International Journal of Pharmacy and Pharmaceutical Sciences, 2015
Objective: Anticancer activity of methanol extract of Hippophae salicifolia (MEHS) bark was evalu... more Objective: Anticancer activity of methanol extract of Hippophae salicifolia (MEHS) bark was evaluated using the Ehrlich Ascites Carcinoma (EAC) cells on Swiss albino mice. Methods: In vitro cytotoxicity assay has been evaluated by using the trypan blue and MTT assay method. The determination of in vivo anticancer activity was performed using EAC cells (2 × 10 6 ) induced mice groups (n = 12). After treatment with MEHS at the doses of 50 and 100 mg/kg b. w. respectively for 9 days, half of the mice of each group were sacrificed and the rest were kept for increase in life span determination. The anticancer potential of MEHS was assessed by evaluating tumor volume, viable and nonviable tumor cell count, tumor weight, hematological parameters and biochemical estimations. Furthermore, tissue antioxidant parameters were checked from liver homogenate. Results: In MEHS treated groups (50 and 100 mg/kg b. w.) tumor volume, tumor weight, viable cell count was significantly decreased as compar...
This research was conducted to evaluate the possible antihelmintic activity of leaf and stem of C... more This research was conducted to evaluate the possible antihelmintic activity of leaf and stem of Carica papaya as a potent remedy in both human and veterinary practices using Indian adult earth worm (Pheretima posthuma) as a test worm. Various concentration (5%, 2.5%, 1%) were invitro tested using different extracts like ( hydroalcoholic, chloroform) and the results were involved in terms of time for paralysis (P) and time for death (D). It showed shortest time for paralysis in 5% concentration while the time for paralysis and death will increase in 2.5% and 1% respectively as compared to standard drug Albendazole. The present study indicated that the leaf and stem of Carica papaya has a significant anthelmintic activity and can be a potent drug due to, cost benefit and easy availability.
Journal of Planar Chromatography - Modern TLC, 2018
Asparagus racemosus has been gaining importance due to its recently detected pharmacological acti... more Asparagus racemosus has been gaining importance due to its recently detected pharmacological activities. There has been a need to develop a simple isolation method for the major bioactive phytochemical and its quantitative evaluation in the herb. Hence, a rapid, sensitive, and reproducible high-performance thin-layer chromatography (HPTLC) method has been developed for determining its major bioactive marker, shatavarin IV. HPTLC silica gel F254 pre-coated plates were used with ethyl acetate–methanol–water (7.5:1.5:1, v/v) as the mobile phase. The plates were developed to a distance of 60 mm at 23 ± 4°C in twin-trough chamber, previously saturated for 5 min. Under these conditions, the retention factor (RF) of shatavarin IV was 0.43 and it was quantified at 425 nm, the wavelength of maximum absorbance after derivatization in anisaldehyde–H2SO4 reagent. Shatavarin IV showed a linear function of amount 600 to 1800 ng per band; the correlation coefficient was 0.9934, indicating a good relationship between peak area and amount. The limits of detection (LOD) and quantitation (LOQ) were 14.35 ng and 43.50 ng, respectively. The method was validated for precision, repeatability, and accuracy. Recovery was determined by spiking samples with shatavarin IV before extraction and it was found to be 96.17%. Shatavarin IV was found to be present in roots (0.40%) and in enriched fraction (61.20%).
The present study assessed the methanol extract of Streblus asper stem bark (MESA) for antitumor ... more The present study assessed the methanol extract of Streblus asper stem bark (MESA) for antitumor effect and antioxidant influence against Ehrlich ascites carcinoma (EAC) in Swiss albino mice. Twenty four hours after intraperitonial inoculation of tumor (EAC) cells in mice, MESA was administered at 200 and 400 mg/kg body weight daily for 9 consecutive days. On the 10 th day, half of the mice were sacrificed for estimation of tumor parameters, haematological, liver and kidney antioxidant parameters; and the rest were kept alive for assessment of survival. MESA exhibited dose dependent and significant (p < 0.01) decrease in tumor proliferation and extended the life span of EAC bearing mice. Hematological profiles were significantly (p < 0.01) restored near to normal in MESA treated mice as compared to EAC control. MESA treatment significantly (p < 0.01) modulated the hepatic and renal antioxidant parameters as compared to EAC control. The present study demonstrated that S. asp...
Objective: Evaluation of anti cancer activity of methanol extracts of Curcuma zedoaria against Eh... more Objective: Evaluation of anti cancer activity of methanol extracts of Curcuma zedoaria against Ehrlich’s ascities carcinoma (EAC) cell line in Swiss albino mice. Method: In vitro cytotoxicity assay has been evaluated by using the trypan blue and MTT assay method. In vivo anti cancer activity was performed by using EAC cells induced mice groups (n=12), at the doses of 100 and 200 mg/kg b.w. respectively, half of the mice were sacrificed and the rest were kept alive for life span parameter. The anti cancer potential of MECZ was assessed by evaluating tumor volume, viable and nonviable tumor cell count, tumor weight, hematological parameters and biochemical estimations. Furthermore, antioxidant parameters were assayed by estimating liver tissue enzymes. Result: Dose dependent cytotoxicity was observed in (* p < 0.05) Trypan blue and MTT assay method. In vivo anti cancer parameters like tumor volume, tumor weight, and viable cell count were decreased compared to the EAC control group...
ABSTRACTS Objective: Anticancer activity of methanol extract of Hippophae salicifolia (MEHS) bark... more ABSTRACTS Objective: Anticancer activity of methanol extract of Hippophae salicifolia (MEHS) bark was evaluated using the Ehrlich Ascites Carcinoma (EAC) cells on Swiss albino mice. Methods: In vitro cytotoxicity assay has been evaluated by using the trypan blue and MTT assay method. The determination of in vivo anticancer activity was performed using EAC cells (2 × 106 Results: In MEHS treated groups (50 and 100 mg/kg b. w.) tumor volume, tumor weight, viable cell count was significantly decreased as compared to that of the EAC control group. Life span increased by 34% and 43% in a dose dependant manner as compared to EAC control group. The hematological, biochemical and liver tissue antioxidant parameter are significantly (p < 0.05) restored towards the normal level after treatment with MEHS. ) induced mice groups (n = 12). After treatment with MEHS at the doses of 50 and 100 mg/kg b. w. respectively for 9 days, half of the mice of each group were sacrificed and the rest were k...
JPC – Journal of Planar Chromatography – Modern TLC
Herbal medicine is gaining its popularity day by day and gradually streaming toward integration i... more Herbal medicine is gaining its popularity day by day and gradually streaming toward integration into the mainstream healthcare systems. Therefore, standardization of herbal formulations is essential in order to assess quality of the drugs. Two commonly used paradigms, compound and pattern-based approaches, were performed to ensure quality and hence product consistency. A rapid, sensitive, and reproducible high-performance thin-layer chromatography (HPTLC) method has been developed for one of the major bioactive markers, piperine. The plates were developed using the mobile phase with toluene–ethyl acetate–formic acid–methanol (3:3:0.8:0.2, v/v). The method was validated for limit of detection, limit of quantification, linearity, specificity, precession, and recovery. The results indicate that the bioactive compound was present in 2.64% w/w in the fruit of Piper nigrum Linn. and 0.253% in a herbal formulation. Each ingredient was analyzed vis-a-vis the herbal formulation and the reflectance spectrum of the matching component(s) to ensure the presence of each ingredient in the herbal formulation. The individual component adding up has also resulted similar fingerprint pattern as that of herbal formulation. Thus, the TLC method could be a rapid tool for the delineation of each individual ingredient in the finished products for authenticity, efficacy, and for batch-to-batch consistency.
The present study evaluates the antioxidant effect of methanol extract of Hippophae salicifolia (... more The present study evaluates the antioxidant effect of methanol extract of Hippophae salicifolia (MEHS) bark with special emphasis on its role on oxidative DNA damage in mouse peritoneal macrophages. In vitro antioxidant activity was estimated by standard antioxidant assays whereas the antioxidant activity concluded the H(+) donating capacity. Mouse erythrocytes&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39; hemolysis and peritoneal macrophages&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39; DNA damage were determined spectrophotometrically. In vivo antioxidant activity of MEHS was determined in carbon tetrachloride-induced mice by studying its effect on superoxide anion production in macrophages cells, superoxide dismutase in the cell lysate, DNA damage, lipid peroxidation, and reduces glutathione. The extract showed good in vitro antioxidant activities whereas the inhibitory concentrations values ranged from 5.80 to 106.5 μg/ml. MEHS significantly (P &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.05) attenuated the oxidative DNA damage. It also attenuated the oxidative conversion of hemoglobin to methemoglobin and elevation of enzymatic and nonenzymatic antioxidant in cells. The result indicates MEHS has good in vitro-in vivo antioxidant property as well as the protective effect on DNA and red blood cell may be due to its H(+) donating property.
Many of the major chemotherapeutic agents are secondary metabolites found in nature. Zanthoxylum ... more Many of the major chemotherapeutic agents are secondary metabolites found in nature. Zanthoxylum alatum Roxb. (Rutaceae) is traditionally used in the treatment of various diseases. The present study evaluates the apoptotic activity of methanol extract of Z. alatum (MEZA) on Ehrlich ascites tumor (EAT) in Swiss albino mice. The presence of flavonoids in MEZA was standardized by HPLC. The in vitro cytotoxicity of MEZA was measured by the MTT assay. The in vivo antitumor activity of MEZA (100 and 200 mg/kg b.w., i.p. for 9 days) was also evaluated. On the 10th day, EAT tumor volume, cell viability, and hematological parameters were assayed. Apoptotic morphology was determined by acredine orange/ethedium bromide using fluorescence microscopy. Apoptosis percentage was measured by flow cytometric analysis using annexine-V-FITC. Also, DNA damage and bcl-2/bax were estimated by UV-method and western blot, respectively. HPLC analysis revealed presence of three flavonoids, rutin, myricetin, a...
Many of the major chemotherapeutic agents are secondary metabolites found in nature. Zanthoxylum ... more Many of the major chemotherapeutic agents are secondary metabolites found in nature. Zanthoxylum alatum Roxb. (Rutaceae) is traditionally used in the treatment of various diseases. The present study evaluates the apoptotic activity of methanol extract of Z. alatum (MEZA) on Ehrlich ascites tumor (EAT) in Swiss albino mice. The presence of flavonoids in MEZA was standardized by HPLC. The in vitro cytotoxicity of MEZA was measured by the MTT assay. The in vivo antitumor activity of MEZA (100 and 200 mg/kg b.w., i.p. for 9 days) was also evaluated. On the 10th day, EAT tumor volume, cell viability, and hematological parameters were assayed. Apoptotic morphology was determined by acredine orange/ethedium bromide using fluorescence microscopy. Apoptosis percentage was measured by flow cytometric analysis using annexine-V-FITC. Also, DNA damage and bcl-2/bax were estimated by UV-method and western blot, respectively. HPLC analysis revealed presence of three flavonoids, rutin, myricetin, and quercetin. MEZA showed satisfactory cytotoxicity in MTT assay (IC50 = 111.50 µg/ml). The extract significantly (p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.01) changed the tumor volume, viable, non-viable cell count, and hematological parameters towards the normal. Apoptotic activity of MEZA was confirmed by acridine orange/ethidium bromide staining, annexin-V-FITC staining, DNA fragmentation, and Bcl-2/Bax ratio. The study showed that MEZA has antitumor activity which may be due to the presence of flavonoids in the extract.
Journal of experimental therapeutics & oncology, 2013
The present study assessed the methanol extract of Streblus asper stem bark (MESA) for antitumor ... more The present study assessed the methanol extract of Streblus asper stem bark (MESA) for antitumor effect and antioxidant influence against Ehrlich ascites carcinoma (EAC) in Swiss albino mice. Twenty four hours after intraperitonial inoculation of tumor (EAC) cells in mice, MESA was administered at 200 and 400 mg/kg body weight daily for 9 consecutive days. On the 10 th day, half of the mice were sacrificed for estimation of tumor parameters, haematological, liver and kidney antioxidant parameters; and the rest were kept alive for assessment of survival. MESA exhibited dose dependent and significant (p < 0.01) decrease in tumor proliferation and extended the life span of EAC bearing mice. Hematological profiles were significantly (p < 0.01) restored near to normal in MESA treated mice as compared to EAC control. MESA treatment significantly (p < 0.01) modulated the hepatic and renal antioxidant parameters as compared to EAC control. The present study demonstrated that S. asp...
Journal of Environmental Pathology, Toxicology and Oncology, 2015
Methanol extracts of Thevetia peruviana (METP) (Apocynaceae) fruit showed antitumor activity agai... more Methanol extracts of Thevetia peruviana (METP) (Apocynaceae) fruit showed antitumor activity against Ehrlich&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;s ascites carcinoma (EAC) cell line in Swiss albino mice. The METP-treated group&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;s tumor volume, tumor weight, and viable cell count were decreased compared to the EAC control group. Tumor volumes were 3.62±0.12 ml, 2.88±0.23 ml, and 1.34±0.17 ml for the EAC control group and the METP-treated groups (50 mg/kg and 100 mg/kg body weight), respectively. Nonviable cell counts were 4.44%±0.42%, 18.57%±3.07%, and 68.12%±5.32% in the EAC control group and the METP-treated groups (50 mg/kg and 100 mg/kg body weight), respectively. METP-treated EAC cell-bearing mice had an increased life span (48.69% and 83.78%) compared to the EAC control group. Hematological and serum biochemical profiles were restored to normal levels in METP-treated mice compared to the EAC control group. METP significantly (P&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;0.001) decreased lipid peroxidation and recovered reduced glutathione, superoxide dismutase, and catalase toward normal levels compared to the EAC control group. In summary, METP exhibited remarkable antitumor activity in Swiss albino mice, which is plausibly attributable to its augmentation of endogenous antioxidant mechanisms.
Journal of Environmental Pathology, Toxicology and Oncology, 2014
In the present study, we evaluated the ameliorative potential of a citrus flavonoid, naringenin (... more In the present study, we evaluated the ameliorative potential of a citrus flavonoid, naringenin (NRG), against experimentally induced arsenic toxicity in Swiss albino mice. NRG (5 and 10 mg kg-l) was administered orally to mice 30 minutes before oral administration of arsenic trioxide (3 mg kg-l) for 14 consecutive days. On day 15, the following parameters were evaluated: body weight; organ weight; hematological profile; serum biochemical profile; hepatic and renal tissue antioxidative parameters including lipid peroxidation, reduced and oxidized glutathione, glutathione-S-transferase, glutathione peroxidase, glutathione reductase, superoxide dismutase, catalase levels; and DNA fragmentation. Co-treatment with NRG markedly and significantly normalized body weights, organ weights, hematological profiles, and serum biochemical profiles and significantly modulated all of the hepatic and renal tissue biochemical parameters and DNA fragmentation in arsenic-intoxicated mice. The present findings indicate that naringenin remarkably ameliorated the effects of arsenic-induced toxicity in albino mice due to its strong antioxidant property.
International Journal of Pharmacy and Pharmaceutical Sciences, 2015
Objective: Anticancer activity of methanol extract of Hippophae salicifolia (MEHS) bark was evalu... more Objective: Anticancer activity of methanol extract of Hippophae salicifolia (MEHS) bark was evaluated using the Ehrlich Ascites Carcinoma (EAC) cells on Swiss albino mice. Methods: In vitro cytotoxicity assay has been evaluated by using the trypan blue and MTT assay method. The determination of in vivo anticancer activity was performed using EAC cells (2 × 10 6 ) induced mice groups (n = 12). After treatment with MEHS at the doses of 50 and 100 mg/kg b. w. respectively for 9 days, half of the mice of each group were sacrificed and the rest were kept for increase in life span determination. The anticancer potential of MEHS was assessed by evaluating tumor volume, viable and nonviable tumor cell count, tumor weight, hematological parameters and biochemical estimations. Furthermore, tissue antioxidant parameters were checked from liver homogenate. Results: In MEHS treated groups (50 and 100 mg/kg b. w.) tumor volume, tumor weight, viable cell count was significantly decreased as compar...
This research was conducted to evaluate the possible antihelmintic activity of leaf and stem of C... more This research was conducted to evaluate the possible antihelmintic activity of leaf and stem of Carica papaya as a potent remedy in both human and veterinary practices using Indian adult earth worm (Pheretima posthuma) as a test worm. Various concentration (5%, 2.5%, 1%) were invitro tested using different extracts like ( hydroalcoholic, chloroform) and the results were involved in terms of time for paralysis (P) and time for death (D). It showed shortest time for paralysis in 5% concentration while the time for paralysis and death will increase in 2.5% and 1% respectively as compared to standard drug Albendazole. The present study indicated that the leaf and stem of Carica papaya has a significant anthelmintic activity and can be a potent drug due to, cost benefit and easy availability.
Journal of Planar Chromatography - Modern TLC, 2018
Asparagus racemosus has been gaining importance due to its recently detected pharmacological acti... more Asparagus racemosus has been gaining importance due to its recently detected pharmacological activities. There has been a need to develop a simple isolation method for the major bioactive phytochemical and its quantitative evaluation in the herb. Hence, a rapid, sensitive, and reproducible high-performance thin-layer chromatography (HPTLC) method has been developed for determining its major bioactive marker, shatavarin IV. HPTLC silica gel F254 pre-coated plates were used with ethyl acetate–methanol–water (7.5:1.5:1, v/v) as the mobile phase. The plates were developed to a distance of 60 mm at 23 ± 4°C in twin-trough chamber, previously saturated for 5 min. Under these conditions, the retention factor (RF) of shatavarin IV was 0.43 and it was quantified at 425 nm, the wavelength of maximum absorbance after derivatization in anisaldehyde–H2SO4 reagent. Shatavarin IV showed a linear function of amount 600 to 1800 ng per band; the correlation coefficient was 0.9934, indicating a good relationship between peak area and amount. The limits of detection (LOD) and quantitation (LOQ) were 14.35 ng and 43.50 ng, respectively. The method was validated for precision, repeatability, and accuracy. Recovery was determined by spiking samples with shatavarin IV before extraction and it was found to be 96.17%. Shatavarin IV was found to be present in roots (0.40%) and in enriched fraction (61.20%).
The present study assessed the methanol extract of Streblus asper stem bark (MESA) for antitumor ... more The present study assessed the methanol extract of Streblus asper stem bark (MESA) for antitumor effect and antioxidant influence against Ehrlich ascites carcinoma (EAC) in Swiss albino mice. Twenty four hours after intraperitonial inoculation of tumor (EAC) cells in mice, MESA was administered at 200 and 400 mg/kg body weight daily for 9 consecutive days. On the 10 th day, half of the mice were sacrificed for estimation of tumor parameters, haematological, liver and kidney antioxidant parameters; and the rest were kept alive for assessment of survival. MESA exhibited dose dependent and significant (p < 0.01) decrease in tumor proliferation and extended the life span of EAC bearing mice. Hematological profiles were significantly (p < 0.01) restored near to normal in MESA treated mice as compared to EAC control. MESA treatment significantly (p < 0.01) modulated the hepatic and renal antioxidant parameters as compared to EAC control. The present study demonstrated that S. asp...
Objective: Evaluation of anti cancer activity of methanol extracts of Curcuma zedoaria against Eh... more Objective: Evaluation of anti cancer activity of methanol extracts of Curcuma zedoaria against Ehrlich’s ascities carcinoma (EAC) cell line in Swiss albino mice. Method: In vitro cytotoxicity assay has been evaluated by using the trypan blue and MTT assay method. In vivo anti cancer activity was performed by using EAC cells induced mice groups (n=12), at the doses of 100 and 200 mg/kg b.w. respectively, half of the mice were sacrificed and the rest were kept alive for life span parameter. The anti cancer potential of MECZ was assessed by evaluating tumor volume, viable and nonviable tumor cell count, tumor weight, hematological parameters and biochemical estimations. Furthermore, antioxidant parameters were assayed by estimating liver tissue enzymes. Result: Dose dependent cytotoxicity was observed in (* p < 0.05) Trypan blue and MTT assay method. In vivo anti cancer parameters like tumor volume, tumor weight, and viable cell count were decreased compared to the EAC control group...
ABSTRACTS Objective: Anticancer activity of methanol extract of Hippophae salicifolia (MEHS) bark... more ABSTRACTS Objective: Anticancer activity of methanol extract of Hippophae salicifolia (MEHS) bark was evaluated using the Ehrlich Ascites Carcinoma (EAC) cells on Swiss albino mice. Methods: In vitro cytotoxicity assay has been evaluated by using the trypan blue and MTT assay method. The determination of in vivo anticancer activity was performed using EAC cells (2 × 106 Results: In MEHS treated groups (50 and 100 mg/kg b. w.) tumor volume, tumor weight, viable cell count was significantly decreased as compared to that of the EAC control group. Life span increased by 34% and 43% in a dose dependant manner as compared to EAC control group. The hematological, biochemical and liver tissue antioxidant parameter are significantly (p < 0.05) restored towards the normal level after treatment with MEHS. ) induced mice groups (n = 12). After treatment with MEHS at the doses of 50 and 100 mg/kg b. w. respectively for 9 days, half of the mice of each group were sacrificed and the rest were k...
JPC – Journal of Planar Chromatography – Modern TLC
Herbal medicine is gaining its popularity day by day and gradually streaming toward integration i... more Herbal medicine is gaining its popularity day by day and gradually streaming toward integration into the mainstream healthcare systems. Therefore, standardization of herbal formulations is essential in order to assess quality of the drugs. Two commonly used paradigms, compound and pattern-based approaches, were performed to ensure quality and hence product consistency. A rapid, sensitive, and reproducible high-performance thin-layer chromatography (HPTLC) method has been developed for one of the major bioactive markers, piperine. The plates were developed using the mobile phase with toluene–ethyl acetate–formic acid–methanol (3:3:0.8:0.2, v/v). The method was validated for limit of detection, limit of quantification, linearity, specificity, precession, and recovery. The results indicate that the bioactive compound was present in 2.64% w/w in the fruit of Piper nigrum Linn. and 0.253% in a herbal formulation. Each ingredient was analyzed vis-a-vis the herbal formulation and the reflectance spectrum of the matching component(s) to ensure the presence of each ingredient in the herbal formulation. The individual component adding up has also resulted similar fingerprint pattern as that of herbal formulation. Thus, the TLC method could be a rapid tool for the delineation of each individual ingredient in the finished products for authenticity, efficacy, and for batch-to-batch consistency.
The present study evaluates the antioxidant effect of methanol extract of Hippophae salicifolia (... more The present study evaluates the antioxidant effect of methanol extract of Hippophae salicifolia (MEHS) bark with special emphasis on its role on oxidative DNA damage in mouse peritoneal macrophages. In vitro antioxidant activity was estimated by standard antioxidant assays whereas the antioxidant activity concluded the H(+) donating capacity. Mouse erythrocytes&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39; hemolysis and peritoneal macrophages&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39; DNA damage were determined spectrophotometrically. In vivo antioxidant activity of MEHS was determined in carbon tetrachloride-induced mice by studying its effect on superoxide anion production in macrophages cells, superoxide dismutase in the cell lysate, DNA damage, lipid peroxidation, and reduces glutathione. The extract showed good in vitro antioxidant activities whereas the inhibitory concentrations values ranged from 5.80 to 106.5 μg/ml. MEHS significantly (P &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.05) attenuated the oxidative DNA damage. It also attenuated the oxidative conversion of hemoglobin to methemoglobin and elevation of enzymatic and nonenzymatic antioxidant in cells. The result indicates MEHS has good in vitro-in vivo antioxidant property as well as the protective effect on DNA and red blood cell may be due to its H(+) donating property.
Many of the major chemotherapeutic agents are secondary metabolites found in nature. Zanthoxylum ... more Many of the major chemotherapeutic agents are secondary metabolites found in nature. Zanthoxylum alatum Roxb. (Rutaceae) is traditionally used in the treatment of various diseases. The present study evaluates the apoptotic activity of methanol extract of Z. alatum (MEZA) on Ehrlich ascites tumor (EAT) in Swiss albino mice. The presence of flavonoids in MEZA was standardized by HPLC. The in vitro cytotoxicity of MEZA was measured by the MTT assay. The in vivo antitumor activity of MEZA (100 and 200 mg/kg b.w., i.p. for 9 days) was also evaluated. On the 10th day, EAT tumor volume, cell viability, and hematological parameters were assayed. Apoptotic morphology was determined by acredine orange/ethedium bromide using fluorescence microscopy. Apoptosis percentage was measured by flow cytometric analysis using annexine-V-FITC. Also, DNA damage and bcl-2/bax were estimated by UV-method and western blot, respectively. HPLC analysis revealed presence of three flavonoids, rutin, myricetin, a...
Many of the major chemotherapeutic agents are secondary metabolites found in nature. Zanthoxylum ... more Many of the major chemotherapeutic agents are secondary metabolites found in nature. Zanthoxylum alatum Roxb. (Rutaceae) is traditionally used in the treatment of various diseases. The present study evaluates the apoptotic activity of methanol extract of Z. alatum (MEZA) on Ehrlich ascites tumor (EAT) in Swiss albino mice. The presence of flavonoids in MEZA was standardized by HPLC. The in vitro cytotoxicity of MEZA was measured by the MTT assay. The in vivo antitumor activity of MEZA (100 and 200 mg/kg b.w., i.p. for 9 days) was also evaluated. On the 10th day, EAT tumor volume, cell viability, and hematological parameters were assayed. Apoptotic morphology was determined by acredine orange/ethedium bromide using fluorescence microscopy. Apoptosis percentage was measured by flow cytometric analysis using annexine-V-FITC. Also, DNA damage and bcl-2/bax were estimated by UV-method and western blot, respectively. HPLC analysis revealed presence of three flavonoids, rutin, myricetin, and quercetin. MEZA showed satisfactory cytotoxicity in MTT assay (IC50 = 111.50 µg/ml). The extract significantly (p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.01) changed the tumor volume, viable, non-viable cell count, and hematological parameters towards the normal. Apoptotic activity of MEZA was confirmed by acridine orange/ethidium bromide staining, annexin-V-FITC staining, DNA fragmentation, and Bcl-2/Bax ratio. The study showed that MEZA has antitumor activity which may be due to the presence of flavonoids in the extract.
Journal of experimental therapeutics & oncology, 2013
The present study assessed the methanol extract of Streblus asper stem bark (MESA) for antitumor ... more The present study assessed the methanol extract of Streblus asper stem bark (MESA) for antitumor effect and antioxidant influence against Ehrlich ascites carcinoma (EAC) in Swiss albino mice. Twenty four hours after intraperitonial inoculation of tumor (EAC) cells in mice, MESA was administered at 200 and 400 mg/kg body weight daily for 9 consecutive days. On the 10 th day, half of the mice were sacrificed for estimation of tumor parameters, haematological, liver and kidney antioxidant parameters; and the rest were kept alive for assessment of survival. MESA exhibited dose dependent and significant (p < 0.01) decrease in tumor proliferation and extended the life span of EAC bearing mice. Hematological profiles were significantly (p < 0.01) restored near to normal in MESA treated mice as compared to EAC control. MESA treatment significantly (p < 0.01) modulated the hepatic and renal antioxidant parameters as compared to EAC control. The present study demonstrated that S. asp...
Journal of Environmental Pathology, Toxicology and Oncology, 2015
Methanol extracts of Thevetia peruviana (METP) (Apocynaceae) fruit showed antitumor activity agai... more Methanol extracts of Thevetia peruviana (METP) (Apocynaceae) fruit showed antitumor activity against Ehrlich&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;s ascites carcinoma (EAC) cell line in Swiss albino mice. The METP-treated group&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;s tumor volume, tumor weight, and viable cell count were decreased compared to the EAC control group. Tumor volumes were 3.62±0.12 ml, 2.88±0.23 ml, and 1.34±0.17 ml for the EAC control group and the METP-treated groups (50 mg/kg and 100 mg/kg body weight), respectively. Nonviable cell counts were 4.44%±0.42%, 18.57%±3.07%, and 68.12%±5.32% in the EAC control group and the METP-treated groups (50 mg/kg and 100 mg/kg body weight), respectively. METP-treated EAC cell-bearing mice had an increased life span (48.69% and 83.78%) compared to the EAC control group. Hematological and serum biochemical profiles were restored to normal levels in METP-treated mice compared to the EAC control group. METP significantly (P&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;0.001) decreased lipid peroxidation and recovered reduced glutathione, superoxide dismutase, and catalase toward normal levels compared to the EAC control group. In summary, METP exhibited remarkable antitumor activity in Swiss albino mice, which is plausibly attributable to its augmentation of endogenous antioxidant mechanisms.
Journal of Environmental Pathology, Toxicology and Oncology, 2014
In the present study, we evaluated the ameliorative potential of a citrus flavonoid, naringenin (... more In the present study, we evaluated the ameliorative potential of a citrus flavonoid, naringenin (NRG), against experimentally induced arsenic toxicity in Swiss albino mice. NRG (5 and 10 mg kg-l) was administered orally to mice 30 minutes before oral administration of arsenic trioxide (3 mg kg-l) for 14 consecutive days. On day 15, the following parameters were evaluated: body weight; organ weight; hematological profile; serum biochemical profile; hepatic and renal tissue antioxidative parameters including lipid peroxidation, reduced and oxidized glutathione, glutathione-S-transferase, glutathione peroxidase, glutathione reductase, superoxide dismutase, catalase levels; and DNA fragmentation. Co-treatment with NRG markedly and significantly normalized body weights, organ weights, hematological profiles, and serum biochemical profiles and significantly modulated all of the hepatic and renal tissue biochemical parameters and DNA fragmentation in arsenic-intoxicated mice. The present findings indicate that naringenin remarkably ameliorated the effects of arsenic-induced toxicity in albino mice due to its strong antioxidant property.
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