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Introgression of UfCyt c6, a thylakoid lumen protein from a green seaweed Ulva fasciata Delile enhanced photosynthesis and growth in tobacco

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Abstract

Cytochromes are important components of photosynthetic electron transport chain. Here we report on genetic transformation of Cytochrome c6 (UfCyt c6) gene from Ulva fasciata Delile in tobacco for enhanced photosynthesis and growth. UfCyt c6 cDNA had an open reading frame of 330 bp encoding a polypeptide of 109 amino acids with a predicted molecular mass of 11.65 kDa and an isoelectric point of 5.21. UfCyt c6 gene along with a tobacco petE transit peptide sequence under control of CaMV35S promoter was transformed in tobacco through Agrobacterium mediated genetic transformation. Transgenic tobacco grew normal and exhibited enhanced growth as compared to wild type (WT) and vector control (VC) tobacco. Transgenic tobacco had higher contents of photosynthetic pigments and better ratios of photosynthetic pigments. The tobacco expressing UfCyt c6 gene exhibited higher photosynthetic rate and improved water use efficiency. Further activity of the water-splitting complex, photosystem II quantum yield, photochemical quenching, electron transfer rate, and photosynthetic yield were found comparatively higher in transgenic tobacco as compared to WT and VC tobacco. Alternatively basal quantum yield of non-photochemical processes in PSII and non-photochemical quenching were estimated lower in tobacco expressing UfCyt c6 gene. As a result of improved photosynthetic performance the transgenic tobacco had higher contents of sugar and starch, and exhibited comparatively better growth. To the best of our knowledge this is the first report on expression of UfCyt c6 gene from U. fasciata for improved photosynthesis and growth in tobacco.

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Acknowledgements

CSIR-CSMCRI PRIS 159/2017. Authors thankfully acknowledge the CSIR-Central Salt and Marine Chemicals Research Institute, Govt. of India for financial support (CSC0116) under XIIth five year plan project. KK acknowledge financial support from CSIR, Govt. of India in the form of CSIR-JRF. SY and KK are thankful to Academy of Council of Scientific and Industrial Research (AcSIR), New Delhi for registration in Ph.D. program.

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  1. Academy of Scientific and Innovative Research, CSIR, New Delhi, India

    Sweta K. Yadav, Kusum Khatri, Mangal S. Rathore & Bhavanath Jha

  2. Division of Biotechnology and Phycology, CSIR-Central Salt and Marine Chemicals Research Institute (CSIR-CSMCRI), Council of Scientific and Industrial Research (CSIR), G.B. Marg, Bhavnagar, Gujarat, 364002, India

    Mangal S. Rathore & Bhavanath Jha

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11033_2018_4318_MOESM1_ESM.jpg

In silico analysis: Nucleotide sequence of UfCyt c6 gene with derived amino acids sequence (a), predicted folding pattern of UfCyt c6 protein (b), predicated secondary and tertiary structure of UfCyt c6 protein (c – d), predicted localization of UfCyt c6 protein as a soluble lumen protein (e) and predicted phosphorylation sites in UfCyt c6 protein (f). The underline sequence represent UTR region and the asterisk denotes N glycosylation in amino acid sequence in the nucleotide / amino acid sequence. Supplementary figure S1 (JPG 58 KB)

11033_2018_4318_MOESM2_ESM.jpg

Visual growth observations of soil transplanted WC, VC and T0 generation transgenic plants under normal growth conditions in greenhouse after 30 (a), 75 (b), and 90 (c) days of growth—Supplementary figure S2 (JPG 494 KB)

11033_2018_4318_MOESM3_ESM.jpg

Comparison of leaf (3rd leaf) area after 6 weeks of growth in soil (a) and flowering (b) in WT, VC and transgenic lines (L1 and L3)—Supplementary figure S3 (JPG 524 KB)

11033_2018_4318_MOESM4_ESM.jpg

Chlorophyll a (a) and chlorophyll b (b) contents in WT, VC and transgenic lines (L1 and L3) after 15 d (a), 30 d (b), 45 d (c) and 60 d (d) growth in soil—Supplementary figure S4 (JPG 429 KB)

11033_2018_4318_MOESM5_ESM.jpg

F0 (a), FM (b), FV (c), FV / F0 (d), F0 / FM (e), FV’ / FM’ (f), 1-qP (g) and qN (h) in WT, VC and transgenic lines (L1 and L3) under field conditions after 45 days of soil transplantation—Supplementary figure S5 (JPG 556 KB)

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Yadav, S.K., Khatri, K., Rathore, M.S. et al. Introgression of UfCyt c6, a thylakoid lumen protein from a green seaweed Ulva fasciata Delile enhanced photosynthesis and growth in tobacco. Mol Biol Rep 45, 1745–1758 (2018). https://doi.org/10.1007/s11033-018-4318-1

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