Quercus suber (cork oak) is an evergreen tree native to the Mediterranean basin, which plays a ke... more Quercus suber (cork oak) is an evergreen tree native to the Mediterranean basin, which plays a key role in the ecology and economy of this area. Over the last decades, this species has gone through an observable decline, mostly due to environmental factors. Deciphering the mechanisms of cork oak’s response to the environment and getting a deep insight into its biology are crucial to counteract biotic and abiotic stresses compromising the stability of a unique ecosystem. In the light of these setbacks, the publication of the genome in 2018 was a major step towards understanding the genetic make-up of this species. In an effort to integrate this information in a comprehensive, accessible and intuitive format, we have developed The Cork Oak Genome Database Portal (CorkOakDB). The CorkOakDB is supported by the BioData.pt e-infrastructure, the Portuguese ELIXIR node for biological data. The portal gives public access to search and explore the curated genomic and transcriptomic data on th...
Cork cambium (or phellogen) is a secondary meristem, responsible for the formation of phelloderm ... more Cork cambium (or phellogen) is a secondary meristem, responsible for the formation of phelloderm and phellem/cork, which together compose the periderm. In Quercus suber, the phellogen is active throughout the entire life of the tree, producing a continuous and renewable outer bark of cork. To identify specific candidate genes associated with cork cambium activity and phellem differentiation, we performed a comparative transcriptomic study of Q. suber secondary growth tissues (xylem and phellogen/phellem), using RNA-seq. The present work provides a high resolution map of all the transcripts identified in phellogen/phellem tissues. A total of 6,013 differentially expressed genes (DEGs) were identified, with 2,875 of the transcripts being specifically enriched during the cork formation process versus secondary xylem formation. Furthermore, cork samples originating from the original phellogen (“virgin” cork) and from a traumatic phellogen (“amadia” cork) were also compared. Our results ...
Addressing the risk of mixed carcinogens in vivo under environmentally-realistic scenarios is sti... more Addressing the risk of mixed carcinogens in vivo under environmentally-realistic scenarios is still a challenge. Searching for adequate biomarkers of exposure requires understanding molecular pathways and their connection with neoplasia-related benchmark pathologies. Subjecting the zebrafish model to realistic concentrations of two genotoxicants and carcinogens, cadmium and benzo[a]pyrene, isolated and combined, yielded low levels of DNA damage. Altogether, the organisms' mechanisms of DNA repair, oxidative stress and phases I and II were not overwhelmed after two weeks of treatment. Still, transcriptional responses related to detoxification (epoxide hydrolase and UDP-glucuronosyltransferase) were higher in animals subjected to the combination treatment, inclusively following depuration. Nonetheless, inflammation and formation of hyperplasic foci in fish epithelia were more severe in animals exposed to the combined substances, showing slower recovery during depuration. Additiona...
XXV International Horticultural Congress, Part 11: Application of Biotechnology and Molecular Biology and Breeding - Gene Expression and Molecular Breeding, Genome Analysis., 2000
... TESTING OF A TRANSFORMATION VECTOR AIMING AT INTRODUCING PDV RESISTANCE IN ALMOND Raquel, MH1... more ... TESTING OF A TRANSFORMATION VECTOR AIMING AT INTRODUCING PDV RESISTANCE IN ALMOND Raquel, MH1 and Miguel, C2. ... are transmitted by vegetative propagation (ie: grafting or micropropagation), mechanical inoculation, pollen grains and seeds (Fulton, 1983 ...
II International Symposium on Pistachios and Almonds, 1998
... 12 2780 Oeiras PORTUGAL Raquel MH Dep. ... production of almonds was around 25,000 tonnes, 80... more ... 12 2780 Oeiras PORTUGAL Raquel MH Dep. ... production of almonds was around 25,000 tonnes, 80-90 % being exported and creating an income of about 300 million РТЕ a year, a value corresponding to more than half of that obtained from all Portuguese fruits (Ferrão, 1982). ...
Embryogenic cultures were obtained from seeds of open-pollinated maritime pine trees representing... more Embryogenic cultures were obtained from seeds of open-pollinated maritime pine trees representing part of a breeding population. The aim of the present study was to develop and optimize a protocol for cryopreservation of Pinus pinaster somatic embryogenic tissue. The density of the embryogenic suspension and the type of pre-treatment significantly affected the recovery of cryopreserved embryogenic cultures, as evaluated by their survival and regrowth rate. An initial density of the suspension culture of 250 mg/ml improved the regrowth rate of the embryogenic lines. Pre-treatment with maltose 0.4 M significantly increased the regrowth rate when compared to the other tested carbohydrates. Also, the addition of DMSO in a mixture of PEG 4000 and sucrose (PSD solution), instead of DMSO alone, at the same final concentration, was clearly beneficial for recovery of cryopreserved tissues. This improved method for the cryopreservation of P pinaster embryogenic of cultures allowed the success...
Quercus suber (cork oak) is an evergreen tree native to the Mediterranean basin, which plays a ke... more Quercus suber (cork oak) is an evergreen tree native to the Mediterranean basin, which plays a key role in the ecology and economy of this area. Over the last decades, this species has gone through an observable decline, mostly due to environmental factors. Deciphering the mechanisms of cork oak’s response to the environment and getting a deep insight into its biology are crucial to counteract biotic and abiotic stresses compromising the stability of a unique ecosystem. In the light of these setbacks, the publication of the genome in 2018 was a major step towards understanding the genetic make-up of this species. In an effort to integrate this information in a comprehensive, accessible and intuitive format, we have developed The Cork Oak Genome Database Portal (CorkOakDB). The CorkOakDB is supported by the BioData.pt e-infrastructure, the Portuguese ELIXIR node for biological data. The portal gives public access to search and explore the curated genomic and transcriptomic data on th...
Cork cambium (or phellogen) is a secondary meristem, responsible for the formation of phelloderm ... more Cork cambium (or phellogen) is a secondary meristem, responsible for the formation of phelloderm and phellem/cork, which together compose the periderm. In Quercus suber, the phellogen is active throughout the entire life of the tree, producing a continuous and renewable outer bark of cork. To identify specific candidate genes associated with cork cambium activity and phellem differentiation, we performed a comparative transcriptomic study of Q. suber secondary growth tissues (xylem and phellogen/phellem), using RNA-seq. The present work provides a high resolution map of all the transcripts identified in phellogen/phellem tissues. A total of 6,013 differentially expressed genes (DEGs) were identified, with 2,875 of the transcripts being specifically enriched during the cork formation process versus secondary xylem formation. Furthermore, cork samples originating from the original phellogen (“virgin” cork) and from a traumatic phellogen (“amadia” cork) were also compared. Our results ...
Addressing the risk of mixed carcinogens in vivo under environmentally-realistic scenarios is sti... more Addressing the risk of mixed carcinogens in vivo under environmentally-realistic scenarios is still a challenge. Searching for adequate biomarkers of exposure requires understanding molecular pathways and their connection with neoplasia-related benchmark pathologies. Subjecting the zebrafish model to realistic concentrations of two genotoxicants and carcinogens, cadmium and benzo[a]pyrene, isolated and combined, yielded low levels of DNA damage. Altogether, the organisms' mechanisms of DNA repair, oxidative stress and phases I and II were not overwhelmed after two weeks of treatment. Still, transcriptional responses related to detoxification (epoxide hydrolase and UDP-glucuronosyltransferase) were higher in animals subjected to the combination treatment, inclusively following depuration. Nonetheless, inflammation and formation of hyperplasic foci in fish epithelia were more severe in animals exposed to the combined substances, showing slower recovery during depuration. Additiona...
XXV International Horticultural Congress, Part 11: Application of Biotechnology and Molecular Biology and Breeding - Gene Expression and Molecular Breeding, Genome Analysis., 2000
... TESTING OF A TRANSFORMATION VECTOR AIMING AT INTRODUCING PDV RESISTANCE IN ALMOND Raquel, MH1... more ... TESTING OF A TRANSFORMATION VECTOR AIMING AT INTRODUCING PDV RESISTANCE IN ALMOND Raquel, MH1 and Miguel, C2. ... are transmitted by vegetative propagation (ie: grafting or micropropagation), mechanical inoculation, pollen grains and seeds (Fulton, 1983 ...
II International Symposium on Pistachios and Almonds, 1998
... 12 2780 Oeiras PORTUGAL Raquel MH Dep. ... production of almonds was around 25,000 tonnes, 80... more ... 12 2780 Oeiras PORTUGAL Raquel MH Dep. ... production of almonds was around 25,000 tonnes, 80-90 % being exported and creating an income of about 300 million РТЕ a year, a value corresponding to more than half of that obtained from all Portuguese fruits (Ferrão, 1982). ...
Embryogenic cultures were obtained from seeds of open-pollinated maritime pine trees representing... more Embryogenic cultures were obtained from seeds of open-pollinated maritime pine trees representing part of a breeding population. The aim of the present study was to develop and optimize a protocol for cryopreservation of Pinus pinaster somatic embryogenic tissue. The density of the embryogenic suspension and the type of pre-treatment significantly affected the recovery of cryopreserved embryogenic cultures, as evaluated by their survival and regrowth rate. An initial density of the suspension culture of 250 mg/ml improved the regrowth rate of the embryogenic lines. Pre-treatment with maltose 0.4 M significantly increased the regrowth rate when compared to the other tested carbohydrates. Also, the addition of DMSO in a mixture of PEG 4000 and sucrose (PSD solution), instead of DMSO alone, at the same final concentration, was clearly beneficial for recovery of cryopreserved tissues. This improved method for the cryopreservation of P pinaster embryogenic of cultures allowed the success...
Uploads