Introduction: Social acceptance is defined as the support by social network for patients via fami... more Introduction: Social acceptance is defined as the support by social network for patients via family, friends and colleagues that often reason the better management and prognosis. If the social acceptance is significantly low in patients with chronic diseases like tuberculosis, an appropriate plan can raise the level of knowledge and the culture of the community to enhance the level of patient and community's acceptance. The authors aim to evaluate social acceptance and related factors in these patients in Rafsanjan. Methods: This was a descriptive and cross-sectional study. It was conducted on 29 pulmonary TB (tuberculosis) patients who had referred to Rafsanjan city health center in Iran in 2014. They were selected by census method. The Marlou-Crown questionnaire was completed by patients, and the scores were calculated based on the questionnaire key using SPSS 16. T-test, Fisher and chi-square tests were used for evaluation, and the significance level was P-value <0.05. Res...
Objective: Uropathogenic Escherichia coli is known to cause urinary tract infections, and the end... more Objective: Uropathogenic Escherichia coli is known to cause urinary tract infections, and the endotoxin (lipopolysaccharide [LPS]) of this bacterium may cause deficiencies of sperm quality and morphology. In the present study, the effects of LPS on mouse sperm were studied, and the levels of interleukin (IL)-17A and possible changes in testis tissue were evaluated.Methods: LPS of uropathogenic E. coli was extracted using the methanol-chloroform method, followed confirmation using sodium dodecyl sulfate-polyacrylamide electrophoresis. Purified LPS (100 µg/kg) or phosphate-buffered saline was injected intraperitoneally into BALB/c mice for 7 days consecutively in the test and control groups, Mice were sacrificed on days 3, 7, and 42 after the first injection. Blood was tested for levels of IL-17A using the enzyme-linked immunosorbent assay method. Testis tissue and sperm were collected from each mouse and were studied according to standard protocols. Results: The mean sperm count and ...
Introduction: In recent decades, with the emergence of new industrial activities and increase pro... more Introduction: In recent decades, with the emergence of new industrial activities and increase production of waste production, industries require the existence of treatment equipment and sanitation. These devices sometimes spread infectious microorganisms into the air. These include waste recycling industries and wastewater treatment plants. Bioaerosols are produced at different stages of the wastewater treatment process, especially in processes such as mobile mechanisms and aeration. Methods: In this study, air sampling was performed from different units of the treatment plant and indoor air. the plates were placed in an incubator for 24-48 hours at a temperature of 35 to 37 ° C. Then the number of bacterial colonies grown on the culture media was counted and recorded as CFU/m3. Results: The results showed that the average population of bacterial aerosols was higher in summer than in spring. Also, the highest bacterial population was observed in spring in indoor air with an average ...
مقدمه: استرپتوکوکوس آگالاکتیه (GBS یا Group B streptococcus) باکتری است که به طور معمول در واژن، ... more مقدمه: استرپتوکوکوس آگالاکتیه (GBS یا Group B streptococcus) باکتری است که به طور معمول در واژن، مجاری ادراری و دستگاه گوارش وجود دارد. كلونیزه شدن مادر با GBS تهدید کننده‌ی نوزاد به عفونت‌های اکتسابی از قبیل مننژیت، باکتریمی و پنومونی می‌باشد. مطالعه‌ی حاضر با هدف تعیین مقاومت استرپتوکوکوس آگالاکتیه به آنتی‌بیوتیک‌های انتخابی و تشخیص ژن‌های مقاوم به اریترومایسین از نمونه‌های جدا شده از زنان حامل در شهر یزد انجام شد. روش‌ها: این مطالعه، از نوع توصیفی- تحلیلی بود که در آن، تعداد 100 نمونه‌ی باکتری GBS جهت بررسی فنوتیپی مقاومت به آنتی‌بیوتیک‌های پنی‌سیلین، اریترومایسین، کلیندامایسین و تتراسیکلین به روش Kirby–Bauer (دیسک دیفیوژن) مورد بررسی قرار گرفتند و بررسی ژنوتیپی مقاومت به اریترومایسین از نظر ژن‌های erm A، erm B و mef A با روش مولکولی انجام شد. یافته‌ها: بیشترین مقاومت مربوط به آنتی‌بیوتیک تتراسیکلین و کمترین مقاومت مربوط به پنی‌سیلین بود. مقاومت به اریترومایسین 8 درصد، کلیندامایسین 10 درصد، تتراسیکلین 95 درصد و پنی‌سیلین 1 درصد بود. از نظر ژنوتیپی، ژن‌های erm B (23 درصد) و erm A ...
Multiplex PCR Pregnant women Serotyping Streptococcus agalactiae Urine GBS Vaginal GBS Background... more Multiplex PCR Pregnant women Serotyping Streptococcus agalactiae Urine GBS Vaginal GBS Background and Aims: Group B streptococcus (GBS), is a bacterium that colonize in the vagina and/or rectum of pregnant, as well as non-pregnant women. The frequency of GBS varies in different geographical areas. Capsular serotyping of the bacterium could result in efficient vaccine designation. Serotyping data of GBS in Iranian pregnant women is limited. The aim of this study was to investigate the GBS molecular capsular serotyping of pregnant women in Yazd, Iran. Materials and Methods: In this cross-sectional study, a total of 346 vaginal and urine samples collected from pregnant women were cultured on blood agar and following incubation, the suspected colonies were identified according to standard protocol. Capsular serotyping was carried out by multiplex-polymerase chain reaction assay. Results: Three hundred forty six samples were collected from pregnant women out of which 57 (16.47%) and 33 (...
Genotyping Multiplex PCR Pregnant women Streptococcus agalactiae Background and Aims: Group B str... more Genotyping Multiplex PCR Pregnant women Streptococcus agalactiae Background and Aims: Group B streptococcus (GBS) may cause neonatal infection during and or after the delivery, and is the leading cause of sepsis, bacteremia, pneumonia and meningitis. The virulence factors are carried by both capsule and surface proteins by which serotypes and genotypes are determined. However, some genotypes are believed to be related in severity of neonatal diseases, therefor this investigation was aimed to determine the surface proteins genotype of detected GBS from both vagina and urine of pregnant women. Materials and Methods: In the present study, a total of 346 vaginal and urine samples were obtained from the same pregnant women. Following culturing the samples on sheep blood agar medium and related tests (CAMP, Hiporate hydrolysis) the suspected colonies were further confirmed by polymerase chain reaction (PCR) technique. The detected GBS species were then genotyped using multiplex PCR assay....
Introduction: Healthcare- Associated Infections (HAI) are known to be one of the most important h... more Introduction: Healthcare- Associated Infections (HAI) are known to be one of the most important health issues in developed and developing countries. The most common infections include central line-associated bloodstream infections, catheter-associated urinary tract infections, ventilator-associated pneumonia and surgical site infection. The aim of this study was to investigate the incidence of nosocomial infections in the elderly patients. Methods: In this cross-sectional study, 1279 patients were 60 years of age or older. Patients who had been admitted for more than 48 hours in the hospital and had no signs of infection at the time of admission, were entered into the study. It was evaluated four most common HAI, according to CDC include bacteremia, central line-associated blood stream infections, urinary tract infections, and ventilator-associated pneumonia. Infections may also occur at surgery sites, known as surgical site infections. The Chi-square and T- test or analysis of vari...
1Associate Professor of Microbiology, Dept. of Microbiology, School of Medicine, Shahid Sadoughi ... more 1Associate Professor of Microbiology, Dept. of Microbiology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran 2PhD student, MSc of Microbiology, Dept. of Laboratory Sciences, School of Paramedicine, Shahid Sadoughi University of Medical Science, Yazd, Iran 3Assistant Professor of Pathology, Dept. of Laboratory Sciences, School of Paramedicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
Journal of Social Behavior and Community Health, 2021
The etiology of suicide among students around the world revolves issues such as communication, ed... more The etiology of suicide among students around the world revolves issues such as communication, education, family problems and parents’ relationship. We intend to report a case with serious recurrent suicide attempt by gun shot during COVID-19 period. We also present psychiatric and clinical evaluation, cosmetic surgery consultation, as well as microbial assessments in this survey. The seventeen-year-old single male student from southwest of Iran with asymmetric deformed face was observed with the loss of parts of the mouse, nose, chin and mandible region .He had experienced a serious suicide attempt by a threatening plan following rejection. The patient had blurred, hypo phonic and hypotonic speech. Following the shooting, the soft and hard palate, facial, frontal bone and sinus had been destroyed and a foreign body was seen in the frontal sinus. After suicide risk as assessment, It was detected multiple predictive risk factors for recent suicide attempt and the possibility of recu...
Background and Objectives: Due to the important role of Streptococcus agalactiae, Group B strepto... more Background and Objectives: Due to the important role of Streptococcus agalactiae, Group B streptococci (GBS), in production of invasive disease in neonates, investigation regarding the pathogenicity and antibiotic resistance factors is necessary in selecting the appropriate therapeutic agents. Beside capsule, the pilus has been currently recognized as an important factor in enhancing the pathogenicity of GBS. Resistance of GBS to selected antibiotics is noticeably increasing which is mainly due to the anomalous use of these drugs for treatment. The aim of this study was to determine the prevalence of pili genes followed by antibiotic susceptibility of GBS, previously serotyped, isolated from pregnant women in the city of Yazd, Iran. Materials and Methods: Fifty seven GBS from pregnant women were subjected to multiplex PCR for determination of PI-1, PI-2a and PI-2b pilus-islands and simultaneously, the phenotype of antibiotic resistance to penicillin, tetracycline, erythromycin, clin...
Consequence of Streptococcus agalactiae, Group B Streptococcus (GBS) relating infant’s diseases a... more Consequence of Streptococcus agalactiae, Group B Streptococcus (GBS) relating infant’s diseases are well documented. Although many women carry this bacterium in their vagina, they may transfer to their infant during delivery and may result in different neonatal invasive diseases. The aim of this study was to determine the prevalence of GBS and serotyping the isolated species among un-selective non-pregnant women who attended two gynecology clinics in Tehran. In this cross-sectional study, a total of 560 vaginal samples collected from non-pregnant women. Following inoculation of the specimen on Blood Agar, the standard technology was applied for the final identification of GBS. Detected GBS species were further confirmed using specific PCR directed on dlts gene. Capsular serotyping was done by using the multiplex PCR method. The chi-square method was used for statistical analysis. Fifty (8.9%) out of 560 non-pregnant women were carriers of GBS. The most common types were III (36%), f...
Context: Group B Streptococcus has the capacity of being colonized in the rectovaginal organ of w... more Context: Group B Streptococcus has the capacity of being colonized in the rectovaginal organ of women and causes infections in a mother and her fetus, thereby leading to neonatal diseases. Evidence Acquisition: The aim of this review was to summarize all of the relevant articles published to highlight the prevalence of group B Streptococcus colonization or infection in different regions of Iran. A systematic literature review was conducted by searching PubMed, Scopus, Web of Science (ISI), ScienceDirect, Google Scholar, and domestic databases for papers published in English or Persian from 1992 up to July 2019, concerning the prevalence of group B Streptococcus among Iranian women. All information regarding year, location of cases, frequency, author’s name, date of publication, participants, pregnancy period, sampling, and quality assessment were recorded. Summary effects were derived using the random effects model. Results: Among 61 suitable papers, data revealed that 36,807 cases ...
Introduction: Social acceptance is defined as the support by social network for patients via fami... more Introduction: Social acceptance is defined as the support by social network for patients via family, friends and colleagues that often reason the better management and prognosis. If the social acceptance is significantly low in patients with chronic diseases like tuberculosis, an appropriate plan can raise the level of knowledge and the culture of the community to enhance the level of patient and community's acceptance. The authors aim to evaluate social acceptance and related factors in these patients in Rafsanjan. Methods: This was a descriptive and cross-sectional study. It was conducted on 29 pulmonary TB (tuberculosis) patients who had referred to Rafsanjan city health center in Iran in 2014. They were selected by census method. The Marlou-Crown questionnaire was completed by patients, and the scores were calculated based on the questionnaire key using SPSS 16. T-test, Fisher and chi-square tests were used for evaluation, and the significance level was P-value <0.05. Res...
Objective: Uropathogenic Escherichia coli is known to cause urinary tract infections, and the end... more Objective: Uropathogenic Escherichia coli is known to cause urinary tract infections, and the endotoxin (lipopolysaccharide [LPS]) of this bacterium may cause deficiencies of sperm quality and morphology. In the present study, the effects of LPS on mouse sperm were studied, and the levels of interleukin (IL)-17A and possible changes in testis tissue were evaluated.Methods: LPS of uropathogenic E. coli was extracted using the methanol-chloroform method, followed confirmation using sodium dodecyl sulfate-polyacrylamide electrophoresis. Purified LPS (100 µg/kg) or phosphate-buffered saline was injected intraperitoneally into BALB/c mice for 7 days consecutively in the test and control groups, Mice were sacrificed on days 3, 7, and 42 after the first injection. Blood was tested for levels of IL-17A using the enzyme-linked immunosorbent assay method. Testis tissue and sperm were collected from each mouse and were studied according to standard protocols. Results: The mean sperm count and ...
Introduction: In recent decades, with the emergence of new industrial activities and increase pro... more Introduction: In recent decades, with the emergence of new industrial activities and increase production of waste production, industries require the existence of treatment equipment and sanitation. These devices sometimes spread infectious microorganisms into the air. These include waste recycling industries and wastewater treatment plants. Bioaerosols are produced at different stages of the wastewater treatment process, especially in processes such as mobile mechanisms and aeration. Methods: In this study, air sampling was performed from different units of the treatment plant and indoor air. the plates were placed in an incubator for 24-48 hours at a temperature of 35 to 37 ° C. Then the number of bacterial colonies grown on the culture media was counted and recorded as CFU/m3. Results: The results showed that the average population of bacterial aerosols was higher in summer than in spring. Also, the highest bacterial population was observed in spring in indoor air with an average ...
مقدمه: استرپتوکوکوس آگالاکتیه (GBS یا Group B streptococcus) باکتری است که به طور معمول در واژن، ... more مقدمه: استرپتوکوکوس آگالاکتیه (GBS یا Group B streptococcus) باکتری است که به طور معمول در واژن، مجاری ادراری و دستگاه گوارش وجود دارد. كلونیزه شدن مادر با GBS تهدید کننده‌ی نوزاد به عفونت‌های اکتسابی از قبیل مننژیت، باکتریمی و پنومونی می‌باشد. مطالعه‌ی حاضر با هدف تعیین مقاومت استرپتوکوکوس آگالاکتیه به آنتی‌بیوتیک‌های انتخابی و تشخیص ژن‌های مقاوم به اریترومایسین از نمونه‌های جدا شده از زنان حامل در شهر یزد انجام شد. روش‌ها: این مطالعه، از نوع توصیفی- تحلیلی بود که در آن، تعداد 100 نمونه‌ی باکتری GBS جهت بررسی فنوتیپی مقاومت به آنتی‌بیوتیک‌های پنی‌سیلین، اریترومایسین، کلیندامایسین و تتراسیکلین به روش Kirby–Bauer (دیسک دیفیوژن) مورد بررسی قرار گرفتند و بررسی ژنوتیپی مقاومت به اریترومایسین از نظر ژن‌های erm A، erm B و mef A با روش مولکولی انجام شد. یافته‌ها: بیشترین مقاومت مربوط به آنتی‌بیوتیک تتراسیکلین و کمترین مقاومت مربوط به پنی‌سیلین بود. مقاومت به اریترومایسین 8 درصد، کلیندامایسین 10 درصد، تتراسیکلین 95 درصد و پنی‌سیلین 1 درصد بود. از نظر ژنوتیپی، ژن‌های erm B (23 درصد) و erm A ...
Multiplex PCR Pregnant women Serotyping Streptococcus agalactiae Urine GBS Vaginal GBS Background... more Multiplex PCR Pregnant women Serotyping Streptococcus agalactiae Urine GBS Vaginal GBS Background and Aims: Group B streptococcus (GBS), is a bacterium that colonize in the vagina and/or rectum of pregnant, as well as non-pregnant women. The frequency of GBS varies in different geographical areas. Capsular serotyping of the bacterium could result in efficient vaccine designation. Serotyping data of GBS in Iranian pregnant women is limited. The aim of this study was to investigate the GBS molecular capsular serotyping of pregnant women in Yazd, Iran. Materials and Methods: In this cross-sectional study, a total of 346 vaginal and urine samples collected from pregnant women were cultured on blood agar and following incubation, the suspected colonies were identified according to standard protocol. Capsular serotyping was carried out by multiplex-polymerase chain reaction assay. Results: Three hundred forty six samples were collected from pregnant women out of which 57 (16.47%) and 33 (...
Genotyping Multiplex PCR Pregnant women Streptococcus agalactiae Background and Aims: Group B str... more Genotyping Multiplex PCR Pregnant women Streptococcus agalactiae Background and Aims: Group B streptococcus (GBS) may cause neonatal infection during and or after the delivery, and is the leading cause of sepsis, bacteremia, pneumonia and meningitis. The virulence factors are carried by both capsule and surface proteins by which serotypes and genotypes are determined. However, some genotypes are believed to be related in severity of neonatal diseases, therefor this investigation was aimed to determine the surface proteins genotype of detected GBS from both vagina and urine of pregnant women. Materials and Methods: In the present study, a total of 346 vaginal and urine samples were obtained from the same pregnant women. Following culturing the samples on sheep blood agar medium and related tests (CAMP, Hiporate hydrolysis) the suspected colonies were further confirmed by polymerase chain reaction (PCR) technique. The detected GBS species were then genotyped using multiplex PCR assay....
Introduction: Healthcare- Associated Infections (HAI) are known to be one of the most important h... more Introduction: Healthcare- Associated Infections (HAI) are known to be one of the most important health issues in developed and developing countries. The most common infections include central line-associated bloodstream infections, catheter-associated urinary tract infections, ventilator-associated pneumonia and surgical site infection. The aim of this study was to investigate the incidence of nosocomial infections in the elderly patients. Methods: In this cross-sectional study, 1279 patients were 60 years of age or older. Patients who had been admitted for more than 48 hours in the hospital and had no signs of infection at the time of admission, were entered into the study. It was evaluated four most common HAI, according to CDC include bacteremia, central line-associated blood stream infections, urinary tract infections, and ventilator-associated pneumonia. Infections may also occur at surgery sites, known as surgical site infections. The Chi-square and T- test or analysis of vari...
1Associate Professor of Microbiology, Dept. of Microbiology, School of Medicine, Shahid Sadoughi ... more 1Associate Professor of Microbiology, Dept. of Microbiology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran 2PhD student, MSc of Microbiology, Dept. of Laboratory Sciences, School of Paramedicine, Shahid Sadoughi University of Medical Science, Yazd, Iran 3Assistant Professor of Pathology, Dept. of Laboratory Sciences, School of Paramedicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
Journal of Social Behavior and Community Health, 2021
The etiology of suicide among students around the world revolves issues such as communication, ed... more The etiology of suicide among students around the world revolves issues such as communication, education, family problems and parents’ relationship. We intend to report a case with serious recurrent suicide attempt by gun shot during COVID-19 period. We also present psychiatric and clinical evaluation, cosmetic surgery consultation, as well as microbial assessments in this survey. The seventeen-year-old single male student from southwest of Iran with asymmetric deformed face was observed with the loss of parts of the mouse, nose, chin and mandible region .He had experienced a serious suicide attempt by a threatening plan following rejection. The patient had blurred, hypo phonic and hypotonic speech. Following the shooting, the soft and hard palate, facial, frontal bone and sinus had been destroyed and a foreign body was seen in the frontal sinus. After suicide risk as assessment, It was detected multiple predictive risk factors for recent suicide attempt and the possibility of recu...
Background and Objectives: Due to the important role of Streptococcus agalactiae, Group B strepto... more Background and Objectives: Due to the important role of Streptococcus agalactiae, Group B streptococci (GBS), in production of invasive disease in neonates, investigation regarding the pathogenicity and antibiotic resistance factors is necessary in selecting the appropriate therapeutic agents. Beside capsule, the pilus has been currently recognized as an important factor in enhancing the pathogenicity of GBS. Resistance of GBS to selected antibiotics is noticeably increasing which is mainly due to the anomalous use of these drugs for treatment. The aim of this study was to determine the prevalence of pili genes followed by antibiotic susceptibility of GBS, previously serotyped, isolated from pregnant women in the city of Yazd, Iran. Materials and Methods: Fifty seven GBS from pregnant women were subjected to multiplex PCR for determination of PI-1, PI-2a and PI-2b pilus-islands and simultaneously, the phenotype of antibiotic resistance to penicillin, tetracycline, erythromycin, clin...
Consequence of Streptococcus agalactiae, Group B Streptococcus (GBS) relating infant’s diseases a... more Consequence of Streptococcus agalactiae, Group B Streptococcus (GBS) relating infant’s diseases are well documented. Although many women carry this bacterium in their vagina, they may transfer to their infant during delivery and may result in different neonatal invasive diseases. The aim of this study was to determine the prevalence of GBS and serotyping the isolated species among un-selective non-pregnant women who attended two gynecology clinics in Tehran. In this cross-sectional study, a total of 560 vaginal samples collected from non-pregnant women. Following inoculation of the specimen on Blood Agar, the standard technology was applied for the final identification of GBS. Detected GBS species were further confirmed using specific PCR directed on dlts gene. Capsular serotyping was done by using the multiplex PCR method. The chi-square method was used for statistical analysis. Fifty (8.9%) out of 560 non-pregnant women were carriers of GBS. The most common types were III (36%), f...
Context: Group B Streptococcus has the capacity of being colonized in the rectovaginal organ of w... more Context: Group B Streptococcus has the capacity of being colonized in the rectovaginal organ of women and causes infections in a mother and her fetus, thereby leading to neonatal diseases. Evidence Acquisition: The aim of this review was to summarize all of the relevant articles published to highlight the prevalence of group B Streptococcus colonization or infection in different regions of Iran. A systematic literature review was conducted by searching PubMed, Scopus, Web of Science (ISI), ScienceDirect, Google Scholar, and domestic databases for papers published in English or Persian from 1992 up to July 2019, concerning the prevalence of group B Streptococcus among Iranian women. All information regarding year, location of cases, frequency, author’s name, date of publication, participants, pregnancy period, sampling, and quality assessment were recorded. Summary effects were derived using the random effects model. Results: Among 61 suitable papers, data revealed that 36,807 cases ...
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