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Cu2O/SBA-3 Possess Intrinsic High Protease-Like Activity for Efficient Hydrolysis of Protein Under Physiological Conditions

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Abstract

Developing efficient artificial proteases still remains a great challenge due to the high stability of peptide bonds. Nanozymes have attracted great attention for their high stability, low cost and inherent physicochemical properties, providing new opportunities to break through natural enzyme inherent limitations, but the intrinsic mimic proteases properties of nanomaterials were seldom explored. Herein, we describe for the first time that SBA-3 supported Cu2O (Cu2O/SBA-3) exhibited excellent protease-like activity to hydrolysis of bovine serum albumin (BSA) and casein under neutral conditions, which is even superior to natural proteases (trypsin) and most of the other protease mimics under identical conditions. It exhibited surprisingly high catalytic activity and possessed good stability. As the first example of protease mimics consist of metallic compounds and mesoporous materials, Cu2O/SBA-3 has many advantages, such as easy preparation and separation, high activity and stability, mild reaction conditions, which makes this catalytic system have multiple of potential applications in biological systems.

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Acknowledgements

The authors thank the National Natural Science Foundation of China (22062026). The authors also thank the Yunling Scholar (K264202012420), Yunnan Applied Basic Research Projects (202401AT070432), Yunnan Fundamental Research Projects(202401BF070001-028), project from Department of Ecology and Environment of Yunnan Province (202305AM340008)，R & D Project (2022 No4) from Water Resources Department of Yunnan Province, project from Scientific and Technological Project of Yunnan Precious Metals Laboratory, Institute of Frontier Technologies in Water Treatment, and Key Laboratory of Advanced Materials for Wastewater Treatment of Kunming for financial support.

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Authors and Affiliations

  1. National Center for International Research On Photoelectric and Energy Materials, School of Materials and Energy, Yunnan University, Kunming, 650091, PR China

    Daomei Chen, Jian Tang, Sicong Li & Jiaqiang Wang

  2. School of Chemical Sciences & Technology, Yunnan University, Kunming, 650091, PR China

    Guo Xiao, Lixia zhao, Lingli Li, Tao Lei & Jiaqiang Wang

  3. Key Laboratory of Medicinal Chemistry for Natural Resource, Ministry of Education, Yunnan University, Kunming, 650091, PR China

    Bin Li & Jiaqiang Wang

  4. Institute of Frontier Technologies in Water, Treatment Co., Ltd., Kunming, 650503, PR China

    Jiaqiang Wang

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  1. Daomei Chen
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Chen, D., Xiao, G., Tang, J. et al. Cu2O/SBA-3 Possess Intrinsic High Protease-Like Activity for Efficient Hydrolysis of Protein Under Physiological Conditions. Catal Lett (2024). https://doi.org/10.1007/s10562-024-04747-2

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