i am doing research in aquatic biotechnology and genetics mainly fish genetics - giving lectures to undergraduates of Faculty of Aquatic and Fisheries Sciences in: aquatic biology, marine biotechnology, aquatic animals genetics ans molecular cell biology.- supervising M.Sc. theses in biotechnology.
Background: Bromuconazole is a widely used triazole pesticide which is toxic not only to target f... more Background: Bromuconazole is a widely used triazole pesticide which is toxic not only to target fungi but also to animals and human. Aim: The present study aimed to evaluate the harmful effects of prolonged administration of bromuconazole fungicide-induced testicular toxicity in male rats. Methods: Rats were randomly allocated into 5 groups (10 rats per group) as following: group 1 (G1), the control group were given distilled water; G2 and G3 were orally administrated bromuconazole [at doses of 1/10 the oral LD50 = 32.8 mg /kg body weight (bw) and lowest relevant oral dose of no observed adverse effect level (NOAEL) = 13.8 mg /kg bw, respectively] by stomach gavage; and G4 and G5 were topically treated with bromuconazole (at doses of 1/10 dermal LD50 = 200 mg /kg bw and dermal NOAEL = 84 mg/kg bw, respectively). Results: Among the four doses only high oral dose (G2) showed a deteriorated blood picture. However, the four treated groups (G2-G5) showed a significant reduction in total ...
The need for identification and detection of genetically modified (GM) products is a must. Labeli... more The need for identification and detection of genetically modified (GM) products is a must. Labeling and traceability of GM material are important to address the concerns of both the consumers and the regulator authority. In the present study, a reliable technique was developed to detect the presence of GM Salmon (Salmo salar) and Tilapia (Oreochromis niloticus) carrying a transgenic growth hormone (GH) gene construct. The results revealed the absence of GM products in all tested samples (25 samples from each product type). The developed screening method in this research will permit greater coverage of the possible GM ingredient in fish products in the Egyptian markets.
Background: Doxorubicin (DOX) is a highly effective chemotherapeutic agent which use has been res... more Background: Doxorubicin (DOX) is a highly effective chemotherapeutic agent which use has been restricted due to its multi-organ toxicity. Objective: We investigated the possible protective effect of Calligonum comosum extract (CCE) against DOX toxicity while identifying its major phytoconstituents. Materials and Methods: CCE was administered at 100 mg/kg b.w for 2 weeks to rats which have previously received an intraperitoneal injection of DOX (20 mg/kg). Major phytoconstituents of CCE were assessed using high-performance liquid chromatography and UPLC/MS/MS. Results: CCE was rich in phenolic constituents, especially proanthocyanidins, corresponding to a concentration of 167 mg catechin per gram of the extract after hydrolysis. Other constituents identified were procyanidin B1-gallate, procyanidin B2-gallate, quercetin, and kaempferol. Animals that received CCE following DOX injection showed less signs of oxidative stress (indicated by levels of malondialdehyde, superoxide dismutase...
Ammonia is a critical hazardous nitrogen metabolic product in aquaculture. Despite trials for its... more Ammonia is a critical hazardous nitrogen metabolic product in aquaculture. Despite trials for its control, ammonia intoxication remains one of the most critical issues to overcome. In this study, we explored the modulatory effect and potential mechanism by which Yucca schidigera extract (YSE) can ameliorate ammonia intoxication-induced adverse effects on tilapia health and metabolism. A total number of 120 Nile tilapia were evenly assigned into four groups with three replicates each. The first group served as normal control group; the second group was exposed to ammonia alone from the beginning of the experiment and for four weeks. The third group was supplied with YSE in water at a dose of 8 mg/L and exposed to ammonia. The fourth group was supplied with YSE only in water at a dose of 8 mg/L. YSE supplementation succeeded in improving water quality by reducing pH and ammonia levels. Moreover, YSE supplementation markedly alleviated chronic ammonia-induced adverse impacts on fish gr...
This study was conducted to investigate the effect of dietary supplementation of Aspergillus awam... more This study was conducted to investigate the effect of dietary supplementation of Aspergillus awamori on aflatoxin B1 (AFB1)-induced liver damage in rabbits. Administration of AFB1 (0.3 mg/kg diet) led to a significant reduction in body weight, body weight gain, total feed intake, total serum proteins, albumin, high density lipoprotein-cholesterol, phagocytic activity, phagocytic index, and the antioxidant enzyme, glutathione peroxidase (GPx). Moreover, AFB1 administration was associated with a significant increase in feed conversion ratio, lipid peroxidation and serum levels of aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, gamma-glutamyl transferase, lactate dehydrogenase and total bilirubin. In addition, livers of AFB1-supplemented animals showed fatty degeneration with vacuolisation, focal areas of necrosis, mononuclear cells infiltration hyperplasia of bile ducts and sinusoids. A significant increase in the hepatic expression of the biotransformation...
Hepatocellular carcinoma (HCC) is the most common cancer in humans. Despite advances in its treat... more Hepatocellular carcinoma (HCC) is the most common cancer in humans. Despite advances in its treatment, liver cancer remains one of the most difficult cancers to treat. This study aimed to investigate the ameliorative action and potential mechanism of Aspergillus awamori (ASP) administration against the initiation process of liver carcinogenesis induced by diethylnitrosamine (DEN) in male Wistar rats. Seventy-two male rats were divided equally into eight groups as follows, Group 1: untreated control; Group 2: DEN (200 mg/kg bw) intra-peritoneally for the initiation of HCC; Groups 3–5: DEN + ASP at a dose of 1, 0.5, and 0.25 mg/kg bw and groups 6–8: ASP at a dose of 1, 0.5, and 0.25 mg/kg bw. Supplementation of A. awamori significantly lightened the adverse impacts induced by DEN via restoring the leukogram to normal, lowering the elevated serum aspartate aminotransferase (AST), alanine transaminase (ALT), and γ-glutamyl transferase (GGT), and alkaline phosphatase (ALP). Furthermore, ...
Background Collagen is the most abundant structural protein in the mammalian connective tissue an... more Background Collagen is the most abundant structural protein in the mammalian connective tissue and represents approximately 30% of animal protein. The current study evaluated the potential capacity of collagen extract derived from Nile tilapia skin in improving the cutaneous wound healing in rats and investigated the underlying possible mechanisms. A rat model was used, and the experimental design included a control group (CG) and the tilapia collagen treated group (TCG). Full-thickness wounds were conducted on the back of all the rats under general anesthesia, then the tilapia collagen extract was applied topically on the wound area of TCG. Wound areas of the two experimental groups were measured on days 0, 3, 6, 9, 12, and 15 post-wounding. The stages of the wound granulation tissues were detected by histopathologic examination and the expression of vascular endothelial growth factor (VEGF), and transforming growth factor (TGF-ß1) were investigated using immunohistochemistry. More...
Background: Bromuconazole is a widely used triazole pesticide which is toxic not only to target f... more Background: Bromuconazole is a widely used triazole pesticide which is toxic not only to target fungi but also to animals and human. Aim: The present study aimed to evaluate the harmful effects of prolonged administration of bromuconazole fungicide-induced testicular toxicity in male rats. Methods: Rats were randomly allocated into 5 groups (10 rats per group) as following: group 1 (G1), the control group were given distilled water; G2 and G3 were orally administrated bromuconazole [at doses of 1/10 the oral LD50 = 32.8 mg /kg body weight (bw) and lowest relevant oral dose of no observed adverse effect level (NOAEL) = 13.8 mg /kg bw, respectively] by stomach gavage; and G4 and G5 were topically treated with bromuconazole (at doses of 1/10 dermal LD50 = 200 mg /kg bw and dermal NOAEL = 84 mg/kg bw, respectively). Results: Among the four doses only high oral dose (G2) showed a deteriorated blood picture. However, the four treated groups (G2-G5) showed a significant reduction in total ...
The need for identification and detection of genetically modified (GM) products is a must. Labeli... more The need for identification and detection of genetically modified (GM) products is a must. Labeling and traceability of GM material are important to address the concerns of both the consumers and the regulator authority. In the present study, a reliable technique was developed to detect the presence of GM Salmon (Salmo salar) and Tilapia (Oreochromis niloticus) carrying a transgenic growth hormone (GH) gene construct. The results revealed the absence of GM products in all tested samples (25 samples from each product type). The developed screening method in this research will permit greater coverage of the possible GM ingredient in fish products in the Egyptian markets.
Background: Doxorubicin (DOX) is a highly effective chemotherapeutic agent which use has been res... more Background: Doxorubicin (DOX) is a highly effective chemotherapeutic agent which use has been restricted due to its multi-organ toxicity. Objective: We investigated the possible protective effect of Calligonum comosum extract (CCE) against DOX toxicity while identifying its major phytoconstituents. Materials and Methods: CCE was administered at 100 mg/kg b.w for 2 weeks to rats which have previously received an intraperitoneal injection of DOX (20 mg/kg). Major phytoconstituents of CCE were assessed using high-performance liquid chromatography and UPLC/MS/MS. Results: CCE was rich in phenolic constituents, especially proanthocyanidins, corresponding to a concentration of 167 mg catechin per gram of the extract after hydrolysis. Other constituents identified were procyanidin B1-gallate, procyanidin B2-gallate, quercetin, and kaempferol. Animals that received CCE following DOX injection showed less signs of oxidative stress (indicated by levels of malondialdehyde, superoxide dismutase...
Ammonia is a critical hazardous nitrogen metabolic product in aquaculture. Despite trials for its... more Ammonia is a critical hazardous nitrogen metabolic product in aquaculture. Despite trials for its control, ammonia intoxication remains one of the most critical issues to overcome. In this study, we explored the modulatory effect and potential mechanism by which Yucca schidigera extract (YSE) can ameliorate ammonia intoxication-induced adverse effects on tilapia health and metabolism. A total number of 120 Nile tilapia were evenly assigned into four groups with three replicates each. The first group served as normal control group; the second group was exposed to ammonia alone from the beginning of the experiment and for four weeks. The third group was supplied with YSE in water at a dose of 8 mg/L and exposed to ammonia. The fourth group was supplied with YSE only in water at a dose of 8 mg/L. YSE supplementation succeeded in improving water quality by reducing pH and ammonia levels. Moreover, YSE supplementation markedly alleviated chronic ammonia-induced adverse impacts on fish gr...
This study was conducted to investigate the effect of dietary supplementation of Aspergillus awam... more This study was conducted to investigate the effect of dietary supplementation of Aspergillus awamori on aflatoxin B1 (AFB1)-induced liver damage in rabbits. Administration of AFB1 (0.3 mg/kg diet) led to a significant reduction in body weight, body weight gain, total feed intake, total serum proteins, albumin, high density lipoprotein-cholesterol, phagocytic activity, phagocytic index, and the antioxidant enzyme, glutathione peroxidase (GPx). Moreover, AFB1 administration was associated with a significant increase in feed conversion ratio, lipid peroxidation and serum levels of aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, gamma-glutamyl transferase, lactate dehydrogenase and total bilirubin. In addition, livers of AFB1-supplemented animals showed fatty degeneration with vacuolisation, focal areas of necrosis, mononuclear cells infiltration hyperplasia of bile ducts and sinusoids. A significant increase in the hepatic expression of the biotransformation...
Hepatocellular carcinoma (HCC) is the most common cancer in humans. Despite advances in its treat... more Hepatocellular carcinoma (HCC) is the most common cancer in humans. Despite advances in its treatment, liver cancer remains one of the most difficult cancers to treat. This study aimed to investigate the ameliorative action and potential mechanism of Aspergillus awamori (ASP) administration against the initiation process of liver carcinogenesis induced by diethylnitrosamine (DEN) in male Wistar rats. Seventy-two male rats were divided equally into eight groups as follows, Group 1: untreated control; Group 2: DEN (200 mg/kg bw) intra-peritoneally for the initiation of HCC; Groups 3–5: DEN + ASP at a dose of 1, 0.5, and 0.25 mg/kg bw and groups 6–8: ASP at a dose of 1, 0.5, and 0.25 mg/kg bw. Supplementation of A. awamori significantly lightened the adverse impacts induced by DEN via restoring the leukogram to normal, lowering the elevated serum aspartate aminotransferase (AST), alanine transaminase (ALT), and γ-glutamyl transferase (GGT), and alkaline phosphatase (ALP). Furthermore, ...
Background Collagen is the most abundant structural protein in the mammalian connective tissue an... more Background Collagen is the most abundant structural protein in the mammalian connective tissue and represents approximately 30% of animal protein. The current study evaluated the potential capacity of collagen extract derived from Nile tilapia skin in improving the cutaneous wound healing in rats and investigated the underlying possible mechanisms. A rat model was used, and the experimental design included a control group (CG) and the tilapia collagen treated group (TCG). Full-thickness wounds were conducted on the back of all the rats under general anesthesia, then the tilapia collagen extract was applied topically on the wound area of TCG. Wound areas of the two experimental groups were measured on days 0, 3, 6, 9, 12, and 15 post-wounding. The stages of the wound granulation tissues were detected by histopathologic examination and the expression of vascular endothelial growth factor (VEGF), and transforming growth factor (TGF-ß1) were investigated using immunohistochemistry. More...
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