Research journal of pharmacy and technology, Dec 28, 2021
Alcea setosa A. (Malvaceae) is a wild plant that grows in Jordan and have several traditional med... more Alcea setosa A. (Malvaceae) is a wild plant that grows in Jordan and have several traditional medicinal uses. This study aims to collect and chemically analyze the methanolic extract from Alcea setosa A. from Jordan and to evaluate its cytotoxic and antioxidant activity against human breast cancer cells (T47D), colorectal adenocarcinoma cells (CACO2), and normal human fibroblasts (MRC5). The extract was extracted by methanol solvent and analyzed by liquid chromatography coupled with a mass spectrometer. Cell viability was assessed using trypan blue, neutral red, and MTT assays, and antioxidant activity was evaluated using DPPH scavenging activity assay. A total of 290 compounds, 12 among which were identified when compared to available standards, the extract contained six flavons derivatives, Two fatty acids, one ketone derivative, one flavonol derivative, one organic acid, and one coumarin derivative. The results also revealed that the IC50 values of the viability assays were higher among normal cells compared to the human cancer cell lines, and the viability inhibition was significant at higher concentrations compared to untreated cells. Nevertheless, moderate antioxidant activity was observed for the extract in the DPPH scavenging activity test. To sum up, this study indicates that samples of A. setosa collected from Jordan is likely to be an effective antioxidant, is optimistically potential to be utilized in breast and colon cancers treatment due to its preferential cytotoxicity against cancer cells.
Research journal of pharmacy and technology, Jan 25, 2022
Being multipurpose, relatively safe, and widely favorable for consumption, interest in the essent... more Being multipurpose, relatively safe, and widely favorable for consumption, interest in the essential oils of medicinal plants has been increasing. Essential oil (EO) of Juniperus phoenicea is traditionally used for treatment of several health problems such as diabetes, rheumatism, and cancer that is the second leading cause of death in Jordan. This study aims to collect and chemically analyze EO from Juniperus phoenicea L., from Jordan and to evaluate its cytotoxic and antioxidant activity against human breast cancer cells (T47D), colorectal adenocarcinoma cells (CACO2), and normal human fibroblasts (MRC5). EO was extracted by hydro-distillation and analyzed in a gas chromatograph coupled with a mass spectrometer. Cell viability was assessed using trypan blue, neutral red, and MTT assays, and antioxidant activity was evaluated using DPPH scavenging activity assay. Chemical composition analysis revealed 23 constituents in the EO, and the amount of α- pinene was the highest (69.71%). The results also revealed that the IC50 values of the viability assays were higher among normal cells compared to the human cancer cell lines, and the viability inhibition was significant at higher concentrations compared to untreated cells. Nevertheless, low antioxidant activity was observed for the oil in the DPPH scavenging activity test. To sum up, this study indicates that Jordanian Juniperus phoenicea EO, albeit unlikely to be an effective antioxidant, is optimistically potential to be utilized in breast and colon cancers treatment due to its preferential cytotoxicity against cancer cells.
Research journal of pharmacy and technology, Jan 25, 2022
The diversity in Jordan’s flora due to its geographical areas make is well noted in the scientifi... more The diversity in Jordan’s flora due to its geographical areas make is well noted in the scientific literature. The challenge of disease and death caused by infectious diseases like viruses and bacteria, and as infectious diseases evolve and pathogens develop resistance to existing pharmaceuticals, the search for new novel leads, possibly with different modes of action, against bacterial and viral diseases has intensified in recent years. The intent of this review is to provide prevalent information on the antibacterial and antiviral potential in medicinal plants in Jordan, mode of action, type of viruses and bacteria, and phytochemical contents. It has been demonstrated by several studies presented in this review that medicinal plants in Jordan are rich in phytochemicals and possess antiviral and antibacterial properties.
Context: Recent scientific studies have reported that essential oils induce apoptosis in various ... more Context: Recent scientific studies have reported that essential oils induce apoptosis in various cancer cell types by interfering with intracellular signaling pathways. Aims: To evaluate the cytotoxicity, the apoptotic activity of essential oil (EO) of Ruta chalepensis L. against MCF-7 cell line. Methods: Cytotoxicity was determined using methyl thiazol tetrazolium assay. The apoptotic activity of EO was analyzed using annexin V-fluorescein isothiocyanate/propidium iodide binding flow cytometry. The cell morphology was inspected under an inverted microscope. DAPI staining assay was used for the morphological observation. Activation of caspases-3/7, -8, and-9 was assessed using a caspase assay kit. Results: Ruta chalepensis essential oil significantly inhibited the proliferation of MCF-7 cells at 72 h. Moreover, the results showed that cell death is associated with the apoptotic process, and the number of apoptotic cells was significantly increased in the groups treated with EO than ...
Context: Ajuga orientalis L. (Lamiaceae) is an aromatic herb used in traditional medicine in Jord... more Context: Ajuga orientalis L. (Lamiaceae) is an aromatic herb used in traditional medicine in Jordan and neighboring countries. Aims: To evaluate the cytotoxic potential of ethanolic and aqueous extracts from the aerial parts of A. orientalis against three cell lines MCF-7, Caco-2, and HDFa. In addition to assessing the total phenolic and flavonoid contents, antioxidant activity. Further to analyze the phytochemical constituents. Methods: The phytochemical analysis was performed using gas chromatography/mass spectrometry. The total phenolic (TPC) and flavonoid (TFC) contents were assessed using colorimetric methods. The antioxidant properties of both extracts were assessed using 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity and reducing power ability. Cytotoxicity was investigated using methyl thiazol tetrazolium (MTT) assay. Results: The most abundant compounds in the extract were 9-octadecenoic acid, methyl ester, (E)- (27.2%), hexadecanoic acid, methyl ester (12...
Background: One of the primary health concerns for diabetes individuals is wounds. The used drugs... more Background: One of the primary health concerns for diabetes individuals is wounds. The used drugs have several side effects, urging the need for new natural sources for therapeutics. Materials and methods: This study was designed to estimate the wound healing potential of the methanolic extract of Globularia arabica and Malva sylvestris leaves and Rhus coriaria fruits. plant extracts were orally administered to the rats to determine their effect on the wound-healing process. Results: Plant extracts significantly increased the contraction of the wound in non-diabetic and diabetic rats (P < 0.05) and increased the fibroblast's proliferation and migration resulting in a faster healing process. The plant extracts have no cytotoxic effects. The proliferation assay exhibited the lowest cell mortality after treatment with plant extract. Conclusion: These findings may indicate that the methanolic leaf extract of the above plants can be used as new therapeutics for wound healing in diabetic patients.
AimThis study assessed the effects of topical application of R. coriaria extract on the rate of w... more AimThis study assessed the effects of topical application of R. coriaria extract on the rate of wound closure. The rate of wound contraction was used to assess the wound healing efficacy of the R. coriaria fruit methanolic extract.MethodsUsing excision and burn model of wound repair in diabetic male Wistar rats. Also, hydroxyproline, collagen content, and proinflammatory and anti‐inflammatory cytokines levels were determined in this study.ResultsDuring the early wound healing phase, interleukin 6 (IL‐6) levels were found to be decreased by R. coriaria treatment and increased the level of interleukin 10 (IL‐10). Increased wound contraction augmented with hydroxyproline and collagen content, supporting the early wound healing exhibited by R. coriaria. The epithelialization, neovascularization and enhanced hydroxyproline and collagen expression were strongly associated with the healing pattern.ConclusionThis study indicating that R. coriaria methanolic fruit extract has a potent wound ...
Echinococcosis is a common and endemic disease that affects both humans and animals. In this stud... more Echinococcosis is a common and endemic disease that affects both humans and animals. In this study, the in vitro activities of methanolic extracts of Ruta graveolens, Peganum harmala aerial parts, and Citrullus colocynthis seeds against protoscolosis and isolated bacterial strains from hydatid cysts were assessed using disc diffusion methos and Minimum Inhibitory Concentration (MIC). The chemical composition of aerial sections of R. graveolensand P. harmala, as well as methanolic extracts of C. colocynthis seeds, was studied using LC-MS. These plants produced a total of 26, 31, and 23 chemicals, respectively. The bacteria listed below were isolated from hydatid cyst fluid collected from a variety of sick locations, including the lung and liver. Micrococcus spp., E. coli, Klebsiella oxytoca, Enterobacter aerogenes, Enterobacter amnigenus, Pseudomonas aeruginosa, Staphylococcus xylosus, and Achromobacter xylosoxidans are among the bacteria that have been identified. The most effective...
Research journal of pharmacy and technology, Dec 28, 2021
Alcea setosa A. (Malvaceae) is a wild plant that grows in Jordan and have several traditional med... more Alcea setosa A. (Malvaceae) is a wild plant that grows in Jordan and have several traditional medicinal uses. This study aims to collect and chemically analyze the methanolic extract from Alcea setosa A. from Jordan and to evaluate its cytotoxic and antioxidant activity against human breast cancer cells (T47D), colorectal adenocarcinoma cells (CACO2), and normal human fibroblasts (MRC5). The extract was extracted by methanol solvent and analyzed by liquid chromatography coupled with a mass spectrometer. Cell viability was assessed using trypan blue, neutral red, and MTT assays, and antioxidant activity was evaluated using DPPH scavenging activity assay. A total of 290 compounds, 12 among which were identified when compared to available standards, the extract contained six flavons derivatives, Two fatty acids, one ketone derivative, one flavonol derivative, one organic acid, and one coumarin derivative. The results also revealed that the IC50 values of the viability assays were higher among normal cells compared to the human cancer cell lines, and the viability inhibition was significant at higher concentrations compared to untreated cells. Nevertheless, moderate antioxidant activity was observed for the extract in the DPPH scavenging activity test. To sum up, this study indicates that samples of A. setosa collected from Jordan is likely to be an effective antioxidant, is optimistically potential to be utilized in breast and colon cancers treatment due to its preferential cytotoxicity against cancer cells.
Research journal of pharmacy and technology, Jan 25, 2022
Being multipurpose, relatively safe, and widely favorable for consumption, interest in the essent... more Being multipurpose, relatively safe, and widely favorable for consumption, interest in the essential oils of medicinal plants has been increasing. Essential oil (EO) of Juniperus phoenicea is traditionally used for treatment of several health problems such as diabetes, rheumatism, and cancer that is the second leading cause of death in Jordan. This study aims to collect and chemically analyze EO from Juniperus phoenicea L., from Jordan and to evaluate its cytotoxic and antioxidant activity against human breast cancer cells (T47D), colorectal adenocarcinoma cells (CACO2), and normal human fibroblasts (MRC5). EO was extracted by hydro-distillation and analyzed in a gas chromatograph coupled with a mass spectrometer. Cell viability was assessed using trypan blue, neutral red, and MTT assays, and antioxidant activity was evaluated using DPPH scavenging activity assay. Chemical composition analysis revealed 23 constituents in the EO, and the amount of α- pinene was the highest (69.71%). The results also revealed that the IC50 values of the viability assays were higher among normal cells compared to the human cancer cell lines, and the viability inhibition was significant at higher concentrations compared to untreated cells. Nevertheless, low antioxidant activity was observed for the oil in the DPPH scavenging activity test. To sum up, this study indicates that Jordanian Juniperus phoenicea EO, albeit unlikely to be an effective antioxidant, is optimistically potential to be utilized in breast and colon cancers treatment due to its preferential cytotoxicity against cancer cells.
Research journal of pharmacy and technology, Jan 25, 2022
The diversity in Jordan’s flora due to its geographical areas make is well noted in the scientifi... more The diversity in Jordan’s flora due to its geographical areas make is well noted in the scientific literature. The challenge of disease and death caused by infectious diseases like viruses and bacteria, and as infectious diseases evolve and pathogens develop resistance to existing pharmaceuticals, the search for new novel leads, possibly with different modes of action, against bacterial and viral diseases has intensified in recent years. The intent of this review is to provide prevalent information on the antibacterial and antiviral potential in medicinal plants in Jordan, mode of action, type of viruses and bacteria, and phytochemical contents. It has been demonstrated by several studies presented in this review that medicinal plants in Jordan are rich in phytochemicals and possess antiviral and antibacterial properties.
Context: Recent scientific studies have reported that essential oils induce apoptosis in various ... more Context: Recent scientific studies have reported that essential oils induce apoptosis in various cancer cell types by interfering with intracellular signaling pathways. Aims: To evaluate the cytotoxicity, the apoptotic activity of essential oil (EO) of Ruta chalepensis L. against MCF-7 cell line. Methods: Cytotoxicity was determined using methyl thiazol tetrazolium assay. The apoptotic activity of EO was analyzed using annexin V-fluorescein isothiocyanate/propidium iodide binding flow cytometry. The cell morphology was inspected under an inverted microscope. DAPI staining assay was used for the morphological observation. Activation of caspases-3/7, -8, and-9 was assessed using a caspase assay kit. Results: Ruta chalepensis essential oil significantly inhibited the proliferation of MCF-7 cells at 72 h. Moreover, the results showed that cell death is associated with the apoptotic process, and the number of apoptotic cells was significantly increased in the groups treated with EO than ...
Context: Ajuga orientalis L. (Lamiaceae) is an aromatic herb used in traditional medicine in Jord... more Context: Ajuga orientalis L. (Lamiaceae) is an aromatic herb used in traditional medicine in Jordan and neighboring countries. Aims: To evaluate the cytotoxic potential of ethanolic and aqueous extracts from the aerial parts of A. orientalis against three cell lines MCF-7, Caco-2, and HDFa. In addition to assessing the total phenolic and flavonoid contents, antioxidant activity. Further to analyze the phytochemical constituents. Methods: The phytochemical analysis was performed using gas chromatography/mass spectrometry. The total phenolic (TPC) and flavonoid (TFC) contents were assessed using colorimetric methods. The antioxidant properties of both extracts were assessed using 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity and reducing power ability. Cytotoxicity was investigated using methyl thiazol tetrazolium (MTT) assay. Results: The most abundant compounds in the extract were 9-octadecenoic acid, methyl ester, (E)- (27.2%), hexadecanoic acid, methyl ester (12...
Background: One of the primary health concerns for diabetes individuals is wounds. The used drugs... more Background: One of the primary health concerns for diabetes individuals is wounds. The used drugs have several side effects, urging the need for new natural sources for therapeutics. Materials and methods: This study was designed to estimate the wound healing potential of the methanolic extract of Globularia arabica and Malva sylvestris leaves and Rhus coriaria fruits. plant extracts were orally administered to the rats to determine their effect on the wound-healing process. Results: Plant extracts significantly increased the contraction of the wound in non-diabetic and diabetic rats (P < 0.05) and increased the fibroblast's proliferation and migration resulting in a faster healing process. The plant extracts have no cytotoxic effects. The proliferation assay exhibited the lowest cell mortality after treatment with plant extract. Conclusion: These findings may indicate that the methanolic leaf extract of the above plants can be used as new therapeutics for wound healing in diabetic patients.
AimThis study assessed the effects of topical application of R. coriaria extract on the rate of w... more AimThis study assessed the effects of topical application of R. coriaria extract on the rate of wound closure. The rate of wound contraction was used to assess the wound healing efficacy of the R. coriaria fruit methanolic extract.MethodsUsing excision and burn model of wound repair in diabetic male Wistar rats. Also, hydroxyproline, collagen content, and proinflammatory and anti‐inflammatory cytokines levels were determined in this study.ResultsDuring the early wound healing phase, interleukin 6 (IL‐6) levels were found to be decreased by R. coriaria treatment and increased the level of interleukin 10 (IL‐10). Increased wound contraction augmented with hydroxyproline and collagen content, supporting the early wound healing exhibited by R. coriaria. The epithelialization, neovascularization and enhanced hydroxyproline and collagen expression were strongly associated with the healing pattern.ConclusionThis study indicating that R. coriaria methanolic fruit extract has a potent wound ...
Echinococcosis is a common and endemic disease that affects both humans and animals. In this stud... more Echinococcosis is a common and endemic disease that affects both humans and animals. In this study, the in vitro activities of methanolic extracts of Ruta graveolens, Peganum harmala aerial parts, and Citrullus colocynthis seeds against protoscolosis and isolated bacterial strains from hydatid cysts were assessed using disc diffusion methos and Minimum Inhibitory Concentration (MIC). The chemical composition of aerial sections of R. graveolensand P. harmala, as well as methanolic extracts of C. colocynthis seeds, was studied using LC-MS. These plants produced a total of 26, 31, and 23 chemicals, respectively. The bacteria listed below were isolated from hydatid cyst fluid collected from a variety of sick locations, including the lung and liver. Micrococcus spp., E. coli, Klebsiella oxytoca, Enterobacter aerogenes, Enterobacter amnigenus, Pseudomonas aeruginosa, Staphylococcus xylosus, and Achromobacter xylosoxidans are among the bacteria that have been identified. The most effective...
Uploads