The contribution of immune system cells to the propagation of transmissible encephalopathies is n... more The contribution of immune system cells to the propagation of transmissible encephalopathies is not well understood. To determine how follicular dendritic cells (FDC) may act, we challenged lymphotoxin β null and wild-type (wt) controls with a Creutzfeldt-Jakob disease (CJD) agent. There was only a small difference in incubation time to clinical disease even after peripheral challenge with low infectious doses (31 in a total of 410 days). Brain pathology with extensive microglial infiltration, identified by keratan sulfate, as well as astrocytic hypertrophy, was also equivalent in all groups despite the fact that null mice had neither FDC nor splenic metallophilic macrophages that filter particulate antigen. Because FDC accumulate pathologic prion protein (PrP) in infected but not wt mice, we studied the cellular distribution of PrP by confocal microscopy. The majority of pathologic PrP collected on the plasma membrane of FDC, as identified by the Ca +2 -binding protein S100A. This ...
Conjunctional Concepts: The Conceptual Teaching Technique in Biology Classes, 2021
Conceptual teaching relies on concepts in conjunction with a bigger conceptual category (or categ... more Conceptual teaching relies on concepts in conjunction with a bigger conceptual category (or categories). Revealing and using conceptual conjunctions in the science classroom means teaching conceptually. To make conceptual teaching more accessible to biology instructors, a three-concept model was described to be used during a lecture. This model has allowed the author to develop a relatively simple method that starts with an active concept or concepts, then introduces conjunctional and supporting concepts to relate the content to other concepts and relevant real-world applications. This method is intended to help instructors in preparing to teach biological concepts conceptually, in order to foster an enduring understanding of biological principles in their students.
1. Lancet. 1998 Aug 8;352(9126):456. Dapsone to delay symptoms in Creutzfeldt-Jakob disease. Manu... more 1. Lancet. 1998 Aug 8;352(9126):456. Dapsone to delay symptoms in Creutzfeldt-Jakob disease. Manuelidis L, Fritch W, Zaitsev I. PMID: 9708762 [PubMed - indexed for MEDLINE]. Publication Types: Letter; Research Support, US Gov't, PHS. MeSH Terms: ...
The contribution of immune system cells to the propagation of transmissible encephalopathies is n... more The contribution of immune system cells to the propagation of transmissible encephalopathies is not well understood. To determine how follicular dendritic cells (FDC) may act, we challenged lymphotoxin β null and wild-type (wt) controls with a Creutzfeldt-Jakob disease (CJD) agent. There was only a small difference in incubation time to clinical disease even after peripheral challenge with low infectious doses (31 in a total of 410 days). Brain pathology with extensive microglial infiltration, identified by keratan sulfate, as well as astrocytic hypertrophy, was also equivalent in all groups despite the fact that null mice had neither FDC nor splenic metallophilic macrophages that filter particulate antigen. Because FDC accumulate pathologic prion protein (PrP) in infected but not wt mice, we studied the cellular distribution of PrP by confocal microscopy. The majority of pathologic PrP collected on the plasma membrane of FDC, as identified by the Ca +2 -binding protein S100A. This ...
Conjunctional Concepts: The Conceptual Teaching Technique in Biology Classes, 2021
Conceptual teaching relies on concepts in conjunction with a bigger conceptual category (or categ... more Conceptual teaching relies on concepts in conjunction with a bigger conceptual category (or categories). Revealing and using conceptual conjunctions in the science classroom means teaching conceptually. To make conceptual teaching more accessible to biology instructors, a three-concept model was described to be used during a lecture. This model has allowed the author to develop a relatively simple method that starts with an active concept or concepts, then introduces conjunctional and supporting concepts to relate the content to other concepts and relevant real-world applications. This method is intended to help instructors in preparing to teach biological concepts conceptually, in order to foster an enduring understanding of biological principles in their students.
1. Lancet. 1998 Aug 8;352(9126):456. Dapsone to delay symptoms in Creutzfeldt-Jakob disease. Manu... more 1. Lancet. 1998 Aug 8;352(9126):456. Dapsone to delay symptoms in Creutzfeldt-Jakob disease. Manuelidis L, Fritch W, Zaitsev I. PMID: 9708762 [PubMed - indexed for MEDLINE]. Publication Types: Letter; Research Support, US Gov't, PHS. MeSH Terms: ...
Uploads