Investigation of the relationship between Antibiotic resistance pattern and class 1, 2 , 3 Integrons in Acinetobacter baumannii isolated from clinical samples of ICU patients, 2021
It can be seen that Cefepime showed the highest antibiotic resistance (100%) while Tigecycline (n... more It can be seen that Cefepime showed the highest antibiotic resistance (100%) while Tigecycline (no resistance) and Colistin (8.51%) showed the lowest antibiotic resistance. The frequency of class 1(82.98%), 2(36.17%) and 3(no frequency) Integrons was determined. Conclusion: We came to this conclusion that Acinetobacter baumannii had a high level of antibiotic resistance. There was a significant relationship between the presence of class 1, 2 and 3 Integrons and antibiotic resistance of the isolates to some kinds of antibiotics ABSTRACT: Acinetobacter baumannii, an opportunistic pathogen with high antibiotic resistance, can cause various infections, especially among hospitalized patients in burn and surgery intensive care units. This study is to investigate the relationship between Antibiotic resistance pattern and class 1, 2 and 3 Integrons in Acinetobacter baumannii isolated from clinical samples of ICU patients of Dr. Mohammad Kermanshahi Hospital in 2019. Methods: This study was done on 47 Acinetobacter baumannii isolates. Initial identification of the isolates was performed through phenotypic methods and final confirmation based on blaOXA-51 using PCR method. Antibiotic susceptibility of the isolates was determined by Disk-diffusion standard methods. The presence of class 1, 2 and 3 Integrons was investigated
To gain a better understanding of transmission and selecting appropriate measures for preventing ... more To gain a better understanding of transmission and selecting appropriate measures for preventing the spread of Helicobacter pylori, the aim of this study was to investigate the prevalence of H. pylori in drinking water samples in Kermanshah, Iran. Drinking water samples were collected from around Kermanshah and filtered through 0.45 μm nitrocellulose filters. The bacterial sediment was subjected to DNA extraction and polymerase chain reaction (PCR) for H. pylori detection using newly designed primers targeted at the conserved region of the ureC gene. The overall detection rates for H. pylori DNA in the water samples were 56% (66/118) with a frequency of 36% (25/70) in tap water samples and 85% (41/48) in wells. The detection limit was 50 bacteria per liter of filtered water and a pure H. pylori DNA copy number of 6 per reaction. Based on the evidence we may suggest that recontamination occurred and H. pylori entered into the water piping system through cracked or broken pipes or was...
Background and objective: A lot of people every day to do their banking transactions from
Autom... more Background and objective: A lot of people every day to do their banking transactions from
Automated Teller Machine. In case of contamination Automated Teller Machine keyboard as the
center of the action pollution and contamination transferred to future users. This study aimed to
investigate bacterial contamination Automated Teller Machine keyboard was conducted in the city
of Islamabad gharb.
Materials and methods: In this cross-sectional study, 53 Samples of the Automated Teller
Machine keyboard of Islamabad Gharb city were taken and moved to the lab. In the laboratory,
using standard diagnostic methods of bacteriology, including staining, biochemical tests and culture
in differential, selective and specific media were isolated of gram positive and negative bacteria.
Results: Based on the results all 53 samples were contaminated by bacteria. The most frequent
bacteria isolated Escherichia coli (37.72 percent) and lowest frequencies of Enterobacter (1.89
percent) and Diphtheroids (1.89 percent). The antibiotic resistant strains of Staphylococcus aureus
isolates, respectively the highest and lowest resistance was to penicillin 92.8 percent and
vancomycin 9.44 percent. The number of bacterial genera isolated keyboard appearance and
cleanliness there was a significant relationship (p< 0.05).
Conclusion: Due to contamination by pathogenic bacteria samples, education of personal hygiene
in members, Regular cleaning and disinfection devices and the use of antibacterial materials in the
structure of the keyboard in order to control and reduce pollution is effective.
Keywords: ATM systems, bacterial contamination, antibiotic resistance.
Investigation of the relationship between Antibiotic resistance pattern and class 1, 2 , 3 Integrons in Acinetobacter baumannii isolated from clinical samples of ICU patients, 2021
It can be seen that Cefepime showed the highest antibiotic resistance (100%) while Tigecycline (n... more It can be seen that Cefepime showed the highest antibiotic resistance (100%) while Tigecycline (no resistance) and Colistin (8.51%) showed the lowest antibiotic resistance. The frequency of class 1(82.98%), 2(36.17%) and 3(no frequency) Integrons was determined. Conclusion: We came to this conclusion that Acinetobacter baumannii had a high level of antibiotic resistance. There was a significant relationship between the presence of class 1, 2 and 3 Integrons and antibiotic resistance of the isolates to some kinds of antibiotics ABSTRACT: Acinetobacter baumannii, an opportunistic pathogen with high antibiotic resistance, can cause various infections, especially among hospitalized patients in burn and surgery intensive care units. This study is to investigate the relationship between Antibiotic resistance pattern and class 1, 2 and 3 Integrons in Acinetobacter baumannii isolated from clinical samples of ICU patients of Dr. Mohammad Kermanshahi Hospital in 2019. Methods: This study was done on 47 Acinetobacter baumannii isolates. Initial identification of the isolates was performed through phenotypic methods and final confirmation based on blaOXA-51 using PCR method. Antibiotic susceptibility of the isolates was determined by Disk-diffusion standard methods. The presence of class 1, 2 and 3 Integrons was investigated
To gain a better understanding of transmission and selecting appropriate measures for preventing ... more To gain a better understanding of transmission and selecting appropriate measures for preventing the spread of Helicobacter pylori, the aim of this study was to investigate the prevalence of H. pylori in drinking water samples in Kermanshah, Iran. Drinking water samples were collected from around Kermanshah and filtered through 0.45 μm nitrocellulose filters. The bacterial sediment was subjected to DNA extraction and polymerase chain reaction (PCR) for H. pylori detection using newly designed primers targeted at the conserved region of the ureC gene. The overall detection rates for H. pylori DNA in the water samples were 56% (66/118) with a frequency of 36% (25/70) in tap water samples and 85% (41/48) in wells. The detection limit was 50 bacteria per liter of filtered water and a pure H. pylori DNA copy number of 6 per reaction. Based on the evidence we may suggest that recontamination occurred and H. pylori entered into the water piping system through cracked or broken pipes or was...
Background and objective: A lot of people every day to do their banking transactions from
Autom... more Background and objective: A lot of people every day to do their banking transactions from
Automated Teller Machine. In case of contamination Automated Teller Machine keyboard as the
center of the action pollution and contamination transferred to future users. This study aimed to
investigate bacterial contamination Automated Teller Machine keyboard was conducted in the city
of Islamabad gharb.
Materials and methods: In this cross-sectional study, 53 Samples of the Automated Teller
Machine keyboard of Islamabad Gharb city were taken and moved to the lab. In the laboratory,
using standard diagnostic methods of bacteriology, including staining, biochemical tests and culture
in differential, selective and specific media were isolated of gram positive and negative bacteria.
Results: Based on the results all 53 samples were contaminated by bacteria. The most frequent
bacteria isolated Escherichia coli (37.72 percent) and lowest frequencies of Enterobacter (1.89
percent) and Diphtheroids (1.89 percent). The antibiotic resistant strains of Staphylococcus aureus
isolates, respectively the highest and lowest resistance was to penicillin 92.8 percent and
vancomycin 9.44 percent. The number of bacterial genera isolated keyboard appearance and
cleanliness there was a significant relationship (p< 0.05).
Conclusion: Due to contamination by pathogenic bacteria samples, education of personal hygiene
in members, Regular cleaning and disinfection devices and the use of antibacterial materials in the
structure of the keyboard in order to control and reduce pollution is effective.
Keywords: ATM systems, bacterial contamination, antibiotic resistance.
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Automated Teller Machine. In case of contamination Automated Teller Machine keyboard as the
center of the action pollution and contamination transferred to future users. This study aimed to
investigate bacterial contamination Automated Teller Machine keyboard was conducted in the city
of Islamabad gharb.
Materials and methods: In this cross-sectional study, 53 Samples of the Automated Teller
Machine keyboard of Islamabad Gharb city were taken and moved to the lab. In the laboratory,
using standard diagnostic methods of bacteriology, including staining, biochemical tests and culture
in differential, selective and specific media were isolated of gram positive and negative bacteria.
Results: Based on the results all 53 samples were contaminated by bacteria. The most frequent
bacteria isolated Escherichia coli (37.72 percent) and lowest frequencies of Enterobacter (1.89
percent) and Diphtheroids (1.89 percent). The antibiotic resistant strains of Staphylococcus aureus
isolates, respectively the highest and lowest resistance was to penicillin 92.8 percent and
vancomycin 9.44 percent. The number of bacterial genera isolated keyboard appearance and
cleanliness there was a significant relationship (p< 0.05).
Conclusion: Due to contamination by pathogenic bacteria samples, education of personal hygiene
in members, Regular cleaning and disinfection devices and the use of antibacterial materials in the
structure of the keyboard in order to control and reduce pollution is effective.
Keywords: ATM systems, bacterial contamination, antibiotic resistance.
Automated Teller Machine. In case of contamination Automated Teller Machine keyboard as the
center of the action pollution and contamination transferred to future users. This study aimed to
investigate bacterial contamination Automated Teller Machine keyboard was conducted in the city
of Islamabad gharb.
Materials and methods: In this cross-sectional study, 53 Samples of the Automated Teller
Machine keyboard of Islamabad Gharb city were taken and moved to the lab. In the laboratory,
using standard diagnostic methods of bacteriology, including staining, biochemical tests and culture
in differential, selective and specific media were isolated of gram positive and negative bacteria.
Results: Based on the results all 53 samples were contaminated by bacteria. The most frequent
bacteria isolated Escherichia coli (37.72 percent) and lowest frequencies of Enterobacter (1.89
percent) and Diphtheroids (1.89 percent). The antibiotic resistant strains of Staphylococcus aureus
isolates, respectively the highest and lowest resistance was to penicillin 92.8 percent and
vancomycin 9.44 percent. The number of bacterial genera isolated keyboard appearance and
cleanliness there was a significant relationship (p< 0.05).
Conclusion: Due to contamination by pathogenic bacteria samples, education of personal hygiene
in members, Regular cleaning and disinfection devices and the use of antibacterial materials in the
structure of the keyboard in order to control and reduce pollution is effective.
Keywords: ATM systems, bacterial contamination, antibiotic resistance.