The objectives of the research are: (1) To explore the learning strategies used by the autistic s... more The objectives of the research are: (1) To explore the learning strategies used by the autistic student in writing English text at Al-Firdaus Junior high school of Sukoharjo in the Academic year of 2013/2014, (2) To explore the process of teaching writing English texts to the students in an inclusive class at Al-Firdaus Junior high school of Sukoharjo, in the Academic year of 2013/2014, (3) To investigate the impacts of learning strategies on writing English texts used by the autistic student to the teaching and learning process at Al-Firdaus Junior high school of Sukoharjo in the Academic year of 2013/2014. The research was carried out at Al-Firdaus Junior high school of Sukoharjo. It was conducted from January to March 2014. The subject of the research is the student SA which is classified as a high-functioning autism student. He belongs to class VIIB, grade seventh. It is a case study research. The case study data are collected through document analysis, observation, interview, a...
Cyanobacterial harmful algal blooms (CHABs) are increasing at an alarming rate in different water... more Cyanobacterial harmful algal blooms (CHABs) are increasing at an alarming rate in different water bodies worldwide. In India, CHAB events in water bodies such as Dal Lake have been sporadically reported with no study done to characterize the cyanobacterial species and their associated toxins. We hypothesized that this Lake is contaminated with toxic cyanobacterial species with the possibility of the presence of cyanotoxin biosynthetic genes. We, therefore, used some of the molecular tools such as 16S ribosomal DNA, PCR, and phylogenetic analysis to explore cyanobacterial species and their associated toxins. A 3-year (2018–2020) survey was conducted at three different sampling sites of Dal Lake namely, Grand Palace Gath (S1), Nigeen basin (S2), and Gagribal basin (S3). Two strains of Dolichospermum sp. AE01 and AE02 (S3 and S1 site) and one strain of Microcystis sp. AE03 (S2 site) was isolated, cultured, and characterized phylogenetically by 16S ribosomal DNA sequencing. The presence...
Furin cleavage-site (CS) present between the S1/S2 junction in SARS-CoV2 spike (S) protein is cri... more Furin cleavage-site (CS) present between the S1/S2 junction in SARS-CoV2 spike (S) protein is critical to drive the fusion of SARS-CoV2 with the host cell. SARS-CoV2 falls in the sarbecovirus lineage that doesn’t comprise of furin CS and therefore makes its origin enigmatic. The available wild-type (Wt) SARS-CoV2 S protein with PDB ID: 6yvb lacks a stretch of amino acid including furin CS as well. All investigators till date have shown this stretch existing in the form of a loop. We are for the first time reporting that this stretch comprises of 14 amino acid residues (677QTNSPRRARSVASQ689), forming an antiparallel β-sheet comprising of PRRAR furin CS. We observed the presence of this antiparallel β-sheet in MERS spike protein as well. While switching over from Wt. SARS-CoV2 with PRRAR furin CS to B.1.1.7 variant with HRRAR furin CS, we found 3% increase in the percentage content of β stands. Interestingly, we found that the change of B.1.1.7 to B.1.617 variant comprising of RRRAR f...
ABSTRACT Gusset plate connections are commonly used in bridge trusses and braced steel frames to ... more ABSTRACT Gusset plate connections are commonly used in bridge trusses and braced steel frames to transfer forces from one structural member to another. Due to the complexity of these connections, it is extremely difficult to evaluate the strength of gusset plate connections. ...
Unfolded protein response is a dynamic signalling pathway, which is involved in the maintenance o... more Unfolded protein response is a dynamic signalling pathway, which is involved in the maintenance of proteostasis and cellular homeostasis. IRE1, a transmembrane signalling protein represents the start point of a highly conserved UPR signalling cascade. IRE1 is endowed with kinase and endoribonuclease activities. The activation of the kinase domain of IRE1 by trans-autophosphorylation leads to the activation of its RNAse domain. RNAse domain performs atypical splicing of Xbp1 mRNA and degradation of mRNAs by an effector function known as Regulated IRE1 Dependent Decay (RIDD). The regulation of the distinctive nature of the IRE1 ribonuclease function is potentially mediated by a dynamic protein structure UPRosome that is an assembly of a huge number of proteins on IRE1. Here, we reported that Bid is a novel recruit to UPRosome, which directly interacts with the cytoplasmic domain of IRE1. Bid controls the auto-phosphorylation of IRE1 in a negative manner where Bid overexpression condit...
Angiogenesis and cell proliferation are the major pathological components of cancer. Angiogenesis... more Angiogenesis and cell proliferation are the major pathological components of cancer. Angiogenesis means the growth of neovessels from the existing vessels. It is tightly regulated by various inhibitors and stimulators in vivo and takes place at the time of embryogenesis and wound healing. It is primarily recruited by the solid tumors for receiving more nutrients and oxygen for the continuously dividing cells. Proliferation is the pathway from neoplasm to hyperplasm and ultimately leads to metastasis. These days they are attractive targets to cure tumors and malignancy. Wide ranges of plants contain many bioactive compounds documented for antiangiogenic and antiproliferative property. Anthocyanin is one of the bioactive ingredients documented for possessing antiangiogenic and antiproliferating potential. Anthocyanin containing Solanum melongena, Brassica olerecea and Amaranthus cruentus investigated for their antiangiogenic and antiproliferative potential utilizing Chorioallantoic me...
.............................................................................................3 Ch... more .............................................................................................3 Chronic obstructive pulmonary disease.......................................... ...............3-4 Epidemiology.......................................................................................4 Molecular genetics.................................................................................4-5 Pathogenesis........................................................................................6-9 Mechanism of parenchymal lung disease................................................6 Loss of protease-antiprotease homeostasis... ..................................................6 ZAAT-induced liver and lung disease..................... .....................................7-9 SERPINA1 gene polymorphism.................................................................9-11 Novel SERPINA1 Null mutations .............................................................11 Q0Granite Falls....................................................................................12-13 Q0ludwigshafen...................................................................................13 Q0ourem.........................................................................................13 Q0porto..........................................................................................14 Q0Cairo..........................................................................................14 Q0soest and Q0amersfoort ........................................................................15 Q0brescia.........................................................................................15 Q0Milano.........................................................................................16 Q0Madrid.........................................................................................16-17 Other novel Null variants of alpha-1 antit rypsin .............................................17-18 Dinucleotide insertion in the exon II of the SERPINA1 gene...........................18 Mutations affecting the C-terminal region o f alpha1-antitrypsin...........................19-21 Mpisa, Etaurisano and Yorzinuovi variants of alpha1 -antitrypsin.....................19-20 T379∆ variant of alpha1-antitrypsin.......................................................20 -21 Conclusion..........................................................................................2122 References..........................................................................................2431 M AN US CR IP T AC CE PT ED ACCEPTED MANUSCRIPT
Different mutations in coding and non-coding sequences of the SERPINA1 gene have been implicated ... more Different mutations in coding and non-coding sequences of the SERPINA1 gene have been implicated in the pathogenesis of COPD. However, - 10T/C mutation in the hepatocyte-directed promoter region has not been associated with COPD pathogenesis so far. Here, we report an increased frequency of - 10C genotype that is associated with decreased levels of serum alpha1-antitrypsin (α1AT) in COPD patients. The quantification of serum α1AT was done by ELISA, the phenol-chloroform method was used for DNA extraction, PCR products were directly sequenced. The IBM SPSS Statistics v21 software was used for statistical analyses of the data. The mean serum α1AT level was found to be 1.203+0.239 and 3.162+0.160 g/L in COPD cases and in control, respectively. The - 10C allele is associated with an increased risk of COPD [OR, 3.50 (95%CI, 1.86-6.58); p < 0.001]. The combined variant genotype (TT+CC) was significantly found associated with an increased risk of COPD [OR, 3.20 (95% CI, 1.47-6.96); p = ...
A set of cellular response to counter any alteration in homeostasis of a cell originating at endo... more A set of cellular response to counter any alteration in homeostasis of a cell originating at endoplasmic reticulum is collectively termed as unfolded protein response (UPR). It initially is adaptive in nature as to restore cellular normalcy failing in course often activates pro-apoptotic signaling pathway resulting in cell death. UPR has emerged as an essential adaptation mechanism that cross talk with various cellular processes for cancer pathogenesis. Interestingly, it plays diverse role in plethora of signaling pathways instrumental in transformation, cell invasion, cell migration, metastasis, neovascularization, proliferation, and maintenance of energy metabolism of cancerous cells. In cancerous cells, it is triggered by change in microenvironment of a cell usually driven by hypoxia, acidosis, and nutrient deprivation, which often leads to positive selection pressure involving the reprogramming of energy metabolism which promotes channelization of limited metabolites into the hexosamine biosynthetic pathway (HBP). Substantial evidences suggest the role of UPR in oncogene (Myc, mTOR, RAS, HER2) driven cancer transformation and progression. In this review, we have comprehensively underlined the role played by UPR in adaptation, transformation, proliferation, invasion, and metastasis of cancerous cells.
The objectives of the research are: (1) To explore the learning strategies used by the autistic s... more The objectives of the research are: (1) To explore the learning strategies used by the autistic student in writing English text at Al-Firdaus Junior high school of Sukoharjo in the Academic year of 2013/2014, (2) To explore the process of teaching writing English texts to the students in an inclusive class at Al-Firdaus Junior high school of Sukoharjo, in the Academic year of 2013/2014, (3) To investigate the impacts of learning strategies on writing English texts used by the autistic student to the teaching and learning process at Al-Firdaus Junior high school of Sukoharjo in the Academic year of 2013/2014. The research was carried out at Al-Firdaus Junior high school of Sukoharjo. It was conducted from January to March 2014. The subject of the research is the student SA which is classified as a high-functioning autism student. He belongs to class VIIB, grade seventh. It is a case study research. The case study data are collected through document analysis, observation, interview, a...
Cyanobacterial harmful algal blooms (CHABs) are increasing at an alarming rate in different water... more Cyanobacterial harmful algal blooms (CHABs) are increasing at an alarming rate in different water bodies worldwide. In India, CHAB events in water bodies such as Dal Lake have been sporadically reported with no study done to characterize the cyanobacterial species and their associated toxins. We hypothesized that this Lake is contaminated with toxic cyanobacterial species with the possibility of the presence of cyanotoxin biosynthetic genes. We, therefore, used some of the molecular tools such as 16S ribosomal DNA, PCR, and phylogenetic analysis to explore cyanobacterial species and their associated toxins. A 3-year (2018–2020) survey was conducted at three different sampling sites of Dal Lake namely, Grand Palace Gath (S1), Nigeen basin (S2), and Gagribal basin (S3). Two strains of Dolichospermum sp. AE01 and AE02 (S3 and S1 site) and one strain of Microcystis sp. AE03 (S2 site) was isolated, cultured, and characterized phylogenetically by 16S ribosomal DNA sequencing. The presence...
Furin cleavage-site (CS) present between the S1/S2 junction in SARS-CoV2 spike (S) protein is cri... more Furin cleavage-site (CS) present between the S1/S2 junction in SARS-CoV2 spike (S) protein is critical to drive the fusion of SARS-CoV2 with the host cell. SARS-CoV2 falls in the sarbecovirus lineage that doesn’t comprise of furin CS and therefore makes its origin enigmatic. The available wild-type (Wt) SARS-CoV2 S protein with PDB ID: 6yvb lacks a stretch of amino acid including furin CS as well. All investigators till date have shown this stretch existing in the form of a loop. We are for the first time reporting that this stretch comprises of 14 amino acid residues (677QTNSPRRARSVASQ689), forming an antiparallel β-sheet comprising of PRRAR furin CS. We observed the presence of this antiparallel β-sheet in MERS spike protein as well. While switching over from Wt. SARS-CoV2 with PRRAR furin CS to B.1.1.7 variant with HRRAR furin CS, we found 3% increase in the percentage content of β stands. Interestingly, we found that the change of B.1.1.7 to B.1.617 variant comprising of RRRAR f...
ABSTRACT Gusset plate connections are commonly used in bridge trusses and braced steel frames to ... more ABSTRACT Gusset plate connections are commonly used in bridge trusses and braced steel frames to transfer forces from one structural member to another. Due to the complexity of these connections, it is extremely difficult to evaluate the strength of gusset plate connections. ...
Unfolded protein response is a dynamic signalling pathway, which is involved in the maintenance o... more Unfolded protein response is a dynamic signalling pathway, which is involved in the maintenance of proteostasis and cellular homeostasis. IRE1, a transmembrane signalling protein represents the start point of a highly conserved UPR signalling cascade. IRE1 is endowed with kinase and endoribonuclease activities. The activation of the kinase domain of IRE1 by trans-autophosphorylation leads to the activation of its RNAse domain. RNAse domain performs atypical splicing of Xbp1 mRNA and degradation of mRNAs by an effector function known as Regulated IRE1 Dependent Decay (RIDD). The regulation of the distinctive nature of the IRE1 ribonuclease function is potentially mediated by a dynamic protein structure UPRosome that is an assembly of a huge number of proteins on IRE1. Here, we reported that Bid is a novel recruit to UPRosome, which directly interacts with the cytoplasmic domain of IRE1. Bid controls the auto-phosphorylation of IRE1 in a negative manner where Bid overexpression condit...
Angiogenesis and cell proliferation are the major pathological components of cancer. Angiogenesis... more Angiogenesis and cell proliferation are the major pathological components of cancer. Angiogenesis means the growth of neovessels from the existing vessels. It is tightly regulated by various inhibitors and stimulators in vivo and takes place at the time of embryogenesis and wound healing. It is primarily recruited by the solid tumors for receiving more nutrients and oxygen for the continuously dividing cells. Proliferation is the pathway from neoplasm to hyperplasm and ultimately leads to metastasis. These days they are attractive targets to cure tumors and malignancy. Wide ranges of plants contain many bioactive compounds documented for antiangiogenic and antiproliferative property. Anthocyanin is one of the bioactive ingredients documented for possessing antiangiogenic and antiproliferating potential. Anthocyanin containing Solanum melongena, Brassica olerecea and Amaranthus cruentus investigated for their antiangiogenic and antiproliferative potential utilizing Chorioallantoic me...
.............................................................................................3 Ch... more .............................................................................................3 Chronic obstructive pulmonary disease.......................................... ...............3-4 Epidemiology.......................................................................................4 Molecular genetics.................................................................................4-5 Pathogenesis........................................................................................6-9 Mechanism of parenchymal lung disease................................................6 Loss of protease-antiprotease homeostasis... ..................................................6 ZAAT-induced liver and lung disease..................... .....................................7-9 SERPINA1 gene polymorphism.................................................................9-11 Novel SERPINA1 Null mutations .............................................................11 Q0Granite Falls....................................................................................12-13 Q0ludwigshafen...................................................................................13 Q0ourem.........................................................................................13 Q0porto..........................................................................................14 Q0Cairo..........................................................................................14 Q0soest and Q0amersfoort ........................................................................15 Q0brescia.........................................................................................15 Q0Milano.........................................................................................16 Q0Madrid.........................................................................................16-17 Other novel Null variants of alpha-1 antit rypsin .............................................17-18 Dinucleotide insertion in the exon II of the SERPINA1 gene...........................18 Mutations affecting the C-terminal region o f alpha1-antitrypsin...........................19-21 Mpisa, Etaurisano and Yorzinuovi variants of alpha1 -antitrypsin.....................19-20 T379∆ variant of alpha1-antitrypsin.......................................................20 -21 Conclusion..........................................................................................2122 References..........................................................................................2431 M AN US CR IP T AC CE PT ED ACCEPTED MANUSCRIPT
Different mutations in coding and non-coding sequences of the SERPINA1 gene have been implicated ... more Different mutations in coding and non-coding sequences of the SERPINA1 gene have been implicated in the pathogenesis of COPD. However, - 10T/C mutation in the hepatocyte-directed promoter region has not been associated with COPD pathogenesis so far. Here, we report an increased frequency of - 10C genotype that is associated with decreased levels of serum alpha1-antitrypsin (α1AT) in COPD patients. The quantification of serum α1AT was done by ELISA, the phenol-chloroform method was used for DNA extraction, PCR products were directly sequenced. The IBM SPSS Statistics v21 software was used for statistical analyses of the data. The mean serum α1AT level was found to be 1.203+0.239 and 3.162+0.160 g/L in COPD cases and in control, respectively. The - 10C allele is associated with an increased risk of COPD [OR, 3.50 (95%CI, 1.86-6.58); p < 0.001]. The combined variant genotype (TT+CC) was significantly found associated with an increased risk of COPD [OR, 3.20 (95% CI, 1.47-6.96); p = ...
A set of cellular response to counter any alteration in homeostasis of a cell originating at endo... more A set of cellular response to counter any alteration in homeostasis of a cell originating at endoplasmic reticulum is collectively termed as unfolded protein response (UPR). It initially is adaptive in nature as to restore cellular normalcy failing in course often activates pro-apoptotic signaling pathway resulting in cell death. UPR has emerged as an essential adaptation mechanism that cross talk with various cellular processes for cancer pathogenesis. Interestingly, it plays diverse role in plethora of signaling pathways instrumental in transformation, cell invasion, cell migration, metastasis, neovascularization, proliferation, and maintenance of energy metabolism of cancerous cells. In cancerous cells, it is triggered by change in microenvironment of a cell usually driven by hypoxia, acidosis, and nutrient deprivation, which often leads to positive selection pressure involving the reprogramming of energy metabolism which promotes channelization of limited metabolites into the hexosamine biosynthetic pathway (HBP). Substantial evidences suggest the role of UPR in oncogene (Myc, mTOR, RAS, HER2) driven cancer transformation and progression. In this review, we have comprehensively underlined the role played by UPR in adaptation, transformation, proliferation, invasion, and metastasis of cancerous cells.
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