Current studies have provided evidence that exposure of renal epithelial cells to oxalate and cal... more Current studies have provided evidence that exposure of renal epithelial cells to oxalate and calcium oxalate crystals induces lipid peroxidation and injures the cells. Since oxidant/antioxidant balance is likely to play a critical role, we determined the effect of antioxidant scavengers on production of free radicals and injury to LLC-PK1 and MDCK cells from exposure to oxalate (Ox) or Ox + calcium oxalate monohydrate (COM) crystals.LLC-PK1 and MDCK cells were grown in monolayers and exposed to 1.0 mmol. Ox or 1.0 mmol. Ox + 500 μg./ml. COM crystals for 120 or 240 minutes. We measured the release of lactate dehydrogenase (LDH) as a marker for cell injury and malondialdehyde (MDA) as a marker of lipid peroxidation. Superoxide and hydroxyl radicals were measured in the presence or absence of 400 U/ml. catalase, or superoxide dismutase (SOD).Exposure of LLC-PK1 cells to Ox resulted in a significant increase in MDA and release of LDH, which was further elevated when COM crystals were added. MDCK cells responded similarly to both challenges, but showed significantly less impact when compared with LLC-PK1 cells. Both treatments were associated with significant increase in the generation of hydroxyl and superoxide radicals by both cell types. In both cell lines, the addition of catalase or SOD significantly reduced the increase of MDA and release of LDH.Results of the present study indicate that both Ox and COM crystals are injurious to renal epithelial cells and the injury is associated with generation of free radicals. Cells of proximal tubular origin are more susceptible than those of distal tubules and collecting ducts. Free radical scavengers, catalase and SOD provide significant protection.
... Find all citations by this author (default). Or filter your current search. Khan SR. Departme... more ... Find all citations by this author (default). Or filter your current search. Khan SR. Department of Pathology, College of Medicine, University of Florida, Gainesville 32610-0275, USA. Find all citations in this journal (default). Or filter ...
Page 80. Renal Cellular Dysfunction/Damage and the Formation of Kidney Stones Saeed R. Khan 5 Abs... more Page 80. Renal Cellular Dysfunction/Damage and the Formation of Kidney Stones Saeed R. Khan 5 Abstract Supersaturation is the driving force behind crystal formation in the kidneys. It can, however, result only in the formation of crystals that can often be harmlessly excreted. ...
... SPORE ORNAMENTATION OF NORTH AMERICAN PHELLODON SPECIES1 RICHARD E. BAIRD AND SAEED R. KHAN .... more ... SPORE ORNAMENTATION OF NORTH AMERICAN PHELLODON SPECIES1 RICHARD E. BAIRD AND SAEED R. KHAN ... Har-rison placed Bankera and Phellodon in the Hyd-naceae with the family name based on Hydnum (=Sarcodon). ...
... Find all citations by this author (default). Or filter your current search. Khan SR. Departme... more ... Find all citations by this author (default). Or filter your current search. Khan SR. Department of Pathology, College of Medicine, University of Florida, Gainesville 32610-0275, USA. Find all citations in this journal (default). Or filter ...
Glutamine may be an essential dietary component, especially for the support of intestinal mucosal... more Glutamine may be an essential dietary component, especially for the support of intestinal mucosal growth and function. This study evaluated the effects of a glutamine-enriched elemental diet, administered before whole-abdominal radiation on gut glutamine metabolism, mucosal morphometrics, and bacterial translocation. Rats were randomized to receive a nutritionally complete elemental diet that was glutamine-enriched or glutamine-free for 4 days. The animals were then subjected to a single dose of 1000 cGy x-radiation to the abdomen. After irradiation, all animals received the glutamine-free diet. Four days later the animals underwent laparotomy for sampling of arterial and portal venous blood, culture of mesenteric lymph nodes, and removal of the small intestine for microscopic examination. There was no difference in arterial glutamine or gut glutamine extraction between the two groups, but body weight loss was significantly diminished in the glutamine-fed rats. Rats receiving the glutamine-enriched elemental diet before radiation had a significant increase in jejunal villous number, villous height, and number of metaphase mitoses per crypt. Scanning electron microscopy confirmed the presence of an intact gut epithelium in eight of eight rats receiving prophylactic glutamine compared to one of eight animals in the glutamine-free group. Three of eight rats fed glutamine had culture positive mesenteric lymph nodes compared with five of seven rats receiving the glutamine-free diet. Glutamine exerts a protective effect on the small bowel mucosa by supporting crypt cell proliferation effect on accelerate healing of the acutely radiated bowel.
Advertisement. Close Window. Close Window. Thank you for choosing to subscribe to the eTOC for Cu... more Advertisement. Close Window. Close Window. Thank you for choosing to subscribe to the eTOC for Current Opinion in Urology. Enter your Email address: Wolters Kluwer Health may email you for journal alerts and information ...
Current studies have provided evidence that exposure of renal epithelial cells to oxalate and cal... more Current studies have provided evidence that exposure of renal epithelial cells to oxalate and calcium oxalate crystals induces lipid peroxidation and injures the cells. Since oxidant/antioxidant balance is likely to play a critical role, we determined the effect of antioxidant scavengers on production of free radicals and injury to LLC-PK1 and MDCK cells from exposure to oxalate (Ox) or Ox + calcium oxalate monohydrate (COM) crystals.LLC-PK1 and MDCK cells were grown in monolayers and exposed to 1.0 mmol. Ox or 1.0 mmol. Ox + 500 μg./ml. COM crystals for 120 or 240 minutes. We measured the release of lactate dehydrogenase (LDH) as a marker for cell injury and malondialdehyde (MDA) as a marker of lipid peroxidation. Superoxide and hydroxyl radicals were measured in the presence or absence of 400 U/ml. catalase, or superoxide dismutase (SOD).Exposure of LLC-PK1 cells to Ox resulted in a significant increase in MDA and release of LDH, which was further elevated when COM crystals were added. MDCK cells responded similarly to both challenges, but showed significantly less impact when compared with LLC-PK1 cells. Both treatments were associated with significant increase in the generation of hydroxyl and superoxide radicals by both cell types. In both cell lines, the addition of catalase or SOD significantly reduced the increase of MDA and release of LDH.Results of the present study indicate that both Ox and COM crystals are injurious to renal epithelial cells and the injury is associated with generation of free radicals. Cells of proximal tubular origin are more susceptible than those of distal tubules and collecting ducts. Free radical scavengers, catalase and SOD provide significant protection.
... Find all citations by this author (default). Or filter your current search. Khan SR. Departme... more ... Find all citations by this author (default). Or filter your current search. Khan SR. Department of Pathology, College of Medicine, University of Florida, Gainesville 32610-0275, USA. Find all citations in this journal (default). Or filter ...
Page 80. Renal Cellular Dysfunction/Damage and the Formation of Kidney Stones Saeed R. Khan 5 Abs... more Page 80. Renal Cellular Dysfunction/Damage and the Formation of Kidney Stones Saeed R. Khan 5 Abstract Supersaturation is the driving force behind crystal formation in the kidneys. It can, however, result only in the formation of crystals that can often be harmlessly excreted. ...
... SPORE ORNAMENTATION OF NORTH AMERICAN PHELLODON SPECIES1 RICHARD E. BAIRD AND SAEED R. KHAN .... more ... SPORE ORNAMENTATION OF NORTH AMERICAN PHELLODON SPECIES1 RICHARD E. BAIRD AND SAEED R. KHAN ... Har-rison placed Bankera and Phellodon in the Hyd-naceae with the family name based on Hydnum (=Sarcodon). ...
... Find all citations by this author (default). Or filter your current search. Khan SR. Departme... more ... Find all citations by this author (default). Or filter your current search. Khan SR. Department of Pathology, College of Medicine, University of Florida, Gainesville 32610-0275, USA. Find all citations in this journal (default). Or filter ...
Glutamine may be an essential dietary component, especially for the support of intestinal mucosal... more Glutamine may be an essential dietary component, especially for the support of intestinal mucosal growth and function. This study evaluated the effects of a glutamine-enriched elemental diet, administered before whole-abdominal radiation on gut glutamine metabolism, mucosal morphometrics, and bacterial translocation. Rats were randomized to receive a nutritionally complete elemental diet that was glutamine-enriched or glutamine-free for 4 days. The animals were then subjected to a single dose of 1000 cGy x-radiation to the abdomen. After irradiation, all animals received the glutamine-free diet. Four days later the animals underwent laparotomy for sampling of arterial and portal venous blood, culture of mesenteric lymph nodes, and removal of the small intestine for microscopic examination. There was no difference in arterial glutamine or gut glutamine extraction between the two groups, but body weight loss was significantly diminished in the glutamine-fed rats. Rats receiving the glutamine-enriched elemental diet before radiation had a significant increase in jejunal villous number, villous height, and number of metaphase mitoses per crypt. Scanning electron microscopy confirmed the presence of an intact gut epithelium in eight of eight rats receiving prophylactic glutamine compared to one of eight animals in the glutamine-free group. Three of eight rats fed glutamine had culture positive mesenteric lymph nodes compared with five of seven rats receiving the glutamine-free diet. Glutamine exerts a protective effect on the small bowel mucosa by supporting crypt cell proliferation effect on accelerate healing of the acutely radiated bowel.
Advertisement. Close Window. Close Window. Thank you for choosing to subscribe to the eTOC for Cu... more Advertisement. Close Window. Close Window. Thank you for choosing to subscribe to the eTOC for Current Opinion in Urology. Enter your Email address: Wolters Kluwer Health may email you for journal alerts and information ...
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