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Certification and Long-Term Stability Assessments of 5-Methyltetrahydrofolate in Spinach and Kimchi Cabbage Certified Reference Materials Using Tri-enzyme Method and Isotope Dilution Ultra-performance Liquid Chromatography/Tandem Mass Spectrometry

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Abstract

This study presents the certification of spinach and kimchi cabbage certified reference materials (CRMs), (KRISS CRM 108-05-010 and 108-05-011), for 5-Me-THF. The fresh spinach and kimchi cabbage process involved washing, freeze-drying, pulverizing, sieving, V-mixing, and bottling in 10-g portions. The 5-Me-THF in the CRMs was then extracted and analyzed using a tri-enzyme method and isotope dilution ultra-performance liquid chromatography/tandem mass spectrometry. The method validation demonstrated that the method was both repeatable and reproducible, with the recovery ranging from 96.5 to 107.2%. The stability monitoring was performed for 7 days at room temperature, 1 month at − 20 °C, and 13 months at − 70 °C, confirming the stability of the CRMs under most conditions. The certified values were (16.93 ± 0.73) mg/kg and (11.66 ± 0.25) mg/kg for spinach and kimchi cabbage CRMs, respectively. The homogeneity of the CRMs was 1.85% and 0.91% for spinach and cabbage CRMs, respectively. Additional long-term stability assessments were performed, and this suggested that the CRMs will remain valid for at least another 48 months at − 70 °C.

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Data Availability

No datasets were generated or analysed during the current study.

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Funding

This work was supported by the Korea Research Institute of Standards and Science under the projects “Establishment of Measurement Standards for Nutrient Analysis” (Grant No. KRISS-2018-GP2018-0007), “Establishment of Measurement Standards for the Amount of Substance” (Grant No. KRISS-2019-GP2019-0006), “Establishment of Measurement Standards for Chemistry and Radiation” (Grant No. KRISS-2020-GP2020-0003), “Establishment of measurement standards for Chemistry and Radiation” (Grant No. KRISS-2021-GP-2021-0003), and “Establishment of measurement standards for Chemistry and Radiation” (Grant No. KRISS-2023-GP2023-0006).

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Authors and Affiliations

  1. Division of Chemical and Material Metrology, Korea Research Institute of Standards and Science, Yuseong, Daejeon, 34113, Republic of Korea

    Seok-Won Hyung & Joonhee Lee

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  1. Seok-Won Hyung
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Contributions

Seok-Won Hyung: Conceptualization, Resources, Investigation, Validation, Formal analysis, Methodology, Data curation, Supervision, Writing—original draft, Writing—review & editing. Joonhee Lee: Resources, Validation.

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Correspondence to Seok-Won Hyung.

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Hyung, SW., Lee, J. Certification and Long-Term Stability Assessments of 5-Methyltetrahydrofolate in Spinach and Kimchi Cabbage Certified Reference Materials Using Tri-enzyme Method and Isotope Dilution Ultra-performance Liquid Chromatography/Tandem Mass Spectrometry. Chromatographia (2024). https://doi.org/10.1007/s10337-024-04369-1

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