Arman Tabassum Rapid, sensitive and economical liquid chromatographic methods have been developed... more Arman Tabassum Rapid, sensitive and economical liquid chromatographic methods have been developed for simultaneous quantitation of gemifloxacin and rosuvastatin in Active Pharmaceutical Ingredients (API), pharmaceutical formulations and in human serum. At first, method was developed with chromatographic system consisting methanol and water in the ratio of 90:10 as mobile phase (pH adjusted 3 with o-phosphoric acid) passing through stationary phase of prepacked Purospher Star ® C18 (5 μm, 25 × 0.46 cm) column at 1.0 mLmin -
An efficient, selective and cost-effective liquid chromatographic assay was developed and validat... more An efficient, selective and cost-effective liquid chromatographic assay was developed and validated for the simultaneous quantification of ciprofloxacin and rosuvastatin in Active Pharmaceutical Ingredients (API), pharmaceutical formulations and in human serum. The chromatographic system consisted of mobile phase methanol-water, 90:10 v/v at pH 3.0 adjusted with o-phosphoric acid, pumped at 1.0 mL/min through a prepacked Purospher Star C18 (5 µm, 25 × 0.46 cm) column and effluent was monitored at the isosbestic point (255 nm) as well as at the λmax of individual drugs (243 and 271 nm). The method was validated over a linear concentration range of 0.25-15 µg/mL for ciprofloxacin and 0.33-20 µg/mL for rosuvastatin (r(2) ≥ 0.999). The ranges of reliable response (limits of detection and quantitation) for ciprofloxacin were 3-15 and 9-45 ng/mL and 17-29 and 52-88 ng/mL, respectively, for rosuvastatin in all API, pharmaceutical formulations and human serum. Analytical recovery from human...
Rapid, efficient and accurate RP-HPLC-UV method for the simultaneous determination and quality co... more Rapid, efficient and accurate RP-HPLC-UV method for the simultaneous determination and quality control of active pharmaceutical ingredient (API), pharmaceutical formulations and human serum containing drugs as rosuvastatin together with metformin, glimepiride and gliquidone has been proposed. The chromatographic system comprised mobile phase of methanol:water 90:10 v/v; pH adjusted to 3.0 with o-phosphoric acid, at 1 ml/min through Prepacked Purospher Star C18 (5 μm, 25×0.46 cm) column with UV detection at isosbestic point 231 nm. The method showed good linearity in the range 0.25-25 μg/ml for metformin and 0.5-50 μg/ml for rosuvastatin, glimepiride and gliquidone with correlation co-efficient ≥ 0.998; (precision %RSD<2) for all drugs in API, formulations and human serum. The recovery of all drugs was 98.9-101.91% in API and formulations and 99.92-102.08% in human serum. The sensitivity of method increased when drugs were analyzed after programming the detector at their individual λmax where their LODs shifted down to 5, 3, 10 and 9 ng/ml from 10, 17, 15 and 14 ng/ml when calculated at their isosbestic point respectively at least concentration 0.125 μg/ml for metformin and 0.25 μg/ml for rosuvastatin, glimepiride and gliquidone with correlation co-efficient ≥ 0.998 in each case. The proposed drugs can be analyzed by this method for routine analysis and clinical studies with sensitivity at nanoscale with small sample volume.
Arman Tabassum Rapid, sensitive and economical liquid chromatographic methods have been developed... more Arman Tabassum Rapid, sensitive and economical liquid chromatographic methods have been developed for simultaneous quantitation of gemifloxacin and rosuvastatin in Active Pharmaceutical Ingredients (API), pharmaceutical formulations and in human serum. At first, method was developed with chromatographic system consisting methanol and water in the ratio of 90:10 as mobile phase (pH adjusted 3 with o-phosphoric acid) passing through stationary phase of prepacked Purospher Star ® C18 (5 μm, 25 × 0.46 cm) column at 1.0 mLmin -
An efficient, selective and cost-effective liquid chromatographic assay was developed and validat... more An efficient, selective and cost-effective liquid chromatographic assay was developed and validated for the simultaneous quantification of ciprofloxacin and rosuvastatin in Active Pharmaceutical Ingredients (API), pharmaceutical formulations and in human serum. The chromatographic system consisted of mobile phase methanol-water, 90:10 v/v at pH 3.0 adjusted with o-phosphoric acid, pumped at 1.0 mL/min through a prepacked Purospher Star C18 (5 µm, 25 × 0.46 cm) column and effluent was monitored at the isosbestic point (255 nm) as well as at the λmax of individual drugs (243 and 271 nm). The method was validated over a linear concentration range of 0.25-15 µg/mL for ciprofloxacin and 0.33-20 µg/mL for rosuvastatin (r(2) ≥ 0.999). The ranges of reliable response (limits of detection and quantitation) for ciprofloxacin were 3-15 and 9-45 ng/mL and 17-29 and 52-88 ng/mL, respectively, for rosuvastatin in all API, pharmaceutical formulations and human serum. Analytical recovery from human...
Rapid, efficient and accurate RP-HPLC-UV method for the simultaneous determination and quality co... more Rapid, efficient and accurate RP-HPLC-UV method for the simultaneous determination and quality control of active pharmaceutical ingredient (API), pharmaceutical formulations and human serum containing drugs as rosuvastatin together with metformin, glimepiride and gliquidone has been proposed. The chromatographic system comprised mobile phase of methanol:water 90:10 v/v; pH adjusted to 3.0 with o-phosphoric acid, at 1 ml/min through Prepacked Purospher Star C18 (5 μm, 25×0.46 cm) column with UV detection at isosbestic point 231 nm. The method showed good linearity in the range 0.25-25 μg/ml for metformin and 0.5-50 μg/ml for rosuvastatin, glimepiride and gliquidone with correlation co-efficient ≥ 0.998; (precision %RSD<2) for all drugs in API, formulations and human serum. The recovery of all drugs was 98.9-101.91% in API and formulations and 99.92-102.08% in human serum. The sensitivity of method increased when drugs were analyzed after programming the detector at their individual λmax where their LODs shifted down to 5, 3, 10 and 9 ng/ml from 10, 17, 15 and 14 ng/ml when calculated at their isosbestic point respectively at least concentration 0.125 μg/ml for metformin and 0.25 μg/ml for rosuvastatin, glimepiride and gliquidone with correlation co-efficient ≥ 0.998 in each case. The proposed drugs can be analyzed by this method for routine analysis and clinical studies with sensitivity at nanoscale with small sample volume.
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