Main pharmacokinetic parameters of three randomly-selected generic products and reference product... more Main pharmacokinetic parameters of three randomly-selected generic products and reference product of 14 immediate-release, non-combinational, oral drugs. (DOCX 40Â kb)
Log-concentration-time curves of a reference and three randomly-selected generic products of 14 i... more Log-concentration-time curves of a reference and three randomly-selected generic products of 14 immediate-release, non-combinational, oral drugs. Log-concentration-time curves of a reference and three randomly-selected generic products of 14 immediate-releases, non-combinational, oral drugs (a to n). Data represent mean log-transformed concentrations. Blue diamond indicates reference, red square generic a, green triangle generic b, and purple cross generic c. (PPTX 30562Â kb)
Authors‟ reply: We don‟t find this statement to be true. We benefited from all the recommendation... more Authors‟ reply: We don‟t find this statement to be true. We benefited from all the recommendations of reviewer #2, who stated in his re-evaluation: “I am satisfied that my original comments have been addressed adequately.” It was not possible for us to accept some of the recommendations of reviewer #1, such as [I think the term is "hypercalcurea."] and [D2 and D3 levels were not determined!]. However, we did accept several of reviewer #1 recommendations and we thanked him (and thank him again) for his intend to improve the manuscript.
Authors‟ reply: We don‟t find this statement to be true. We benefited from all the recommendation... more Authors‟ reply: We don‟t find this statement to be true. We benefited from all the recommendations of reviewer #2, who stated in his re-evaluation: “I am satisfied that my original comments have been addressed adequately.” It was not possible for us to accept some of the recommendations of reviewer #1, such as [I think the term is "hypercalcurea."] and [D2 and D3 levels were not determined!]. However, we did accept several of reviewer #1 recommendations and we thanked him (and thank him again) for his intend to improve the manuscript.
We developed and validated a sensitive, specific, a nd rapid HPLC assay for ketoprofen measuremen... more We developed and validated a sensitive, specific, a nd rapid HPLC assay for ketoprofen measurement in human plasma and studied its stability under various cond itions. After extraction with the tert butyl methyl ether, ketoprofen and diclofenac (internal standard) were eluted on 8 Nova-Pak C 18 , 4 µm cartridge at room temperature (RT) with a run time of 10 min. The mobile phase consisted of 0.2% glacial ace tic acid (pH 3.0) and acetonitrile (50:50, v:v). The analytes were detected using phot odiode array detector set at 258 nm. The response w as linear over the range of 0.02‐10 µg/ml. Extraction recover y of ketoprofen and inter-run bias and precision we re ≥ 90% (mean 96%), ≤ 2%, and ≤ 8.1%, respectively. Ketoprofen was stable in plasm a for 24 hours at RT (100%), 10 weeks at ‐20 0 C ( ≥ 97%), and after 3 cycles of freeze at ‐20 0 C and thaw at RT (98%). In extracted samples, ketoprofen was stable for 24 hours at RT ( ≥ 94%) and 48 hours at‐20 0 C (99%). Stock solution of ketopro...
Main pharmacokinetic parameters of three randomly-selected generic products and reference product... more Main pharmacokinetic parameters of three randomly-selected generic products and reference product of 14 immediate-release, non-combinational, oral drugs. (DOCX 40Â kb)
Log-concentration-time curves of a reference and three randomly-selected generic products of 14 i... more Log-concentration-time curves of a reference and three randomly-selected generic products of 14 immediate-release, non-combinational, oral drugs. Log-concentration-time curves of a reference and three randomly-selected generic products of 14 immediate-releases, non-combinational, oral drugs (a to n). Data represent mean log-transformed concentrations. Blue diamond indicates reference, red square generic a, green triangle generic b, and purple cross generic c. (PPTX 30562Â kb)
We developed and validated a sensitive, specific, a nd rapid HPLC assay for ketoprofen measuremen... more We developed and validated a sensitive, specific, a nd rapid HPLC assay for ketoprofen measurement in human plasma and studied its stability under various cond itions. After extraction with the tert butyl methyl ether, ketoprofen and diclofenac (internal standard) were eluted on 8 Nova-Pak C 18 , 4 µm cartridge at room temperature (RT) with a run time of 10 min. The mobile phase consisted of 0.2% glacial ace tic acid (pH 3.0) and acetonitrile (50:50, v:v). The analytes were detected using phot odiode array detector set at 258 nm. The response w as linear over the range of 0.02‐10 µg/ml. Extraction recover y of ketoprofen and inter-run bias and precision we re ≥ 90% (mean 96%), ≤ 2%, and ≤ 8.1%, respectively. Ketoprofen was stable in plasm a for 24 hours at RT (100%), 10 weeks at ‐20 0 C ( ≥ 97%), and after 3 cycles of freeze at ‐20 0 C and thaw at RT (98%). In extracted samples, ketoprofen was stable for 24 hours at RT ( ≥ 94%) and 48 hours at‐20 0 C (99%). Stock solution of ketopro...
We examined the specificity of the recently reported alterations in circulating lymphocytes in he... more We examined the specificity of the recently reported alterations in circulating lymphocytes in heatstroke by determining lymphocyte subsets in 14 consecutive heatstroke patients before and after cooling and in 7 heat-stressed controls using single- or two-color immunofluorescence flow cytometry. The relationship with catecholamine levels was also studied. In heatstroke, percentages of T (CD3+/CD19−), T-helper (CD4+/CD8−), T-inactive [CD3+/human leukocyte antigen-DR−], CD11a+, CD11c+, and CD44+ lymphocytes were significantly decreased, whereas percentages of T-suppressor-cytotoxic (CD8+/CD4−), natural killer (NK; CD3−/CD16+or CD56+), CD3+/CD16+or CD56+, and CD54+ lymphocytes were significantly increased, compared with 11 normal controls. The changes in the absolute numbers of lymphocyte subsets were in the same direction and were significant for T-helper, T-suppressor-cytotoxic, NK, CD3+/CD16+or CD56+, and CD11c+ lymphocytes. Milder but significant changes in percentages of T-helper,...
Main pharmacokinetic parameters of three randomly-selected generic products and reference product... more Main pharmacokinetic parameters of three randomly-selected generic products and reference product of 14 immediate-release, non-combinational, oral drugs. (DOCX 40Â kb)
Log-concentration-time curves of a reference and three randomly-selected generic products of 14 i... more Log-concentration-time curves of a reference and three randomly-selected generic products of 14 immediate-release, non-combinational, oral drugs. Log-concentration-time curves of a reference and three randomly-selected generic products of 14 immediate-releases, non-combinational, oral drugs (a to n). Data represent mean log-transformed concentrations. Blue diamond indicates reference, red square generic a, green triangle generic b, and purple cross generic c. (PPTX 30562Â kb)
Authors‟ reply: We don‟t find this statement to be true. We benefited from all the recommendation... more Authors‟ reply: We don‟t find this statement to be true. We benefited from all the recommendations of reviewer #2, who stated in his re-evaluation: “I am satisfied that my original comments have been addressed adequately.” It was not possible for us to accept some of the recommendations of reviewer #1, such as [I think the term is "hypercalcurea."] and [D2 and D3 levels were not determined!]. However, we did accept several of reviewer #1 recommendations and we thanked him (and thank him again) for his intend to improve the manuscript.
Authors‟ reply: We don‟t find this statement to be true. We benefited from all the recommendation... more Authors‟ reply: We don‟t find this statement to be true. We benefited from all the recommendations of reviewer #2, who stated in his re-evaluation: “I am satisfied that my original comments have been addressed adequately.” It was not possible for us to accept some of the recommendations of reviewer #1, such as [I think the term is "hypercalcurea."] and [D2 and D3 levels were not determined!]. However, we did accept several of reviewer #1 recommendations and we thanked him (and thank him again) for his intend to improve the manuscript.
We developed and validated a sensitive, specific, a nd rapid HPLC assay for ketoprofen measuremen... more We developed and validated a sensitive, specific, a nd rapid HPLC assay for ketoprofen measurement in human plasma and studied its stability under various cond itions. After extraction with the tert butyl methyl ether, ketoprofen and diclofenac (internal standard) were eluted on 8 Nova-Pak C 18 , 4 µm cartridge at room temperature (RT) with a run time of 10 min. The mobile phase consisted of 0.2% glacial ace tic acid (pH 3.0) and acetonitrile (50:50, v:v). The analytes were detected using phot odiode array detector set at 258 nm. The response w as linear over the range of 0.02‐10 µg/ml. Extraction recover y of ketoprofen and inter-run bias and precision we re ≥ 90% (mean 96%), ≤ 2%, and ≤ 8.1%, respectively. Ketoprofen was stable in plasm a for 24 hours at RT (100%), 10 weeks at ‐20 0 C ( ≥ 97%), and after 3 cycles of freeze at ‐20 0 C and thaw at RT (98%). In extracted samples, ketoprofen was stable for 24 hours at RT ( ≥ 94%) and 48 hours at‐20 0 C (99%). Stock solution of ketopro...
Main pharmacokinetic parameters of three randomly-selected generic products and reference product... more Main pharmacokinetic parameters of three randomly-selected generic products and reference product of 14 immediate-release, non-combinational, oral drugs. (DOCX 40Â kb)
Log-concentration-time curves of a reference and three randomly-selected generic products of 14 i... more Log-concentration-time curves of a reference and three randomly-selected generic products of 14 immediate-release, non-combinational, oral drugs. Log-concentration-time curves of a reference and three randomly-selected generic products of 14 immediate-releases, non-combinational, oral drugs (a to n). Data represent mean log-transformed concentrations. Blue diamond indicates reference, red square generic a, green triangle generic b, and purple cross generic c. (PPTX 30562Â kb)
We developed and validated a sensitive, specific, a nd rapid HPLC assay for ketoprofen measuremen... more We developed and validated a sensitive, specific, a nd rapid HPLC assay for ketoprofen measurement in human plasma and studied its stability under various cond itions. After extraction with the tert butyl methyl ether, ketoprofen and diclofenac (internal standard) were eluted on 8 Nova-Pak C 18 , 4 µm cartridge at room temperature (RT) with a run time of 10 min. The mobile phase consisted of 0.2% glacial ace tic acid (pH 3.0) and acetonitrile (50:50, v:v). The analytes were detected using phot odiode array detector set at 258 nm. The response w as linear over the range of 0.02‐10 µg/ml. Extraction recover y of ketoprofen and inter-run bias and precision we re ≥ 90% (mean 96%), ≤ 2%, and ≤ 8.1%, respectively. Ketoprofen was stable in plasm a for 24 hours at RT (100%), 10 weeks at ‐20 0 C ( ≥ 97%), and after 3 cycles of freeze at ‐20 0 C and thaw at RT (98%). In extracted samples, ketoprofen was stable for 24 hours at RT ( ≥ 94%) and 48 hours at‐20 0 C (99%). Stock solution of ketopro...
We examined the specificity of the recently reported alterations in circulating lymphocytes in he... more We examined the specificity of the recently reported alterations in circulating lymphocytes in heatstroke by determining lymphocyte subsets in 14 consecutive heatstroke patients before and after cooling and in 7 heat-stressed controls using single- or two-color immunofluorescence flow cytometry. The relationship with catecholamine levels was also studied. In heatstroke, percentages of T (CD3+/CD19−), T-helper (CD4+/CD8−), T-inactive [CD3+/human leukocyte antigen-DR−], CD11a+, CD11c+, and CD44+ lymphocytes were significantly decreased, whereas percentages of T-suppressor-cytotoxic (CD8+/CD4−), natural killer (NK; CD3−/CD16+or CD56+), CD3+/CD16+or CD56+, and CD54+ lymphocytes were significantly increased, compared with 11 normal controls. The changes in the absolute numbers of lymphocyte subsets were in the same direction and were significant for T-helper, T-suppressor-cytotoxic, NK, CD3+/CD16+or CD56+, and CD11c+ lymphocytes. Milder but significant changes in percentages of T-helper,...
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