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Isolation and Identification of Plant Growth-Promoting Endophytes Bacteria Highly Effective in Suppressing Damping-off in Sugar Beet

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Abstract

Sugar beet is an important sugar producing cash crop in the world with high economic value, but the crop faces a great economic loss every year due to damping-off. The concept of interactions produced between plant endophytic bacteria and host plants as an important biological control are gaining importance. In the present study, a strain of sugar beet standoff antagonist was obtained through plate standoff isolation and purification. Morphological observation, comparison of physiological and biochemical characteristics, and 16S rRNA gene sequences analysis were conducted for the classification and identification of the bacterial strain. The strain sb-13 was identified as Bacillus altitudinis. Through potting experiments, the growth of sugar beet inoculated with and without sb-13 was compared to analyze the effect of the endophytic bacteria on the growth of sugar beet, and it was found that the bacterium has the properties of promoting plant growth such as solubilizing phosphorus and dissolving potassium and enhancing the ability of soil enzyme activity. The experimental results showed that the growth of sugar beet inoculated with sb-13 was significantly better than that of the control group, and its physiological and biochemical indicators were significantly improved. In the anti-blight experiment, it was found that the bacterium antagonist had the ability to possess cellulose degradation and pectinase, and the incidence rate of sugar beet damping-off inoculated with sb-13 was significantly lower than that of the control group. This study provides a new idea for the biological control of sugar beet damping-off.

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Data availability

The data that support the findings of this study are available from the corresponding author upon reasonable request.

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Funding

Science Foundation for Distinguished Young Scholars of Heilongjiang University, Initiation Fund for Postdoctoral Research in Heilongjiang Province, and China Agriculture Research System Fund, CARS-170209, Yuguang Wang, Basic research business fund for provincial higher education institutions in Heilongjiang Province, 2022-KYYWF-1037, Yuguang Wang, Introduction Project for High-end Foreign Experts, G2023011004L, Yuguang Wang.

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Authors and Affiliations

  1. National Sugar Crop Improvement Centre, College of Advanced Agriculture and Ecological Environment, Heilongjiang University, Harbin, 150080, Heilongjiang, China

    Bingchen Zou, Tai Li, Yonglong Liu, Zeyuan Cui, Lihua Yu, Gui Geng, Jiahui Liu, Yao Xu & Yuguang Wang

  2. Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education and Heilongjiang Provincial Key Laboratory of Ecological Restoration and Resource Utilization for Cold Region and College of Life Sciences, Heilongjiang University, Harbin, 150500, Heilongjiang, China

    Gui Geng & Yuguang Wang

  3. Heilongjiang Sugar beet Engineering Technology Research Center, College of Advanced Agriculture and Ecological Environment, Heilongjiang University, Harbin, 150080, China

    Gui Geng & Yuguang Wang

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  1. Bingchen Zou
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Correspondence to Lihua Yu or Yuguang Wang.

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Zou, B., Li, T., Liu, Y. et al. Isolation and Identification of Plant Growth-Promoting Endophytes Bacteria Highly Effective in Suppressing Damping-off in Sugar Beet. Sugar Tech (2025). https://doi.org/10.1007/s12355-025-01547-9

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