Abstract
Nonomuraea sp. ATCC 39727 is a novel actinomycete species and the producer of A40926, a glycopeptide antibiotic structurally similar to teichoplanin. In the present study, a defined minimal medium was designed for Nonomuraea fermentation. The influence of initial phosphate, glucose and ammonium concentrations on antibiotic productivity was investigated in batch fermentation and the effect of glucose limitation was studied in fed-batch fermentation. It was found that low initial concentrations of phosphate and ammonium are beneficial for A40926 production and that productivity is not enhanced during glucose limitation. Furthermore, the initiation of A40926 production was not governed by residual ammonium and phosphate concentrations, although the level of these nutrients strongly influenced A40926 production rates and final titers.
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References
Bhatnagar RK, Doull JL, Vining LC (1988) Role of the carbon source in regulating chloramphenicol production by Streptomyces venezuelae: studies in batch and continuous cultures. Can J Microbiol 34:1217–1233
Bonten MJ, Willems R, Weinstein RA (2001) Vancomycin-resistant enterococci: why are they here, and where do they come from? Lancet Infect Dis 1:314–325
Chatterjee S, Vining LC, Westlake DWS (1983) Nutritional requirements for chloramphenical biosynthesis in Streptomyces venezuelae. Can J Microbiol 29:247–253
Doull JL, Vining LC (1990) Nutritional control of actinorhodin production by Streptomyces coelicolor A3(2): suppressive effects of nitrogen and phosphate. Appl Microbiol Biotechnol 32:449–454
Farr BM, Salgado CD, Karchmer TB, Sherertz RJ (2001) Can antibiotic-resistant nosocomial infections be controlled? Lancet Infect Dis 1:38–45
Goldstein BP, Selva E, Gastaldo L, Berti M, Pallanza R, Ripamonti F, Ferrari P, Denaro M, Arioli V, Cassani G (1987) A40926, a new glycopeptide antibiotic with anti-Neisseria activity. Antimicrob Agents Chemother 31:1961–1966
Heydorn A, Suhr-Jessen T, Nielsen J (1999) Growth and production kinetics of a teichoplanin producing strain of Actinoplanes teichomyceticus. J Antibiot 52:40–44
Jonsbu E, Ellingsen TE Nielsen J (2000) Effects of nitrogen sources on cell growth and production of nystatin by Streptomyces noursei. J Antibiot 53:1354–1362
Malabarba A, Ciabatti R, Scott R, Goldstein BP, Ferrari P, Kurz M, Andreini BP, Denaro M (1995) New semisynthetic glycopeptides MDL 63,246 and MDL 63,042, and other amide derivatives of antibiotic A-40,926 active against highly glycopeptide-resistant VanA enterococci. J Antibiot 48:869–883
Martin JF, Demain AL (1980) Control of antibiotic synthesis. Microbiol Rev 44:230–251
McIntyre JJ, Bull AT, Bunch AW (1996) Vancomycin production in batch and continuous culture. Biotechnol Bioeng 49:412–420
Riva E, Zanol M, Selva E, Borghi A (1987) Column purification and HPLC determination of teicoplanin and A40926. Chromatographia 24:295–301
Steiert M, Schmitz FJ (2002) Dalbavancin (Biosearch Italia/Versicor). Curr Opin Invest Drugs 3:229–233
Strahilevitz J, Rubenstein E (2002) Novel agents for resistant Gram-positive infections—a review. Int J Infect Dis 6:38–46
Vara AG, Hochkoepple A, Nielsen J, Villadsen J (2002) Production of teicoplanin by Actinoplanes teichomyceticus in continuous fermentation. Biotechnol Bioeng 77:589–598
Zerilli LF, Edwards DM, Borghi A, Gallo GG, Selva E, Denaro M, Lancini GC (1992) Determination of the acyl moieties of the antibiotic complex A40926 and their relation with the membrane lipids of the producer strain. Rapid Commun Mass Spectrom 6:109–114
Acknowledgements
We thank Flavia Marinelli and Srdjan Jovetic, Biosearch Italia SpA, for advice on the handling of the Nonomuraea strain and Einar Jonsbu for providing helpful comments on medium design for actinomycetes. This work was financed by the European Union via the MEGA-TOP project QLK3-1999-00650; and we acknowledge the scientific discussions with our colleagues participating in this project.
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Gunnarsson, N., Bruheim, P. & Nielsen, J. Production of the glycopeptide antibiotic A40926 by Nonomuraea sp. ATCC 39727: influence of medium composition in batch fermentation. J IND MICROBIOL BIOTECHNOL 30, 150–156 (2003). https://doi.org/10.1007/s10295-003-0024-6
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DOI: https://doi.org/10.1007/s10295-003-0024-6