Communications Faculty of Science University of Ankara Series C Biology Geological Engineering and Geophysical Engineering
Antioxidants are known to restrains various tissue damage caused by the administration of carbon ... more Antioxidants are known to restrains various tissue damage caused by the administration of carbon tetrachloride (CCl4). This study examined whether melatonin (MEL), a molecule known to have antioxidant properties, has a protective effect on the rat soleus muscle, where toxic damage is caused by the application of CCl4. In the study, eighteen albino-type male Wistar rats were used and divided into three groups as Control Group (group 1), CCl4 group (group 2) and CCl4 + MEL group (group 3). End of the 12 weeks, blood samples were taken as intracardiac from the rats under ketamine/rompun anesthesia, and the soleus muscles of the rats were removed. Tissue samples were subjected to routine preparation procedures for light microscopy. Sections 5 µm thick taken and stained with hematoxylin-eosin (HE) for histopathological examinations and Masson’s Trichrome stain for fibrosis formations. In conclusion, the CCl4 group displayed muscular hypertrophy, fiber orientation dysfunction and atrophy ...
Stem cell transplantation is one of the available treatments for leukemia, lymphoma, hereditary b... more Stem cell transplantation is one of the available treatments for leukemia, lymphoma, hereditary blood diseases and bone marrow failure. Bone marrow (BM), peripheral blood progenitor cells (PBPC), and cord blood (CB) are the predominant sources of stem cells. Recently a new type of stem cell with a pluripotent potential has been identified. These cells were named “very small embryonic like stem cells (VSELs)”. It is claimed that VSEL stem cells can be found in adult BM, peripheral blood (PB), CB and other body tissues. This study is designed to characterize and isolate VSEL stem cells from different human hematopoietic sources; CB, PB and apheresis material (PBPC). VSEL stem cells were isolated from MNC and erythrocyte layers for all materials by using centrifugation and ficoll gradient method. We determined embryonic markers by flow cytometry, immunofluorescence and western blotting methods. Results from western blotting and immunofluorescence show high level of NANOG and OCT4 protein expression in PB, apheresis material and CB. Immunofluorescence images showed cytoplasmic and nuclear presence of these proteins. Flow cytometry results exhibited a higher expression of VSELs markers on debris area than CD45- population and higher expression on CB than PB. As a result, these findings have shown that it is necessary to investigate the function of pluripotent stem cell markers in differentiated adult cells. We further conclude that erythrocyte lysis method had the highest cell recovery amount among erythrocyte lysis and ficoll gradient methods. Consequently, this study gives us new information and viewpoints about expression of pluripotent stem cell (PSC) markers in adult tissues.
Medicine Science | International Medical Journal, 2021
There is no regulatory mechanism for the removal of iron that accumulates in the body. Thalassemi... more There is no regulatory mechanism for the removal of iron that accumulates in the body. Thalassemia patients are most affected by iron overload. The method often used in the treatment of these patients is iron chelation therapy, which involves removing excess iron from the bloodstream, but it is insufficient. Nutritional supplements and herbal remedies are complementary tools that may help improve the health of an individual with iron load. Here, we tried to develop a method that affecting intestinal absorption in an animal model with clinoptilolite feeding to reduce the iron load in the circulation. 32 rats were divided into 4 groups as control, Cli, Iron, Cli+Iron. Iron and Cli+Iron groups received iron at a dose of 250 mg/kg/day for 10 days, and Cli and Cli+Iron groups were fed a diet with 50% clinoptilolite for one month. Histological preparation and Fe2+, Cu2+, Zn2+ measurements were done in all tissues. Consequently, clinoptilolite significantly lowered the iron level in the stomach. On the contrary, iron absorption was increased in the small intestine, but iron transportation to the blood was decreased by clinoptilolite. The iron levels of clinoptilolite groups (Cli and Cli+Iron) were reduced in the tissues of heart, lung, liver, kidney, and spleen because of the different level of iron necessities of each tissue compared to the group with iron overload. The clinoptilolite could preserve organs against iron toxicity by enhancing the absorption of iron in the small intestine but lowering the iron level in blood. Even though there was no detailed information regarding the mechanism of reduction iron overload by the clinoptilolite, serum and chyme could help to make some helpful inferences to elucidate the mechanism of iron chelation.
Kolon kanseri neredeyse her yıl bir milyondan fazla yeni tanıların ortaya koyulduğu ve dünyada en... more Kolon kanseri neredeyse her yıl bir milyondan fazla yeni tanıların ortaya koyulduğu ve dünyada en sık rastlanan üçüncü kanser türüdür. Her yaş grubunda görülebildiği gibi kalıtımsal kökenli değildir. Bu veriler göz önüne alındığında kanserin tedavisi için yeni ilaç kombinasyonlarının araştırılması önemli bir ihtiyaçtır. Bu çalışmada, anti-kanser aktivitesi bilinmeyen Syntrichia ruraliformis (Besch.) Mans., Hypnum andoi A.J.E.Sm, Platyhypnidium riparioides Dixon karayosunu türlerinden elde edilen ekstraktlarının HCT116 kolon kanser hücre hatlarına karşı etkileri değerlendirilmiştir. Karayosunu türlerinin etil alkol çözücüsünde elde edilen ekstrelerinden, DMSO ile stok solüsyonlar hazırlanarak, çeşitli konsantrasyonlarda sitotoksite test (MTT) deneyleri yapılmıştır. HCT116 serisinde bulunan tüm karayosunu türlerinin IC50 değerlerine göre belirlenen dozlar aynı şekilde mezenkimal kök hücrelere (MKH) uygulanmıştır. Ayrıca, Fourier transform kızılötesi (FTIR) spektroskopisi üç karayosunu...
Abstract Colorectal cancer is the third most common cancer worldwide. Cancer stem cells are known... more Abstract Colorectal cancer is the third most common cancer worldwide. Cancer stem cells are known to play an important role in relapse, and metastases of the disease after chemotherapy. Investigation of new drugs, and their combinations targeting these cells and thus eliminating cancer is one of the most urgent needs of today’s chemotherapy. The aim of the present study was to evaluate the effects of Bryophytes like Abietinella abietina (AA), Homolothecium sericeum (HS), Tortella tortuosa (TT), Syntrichia ruralis (SR), and Bryoerythrophyllum rubrum (BR) species extracted with ethyl alcohol on 5-fluorouracil(5-FU) resistant colorectal cancer cell lines (HCT116 and HT29). After extraction, stock solutions of bryophytes were prepared, and IC50 values were detected in drug-resistant cells obtained with 5-FU application. CD24+, CD44+/CD133+ surface markers and P‐glycoprotein (P-gp) mediated efflux were isolated from both 5-FU treated cells and analyzed using the flow cytometry. In all bryophyte-treated groups, the binding Rho123low (low Rho fluorescence) and Rhohigh (high Rho fluorescence) were sorted from 5-FU resistant HCT116, and HT-29 cells. All types of bryophytes were found cytotoxic. Bryophyte extract reduced the percentage of Rholow cells in cultures incubated with 5-FU. In summary, the implementation of these bryophytes might be regarded as an effective approach for treatment of colorectal cancer due to their cytotoxic effect that decreases the recurrence of the disease. Supplemental data for this article is available online at https://doi.org/10.1080/01635581.2021.1933098
Abstract We investigated the protective effect of vitamin D against liver damage caused by carbon... more Abstract We investigated the protective effect of vitamin D against liver damage caused by carbon tetrachloride (CCl4). Twenty-four male rats were divided into four equal groups: G1, untreated controls; G2, administered CCl4; G3, administered both CCl4 and vitamin D for 10 weeks; G4, administered CCl4 for 10 weeks and vitamin D for 12 weeks. At the end of experiment, intracardiac blood samples were taken and liver samples were removed. Hepatic damage due to CCl4 was assessed using biochemistry and histopathology. Glutathione (GSH) levels decreased, while malondialdehyde (MDA) levels increased in liver tissues of G2. Alanine transaminase (ALT), alkaline phosphatase (ALP), and gamma-glutamyl-transaminase (GGT) levels increased, while albumin (ALB) levels decreased. Hepatocyte degeneration, lobular disorder, sinusoid dilation, focal necrotic areas, hyperemia, and glycogen loss were observed. Hepatic fibrosis was observed around portal areas and central veins. Bridging fibrous septa were formed between portal veins. By immunohistochemistry, both matrix metalloproteinase-9 (MMP-9) and desmin reactivity were increased. All aspects of liver damage were at least partially prevented in rats treated with vitamin D. Vitamin D appears to act as an antioxidant and anti-fibrotic to protect the rat liver against damage.
Karbon tetraklorur (CCl4) in vivo/in vitro modellerde karaciger toksisitesi olusturmak icin yaygi... more Karbon tetraklorur (CCl4) in vivo/in vitro modellerde karaciger toksisitesi olusturmak icin yaygin olarak kullanilmaktadir. Son zamanlarda, insan normal ve kanser hucrelerinde melatonin ve D Vitamininin hucre proliferasyonu uzerine yararli etkileri bulunmustur. Bu arastirma CCl4 sitotoksisitesi olusturulan HepG2 ve Hep3B hepatoma hucre hatlarinda melatonin ve D Vitamininin antioksidan ve hucre koruyucu etkilerini degerlendirmek icin planlandi. Sitotoksisite degerlendirmesine gore HepG2 ve HEP3B hucrelerinde melatonin ve D Vitamininin hucre koruyucu rolu hucre canliligi, lipit peroksidasyonu, SOD ve GSH seviyeleri olculerek degerlendirildi. Zamana bagli en yuksek toksik degeri belirlemek icin CCl4’un farkli dozlari (%0,1, 0,2, 0,3, 0,4 v/v) HepG2 ve HEP3B hucrelerine eklendi. Ayni uygulamalar ekzojen olarak MEL, D Vitamini ve CCl4’le beraber verilen gruplarda da tekrar edildi. Hucre canliligi 2., 24. ve 48. saatlerde MTT olcumleri ile belirlendi. GSH, SOD ve MDA seviyeleri hucre lizatlarindan olculdu. Sonuc olarak, CCl4 uygulamasi suresince MEL ve D Vitamini verilmesi CCl4’un olusturdugu hucresel hasara karsi karaciger hucrelerini korumustur. CCl4 uygulanmis hucre gruplarinin her ikisinde de lipit peroksidasyonunda artis ve GSH’da azalma saptanmistir. MEL ve D Vitamini uygulanan gruplarda ise kontrol gruplarina benzer sonuclar elde edilmistir. Bu nedenle melatonin ve D Vitamini cesitli toksik hepatik hastaliklarda umut verici bir toropatik ajan olabilir. AbstractCarbon tetrachloride (CCl4) is widely used to induce liver toxicity in vitro/in vivo models. Recently, there was found the beneficial effects of melatonin and Vitamin D on cell proliferation in normal human and cancer cells. This experiment was planned to evaluate antioxidant and cytoprotective effects of melatonin and Vitamin D in CCl4 induced cytotoxicity in HepG2 and Hep3B hepatoma cell lines. Based on the cytotoxicity assay, melatonin and Vitamin D was evaluated for cytotoprotective potential against CCl4 induced toxicity in HepG2 and Hep3B liver cell lines by monitoring cell viability, lipid peroxidation, SOD and GSH levels. Different dosages of CCl4 (0.1, 0.2, 0.3 and 0.4% v/v) were applied to HepG2 and Hep3B cells in order to determine the most toxic dosage of it in a time dependent manner. The same experiments were repeated with exogenously applied MEL, Vitamin D with/without CCl4 treated groups. Cell viability was determined with MTT measurements at 2nd,, 24th and 48th hours. GSH, SOD content and MDA level were measured from the cell lysates. As a result, both melatonin and Vitamin D administration during CCl4 exposure protected liver cells from CCl4 induced cellular damage. Increase in lipid peroxidation and decrease in GSH were found in both of the CCl4 groups of cells. Contrary to these results administration of MEL and Vitamin D on cells exhibited results similar to the control groups. Therefore, melatonin and Vitamin D might be a promising therapeutic agent in several toxic hepatic diseases.
ABSTRACT We investigated the distribution of connective tissue fibers in diabetic and vitamin C t... more ABSTRACT We investigated the distribution of connective tissue fibers in diabetic and vitamin C treated diabetic rat spleen. Rats were divided into three groups: group A, control; group B, diabetic; group C, vitamin C treated diabetic. Diabetes was induced by streptozotocin. Vitamin C was administered intragastrically for 21 days. Spleen tissues were examined by light microscopy after staining with Masson’s trichrome, Gomori silver impregnation and van Gieson. In group B, we found accumulation of collagen fibers in the trabeculae, in the capsule and around the central artery and splenic sinusoids. Splenic cord thickening due to fibrosis was observed. Reticular fibers accumulated principally in the white and red pulps of the spleen and focal reticular fiber thickening was observed in the dense fiber areas. Partial elastic fiber rupture was observed among the fibers of the elastic lamina of the arteries in the hilum. By contrast, the distribution of collagen fibers in group C was similar to group A. Collagen fiber accumulation was decreased in group C compared to group B. We found little reticular fiber thickening in group C and elastic fibers maintained their integrity and were better organized than in group B. Our findings suggest that appropriate doses of vitamin C may exert beneficial effects on the structure of the connective tissue fibers in the diabetic spleen.
Communications Faculty of Science University of Ankara Series C Biology Geological Engineering and Geophysical Engineering
Antioxidants are known to restrains various tissue damage caused by the administration of carbon ... more Antioxidants are known to restrains various tissue damage caused by the administration of carbon tetrachloride (CCl4). This study examined whether melatonin (MEL), a molecule known to have antioxidant properties, has a protective effect on the rat soleus muscle, where toxic damage is caused by the application of CCl4. In the study, eighteen albino-type male Wistar rats were used and divided into three groups as Control Group (group 1), CCl4 group (group 2) and CCl4 + MEL group (group 3). End of the 12 weeks, blood samples were taken as intracardiac from the rats under ketamine/rompun anesthesia, and the soleus muscles of the rats were removed. Tissue samples were subjected to routine preparation procedures for light microscopy. Sections 5 µm thick taken and stained with hematoxylin-eosin (HE) for histopathological examinations and Masson’s Trichrome stain for fibrosis formations. In conclusion, the CCl4 group displayed muscular hypertrophy, fiber orientation dysfunction and atrophy ...
Stem cell transplantation is one of the available treatments for leukemia, lymphoma, hereditary b... more Stem cell transplantation is one of the available treatments for leukemia, lymphoma, hereditary blood diseases and bone marrow failure. Bone marrow (BM), peripheral blood progenitor cells (PBPC), and cord blood (CB) are the predominant sources of stem cells. Recently a new type of stem cell with a pluripotent potential has been identified. These cells were named “very small embryonic like stem cells (VSELs)”. It is claimed that VSEL stem cells can be found in adult BM, peripheral blood (PB), CB and other body tissues. This study is designed to characterize and isolate VSEL stem cells from different human hematopoietic sources; CB, PB and apheresis material (PBPC). VSEL stem cells were isolated from MNC and erythrocyte layers for all materials by using centrifugation and ficoll gradient method. We determined embryonic markers by flow cytometry, immunofluorescence and western blotting methods. Results from western blotting and immunofluorescence show high level of NANOG and OCT4 protein expression in PB, apheresis material and CB. Immunofluorescence images showed cytoplasmic and nuclear presence of these proteins. Flow cytometry results exhibited a higher expression of VSELs markers on debris area than CD45- population and higher expression on CB than PB. As a result, these findings have shown that it is necessary to investigate the function of pluripotent stem cell markers in differentiated adult cells. We further conclude that erythrocyte lysis method had the highest cell recovery amount among erythrocyte lysis and ficoll gradient methods. Consequently, this study gives us new information and viewpoints about expression of pluripotent stem cell (PSC) markers in adult tissues.
Medicine Science | International Medical Journal, 2021
There is no regulatory mechanism for the removal of iron that accumulates in the body. Thalassemi... more There is no regulatory mechanism for the removal of iron that accumulates in the body. Thalassemia patients are most affected by iron overload. The method often used in the treatment of these patients is iron chelation therapy, which involves removing excess iron from the bloodstream, but it is insufficient. Nutritional supplements and herbal remedies are complementary tools that may help improve the health of an individual with iron load. Here, we tried to develop a method that affecting intestinal absorption in an animal model with clinoptilolite feeding to reduce the iron load in the circulation. 32 rats were divided into 4 groups as control, Cli, Iron, Cli+Iron. Iron and Cli+Iron groups received iron at a dose of 250 mg/kg/day for 10 days, and Cli and Cli+Iron groups were fed a diet with 50% clinoptilolite for one month. Histological preparation and Fe2+, Cu2+, Zn2+ measurements were done in all tissues. Consequently, clinoptilolite significantly lowered the iron level in the stomach. On the contrary, iron absorption was increased in the small intestine, but iron transportation to the blood was decreased by clinoptilolite. The iron levels of clinoptilolite groups (Cli and Cli+Iron) were reduced in the tissues of heart, lung, liver, kidney, and spleen because of the different level of iron necessities of each tissue compared to the group with iron overload. The clinoptilolite could preserve organs against iron toxicity by enhancing the absorption of iron in the small intestine but lowering the iron level in blood. Even though there was no detailed information regarding the mechanism of reduction iron overload by the clinoptilolite, serum and chyme could help to make some helpful inferences to elucidate the mechanism of iron chelation.
Kolon kanseri neredeyse her yıl bir milyondan fazla yeni tanıların ortaya koyulduğu ve dünyada en... more Kolon kanseri neredeyse her yıl bir milyondan fazla yeni tanıların ortaya koyulduğu ve dünyada en sık rastlanan üçüncü kanser türüdür. Her yaş grubunda görülebildiği gibi kalıtımsal kökenli değildir. Bu veriler göz önüne alındığında kanserin tedavisi için yeni ilaç kombinasyonlarının araştırılması önemli bir ihtiyaçtır. Bu çalışmada, anti-kanser aktivitesi bilinmeyen Syntrichia ruraliformis (Besch.) Mans., Hypnum andoi A.J.E.Sm, Platyhypnidium riparioides Dixon karayosunu türlerinden elde edilen ekstraktlarının HCT116 kolon kanser hücre hatlarına karşı etkileri değerlendirilmiştir. Karayosunu türlerinin etil alkol çözücüsünde elde edilen ekstrelerinden, DMSO ile stok solüsyonlar hazırlanarak, çeşitli konsantrasyonlarda sitotoksite test (MTT) deneyleri yapılmıştır. HCT116 serisinde bulunan tüm karayosunu türlerinin IC50 değerlerine göre belirlenen dozlar aynı şekilde mezenkimal kök hücrelere (MKH) uygulanmıştır. Ayrıca, Fourier transform kızılötesi (FTIR) spektroskopisi üç karayosunu...
Abstract Colorectal cancer is the third most common cancer worldwide. Cancer stem cells are known... more Abstract Colorectal cancer is the third most common cancer worldwide. Cancer stem cells are known to play an important role in relapse, and metastases of the disease after chemotherapy. Investigation of new drugs, and their combinations targeting these cells and thus eliminating cancer is one of the most urgent needs of today’s chemotherapy. The aim of the present study was to evaluate the effects of Bryophytes like Abietinella abietina (AA), Homolothecium sericeum (HS), Tortella tortuosa (TT), Syntrichia ruralis (SR), and Bryoerythrophyllum rubrum (BR) species extracted with ethyl alcohol on 5-fluorouracil(5-FU) resistant colorectal cancer cell lines (HCT116 and HT29). After extraction, stock solutions of bryophytes were prepared, and IC50 values were detected in drug-resistant cells obtained with 5-FU application. CD24+, CD44+/CD133+ surface markers and P‐glycoprotein (P-gp) mediated efflux were isolated from both 5-FU treated cells and analyzed using the flow cytometry. In all bryophyte-treated groups, the binding Rho123low (low Rho fluorescence) and Rhohigh (high Rho fluorescence) were sorted from 5-FU resistant HCT116, and HT-29 cells. All types of bryophytes were found cytotoxic. Bryophyte extract reduced the percentage of Rholow cells in cultures incubated with 5-FU. In summary, the implementation of these bryophytes might be regarded as an effective approach for treatment of colorectal cancer due to their cytotoxic effect that decreases the recurrence of the disease. Supplemental data for this article is available online at https://doi.org/10.1080/01635581.2021.1933098
Abstract We investigated the protective effect of vitamin D against liver damage caused by carbon... more Abstract We investigated the protective effect of vitamin D against liver damage caused by carbon tetrachloride (CCl4). Twenty-four male rats were divided into four equal groups: G1, untreated controls; G2, administered CCl4; G3, administered both CCl4 and vitamin D for 10 weeks; G4, administered CCl4 for 10 weeks and vitamin D for 12 weeks. At the end of experiment, intracardiac blood samples were taken and liver samples were removed. Hepatic damage due to CCl4 was assessed using biochemistry and histopathology. Glutathione (GSH) levels decreased, while malondialdehyde (MDA) levels increased in liver tissues of G2. Alanine transaminase (ALT), alkaline phosphatase (ALP), and gamma-glutamyl-transaminase (GGT) levels increased, while albumin (ALB) levels decreased. Hepatocyte degeneration, lobular disorder, sinusoid dilation, focal necrotic areas, hyperemia, and glycogen loss were observed. Hepatic fibrosis was observed around portal areas and central veins. Bridging fibrous septa were formed between portal veins. By immunohistochemistry, both matrix metalloproteinase-9 (MMP-9) and desmin reactivity were increased. All aspects of liver damage were at least partially prevented in rats treated with vitamin D. Vitamin D appears to act as an antioxidant and anti-fibrotic to protect the rat liver against damage.
Karbon tetraklorur (CCl4) in vivo/in vitro modellerde karaciger toksisitesi olusturmak icin yaygi... more Karbon tetraklorur (CCl4) in vivo/in vitro modellerde karaciger toksisitesi olusturmak icin yaygin olarak kullanilmaktadir. Son zamanlarda, insan normal ve kanser hucrelerinde melatonin ve D Vitamininin hucre proliferasyonu uzerine yararli etkileri bulunmustur. Bu arastirma CCl4 sitotoksisitesi olusturulan HepG2 ve Hep3B hepatoma hucre hatlarinda melatonin ve D Vitamininin antioksidan ve hucre koruyucu etkilerini degerlendirmek icin planlandi. Sitotoksisite degerlendirmesine gore HepG2 ve HEP3B hucrelerinde melatonin ve D Vitamininin hucre koruyucu rolu hucre canliligi, lipit peroksidasyonu, SOD ve GSH seviyeleri olculerek degerlendirildi. Zamana bagli en yuksek toksik degeri belirlemek icin CCl4’un farkli dozlari (%0,1, 0,2, 0,3, 0,4 v/v) HepG2 ve HEP3B hucrelerine eklendi. Ayni uygulamalar ekzojen olarak MEL, D Vitamini ve CCl4’le beraber verilen gruplarda da tekrar edildi. Hucre canliligi 2., 24. ve 48. saatlerde MTT olcumleri ile belirlendi. GSH, SOD ve MDA seviyeleri hucre lizatlarindan olculdu. Sonuc olarak, CCl4 uygulamasi suresince MEL ve D Vitamini verilmesi CCl4’un olusturdugu hucresel hasara karsi karaciger hucrelerini korumustur. CCl4 uygulanmis hucre gruplarinin her ikisinde de lipit peroksidasyonunda artis ve GSH’da azalma saptanmistir. MEL ve D Vitamini uygulanan gruplarda ise kontrol gruplarina benzer sonuclar elde edilmistir. Bu nedenle melatonin ve D Vitamini cesitli toksik hepatik hastaliklarda umut verici bir toropatik ajan olabilir. AbstractCarbon tetrachloride (CCl4) is widely used to induce liver toxicity in vitro/in vivo models. Recently, there was found the beneficial effects of melatonin and Vitamin D on cell proliferation in normal human and cancer cells. This experiment was planned to evaluate antioxidant and cytoprotective effects of melatonin and Vitamin D in CCl4 induced cytotoxicity in HepG2 and Hep3B hepatoma cell lines. Based on the cytotoxicity assay, melatonin and Vitamin D was evaluated for cytotoprotective potential against CCl4 induced toxicity in HepG2 and Hep3B liver cell lines by monitoring cell viability, lipid peroxidation, SOD and GSH levels. Different dosages of CCl4 (0.1, 0.2, 0.3 and 0.4% v/v) were applied to HepG2 and Hep3B cells in order to determine the most toxic dosage of it in a time dependent manner. The same experiments were repeated with exogenously applied MEL, Vitamin D with/without CCl4 treated groups. Cell viability was determined with MTT measurements at 2nd,, 24th and 48th hours. GSH, SOD content and MDA level were measured from the cell lysates. As a result, both melatonin and Vitamin D administration during CCl4 exposure protected liver cells from CCl4 induced cellular damage. Increase in lipid peroxidation and decrease in GSH were found in both of the CCl4 groups of cells. Contrary to these results administration of MEL and Vitamin D on cells exhibited results similar to the control groups. Therefore, melatonin and Vitamin D might be a promising therapeutic agent in several toxic hepatic diseases.
ABSTRACT We investigated the distribution of connective tissue fibers in diabetic and vitamin C t... more ABSTRACT We investigated the distribution of connective tissue fibers in diabetic and vitamin C treated diabetic rat spleen. Rats were divided into three groups: group A, control; group B, diabetic; group C, vitamin C treated diabetic. Diabetes was induced by streptozotocin. Vitamin C was administered intragastrically for 21 days. Spleen tissues were examined by light microscopy after staining with Masson’s trichrome, Gomori silver impregnation and van Gieson. In group B, we found accumulation of collagen fibers in the trabeculae, in the capsule and around the central artery and splenic sinusoids. Splenic cord thickening due to fibrosis was observed. Reticular fibers accumulated principally in the white and red pulps of the spleen and focal reticular fiber thickening was observed in the dense fiber areas. Partial elastic fiber rupture was observed among the fibers of the elastic lamina of the arteries in the hilum. By contrast, the distribution of collagen fibers in group C was similar to group A. Collagen fiber accumulation was decreased in group C compared to group B. We found little reticular fiber thickening in group C and elastic fibers maintained their integrity and were better organized than in group B. Our findings suggest that appropriate doses of vitamin C may exert beneficial effects on the structure of the connective tissue fibers in the diabetic spleen.
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