Studies on the quinic acid utilisation gene (qut) cluster in Aspergillus nidulans showed that the... more Studies on the quinic acid utilisation gene (qut) cluster in Aspergillus nidulans showed that the genes encoding transcriptional activator and repressor proteins evolved by co-opting duplicated copies of genes encoding metabolic enzymes. In order to test the hypothesis that this was a general route for the genesis of regulatory proteins, the origins of the major control protein mediating nitrogen metabolite repression (an example of inter-pathway regulation) and ethanol utilisation (an example of intra-pathway regulation) in filamentous fungi were sought. The regulatory proteins mediating nitrogen metabolite repression were deduced to have originated in a duplication of genes encoding the anthranilate synthase complex which is active in the shikimate pathway. The major protein regulating ethanol utilisation was deduced to have its origin in the fusion of duplicated genes encoding the aldehyde and alcohol dehydrogenases (ALDA and ALCA). These data strongly support the view that transcriptional regulatory proteins evolve by the recruitment of functional domains provided by metabolic enzymes.
Preface Index Mendelian Genetics Prokaryotic Genetic Systems The Physical Mapping of DNA Using De... more Preface Index Mendelian Genetics Prokaryotic Genetic Systems The Physical Mapping of DNA Using Deletion Mutants to Make a Genetic Map Mutation Analysis Human Genetics Advanced Linkage Analysis.
Canadian journal of genetics and cytology, Sep 1, 1970
In a pseudo-wild heterokaryon of Neurospora, mixed perithecia in a cross in which the heterokaryo... more In a pseudo-wild heterokaryon of Neurospora, mixed perithecia in a cross in which the heterokaryon is used as the protoperithecial parent may be common. In the present work, 4 out of 22 perithecia contained more than one female nuclear type.A somatic recombinant nucleus, resulting from nondisjunction at anaphase II followed by recombination prior to breakdown of the disomic nucleus has been identified. The recombination event occurred between pyr-1 and tryp-4 on linkage group IV.
Predicted amino acid sequences of the enzyme orotidine 5'-phosphate decarboxylase (EC 4.1... more Predicted amino acid sequences of the enzyme orotidine 5'-phosphate decarboxylase (EC 4.1.1.23) from eight different organisms are compared. The comparisons are made on the basis of primary structural differences, primary amino acid sequence, hydropathy profiles, and secondary structure predictions. The organisms compared are Mus musculus, Aspergillus nidulans, Neurospora crassa, Kluyveromyces lactis, Saccharomyces cerevisiae, Schizosaccharomyces pombe, Escherichia coli, and Salmonella typhimurium.
Orotidine 5'-monophosphate decarboxylase protein sequences from 14 fungi, 1 slime mold, 2... more Orotidine 5'-monophosphate decarboxylase protein sequences from 14 fungi, 1 slime mold, 2 mammals, and 3 bacteria are compared and aligned and shown to be homologous. Based on the optimal alignment of the fungal sequences, a phylogenetic tree is derived. Within the fungi, the fission yeast Schizosaccharomyces pombe shows a closer relationship to both basidiomycetes and phycomycetes than it does to orthodox ascomycetes (plectomycetes, pyrenomycetes, and budding yeasts). Intron conservation shows a close relationship between phycomycetes and basidiomycetes. The imperfect fungi Trichoderma and Cephalosporium are shown to be closely related to Neurospora. The predicted origin of the group of budding yeasts is dependent on the analytical method used.
In studies on cellulase production by the cell-1 mutant of Neurospora crassa, eight enzymes (thre... more In studies on cellulase production by the cell-1 mutant of Neurospora crassa, eight enzymes (three exoglucanases, four endoglucanases, and one beta-glucosidase) were identified and characterized by gel filtration, ion exchange chromatography, and chromatofocusing. After purification, each of the proteins ran as a single band in polyacrylamide gel electrophoresis, using both native and denaturing gels. The molecular weights of the proteins were found to be between 70,000 and 22,000 daltons, and all were glycosylated, with carbohydrate contents ranging between 5.6% and 36%.
Studies on the quinic acid utilisation gene (qut) cluster in Aspergillus nidulans showed that the... more Studies on the quinic acid utilisation gene (qut) cluster in Aspergillus nidulans showed that the genes encoding transcriptional activator and repressor proteins evolved by co-opting duplicated copies of genes encoding metabolic enzymes. In order to test the hypothesis that this was a general route for the genesis of regulatory proteins, the origins of the major control protein mediating nitrogen metabolite repression (an example of inter-pathway regulation) and ethanol utilisation (an example of intra-pathway regulation) in filamentous fungi were sought. The regulatory proteins mediating nitrogen metabolite repression were deduced to have originated in a duplication of genes encoding the anthranilate synthase complex which is active in the shikimate pathway. The major protein regulating ethanol utilisation was deduced to have its origin in the fusion of duplicated genes encoding the aldehyde and alcohol dehydrogenases (ALDA and ALCA). These data strongly support the view that transcriptional regulatory proteins evolve by the recruitment of functional domains provided by metabolic enzymes.
Preface Index Mendelian Genetics Prokaryotic Genetic Systems The Physical Mapping of DNA Using De... more Preface Index Mendelian Genetics Prokaryotic Genetic Systems The Physical Mapping of DNA Using Deletion Mutants to Make a Genetic Map Mutation Analysis Human Genetics Advanced Linkage Analysis.
Canadian journal of genetics and cytology, Sep 1, 1970
In a pseudo-wild heterokaryon of Neurospora, mixed perithecia in a cross in which the heterokaryo... more In a pseudo-wild heterokaryon of Neurospora, mixed perithecia in a cross in which the heterokaryon is used as the protoperithecial parent may be common. In the present work, 4 out of 22 perithecia contained more than one female nuclear type.A somatic recombinant nucleus, resulting from nondisjunction at anaphase II followed by recombination prior to breakdown of the disomic nucleus has been identified. The recombination event occurred between pyr-1 and tryp-4 on linkage group IV.
Predicted amino acid sequences of the enzyme orotidine 5'-phosphate decarboxylase (EC 4.1... more Predicted amino acid sequences of the enzyme orotidine 5'-phosphate decarboxylase (EC 4.1.1.23) from eight different organisms are compared. The comparisons are made on the basis of primary structural differences, primary amino acid sequence, hydropathy profiles, and secondary structure predictions. The organisms compared are Mus musculus, Aspergillus nidulans, Neurospora crassa, Kluyveromyces lactis, Saccharomyces cerevisiae, Schizosaccharomyces pombe, Escherichia coli, and Salmonella typhimurium.
Orotidine 5'-monophosphate decarboxylase protein sequences from 14 fungi, 1 slime mold, 2... more Orotidine 5'-monophosphate decarboxylase protein sequences from 14 fungi, 1 slime mold, 2 mammals, and 3 bacteria are compared and aligned and shown to be homologous. Based on the optimal alignment of the fungal sequences, a phylogenetic tree is derived. Within the fungi, the fission yeast Schizosaccharomyces pombe shows a closer relationship to both basidiomycetes and phycomycetes than it does to orthodox ascomycetes (plectomycetes, pyrenomycetes, and budding yeasts). Intron conservation shows a close relationship between phycomycetes and basidiomycetes. The imperfect fungi Trichoderma and Cephalosporium are shown to be closely related to Neurospora. The predicted origin of the group of budding yeasts is dependent on the analytical method used.
In studies on cellulase production by the cell-1 mutant of Neurospora crassa, eight enzymes (thre... more In studies on cellulase production by the cell-1 mutant of Neurospora crassa, eight enzymes (three exoglucanases, four endoglucanases, and one beta-glucosidase) were identified and characterized by gel filtration, ion exchange chromatography, and chromatofocusing. After purification, each of the proteins ran as a single band in polyacrylamide gel electrophoresis, using both native and denaturing gels. The molecular weights of the proteins were found to be between 70,000 and 22,000 daltons, and all were glycosylated, with carbohydrate contents ranging between 5.6% and 36%.
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