Snehith has completed B.Pharmacy in NRI college of Pharmacy, Vijayawada, and M.Pharmacy in Jawaharlal Nehru Technological University Anantapur, Anantapuramu, Andhra Pradesh. He has published seven research papers in reputed journals. He has also presented poster presentations at National and International conferences. Presently, he is working in Biophore India Pharmaceutical Pvt.Ltd as a Quality Assurance. He also managing SS Pharma (www.spharma.ind.in) and Director of Pharmascrutiny (www.pharmascrutiny.in). Supervisors: B.Sivasaikiran and O. Srinivasa Krishna Phone: 964*****55 Address: Kanuru, Vijayawada-520007
The objective of work was to develop and validate a UV
spectrophotometric method for deferiprone ... more The objective of work was to develop and validate a UV spectrophotometric method for deferiprone in bulk and its dosage form. The solvent and wavelength of detection were optimized in order to maximize sensitivity of proposed method. The method was validated for different parameters like linearity, precision, specificity, accuracy, limit of detection (LOD), limit of quantitation (LOQ) and robustness as per ICH guidelines (Q2). A wavelength maximum absorption of Deferiprone in 50% v/v ethanol was monitored at 278nm. The method was found to be linear in the range of 2 to 12μg/ml with a correlation coefficient (R2 ) of 0.999. The accuracy of the method was studied by recovery study and % recovery was found to be 101.07%. The LOD and LOQ were found to be 0.1808μg/ml and 0.547μg/ml respectively. The method is simple, accurate and requires relatively inexpensive instrument. The method was used successfully for determination of Deferiprone in bulk and its pharmaceutical dosage form
World Journal of Pharmaceutical and Life Sciences, 2019
A simple, rapid, accurate, precise, selective and economical UV Spectrophotometric method has bee... more A simple, rapid, accurate, precise, selective and economical UV Spectrophotometric method has been developed for estimation of Simvastatin in bulk and its dosage form. Ethanol 30% v/v was used as diluent for the study. The maximum absorption was monitored at 238 nm. The method exhibited linearity in the concentration range of 5- 30µg/ml with correlation coefficient value 0.999. The accuracy of the method was checked at three different levels of 50%, 100% and 150 %. The % recovery was found to be 100.18%. The developed method was validated with respect to accuracy, precision, selectivity, specificity and robustness. All the parameters examined were within the acceptance limits.The above method was a cost-effective quality control tool for routine analysis of Simvastatin in bulk and its pharmaceutical dosage form.
Journal of Global Trends in Pharmaceutical Sciences, 2019
ARTICLE INFO ABSTRACT Key Words Vancomycin, ethanol, Distilled water, ICH guidelines An UV-Spectr... more ARTICLE INFO ABSTRACT Key Words Vancomycin, ethanol, Distilled water, ICH guidelines An UV-Spectroscopic validated method has been developedfor the determination of Vancomycin. 50% ethanol and 50% Distilled waterused as mobile phase composition. The method produced linear response over the wide concentration range of 25-150 Micro gram/Ml, with an average accuracy of 99.55%, as well as average intra-and inter-day variations of 1.0398 and 1.1676 %, respectively. The limits of detection and Quantitation of the method were 3.96 Micro grams / Ml and 12 Micro gram/Ml respectively.
International Journal of Scientific Engineering and Science, 2019
The aim of the present research was broadly focused on the estimation of carbamazepine in bulk an... more The aim of the present research was broadly focused on the estimation of carbamazepine in bulk and pharmaceutical dosage form by using two UV-Spectrophotometric methods namely, Zero order UV spectrophotometry (Method-1) and Area under the curve UV spectrophotometry (Method-2). The Zero order UV Spectrophotometric method was based on the measurement drug absorbance at wave length of 284 nm, which was its wavelength of maximum absorbance .The Area under the curve method, was based on the calculation of area occupied by the UV absorbance curve between 278-290 nm. The solvent employed for both methods was 50% v/v ethanol. In the estimation of Carbamazepine, both the methods showed linearity in the range of 2-12µg/ml. The correlation coefficient was ≥ 0.999. The precision for both the methods was ≤2% RSD. The accuracy was performed by using percentage recovery studies of standard drug spiked at 50,100 and 150% of the test concentration and the values obtained were within the limits. The developed methods were applied for the assay of the drug in its respective dosage forms. The assay of pharmaceuticals dosage form was found to be within limits. All the results were satisfactory, the developed methods can be routinely used for the analysis of the drugs in both bulk and dosage forms.
International Journal of Pharma Research and Health Sciences, 2019
: The main objective of present research work simple, accurate and precise UV
Spectroscopic metho... more : The main objective of present research work simple, accurate and precise UV Spectroscopic method was developed for the determination of purity of Clopidogrel Bisulphate. Experimental approach: The method was developed using Shimadzu 1800 UV- Visible double beam spectrophotometer with UV probe software with Ethanol as a solvent. The wavelength is monitored at 218 nm. The developed method was validated as per ICH guidelines. Results: The method produced linear response over the wide concentration range of 2-12 µg/mL, with an average accuracy of 99.06%, as well as average intra- and inter-day variations of 0.528 and 0.624 %, respectively. Conclusion: The developed method was found to be precise, robust and accurate. This method can be suggest to routine quality control analysis of Clopidogrel bisulphate in its pure and pharmaceutical formulation.
A novel UV Spectroscopic method was developed for the determination of purity of Indomethacin. Th... more A novel UV Spectroscopic method was developed for the determination of purity of Indomethacin. The method was developed using Shimadzu 1800 UV-Visible double beam spectrophotometer with uv probe software with Ethanol used as solvent. The compound is monitored at 266 nm. The developed method was validated as per ICH guidelines with respect to specicity, linearity, limit of detection, limit of quantication, accuracy, precision and robustness. This method was also suitable for the assay determination of Indomethacin in pharmaceutical dosage forms.
The objective of work was to develop and validate a UV
spectrophotometric method for deferiprone ... more The objective of work was to develop and validate a UV spectrophotometric method for deferiprone in bulk and its dosage form. The solvent and wavelength of detection were optimized in order to maximize sensitivity of proposed method. The method was validated for different parameters like linearity, precision, specificity, accuracy, limit of detection (LOD), limit of quantitation (LOQ) and robustness as per ICH guidelines (Q2). A wavelength maximum absorption of Deferiprone in 50% v/v ethanol was monitored at 278nm. The method was found to be linear in the range of 2 to 12μg/ml with a correlation coefficient (R2 ) of 0.999. The accuracy of the method was studied by recovery study and % recovery was found to be 101.07%. The LOD and LOQ were found to be 0.1808μg/ml and 0.547μg/ml respectively. The method is simple, accurate and requires relatively inexpensive instrument. The method was used successfully for determination of Deferiprone in bulk and its pharmaceutical dosage form
World Journal of Pharmaceutical and Life Sciences, 2019
A simple, rapid, accurate, precise, selective and economical UV Spectrophotometric method has bee... more A simple, rapid, accurate, precise, selective and economical UV Spectrophotometric method has been developed for estimation of Simvastatin in bulk and its dosage form. Ethanol 30% v/v was used as diluent for the study. The maximum absorption was monitored at 238 nm. The method exhibited linearity in the concentration range of 5- 30µg/ml with correlation coefficient value 0.999. The accuracy of the method was checked at three different levels of 50%, 100% and 150 %. The % recovery was found to be 100.18%. The developed method was validated with respect to accuracy, precision, selectivity, specificity and robustness. All the parameters examined were within the acceptance limits.The above method was a cost-effective quality control tool for routine analysis of Simvastatin in bulk and its pharmaceutical dosage form.
Journal of Global Trends in Pharmaceutical Sciences, 2019
ARTICLE INFO ABSTRACT Key Words Vancomycin, ethanol, Distilled water, ICH guidelines An UV-Spectr... more ARTICLE INFO ABSTRACT Key Words Vancomycin, ethanol, Distilled water, ICH guidelines An UV-Spectroscopic validated method has been developedfor the determination of Vancomycin. 50% ethanol and 50% Distilled waterused as mobile phase composition. The method produced linear response over the wide concentration range of 25-150 Micro gram/Ml, with an average accuracy of 99.55%, as well as average intra-and inter-day variations of 1.0398 and 1.1676 %, respectively. The limits of detection and Quantitation of the method were 3.96 Micro grams / Ml and 12 Micro gram/Ml respectively.
International Journal of Scientific Engineering and Science, 2019
The aim of the present research was broadly focused on the estimation of carbamazepine in bulk an... more The aim of the present research was broadly focused on the estimation of carbamazepine in bulk and pharmaceutical dosage form by using two UV-Spectrophotometric methods namely, Zero order UV spectrophotometry (Method-1) and Area under the curve UV spectrophotometry (Method-2). The Zero order UV Spectrophotometric method was based on the measurement drug absorbance at wave length of 284 nm, which was its wavelength of maximum absorbance .The Area under the curve method, was based on the calculation of area occupied by the UV absorbance curve between 278-290 nm. The solvent employed for both methods was 50% v/v ethanol. In the estimation of Carbamazepine, both the methods showed linearity in the range of 2-12µg/ml. The correlation coefficient was ≥ 0.999. The precision for both the methods was ≤2% RSD. The accuracy was performed by using percentage recovery studies of standard drug spiked at 50,100 and 150% of the test concentration and the values obtained were within the limits. The developed methods were applied for the assay of the drug in its respective dosage forms. The assay of pharmaceuticals dosage form was found to be within limits. All the results were satisfactory, the developed methods can be routinely used for the analysis of the drugs in both bulk and dosage forms.
International Journal of Pharma Research and Health Sciences, 2019
: The main objective of present research work simple, accurate and precise UV
Spectroscopic metho... more : The main objective of present research work simple, accurate and precise UV Spectroscopic method was developed for the determination of purity of Clopidogrel Bisulphate. Experimental approach: The method was developed using Shimadzu 1800 UV- Visible double beam spectrophotometer with UV probe software with Ethanol as a solvent. The wavelength is monitored at 218 nm. The developed method was validated as per ICH guidelines. Results: The method produced linear response over the wide concentration range of 2-12 µg/mL, with an average accuracy of 99.06%, as well as average intra- and inter-day variations of 0.528 and 0.624 %, respectively. Conclusion: The developed method was found to be precise, robust and accurate. This method can be suggest to routine quality control analysis of Clopidogrel bisulphate in its pure and pharmaceutical formulation.
A novel UV Spectroscopic method was developed for the determination of purity of Indomethacin. Th... more A novel UV Spectroscopic method was developed for the determination of purity of Indomethacin. The method was developed using Shimadzu 1800 UV-Visible double beam spectrophotometer with uv probe software with Ethanol used as solvent. The compound is monitored at 266 nm. The developed method was validated as per ICH guidelines with respect to specicity, linearity, limit of detection, limit of quantication, accuracy, precision and robustness. This method was also suitable for the assay determination of Indomethacin in pharmaceutical dosage forms.
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Papers by Boddu Snehith
spectrophotometric method for deferiprone in bulk and its dosage
form. The solvent and wavelength of detection were optimized in order
to maximize sensitivity of proposed method. The method was validated
for different parameters like linearity, precision, specificity, accuracy,
limit of detection (LOD), limit of quantitation (LOQ) and robustness as
per ICH guidelines (Q2). A wavelength maximum absorption of
Deferiprone in 50% v/v ethanol was monitored at 278nm. The method
was found to be linear in the range of 2 to 12μg/ml with a correlation
coefficient (R2
) of 0.999. The accuracy of the method was studied by
recovery study and % recovery was found to be 101.07%. The LOD
and LOQ were found to be 0.1808μg/ml and 0.547μg/ml respectively.
The method is simple, accurate and requires relatively inexpensive
instrument. The method was used successfully for determination of
Deferiprone in bulk and its pharmaceutical dosage form
for estimation of Simvastatin in bulk and its dosage form. Ethanol 30% v/v was used as diluent for the study. The
maximum absorption was monitored at 238 nm. The method exhibited linearity in the concentration range of 5-
30µg/ml with correlation coefficient value 0.999. The accuracy of the method was checked at three different levels
of 50%, 100% and 150 %. The % recovery was found to be 100.18%. The developed method was validated with
respect to accuracy, precision, selectivity, specificity and robustness. All the parameters examined were within the
acceptance limits.The above method was a cost-effective quality control tool for routine analysis of Simvastatin in
bulk and its pharmaceutical dosage form.
Spectroscopic method was developed for the determination of purity of Clopidogrel
Bisulphate. Experimental approach: The method was developed using Shimadzu 1800 UV- Visible double beam spectrophotometer with UV probe software with Ethanol as a solvent.
The wavelength is monitored at 218 nm. The developed method was validated as per ICH
guidelines. Results: The method produced linear response over the wide concentration range
of 2-12 µg/mL, with an average accuracy of 99.06%, as well as average intra- and inter-day
variations of 0.528 and 0.624 %, respectively.
Conclusion: The developed method was found to be precise, robust and accurate. This
method can be suggest to routine quality control analysis of Clopidogrel bisulphate in its
pure and pharmaceutical formulation.
developed using Shimadzu 1800 UV-Visible double beam spectrophotometer with uv probe software with Ethanol
used as solvent. The compound is monitored at 266 nm. The developed method was validated as per ICH guidelines with respect to
specicity, linearity, limit of detection, limit of quantication, accuracy, precision and robustness. This method was also suitable for the assay
determination of Indomethacin in pharmaceutical dosage forms.
spectrophotometric method for deferiprone in bulk and its dosage
form. The solvent and wavelength of detection were optimized in order
to maximize sensitivity of proposed method. The method was validated
for different parameters like linearity, precision, specificity, accuracy,
limit of detection (LOD), limit of quantitation (LOQ) and robustness as
per ICH guidelines (Q2). A wavelength maximum absorption of
Deferiprone in 50% v/v ethanol was monitored at 278nm. The method
was found to be linear in the range of 2 to 12μg/ml with a correlation
coefficient (R2
) of 0.999. The accuracy of the method was studied by
recovery study and % recovery was found to be 101.07%. The LOD
and LOQ were found to be 0.1808μg/ml and 0.547μg/ml respectively.
The method is simple, accurate and requires relatively inexpensive
instrument. The method was used successfully for determination of
Deferiprone in bulk and its pharmaceutical dosage form
for estimation of Simvastatin in bulk and its dosage form. Ethanol 30% v/v was used as diluent for the study. The
maximum absorption was monitored at 238 nm. The method exhibited linearity in the concentration range of 5-
30µg/ml with correlation coefficient value 0.999. The accuracy of the method was checked at three different levels
of 50%, 100% and 150 %. The % recovery was found to be 100.18%. The developed method was validated with
respect to accuracy, precision, selectivity, specificity and robustness. All the parameters examined were within the
acceptance limits.The above method was a cost-effective quality control tool for routine analysis of Simvastatin in
bulk and its pharmaceutical dosage form.
Spectroscopic method was developed for the determination of purity of Clopidogrel
Bisulphate. Experimental approach: The method was developed using Shimadzu 1800 UV- Visible double beam spectrophotometer with UV probe software with Ethanol as a solvent.
The wavelength is monitored at 218 nm. The developed method was validated as per ICH
guidelines. Results: The method produced linear response over the wide concentration range
of 2-12 µg/mL, with an average accuracy of 99.06%, as well as average intra- and inter-day
variations of 0.528 and 0.624 %, respectively.
Conclusion: The developed method was found to be precise, robust and accurate. This
method can be suggest to routine quality control analysis of Clopidogrel bisulphate in its
pure and pharmaceutical formulation.
developed using Shimadzu 1800 UV-Visible double beam spectrophotometer with uv probe software with Ethanol
used as solvent. The compound is monitored at 266 nm. The developed method was validated as per ICH guidelines with respect to
specicity, linearity, limit of detection, limit of quantication, accuracy, precision and robustness. This method was also suitable for the assay
determination of Indomethacin in pharmaceutical dosage forms.