Abstract
In this work, the corn straw (CS) with concentrations of 3%, 6%, and 9% (w/v) were pretreated by rumen fluid (RF) and then used for batched mesophilic biogas production. The results showed that after a 6-day pretreatment, volatile fatty acid (VFAs) production of 3.78, 8.27, and 10.4 g/L could be found in 3%, 6%, and 9%, respectively. When concerning with biogas production, the highest accumulative methane production of 149.1 mL CH4/g volatile solid was achieved by 6% pretreated CS, which was 22% and 45% higher than 3% and 9%, respectively. Also, it was 3.6 times higher than the same concentration of unpretreated CS. The results of the microbial community structure analysis revealed that the 6% CS pretreatment not only maintained a microbial community with the highest richness and diversity, but also exhibited the highest relative abundance of Firmicutes (45%) and Euryarchaeota (3.9%). This high abundance was conducive to its elevated production of VFAs and methane. These findings provide scientific reference for the utilization of CS and support the development of agricultural waste resource utilization and environmental protection.
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This work was supported by the National Key R&D Program of China (2021YFE0104600) and the Natural Science Foundation of Zhejiang Province (LY22E060002).
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All authors contributed to the study conception and design. Qing Yu has performed the experiments and prepared the first draft of the manuscript. The experimental guidance and manuscript modification were performed by Weixing Cao and Chen Sun. All authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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Yu, Q., Sun, C., Cao, W. et al. Rumen fluid pretreatment promotes anaerobic methane production: revealing microbial dynamics driving increased acid yield from different concentrations of corn straw. Environ Sci Pollut Res (2024). https://doi.org/10.1007/s11356-024-33615-0
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DOI: https://doi.org/10.1007/s11356-024-33615-0