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Inheritance and mapping of powdery mildew resistance gene Pm43 introgressed from Thinopyrum intermedium into wheat

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Abstract

Powdery mildew resistance from Thinopyrum intermedium was introgressed into common wheat (Triticum aestivum L.). Genetic analysis of the F1, F2, F3 and BC1 populations from powdery mildew resistant line CH5025 revealed that resistance was controlled by a single dominant allele. The gene responsible for powdery mildew resistance was mapped by the linkage analysis of a segregating F2 population. The resistance gene was linked to five co-dominant genomic SSR markers (Xcfd233, Xwmc41, Xbarc11, Xgwm539 and Xwmc175) and their most likely order was Xcfd233–Xwmc41–Pm43–Xbarc11–Xgwm539–Xwmc175 at 2.6, 2.3, 4.2, 3.5 and 7.0 cM, respectively. Using the Chinese Spring nullisomic-tetrasomic and ditelosomic lines, the polymorphic markers and the resistance gene were assigned to chromosome 2DL. As no powdery mildew resistance gene was previously assigned to chromosome 2DL, this new resistance gene was designated Pm43. Pm43, together with the identified closely linked markers, could be useful in marker-assisted selection for pyramiding powdery mildew resistance genes.

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Acknowledgments

The authors are grateful to Drs. Bernd Friebe, Chengdao Li and Robert McIntosh for critical reviews of this manuscript, and to Dr. Shubing Liu for technical guidance in the SSR analyses. This project was funded by National Natural Science Foundation (30671299 and 39870398) and Shanxi Key Technologies R and D Program of China.

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Authors and Affiliations

  1. School of Life Science and Technology, Shanxi University, 030006, Taiyuan, Shanxi, China

    Runli He, Zhijian Chang & Jianxia Liu

  2. Institute of Crop Genetics, Shanxi Academy of Agricultural Sciences, 030031, Taiyuan, Shanxi, China

    Zhijian Chang, Haixian Zhan & Xiaojun Zhang

  3. School of Life Science and Technology, University of Electronic Science and Technology of China, 610054, Chengdu, Sichuan, China

    Zujun Yang

  4. Institute of Plant Protection, Shanxi Academy of Agricultural Sciences, 030031, Taiyuan, Shanxi, China

    Zongying Yuan

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  1. Runli He
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Correspondence to Zhijian Chang.

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Communicated by E. Guiderdoni.

Runli He and Zhijian Chang contributed equally to this work.

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He, R., Chang, Z., Yang, Z. et al. Inheritance and mapping of powdery mildew resistance gene Pm43 introgressed from Thinopyrum intermedium into wheat. Theor Appl Genet 118, 1173–1180 (2009). https://doi.org/10.1007/s00122-009-0971-z

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