Abstract
Fc receptors (FcRs), specific to the Fc portion of immunoglobulin (Ig), are required to regulate immune responses against pathogenic infections. However, FcγR is a member of FcRs family, whose structure and function remains to be elucidated in teleost fish. In this study, the FcγRII, from largemouth bass (Micropterus saloumoides), named membrane MsFcγRII (mMsFcγRII), was cloned and identified. The opening reading frame (ORF) of mMsFcγRII was 750 bp, encoding 249 amino acids with a predicted molecular mass of 27 kDa. The mMsFcγRII contained a signal peptide, two Ig domains, a transmembrane domain, and an intracellular region, which was highly homology with FcγR from other teleost fish. The mRNA expression analysis showed that mMsFcγRII was widely distributed in all tested tissues and with the highest expression level in spleen. After bacterial challenge, the expression of mMsFcγRII was significantly upregulated in vivo (spleen and head kidney), as well as in vitro (leukocytes from head kidney). The subcellular localization assay revealed that mMsFcγRII was mostly observed on the membrane of HEK293T cells which were transfected with mMsFcγRII overexpression plasmid. Flow cytometric analysis showed that natural mMsFcγRII protein was highly expressed in head kidney lymphocytes. Moreover, indirect immunofluorescence assay and pull-down assay indicated that mMsFcγRII could bind to IgM purified from largemouth bass serum. These results suggested that mMsFcγRII was likely to play an influential role in the immune response against pathogens and provided valuable insights for studying the function of FcRs in teleost.
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This research was supported by the Project of Collaborative Innovation Center of GDAAS (XT202305), the Natural Science Foundation of Guangdong Province, China (2022A1515012556), and the Special Financial Fund of Foshan-Guangdong Agricultural Science and the Technology Demonstration City Project in 2022.
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All authors contributed to the study conception and design. Jingya Wang performed the experiments, analyzed data, and drafted the manuscript. Jing Wu provided some experimental guidance. Yanping Ma and Le Hao provided some resources. Wen Huang revised the manuscript. Zhenxing Liu helped with experimental design and manuscript revision. Yugu Li designed this study and revised the manuscript. All authors read and approved the manuscript.
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Wang, J., Wu, J., Ma, Y. et al. Characterization of a membrane Fcγ receptor in largemouth bass (Micropterus saloumoides) and its response to bacterial challenge. Fish Physiol Biochem 50, 1123–1140 (2024). https://doi.org/10.1007/s10695-024-01325-6
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DOI: https://doi.org/10.1007/s10695-024-01325-6