International Journal of Applied Pharmaceutical Sciences and Research, 2016
A simple and validated reverse phase high performance liquid chromatography (RP-HPLC) method was ... more A simple and validated reverse phase high performance liquid chromatography (RP-HPLC) method was developed for simultaneous estimation of Esomeprazole and Domperidone. Different HPLC Chromatographic conditions were used to find out the optimum chromatographic condition for best elution of drugs. Good chromatographic separation was achieved on Develosil ODS HG-5 RP C18 (15cmX4.6mm, 5μm Particle size), analytical column using a mixture of Potassium dihydrogen phosphate buffer pH: acetonitrile in the ratio of 45:55 v/v used as mobile phase at the flow rate of 1 ml/min and detector wavelength at 284nm. The validation of the proposed method was carried out for specificity, linearity and range, accuracy, precision, limit of detection and limit of quantification. Linearity range was found to be 0-140 μgg/ml and 0-150 μgg/ml with correlation coefficients of 0.995 & 0.994 and retention time of 2.59 and 3.85 min for Domperidone and Esomeprazole respectively. The LOD was found to be 0.341 μg/m...
Abstract: The present study was aimed to evaluate the phytochemical, antidiabetic, and cytoprotec... more Abstract: The present study was aimed to evaluate the phytochemical, antidiabetic, and cytoprotective properties of Ber-beris aristata DC. (Berberidaceae) root extracts. After phytochemical evaluation of various extracts of B. aristata roots, the ethanol extract was found to be rich in phytochemicals with the presence of alkaloid determined by the thin layer chromatography; hence ethanol extract was selected for further study. Administration of ethanol extract of B. aristata roots in diabetic rats showed dose dependent reduction in hyperglycemia. The levels of serum total cholesterol, triglyc-eride, AST (aspartate aminotransferase), ALT (alanine aminotransferase), serum creatinine and blood urea were signifi-cantly decreased in diabetic rats when compared with diabetic control rats.
Background Cineraria maritima has a long history of use in the treatment of cataract and other ey... more Background Cineraria maritima has a long history of use in the treatment of cataract and other eye-related problems in the homeopathic system of medicines. High oxidative stress is one of the major underlying causes of cataract which results in the precipitation of natural protein present in the lenses with aging. This research has been carried out to determine the anti-cataract activity of C. maritima by performing various antioxidant techniques such as 1,1-diphenyl-2-picrylhydrazyl, nitric oxide, hydrogen peroxide, and studies in oxidative stress–induced ex vivo cataract model. Results Results of the study conducted in the ethanolic extract of aerial parts (leaves and stems) of C. maritima revealed the presence of various phytoconstituents such as alkaloids, phenols, flavonoids, etc. Total phenol and total flavonoid content was found to be 6.31 ± 0.06 % w/w and 2.14 ± 0.09% w/w respectively, which revealed that the plant contains a good amount of these compounds and hence possesse...
Green tea polyphenols have demonstrated significant antioxidant, anti-carcinogenic, anti-mutageni... more Green tea polyphenols have demonstrated significant antioxidant, anti-carcinogenic, anti-mutagenic and antidiabetic activity in numerous human, animal and in vitro studies. Hence present study was designed to evaluate the influence of green tea in ischemia induced memory loss in mice. Morris water maze, Elevated plus maze and passive avoidance apparatus was used for the evaluation of learning and memory. Brain thiobarbituric acid reactive substance was also estimated. Brain ischemia reduces the learning and memory performance and also increase the concentration of thiobarbituric acid reactive substance in mice. Green tea significantly improved the learning and memory and reversed the increased thiobarbituric acid reactive substance concentration in mice. The result of present study indicates that green tea improve the learning and memory. It also reduces the ischemia induced oxidative stress.
International Journal of Applied Pharmaceutics, 2018
Objective: The present study has focused on development and optimization of thermosensitive in-si... more Objective: The present study has focused on development and optimization of thermosensitive in-situ ocular drug delivery system for the treatment of conjunctivitis. Methods: Thermosensitive in-situ hydrogel formulation of moxifloxacin hydrochloride was developed by dispensing variable concentration of pluronic F-127 , gellan-gum, and carbopol in distilled water. Viscosity, gelation temperature and mean release time (MRT) were measured by using ‘Brookfield’ viscometer LV-III (spindle no. 40), rheological techniques and dissolution apparatus respectively. Optimization for ideal formulation was carried by ‘Box–Behnken’ design on the basis of prime factors of the formulation including viscosity, gelation temperature, and MRT. Moreover, the optimized formulation was evaluated for accelerated stability study by in vitro drug release, anti-microbial potential by ‘Kirby-Bauer disk diffusion’ method and ocular irritancy assay were done by in vivo analysis. Results: Optimised thermosensitive ...
The hepatoprotective activity of methanolic rhizome extract of Curculigo orchioides (MECO) were e... more The hepatoprotective activity of methanolic rhizome extract of Curculigo orchioides (MECO) were evaluated against Thioacetamide-induced hepatic damage in rats. The MECO at dose of 100, 200 and 400 mg/kg were administered orally once daily for 21 days and simultaneously administered TAA 100 mg/kg b.w. s.c. 1 h after the respective assigned treatments every 72 h. Serum enzymatic levels of serum glutamate oxaloacetate transaminase (AST), serum glutamate pyruvate transaminase (ALT), serum alkaline phosphatase (ALP) and total bilirubin were estimated along with estimation of superoxide dismutase (SOD) and malondialdehyde (MDA) levels in liver tissues.
Oxidative damage and antioxidants are assuming great interest in today’s modern world. Cancer, ca... more Oxidative damage and antioxidants are assuming great interest in today’s modern world. Cancer, cardiovascular diseases and other types of diseases may be due to the result of free radicals.1 With newer insights into the fact that oxidative species can lead to a number of health problems and complications, finding treatment of oxidative damage and oxidative stress is acquiring greater significance. Phytochemicals obtained from plants are gaining popularity day by day in countering harmful effects of oxidative species and diseases associated with oxidative damage. Various fruits, vegetables and medicinal plants contain numerous phytochemicals.1,2 Many phytochemicals have been isolated and tested for their therapeutic potentials but many phytochemicals are still there which need to be discovered in future. Phytochemicals have a wide variety of beneficial effects. They possess antioxidant activity, enhance immunity, reduce aggregation of platelets, influence the activity of hormones and...
In a wide search program towards new and efficient antimicrobial agents, substituted chalcones ha... more In a wide search program towards new and efficient antimicrobial agents, substituted chalcones have been synthesized by condensing benzaldehyde derivatives with acetophenone derivatives in dilute ethanolic sodium hydroxide solution at room temperature according to Claisen – Schmidt condensation. The structures of these compounds have be en investigated by infra red spectroscopy, nuclear magnetic resonance spectroscopy and mass spectrometry. The antimicrobial activity of the novel products was evaluated by Filter Paper Disc diffusion Method. The compound 1b showed excellent activity against S. aureus at both concentration i.e. 500μg/ml and1000 μg/ml.
The efficacy of seed extracts of Amomum subulatum Roxb. activity against bacterias such Staphyloc... more The efficacy of seed extracts of Amomum subulatum Roxb. activity against bacterias such Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus subtilis, Escheria Coli. were studied. The minimum inhibitory concentration (MIC) of the extracts was determined using standard methods. Results obtained showed considerable inhibition against the bacteria tested showed considerable res istance at all concentrations of the extract. Howev er the standard antibacterial drug Erythromycin exhibit superior activity than the extract . This activity was indicative of the possible means of finding pure active principles fr om natural source with possible high potency that c ould serve as a lead to the pharmaceuticals. The low concentration (MIC) activity of the extracts gives credence and s cientific base for the claim therapeutic capabilities of Amomum subulatum as an anti-bacterial agent. The oil extract yield of the seed extracts was estimated to be 1.2 mL which is p ale yellow in colour. The stu...
Journal of Pharmaceutical Research International, 2021
Objective: Raloxifene HCl (RLX) show a low oral bioavailability (<2%) in human due to its poor... more Objective: Raloxifene HCl (RLX) show a low oral bioavailability (<2%) in human due to its poor solubility and extensive (>90%) first-pass metabolism. To overcome these limitations, the present investigation deals with the development of Compritol 888 ATO based optimized RLX-loaded solid lipid nanoparticle (SLN) . Methods: Compritol 888 ATO-based RLX-loaded optimized solid lipid nanoparticle (SLN) were formulated using oil in water microemulsion method. Drug-excipients compatibility was confirmed through a powder X-ray diffraction study. The optimized SLN was characterized for particle size and drug release. Results: Drug-excipients compatibility was confirmed powder X-ray diffraction study. As per the desirability function value (0.959) the optimized formulation has the size and EE of 168.97 nm and 93.14%. In-vitro study showed a sustained release of RLX from the optimized SLNs. The optimized formulation was found stable for 180 days. Conclusion: The high biocompatibility, bio...
Medicinal plants find use across different cultures in the world. People have derived numerous be... more Medicinal plants find use across different cultures in the world. People have derived numerous benefits by using various plants for centuries. Herbal drugs are supposed to be safe, but there are chances these might cause toxicity and other complications. Thus toxicity studies are essential for a medicinal plant before being used in humans. The present research revolves around studying the acute toxicity of Ocimum kilimandscharicum, Thymus serpyllum, Spilanthes acmella and their combination in equal ratio (COMB). For preparing the combination of extracts, the ethanolic extracts of the three plants were combined in equal amounts and administered to the animals. The acute toxicity study was carried out according to OECD (The Organisation for Economic Co-operation and Development) guideline 423 for acute toxicity. The animals were divided into four groups each containing three animals each. These groups were respectively administered ethanolic extract of O. kilimandscharicum, T. serpyll...
Aims: To understand about the nanoemulsion types and the process formation of spontaneous emulsif... more Aims: To understand about the nanoemulsion types and the process formation of spontaneous emulsification method by phase inversion. Then to test the different combinations of Oil, Surfactants and Co-surfactants for formation of suitable nanoemulsions for phenytoin drug loading. Study Design: Spontaneous emulsification method by phase inversion used to form the nanoemulsions. Place and Duration of Study: Department of Pharmaceutical Sciences, Kumaun University, Nainital, Uttarakhand, India. Methodology: Phenytoin is a widely used drug in anticonvulsants class for epilepsy which comes under BCS Class II of drug category. Phenytoin has high permeability property but it also shows low solubility property which makes it difficult to absorb from GI tract hence make a poor penetration into the brain to target disease in the CNS. To overcome the situation of poor delivery of phenytoin, the requirement of nanoparticulate drug delivery as an innovative and effective drug delivery system from ...
Summary The objective of the study is to find out the current status of the ‘prescribing practice... more Summary The objective of the study is to find out the current status of the ‘prescribing practices’ in the government hospitals of Almora located in India. The survey was conducted in three hospitals of Almora. Consecutive random sampling method was applied for collection of prescriptions from the health facilities. Overall the average number of drugs prescribed per prescription was very high which indicates a trend towards polypharmacy. The most commonly prescribed drugs were NSAIDs, antimicrobials and antiulcers. Drugs prescribed by brand name were 75%. The study showed that the prescribing practices of the Almora is more of an irrational types like polypharmacy, overuse of antibiotics, less number in generic name, so there is an urgent need for some interventions to improve the situation.
International Journal of Pharmaceutical Sciences and Nanotechnology, 1970
For improving bioavailability in controlled release fashion and to circumvent the hepatic first p... more For improving bioavailability in controlled release fashion and to circumvent the hepatic first pass effect of glipizide mucoadhesive buccal films of glipizide were prepared by solvent casting technique. Buccal films were prepared using hydroxy propylmethylcellulose, sodium carboxymethylcellulose, carbopol-934P and Eudragit RL-100. Films were evaluated for their weight, thickness, surface pH, swelling index, in vitro residence time, folding endurance, in vitro release, ex vivo permeation studies and drug content uniformity. The films exhibited controlled release over more than 6 h. From the study it was concluded that the films containing 5 mg glipizide in 4.9 % w/v hydroxy propylmethylcellulose and 1.5 % w/v sodium carboxymethylcellulose exhibited satisfactory swelling, an optimum residence time and promising drug release thus proved to be potential candidate for the development of buccal films for therapeutic use.
Centratherum anthelminticum Asteraceae (Compositae) is a (Wild) Kuntze has been obtained from the... more Centratherum anthelminticum Asteraceae (Compositae) is a (Wild) Kuntze has been obtained from the north of India Uttarakhand state. The antibacterial and antifungal effects of chloroform of plant seeds were tested against the different bacteria and fungus eg. Stalophylococous aureous ATCC-29737, Escherischia coli ATCC-14169, Pseudomonas aerugenosa ATCC-9027, Bacillus Subtilis ATCC-6633 Fungus Colletotrichum gloeosporioides, Phomopsis dalbergiae, Trichoderma piluliferum. By disc diffusion method or microdilution technique in-vitro. The growth of E-coli, Pseudomonas aerugenosa the gram-negative bacteria and Fungus, have been inhibited by the chloroform extracts of the seeds of the Centratherum anthelminticum the extracts did not prevent the growth of the other test organism. This improves the existence of the antimicrobial and antifungal activity of the plant. The results showed that the seeds extract of Centratherum anthelminticum had the strong antibacterial activity of 0.0020 µg/ml...
International Journal of Applied Pharmaceutical Sciences and Research, 2016
A simple and validated reverse phase high performance liquid chromatography (RP-HPLC) method was ... more A simple and validated reverse phase high performance liquid chromatography (RP-HPLC) method was developed for simultaneous estimation of Esomeprazole and Domperidone. Different HPLC Chromatographic conditions were used to find out the optimum chromatographic condition for best elution of drugs. Good chromatographic separation was achieved on Develosil ODS HG-5 RP C18 (15cmX4.6mm, 5μm Particle size), analytical column using a mixture of Potassium dihydrogen phosphate buffer pH: acetonitrile in the ratio of 45:55 v/v used as mobile phase at the flow rate of 1 ml/min and detector wavelength at 284nm. The validation of the proposed method was carried out for specificity, linearity and range, accuracy, precision, limit of detection and limit of quantification. Linearity range was found to be 0-140 μgg/ml and 0-150 μgg/ml with correlation coefficients of 0.995 & 0.994 and retention time of 2.59 and 3.85 min for Domperidone and Esomeprazole respectively. The LOD was found to be 0.341 μg/m...
Abstract: The present study was aimed to evaluate the phytochemical, antidiabetic, and cytoprotec... more Abstract: The present study was aimed to evaluate the phytochemical, antidiabetic, and cytoprotective properties of Ber-beris aristata DC. (Berberidaceae) root extracts. After phytochemical evaluation of various extracts of B. aristata roots, the ethanol extract was found to be rich in phytochemicals with the presence of alkaloid determined by the thin layer chromatography; hence ethanol extract was selected for further study. Administration of ethanol extract of B. aristata roots in diabetic rats showed dose dependent reduction in hyperglycemia. The levels of serum total cholesterol, triglyc-eride, AST (aspartate aminotransferase), ALT (alanine aminotransferase), serum creatinine and blood urea were signifi-cantly decreased in diabetic rats when compared with diabetic control rats.
Background Cineraria maritima has a long history of use in the treatment of cataract and other ey... more Background Cineraria maritima has a long history of use in the treatment of cataract and other eye-related problems in the homeopathic system of medicines. High oxidative stress is one of the major underlying causes of cataract which results in the precipitation of natural protein present in the lenses with aging. This research has been carried out to determine the anti-cataract activity of C. maritima by performing various antioxidant techniques such as 1,1-diphenyl-2-picrylhydrazyl, nitric oxide, hydrogen peroxide, and studies in oxidative stress–induced ex vivo cataract model. Results Results of the study conducted in the ethanolic extract of aerial parts (leaves and stems) of C. maritima revealed the presence of various phytoconstituents such as alkaloids, phenols, flavonoids, etc. Total phenol and total flavonoid content was found to be 6.31 ± 0.06 % w/w and 2.14 ± 0.09% w/w respectively, which revealed that the plant contains a good amount of these compounds and hence possesse...
Green tea polyphenols have demonstrated significant antioxidant, anti-carcinogenic, anti-mutageni... more Green tea polyphenols have demonstrated significant antioxidant, anti-carcinogenic, anti-mutagenic and antidiabetic activity in numerous human, animal and in vitro studies. Hence present study was designed to evaluate the influence of green tea in ischemia induced memory loss in mice. Morris water maze, Elevated plus maze and passive avoidance apparatus was used for the evaluation of learning and memory. Brain thiobarbituric acid reactive substance was also estimated. Brain ischemia reduces the learning and memory performance and also increase the concentration of thiobarbituric acid reactive substance in mice. Green tea significantly improved the learning and memory and reversed the increased thiobarbituric acid reactive substance concentration in mice. The result of present study indicates that green tea improve the learning and memory. It also reduces the ischemia induced oxidative stress.
International Journal of Applied Pharmaceutics, 2018
Objective: The present study has focused on development and optimization of thermosensitive in-si... more Objective: The present study has focused on development and optimization of thermosensitive in-situ ocular drug delivery system for the treatment of conjunctivitis. Methods: Thermosensitive in-situ hydrogel formulation of moxifloxacin hydrochloride was developed by dispensing variable concentration of pluronic F-127 , gellan-gum, and carbopol in distilled water. Viscosity, gelation temperature and mean release time (MRT) were measured by using ‘Brookfield’ viscometer LV-III (spindle no. 40), rheological techniques and dissolution apparatus respectively. Optimization for ideal formulation was carried by ‘Box–Behnken’ design on the basis of prime factors of the formulation including viscosity, gelation temperature, and MRT. Moreover, the optimized formulation was evaluated for accelerated stability study by in vitro drug release, anti-microbial potential by ‘Kirby-Bauer disk diffusion’ method and ocular irritancy assay were done by in vivo analysis. Results: Optimised thermosensitive ...
The hepatoprotective activity of methanolic rhizome extract of Curculigo orchioides (MECO) were e... more The hepatoprotective activity of methanolic rhizome extract of Curculigo orchioides (MECO) were evaluated against Thioacetamide-induced hepatic damage in rats. The MECO at dose of 100, 200 and 400 mg/kg were administered orally once daily for 21 days and simultaneously administered TAA 100 mg/kg b.w. s.c. 1 h after the respective assigned treatments every 72 h. Serum enzymatic levels of serum glutamate oxaloacetate transaminase (AST), serum glutamate pyruvate transaminase (ALT), serum alkaline phosphatase (ALP) and total bilirubin were estimated along with estimation of superoxide dismutase (SOD) and malondialdehyde (MDA) levels in liver tissues.
Oxidative damage and antioxidants are assuming great interest in today’s modern world. Cancer, ca... more Oxidative damage and antioxidants are assuming great interest in today’s modern world. Cancer, cardiovascular diseases and other types of diseases may be due to the result of free radicals.1 With newer insights into the fact that oxidative species can lead to a number of health problems and complications, finding treatment of oxidative damage and oxidative stress is acquiring greater significance. Phytochemicals obtained from plants are gaining popularity day by day in countering harmful effects of oxidative species and diseases associated with oxidative damage. Various fruits, vegetables and medicinal plants contain numerous phytochemicals.1,2 Many phytochemicals have been isolated and tested for their therapeutic potentials but many phytochemicals are still there which need to be discovered in future. Phytochemicals have a wide variety of beneficial effects. They possess antioxidant activity, enhance immunity, reduce aggregation of platelets, influence the activity of hormones and...
In a wide search program towards new and efficient antimicrobial agents, substituted chalcones ha... more In a wide search program towards new and efficient antimicrobial agents, substituted chalcones have been synthesized by condensing benzaldehyde derivatives with acetophenone derivatives in dilute ethanolic sodium hydroxide solution at room temperature according to Claisen – Schmidt condensation. The structures of these compounds have be en investigated by infra red spectroscopy, nuclear magnetic resonance spectroscopy and mass spectrometry. The antimicrobial activity of the novel products was evaluated by Filter Paper Disc diffusion Method. The compound 1b showed excellent activity against S. aureus at both concentration i.e. 500μg/ml and1000 μg/ml.
The efficacy of seed extracts of Amomum subulatum Roxb. activity against bacterias such Staphyloc... more The efficacy of seed extracts of Amomum subulatum Roxb. activity against bacterias such Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus subtilis, Escheria Coli. were studied. The minimum inhibitory concentration (MIC) of the extracts was determined using standard methods. Results obtained showed considerable inhibition against the bacteria tested showed considerable res istance at all concentrations of the extract. Howev er the standard antibacterial drug Erythromycin exhibit superior activity than the extract . This activity was indicative of the possible means of finding pure active principles fr om natural source with possible high potency that c ould serve as a lead to the pharmaceuticals. The low concentration (MIC) activity of the extracts gives credence and s cientific base for the claim therapeutic capabilities of Amomum subulatum as an anti-bacterial agent. The oil extract yield of the seed extracts was estimated to be 1.2 mL which is p ale yellow in colour. The stu...
Journal of Pharmaceutical Research International, 2021
Objective: Raloxifene HCl (RLX) show a low oral bioavailability (<2%) in human due to its poor... more Objective: Raloxifene HCl (RLX) show a low oral bioavailability (<2%) in human due to its poor solubility and extensive (>90%) first-pass metabolism. To overcome these limitations, the present investigation deals with the development of Compritol 888 ATO based optimized RLX-loaded solid lipid nanoparticle (SLN) . Methods: Compritol 888 ATO-based RLX-loaded optimized solid lipid nanoparticle (SLN) were formulated using oil in water microemulsion method. Drug-excipients compatibility was confirmed through a powder X-ray diffraction study. The optimized SLN was characterized for particle size and drug release. Results: Drug-excipients compatibility was confirmed powder X-ray diffraction study. As per the desirability function value (0.959) the optimized formulation has the size and EE of 168.97 nm and 93.14%. In-vitro study showed a sustained release of RLX from the optimized SLNs. The optimized formulation was found stable for 180 days. Conclusion: The high biocompatibility, bio...
Medicinal plants find use across different cultures in the world. People have derived numerous be... more Medicinal plants find use across different cultures in the world. People have derived numerous benefits by using various plants for centuries. Herbal drugs are supposed to be safe, but there are chances these might cause toxicity and other complications. Thus toxicity studies are essential for a medicinal plant before being used in humans. The present research revolves around studying the acute toxicity of Ocimum kilimandscharicum, Thymus serpyllum, Spilanthes acmella and their combination in equal ratio (COMB). For preparing the combination of extracts, the ethanolic extracts of the three plants were combined in equal amounts and administered to the animals. The acute toxicity study was carried out according to OECD (The Organisation for Economic Co-operation and Development) guideline 423 for acute toxicity. The animals were divided into four groups each containing three animals each. These groups were respectively administered ethanolic extract of O. kilimandscharicum, T. serpyll...
Aims: To understand about the nanoemulsion types and the process formation of spontaneous emulsif... more Aims: To understand about the nanoemulsion types and the process formation of spontaneous emulsification method by phase inversion. Then to test the different combinations of Oil, Surfactants and Co-surfactants for formation of suitable nanoemulsions for phenytoin drug loading. Study Design: Spontaneous emulsification method by phase inversion used to form the nanoemulsions. Place and Duration of Study: Department of Pharmaceutical Sciences, Kumaun University, Nainital, Uttarakhand, India. Methodology: Phenytoin is a widely used drug in anticonvulsants class for epilepsy which comes under BCS Class II of drug category. Phenytoin has high permeability property but it also shows low solubility property which makes it difficult to absorb from GI tract hence make a poor penetration into the brain to target disease in the CNS. To overcome the situation of poor delivery of phenytoin, the requirement of nanoparticulate drug delivery as an innovative and effective drug delivery system from ...
Summary The objective of the study is to find out the current status of the ‘prescribing practice... more Summary The objective of the study is to find out the current status of the ‘prescribing practices’ in the government hospitals of Almora located in India. The survey was conducted in three hospitals of Almora. Consecutive random sampling method was applied for collection of prescriptions from the health facilities. Overall the average number of drugs prescribed per prescription was very high which indicates a trend towards polypharmacy. The most commonly prescribed drugs were NSAIDs, antimicrobials and antiulcers. Drugs prescribed by brand name were 75%. The study showed that the prescribing practices of the Almora is more of an irrational types like polypharmacy, overuse of antibiotics, less number in generic name, so there is an urgent need for some interventions to improve the situation.
International Journal of Pharmaceutical Sciences and Nanotechnology, 1970
For improving bioavailability in controlled release fashion and to circumvent the hepatic first p... more For improving bioavailability in controlled release fashion and to circumvent the hepatic first pass effect of glipizide mucoadhesive buccal films of glipizide were prepared by solvent casting technique. Buccal films were prepared using hydroxy propylmethylcellulose, sodium carboxymethylcellulose, carbopol-934P and Eudragit RL-100. Films were evaluated for their weight, thickness, surface pH, swelling index, in vitro residence time, folding endurance, in vitro release, ex vivo permeation studies and drug content uniformity. The films exhibited controlled release over more than 6 h. From the study it was concluded that the films containing 5 mg glipizide in 4.9 % w/v hydroxy propylmethylcellulose and 1.5 % w/v sodium carboxymethylcellulose exhibited satisfactory swelling, an optimum residence time and promising drug release thus proved to be potential candidate for the development of buccal films for therapeutic use.
Centratherum anthelminticum Asteraceae (Compositae) is a (Wild) Kuntze has been obtained from the... more Centratherum anthelminticum Asteraceae (Compositae) is a (Wild) Kuntze has been obtained from the north of India Uttarakhand state. The antibacterial and antifungal effects of chloroform of plant seeds were tested against the different bacteria and fungus eg. Stalophylococous aureous ATCC-29737, Escherischia coli ATCC-14169, Pseudomonas aerugenosa ATCC-9027, Bacillus Subtilis ATCC-6633 Fungus Colletotrichum gloeosporioides, Phomopsis dalbergiae, Trichoderma piluliferum. By disc diffusion method or microdilution technique in-vitro. The growth of E-coli, Pseudomonas aerugenosa the gram-negative bacteria and Fungus, have been inhibited by the chloroform extracts of the seeds of the Centratherum anthelminticum the extracts did not prevent the growth of the other test organism. This improves the existence of the antimicrobial and antifungal activity of the plant. The results showed that the seeds extract of Centratherum anthelminticum had the strong antibacterial activity of 0.0020 µg/ml...
Uploads
Papers by vijay juyal