from floret yellowing confining its economic and nutrit... more During postharvest life, broccoli suffers from floret yellowing confining its economic and nutritional value. The objective of the present study was to explore the mechanisms employed by phytosulfokine α (PSKα) at 150 nM for delaying floret yellowing in broccoli during storage at 4°C for 28 days. Our results showed that the higher endogenous accumulation of hydrogen sulfide (H2S) resulting from the higher gene expression and activities of l-cysteine desulfhydrase (LCD) and d-cysteine desulfhydrase (DCD) in broccoli floret treated with 150 nM PSKα may serve as an endogenous signaling molecule for delaying senescence. Moreover, the suppressed ethylene biosynthesis in broccoli floret treated with 150 nM PSKα might be ascribed to lower gene expression and activities of ACC synthase (ACS) and ACC oxidase (ACO). Furthermore, lower gene expression and activities of Mg2+ dechelatase (MDC), pheophytinase (PPH), and pheophorbide a oxygenase (PaO) might be the reasons for the higher accumulati...
Wheat is one of the most important cereals, which is very valuable in food. Haploid plants are of... more Wheat is one of the most important cereals, which is very valuable in food. Haploid plants are of particular importance in plant breeding. The wheat seeds produced in the crosses between wheat and maize in the chromosome elimination method without of endosperm and are immature embryo, to prevent the abortions haploid embryos, they must have embryo rescue. Increasing production of haploid plants from produced embryos can improve production efficiency. In this study, With attention their effects gibberellic acid and 2,4-Dichlorophenoxyacetic acid on growth, cell size and cell division, Their use in the production of wheat haploid plant were studied. There was a significant difference at level 1% between the not use and use of gibberellic acid in difference times in the production of haploid from embryos, So that the most haploid plant produced in the use of gibberellic acid in the 4 days after pollination. Also, the use of 2,4-Dichlorophenoxyacetic acid in tiller maintenance liquid cu...
Introduction: Water deficit is one of the major limiting factors that influences the cultivation ... more Introduction: Water deficit is one of the major limiting factors that influences the cultivation and production of agricultural crops in the world. Drought stress severely impairs plants growth and development through alterations in the physiological, morphological, biochemical, and molecular attributes (Cheng et al., 2018). Most of the world's farms are located in arid and semi-arid regions. In addition, drought conditions can be exacerbated by climate change and water scarcity. Therefore, it is important to find a way for increasing the adaptation potential of crops to water shortage (Asghari et al., 2020). Zeolite can act as water moderators and play a key role in plant growth and development under drought condition. The aim of this research was to investigate the effect of zeolite application on morphological traits and secondary metabolite biosynthesis of dill (Anethum graveolens L.) under drought stress. Material and methods: This study was conducted as a factorial experim...
For the study of salinity stress on <em>Vicia villosa</em> and calcium effect for mod... more For the study of salinity stress on <em>Vicia villosa</em> and calcium effect for modulation of that, an experiment was conducted under hydroponic condition, and some important morphological and physiological characteristics were evaluated. This experiment was conducted as a factorial based on randomized complete design with three replications. The treatments include salinity stress in three levels (0, 50, and 100 mM NaCl) and calcium in two levels (content in Hoagland solution and double content). The results showed that all morphological and physiological traits include root and shoot length, root and shoot wet and dry weight, leaf area, leaf chlorophyll content, RWC, CMS, and biological yield was significantly different from the control and is affected by the salinity stress severely. But, calcium effect on them was not significant despite of decreasing salinity effect.
Journal of Pharmaceutical Research International, 2019
Aims: Helicobacter pylori infection spreads widely in the world and has several complications inc... more Aims: Helicobacter pylori infection spreads widely in the world and has several complications including gastric cancer. The aim of this work is to investigate the antimicrobial properties of Valeriana officinalis L., Satureja bachtiarica and Thymus daenensis extracts against H. pylori. Methods: The disk diffusion test was used in this experiment. Methanolic extract was isolated from Valeriana officinalis L., Satureja bachtiarica and Thymus daenensis. Ten clinical isolates of H. pylori were used in this experiment, which was obtained at Alzahra hospital, Tehran, Iran from individual patients with gastrointestinal disorders. H. pylori strains were identified using the checking of bacterial growth (0.5 – 1 mm), Gram staining, urease test, catalase test and the drug resistance standard was performed on them. To determine the MIC of the extracts, disk diffusion (Kirby-Bauer) test in agar was used. Results: The clinical strains showed the highest susceptibility to tetracycline (10%). The ...
Abstract Salinity is serious environmental stress that highly affects the quantity and quality of... more Abstract Salinity is serious environmental stress that highly affects the quantity and quality of herbal products. In this study a pot experiment was carried out in greenhouse to investigate the interactive effects of Azospirillum lipoferum and Piriformospora indica on growth attributes and secondary metabolites production of sesame (Sesamum indicum L.) under saline condition. The applied microorganisms raised the ability of sesame in absorption of essential nutrients. The highest concentration of N (24.33 and 39.65 mg g−1 DW in shoot and root, respectively), K (23.13 and 18.74 mg g−1 DW in shoot and root, respectively) and P (11.08 and 13.70 mg g−1 DW in shoot and root, respectively) were observed in co-inoculated plants under non-stress condition. The results showed that increasing NaCl concentration in irrigation water (from 0 to 120 mM), significantly disordered the absorption and distribution of water and minerals. In addition, salt-induced reactive oxygen species lead to oxidative damage of sesame plants, consequently, 3.29 and 1.87 fold increases were observed in the amount of malondialdehyde (MDA) and electrolyte leakage (EL), respectively. During salt stress, activity of protective enzymes and amount of non-enzymatic antioxidants were dramatically raised. This study showed that inoculation of sesame with PBRMs can provide salt-tolerance by changing in physio-biochemical responses. Under saline condition, inoculation with Al and Pi resulted an enhancement of relative water content (up to 20%), maximum photochemical quantum yield of PSII (Fv/Fm) (up to 25%), antioxidant enzyme activity, nutrient absorption, proline (36–65%) and secondary metabolite contents, DPPH radical scavenging activity (18–22%) and linoleic acid. Conversely, the amount of MDA (up to 35%), EL (up to 23%), Na+ (up to 66 and 59% in shoot and root, respectively) as well as oleic/linoleic acids ratio were significantly decreased via microbial treatments. However, combined inoculation (Al + Pi) was generally more effective in alleviation of salinity deleterious effects than individual inoculation.
12 2 Abstract: Crop plants are usually affected by biotic and abiotic stresses that reduce their ... more 12 2 Abstract: Crop plants are usually affected by biotic and abiotic stresses that reduce their relative yields. Soil salinity is abiotic stress factor that poses a serious threat to agricultural production, as more than 800 million hectares of land in the world have been salt affected, which account for 6% of the total land area. Impacts of climatic and anthropogenic factors such as soil salinity, soil acidity and depletion of soil organic matter leading to reduced soil productivity continue to be a constraint and will become more serious under climate change and variability. Understanding the molecular basis of salinity will be helpful in developing selection strategies for improving salinity tolerance. To determine gene function relationships, large scale genome-wide reverse genetics approaches have been developed which includes both non-transgenic technology platforms such as TILLING (targeting induced local lesions in genomes) and EcoTILLING and insertional mutagenesis systems...
During postharvest life, broccoli suffers from floret yellowing confining its economic and nutrit... more During postharvest life, broccoli suffers from floret yellowing confining its economic and nutritional value. The objective of the present study was to explore the mechanisms employed by phytosulfokine α (PSKα) at 150 nM for delaying floret yellowing in broccoli during storage at 4°C for 28 days. Our results showed that the higher endogenous accumulation of hydrogen sulfide (H2S) resulting from the higher gene expression and activities of l-cysteine desulfhydrase (LCD) and d-cysteine desulfhydrase (DCD) in broccoli floret treated with 150 nM PSKα may serve as an endogenous signaling molecule for delaying senescence. Moreover, the suppressed ethylene biosynthesis in broccoli floret treated with 150 nM PSKα might be ascribed to lower gene expression and activities of ACC synthase (ACS) and ACC oxidase (ACO). Furthermore, lower gene expression and activities of Mg2+ dechelatase (MDC), pheophytinase (PPH), and pheophorbide a oxygenase (PaO) might be the reasons for the higher accumulati...
Wheat is one of the most important cereals, which is very valuable in food. Haploid plants are of... more Wheat is one of the most important cereals, which is very valuable in food. Haploid plants are of particular importance in plant breeding. The wheat seeds produced in the crosses between wheat and maize in the chromosome elimination method without of endosperm and are immature embryo, to prevent the abortions haploid embryos, they must have embryo rescue. Increasing production of haploid plants from produced embryos can improve production efficiency. In this study, With attention their effects gibberellic acid and 2,4-Dichlorophenoxyacetic acid on growth, cell size and cell division, Their use in the production of wheat haploid plant were studied. There was a significant difference at level 1% between the not use and use of gibberellic acid in difference times in the production of haploid from embryos, So that the most haploid plant produced in the use of gibberellic acid in the 4 days after pollination. Also, the use of 2,4-Dichlorophenoxyacetic acid in tiller maintenance liquid cu...
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