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Imaging Inflammation by Intravital Microscopy

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Imaging Inflammation

Part of the book series: Progress in Inflammation Research ((PIR,volume 91))

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Abstract

The ability to visualise the biological processes involved in initiation and maintenance of inflammation is critical to our understanding of their context in pathophysiology. Intravital microscopy allows us to directly investigate leukocyte dynamics in vivo. Dynamic processes and effector functions can now synergistically be observed in live animal models and through indirect mechanistic experiments for a more complete picture. Thanks to intravital imaging, we now hold a detailed understanding of the leukocyte adhesion cascade, as well as mediators of effector cell migration and interactions with external pathogens. The combination of intravital microscopy and appropriate reporter models also allows the study of processes, such as sterile inflammation and aberrant modes of leukocyte trafficking, maintaining important tissue- and cell-specific detail. Importantly, intravital imaging also has the power to reveal behaviour, forcing us to refine long-held paradigms. From intravascular leukocyte functions to reverse transmigration and behaviour in non-blood fluids, intravital microscopy keeps pushing the boundaries of our understanding of inflammation. In this chapter, we give examples illustrating the role of intravital microscopy in describing canonical immunological processes, challenging established ideas and gathering insights into new paradigms by direct observation. We also discuss some of the technical challenges encountered during intravital imaging and how they are being overcome.

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Acknowledgements

MDD, FF and LMC are grateful for the help from Judith Secklehner and John Mackey along with the light microscopy (FILM and BAIR) and animal units (CBS and BSU) at Imperial College London and the CRUK Beatson Institute with the imaging examples used in Fig. 1.

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Authors and Affiliations

  1. Cancer Research UK Beatson Institute, Glasgow, UK

    Marco De Donatis, Frédéric Fercoq & Leo M. Carlin

  2. School of Cancer Sciences, University of Glasgow, Glasgow, UK

    Marco De Donatis & Leo M. Carlin

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  1. Marco De Donatis
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  2. Frédéric Fercoq
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  3. Leo M. Carlin
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Correspondence to Leo M. Carlin .

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Editors and Affiliations

  1. Institute of Pharmaceutical Science, King#x2019;s College London, London, UK

    Francis Man

  2. Department of Medicine, University of California, San Francisco, San Francisco, CA, USA

    Simon J. Cleary

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Funding: MDD, FF and LMC have received funding from Cancer Research UK (CRUK C596/A17196, A31287 and A23983) and Breast Cancer Now (2019DecPR1424).

Conflict of Interest: The authors have no conflicts of interest to declare.

Ethical approval: All experiments involving mice were performed in accordance with the UK Animal (Scientific Procedures) Act 1986, approved by the local animal welfare (AWERB) committees and conducted under UK Home Office licences.

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De Donatis, M., Fercoq, F., Carlin, L.M. (2023). Imaging Inflammation by Intravital Microscopy. In: Man, F., Cleary, S.J. (eds) Imaging Inflammation. Progress in Inflammation Research, vol 91. Springer, Cham. https://doi.org/10.1007/978-3-031-23661-7_7

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