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Fucoxanthin as a major carotenoid in Isochrysis aff. galbana: Characterization of extraction for commercial application

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Abstract

Fucoxanthin, a main marine carotenoid, in five species of fucoxanthin-containing microalgae, was quantified by high-performance liquid chromatography. Among the studied species, Isochrysis aff. galbana contained the highest amount of fucoxanthin (18.23 mg/g dried sample). This microalga showed good fucoxanthin extraction efficiency under the tested solvents (methanol, ethanol, acetone, and ethyl acetate), with the exception of n-hexane. In addition, most fucoxanthin (∼95%) could be extracted by a single extraction in ethanol within 5 min, and only 15% degradation of fucoxanthin was detected during ethanol extraction for 24 h. The two-phase solvent system of n-hexaneethanol-water with a volume ratio of 10:9:1 was determined to be the best system for the separation of fucoxanthin and lipids from extracts of I. aff. galbana. Under these conditions, fucoxanthin was fractionated in the hydroalcohol phase apart from the hexane phase containing lipids. These results imply that I. aff. galbana can be a commercial source for the spontaneous production of valuable fucoxanthins and lipids.

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Authors and Affiliations

  1. Functional Food Center, Korea Institute of Science and Technology, Gangneung, 210-340, Republic of Korea

    Sang Min Kim, Suk-Woo Kang & Cheol-Ho Pan

  2. Marine Biology Center for Research and Education, Gangneung-Wonju National University, Gangneung, 210-853, Republic of Korea

    O-Nam Kwon

  3. Department of Marine Food Science and Technology, Medical and Bio-Material Research Center, Gangneung-Wonju National University, Gangneung, 210-702, Republic of Korea

    Donghwa Chung

Authors
  1. Sang Min Kim
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  2. Suk-Woo Kang
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  3. O-Nam Kwon
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  4. Donghwa Chung
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  5. Cheol-Ho Pan
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Correspondence to Cheol-Ho Pan.

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Kim, S.M., Kang, SW., Kwon, ON. et al. Fucoxanthin as a major carotenoid in Isochrysis aff. galbana: Characterization of extraction for commercial application. J Korean Soc Appl Biol Chem 55, 477–483 (2012). https://doi.org/10.1007/s13765-012-2108-3

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  • DOI: https://doi.org/10.1007/s13765-012-2108-3

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