Abstract
Restricted Access Media-Methacrylic Resin with the ability of protein exclusion was prepared by the free radical polymerization using methacrylic acid, ethylene glycol dimethacrylate and glycidyl methacrylate as monomer, cross-linker and comonomer, respectively. Protein exclusion ability of this material was studied. The results showed that the material was able to eliminate 93.6% of protein after bovine serum albumin solution was added and shaken for 220 min. Adsorption kinetics and adsorption isotherms of Lamotrigine on this material were investigated. The experimental data were the most suitable for the pseudo-second-order kinetics model and Freundlich isotherm model. Restricted Access Media-Methacrylic Resin was used as sorbent for Solid-Phase Extraction to extract Lamotrigine from Human Plasma. The method for the determination of Lamotrigine in human plasma by SPE followed by the HPLC–UV presented linear range from 1.0 to 66.6 µg/mL with correlation coefficient 0.9930 for Lamotrigine, assay precision with relative standard deviation value 6.24%, and assay accuracy 94.7–104.7% with relative standard deviation values 4.33–5.81%. SPEs filled with this material were used at least 7 times without any significant changes in their performance. Analytical validation parameters demonstrated that the method could be applied to the determination of Lamotrigine at the therapeutic plasma levels without other treatments.
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References
Goa KL, Ross SR, Chrisp P (1993) Drugs 46:152–176. https://doi.org/10.2165/00003495-199346010-00009
Messenheimer J, Mullens EL, Giorgi L, Young F (1998) Drug Saf 18:281–296. https://doi.org/10.2165/00002018-199818040-00004
Pellock JM (1997) J Child Neurol 12:S48–S52. https://doi.org/10.1177/0883073897012001111
Burgoa Calvo ME, Domínguez Renedo O, Arcos Martínez MJ (2007) Talanta 74:59–64. https://doi.org/10.1016/j.talanta.2007.05.026
Burgoa Calvo ME, Domínguez Renedo O, Arcos Martínez MJ (2005) Anal Chim Acta 549:74–80. https://doi.org/10.1016/j.aca.2005.06.010
Contin M, Balboni M, Callegati E, Candela C, Albani F, Riva R, Baruzzi A (2005) J Chromatogr B 828:113–117. https://doi.org/10.1016/j.jchromb.2005.09.009
Contin M, Mohaned S, Candela C, Albani F, Riva R, Baruzzi A (2010) J Chromatogr B 878:461–465. https://doi.org/10.1016/j.jchromb.2009.11.039
Saracino MA, Bugamelli F, Clnti M, Amore M, Raggi MA (2007) J Sep Sci 30:2249–2255. https://doi.org/10.1002/jssc.200700110
Vermeij TAC, Edelbroek PM (2007) J Chromatogr B 857:40–46. https://doi.org/10.1016/j.jchromb.2007.16.023
Yang H, Zhang DJ, Mei SH, Zhan ZG (2022) J Pharm Biomed Anal 220:115017. https://doi.org/10.1016/j.jpba.2022.115017
Feng S, Bridgewater B, Strickland EC, McIntire G (2020) J Anal Toxicol 44:688–696. https://doi.org/10.1093/jat/bkaa095
Bazrafshan E, Dadfarnia S, Shabani AMH, Afsharipour R (2022) Spectrochim Acta A Mol Biomol Spectrosc 267:120530. https://doi.org/10.1016/j.saa.2021.120530
Mohajeri SA, Ebrahimi SH (2008) J Sep Sci 31:3595–3602. https://doi.org/10.1002/jssc.200800377
Greiner C, Haen E (2007) J Chromatogr B 854:338–344. https://doi.org/10.1016/j.jchromb.2007.04.006
Haginaka J, Takehira H, Hosoya K, Tanaka N (1999) J Chromatogr A 849:331–338. https://doi.org/10.1016/S0021-9673(99)00570-1
Sadílek P, Šatínský D, Solich P (2007) Trends Anal Chem 26:375–384. https://doi.org/10.1016/j.trac.2007.02.002
Song X, Zhang JL, Qiu W (2014) China Pharm 6:533–536. https://doi.org/10.6039/j.issn.1001-0408.2014.06.19
Parisi OI, Cirillo G, Curcio M, Puoci F, Iemma F, Spizzirri UG, Picci N (2010) J Polym Res 17:355–363. https://doi.org/10.1007/s10965-009-9322-7
de Faria HD, de Carvalho Abrão LC, Santos MG, Barbosa AF, Figueiredo EC (2017) Anal Chim Acta 959:43–65. https://doi.org/10.1016/j.aca.2016.12.047
Sanbe H, Haginaka J (2003) Analyst 128:593–597. https://doi.org/10.1039/B301257N
Ho YS, McKay G (1998) Chem Eng J 70:115–124. https://doi.org/10.1016/S0923-0467(98)00076-1
Morris GR, Black AB, Harris AL, Batty AB, Sallustio BC (1998) Br J Clin Pharmacol 46:647–551. https://doi.org/10.1046/j.1365-2125.1998.00835.x
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The work is funded by Cuiying Scientific and Technological Innovation Program of Lanzhou University Second Hospital (No. CY2020-MS14), Natural Science Foundation of Gansu province (No. 22JR5RA1007) and Cuiying Scientific Traning Program for Undergraduates of Lanzhou University Second Hospital (No. CYXZ2022-58)
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Xiaofei Wang and Qian Peng wrote the main manuscript text. Yumei Dong and Xiping Dou prepared all figures. Ping Li and Yinliang Bai prepared all tables. All authors reviewed the manuscript.
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Wang, X., Peng, Q., Dong, Y. et al. Preparation of Restricted Access Media-Methacrylic Resin and Its Application as the Sorbent of Solid-Phase Extraction in the Lamotrigine Determination in Human Plasma by HPLC–UV. Chromatographia (2024). https://doi.org/10.1007/s10337-024-04370-8
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DOI: https://doi.org/10.1007/s10337-024-04370-8