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Design of mineral medium for growth of Actinomadura sp. ATCC 39727, producer of the glycopeptide A40926: effects of calcium ions and nitrogen sources

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Abstract

Actinomadura sp. ATCC 39727 produces the glycopeptide antibiotic A40926, structurally similar to teicoplanin, with significant activity against Neisseria gonorrhoeae and precursor of the semi-synthetic antibiotic dalbavancin. In this study the production of A40926 by Actinomadura under a variety of growth conditions was investigated. The use of chemically defined mineral media allowed us to analyze the influence of carbon and nitrogen sources, phosphate, ammonium and calcium on the growth and the antibiotic productivity of Actinomadura. We confirm recent data [Gunnarsson et al. (2003) J Ind Microbiol Biotechnol 30:150–156] that low initial concentrations of phosphate and ammonium are beneficial for growth and A40926 production, and we provide new evidence that the production of A40926 is depressed by calcium, but promoted when l-glutamine or l-asparagine are used as nitrogen sources instead of ammonium salts.

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Acknowledgements

This work was supported as part of the Progetto MIUR 488 “Nuove tecnologie per lo studio di microrganismi altoproduttori di antibiotici” coordinated by Aventis Bulk S.p.A., Italy. The authors are grateful to Prof. Sergio Papa (Università degli Studi di Lecce) for his continuous advice and support of the present work, to Dr. Raffaele Palumbo (Aventis Bulk S.p.A., Brindisi, Italy) for helpful discussions, and to Miss Rita Bennett for English editing of the manuscript.

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Authors and Affiliations

  1. Institute of Microbiology, Czech Academy of Science, Prague, Czech Republic

    Zuzana Technikova-Dobrova

  2. Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Università degli Studi di Lecce, Via Monteroni, 73100, Lecce, Italy

    Fabrizio Damiano, Salvatore M. Tredici, Giovanni Vigliotta, Roberta di Summa, Gabriele V. Gnoni & Pietro Alifano

  3. Dipartimento di Biochimica Medica e Biologia Medica, Università degli Studi di Bari, Plazza G. Cesare-Policlinico, 70124, Bari, Italy

    Luigi Palese, Anna Abbrescia & Nestor Labonia

  4. Universidad de Morón, Buenos Aires, Argentina

    Nestor Labonia

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  1. Zuzana Technikova-Dobrova
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  2. Fabrizio Damiano
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  3. Salvatore M. Tredici
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  10. Pietro Alifano
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Correspondence to Pietro Alifano.

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Technikova-Dobrova, Z., Damiano, F., Tredici, S.M. et al. Design of mineral medium for growth of Actinomadura sp. ATCC 39727, producer of the glycopeptide A40926: effects of calcium ions and nitrogen sources. Appl Microbiol Biotechnol 65, 671–677 (2004). https://doi.org/10.1007/s00253-004-1626-2

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  • DOI: https://doi.org/10.1007/s00253-004-1626-2

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