Lumpy skin disease (LSD) is an economically devastating and transboundary disease in cattle. Here... more Lumpy skin disease (LSD) is an economically devastating and transboundary disease in cattle. Here, we report the coding-complete genome sequence of the LSDV72/PrachuapKhiriKhan/Thailand/2021 strain, which was isolated from an affected cow during the first LSD outbreak in Thailand in 2021. The sequence will be beneficial for future genomic studies of the virus.
Purpose To understand the complication and histopathological characteristics between the Silk Fib... more Purpose To understand the complication and histopathological characteristics between the Silk Fibroin/Polyurethanes (SF/PU) and the host response, and to unveil the compatibility of the patch in diabetes individuals. Methods Rats were divided into DM and control (CT) groups, and the DM group was induced with streptozotocin. All groups underwent the SF/PU patch implantation in the abdominal aorta, and the implanted patches were evaluated at one, two, three, and four weeks after implantation. Results DM group had more fibrosis formation and a delayed endothelialization compared to the CT group. There was no evidence of chronic inflammation in both DM and CT groups. Conclusions Fibrosis in hyperglycemic individuals could promote the formation of new vascular structures in the implanted patch such as endothelial and vascular smooth muscle cells. In summary, the SF/PU patch was no serious complications when implanted under hyperglycemia, and the patch was suitable to implant in diabetes ...
Feline morbillivirus (FeMV), a member of the family Paramyxoviridae, is an emerging virus that wa... more Feline morbillivirus (FeMV), a member of the family Paramyxoviridae, is an emerging virus that was discovered in 2012. Despite the importance of FeMV infection in cats because of its postulated involvement in kidney diseases, no simple serological assay has been reported in its detection. Here, FeMV phosphoprotein (P protein) was expressed and purified as a glutathione-S-transferase (GST)-fusion protein and used for an enzyme-linked immunosorbent assay (ELISA) to detect FeMV-specific antibodies. With a cutoff value determined by immunoblotting, anti-FeMV P protein was detected with this assay in 22 (22%) of the 100 cat plasma samples collected from various regions of Japan. This ELISA is useful for epidemiological and immunological studies, as well as for diagnosis of FeMV infection.
The vasovagal tonus index (VVTI) is a time-domain analysis method of heart rate variability acqui... more The vasovagal tonus index (VVTI) is a time-domain analysis method of heart rate variability acquired over a short period. It is a useful measurement for evaluating severity and prognosis heart failure in dogs. Behavior responses can be used to evaluate stress in each dog individually. Stress during clinical examination may interfere with the VVTI since it influences the sympathetic nervous system. The aim of this study was to investigate the effect of behavior responses during clinical examination on the VVTI. Data set obtained from physical examination, systolic blood pressure measurement, electrocardiography, VVTI calculation, and video recording were collected from 50 healthy dogs. Behavior scores were analyzed from video recording and dogs were classified into three groups; group 1 (passive), group 2 (quite active), and group 3 (highly active). The results showed that the VVTI was not different between the three groups (p=0.77). Medians and interquartiles of the VVTI in group 1,...
Feline morbillivirus (FmoPV) is a new virus species that was first discovered in Hong Kong in ₂₀₁... more Feline morbillivirus (FmoPV) is a new virus species that was first discovered in Hong Kong in ₂₀₁₂. Study of FmoPV is important because relationship between its infection and tubulointerstitial nephritis in cats was implicated in the first report. We attempted to develop a genetic detection system based on the consensus sequence of ₉ FmoPV genomic sequences in the L gene, since there were FmoPV strains undetectable by the previous RT-PCR system. When ₈₃ samples were tested with this system, we detected ₄ new FmoPV strains previously undetected by the previous RT-PCR. In order to develop an antibody detection system specific to a FmoPV-antigen, we expressed a recombinant FmoPV P protein in Escherichia coli as a fusion protein with glutathione-S-transferase and developed an ELISA with the purified P protein. We detected FmoPV in ₈₂% (₂₆/₃₄) of formalin-fixed kidney samples from cats suffered from nephritis, suggesting correlation between FmoPV infection and chronic nephritis. We are currently attempting to develop model systems to study FmoPVʼs pathogenicity using tissue culture cells or experimental animals. (J Anim Clin Med. 26(4)163-166, 2017) 要約:猫モルビリウイルス(FmoPV)は ₂₀₁₂ 年に香港で初めて発見された新しいウイルスであるが,感染と猫 の腎炎との関係が示唆されており今後の研究が重要である。私たちは FmoPV の病原性の解明のため,先ず疫学 に重要な遺伝子検査法を ₉ つの FmoPV 株の共通配列によって作製し,以前の方法で検出を行った ₈₃ サンプル中 に新たな ₄ 株の FmoPV を検出した。FmoPV 特異的抗原に対する抗体を検出するため,組み換え FmoPV P 蛋 白質を大腸菌で発現し,精製した蛋白質を用いて ELISA の作製を試みた。腎炎猫の固定腎臓組織サンプルから FmoPV の検出を行い,₈₂%(₂₆/₃₄)という高いパーセンテージで FmoPV が検出されたため,FmoPV の腎臓に おける病原性が確認された。現在,FmoPV の病原性のさらなる解明のため,培養細胞や実験動物を用いた病原性 モデルシステムの構築を進めている (動物臨床医学 ₂₆ (₄) ₁₆₃-₁₆₆, ₂₀₁₇)
Bats are reservoir hosts of many zoonotic viruses and identification of viruses that they carry i... more Bats are reservoir hosts of many zoonotic viruses and identification of viruses that they carry is important. This study aimed to use high throughput screening to identify the viruses in fecal guano of Taiwanese insectivorous bats caves in order to obtain more information on bat-derived pathogenic viruses in East Asia. Guano samples were collected from two caves in Taiwan, pooled, and then subjected to Multiplex PCR-based next generation sequencing for viral identification. Subsequently, encephalomyocarditis virus (EMCV) sequence was detected and confirmed by reverse transcription PCR. EMCV is considered as rodent virus and thus, animal species identification through cytochrome oxidase I (COI) barcoding was further done to identify the viral source. Finally, determination of distribution and verification of the presence of EMCV in guano obtained from Japanese and South Korean caves was also done. We concluded that the guano collected was not contaminated with the excrement of rodents which were reported and presumed to live in Taiwan. Also, EMCV genome fragments were found in guanos of Japanese and South Korean caves. It is possible that the eastern bent-wing bat (Miniopterus fuliginosus) is one of the natural hosts of EMCV in East Asia.
Feline morbillivirus (FeMV) is an emerging member of morbillivirus discovered in 2012. Although a... more Feline morbillivirus (FeMV) is an emerging member of morbillivirus discovered in 2012. Although association of FeMV infection with kidney diseases in cats has been suggested, the pathogenicity of the virus has not been clear to date. To study the association between FeMV infection and pathological changes in kidney tissues of infected cats, we performed immunohistochemistry and immunofluorescent assays to detect FeMV antigens and analyzed the effect of FeMV infection on the pathological changes in the kidney tissues. In 38 kidney tissue samples from cats, some tissue injury scores were significantly higher when the FeMV antigens were detected, especially those for the tubular tissues in which the FeMV antigens were mostly localized. Pathological changes associated with the FeMV antigens included the ones typically found in chronic kidney diseases, such as interstitial cell infiltration, glomerulosclerosis, tubular atrophy and fibrosis. We also detected some feline IgG localizations in glomerular tissues, though co-localization or significant association with the FeMV antigens were not found. Our study confirms the association of FeMV infection with some kidney tissue injuries and provides additional information about roles of FeMV infection in chronic kidney diseases.
Eosinophils like many myeloid innate immune cells can provide cytokines and chemokines for the ac... more Eosinophils like many myeloid innate immune cells can provide cytokines and chemokines for the activation of other immune cells upon TLR stimulation. When TLR stimulated eosinophils were inoculated i.p. into wildtype mice, NK cells were rapidly recruited and exhibited anti-tumor cytotoxicity. However when mice depleted of CD11c(+) cells were used, a marked decrease in the number of recruited NK cells was observed. We postulated that CpG or LPS from the injected eosinophils could be transferred to host cells, which in turn could recruit NK cells. However, by inoculating mice deficient in TLR4 or TLR9 with LPS or CpG stimulated eosinophils respectively, NK cell recruitment was still observed alongside cytotoxicity and IFNγ production. CpG-stimulation of eosinophils produced the pro-inflammatory cytokine IL-12 and the chemokine CXCL10, which are important for NK cell activation and recruitment in vivo. To demonstrate the importance of CXCL10 in NK cell recruitment, we found that CpG-st...
Feline morbillivirus (FeMV), a member of the family Paramyxoviridae, is an emerging virus that wa... more Feline morbillivirus (FeMV), a member of the family Paramyxoviridae, is an emerging virus that was discovered in 2012. Despite the importance of FeMV infection in cats because of its postulated involvement in kidney diseases, no simple serological assay has been reported in its detection. Here, FeMV phosphoprotein (P protein) was expressed and purified as a glutathione-S-transferase (GST)-fusion protein and used for an enzyme-linked immunosorbent assay (ELISA) to detect FeMV-specific antibodies. With a cutoff value determined by immunoblotting, anti-FeMV P protein was detected with this assay in 22 (22%) of the 100 cat plasma samples collected from various regions of Japan. This ELISA is useful for epidemiological and immunological studies, as well as for diagnosis of FeMV infection.
Eosinophils like many myeloid innate immune cells can provide cytokines and chemokines for the ac... more Eosinophils like many myeloid innate immune cells can provide cytokines and chemokines for the activation of other immune cells upon TLR stimulation. When TLR stimulated eosinophils were inoculated i.p. into wildtype mice, NK cells were rapidly recruited and exhibited anti-tumor cytotoxicity. However when mice depleted of CD11c+ cells were used, a marked decrease in the number of recruited NK cells was observed. We postulated that CpG or LPS from the injected eosinophils could be transferred to host cells, which in turn could recruit NK cells. However, by inoculating mice deficient in TLR4 or TLR9 with LPS or CpG stimulated eosinophils respectively, NK cell recruitment was still observed alongside cytotoxicity and IFNγ production. CpG-stimulation of eosinophils produced the pro-inflammatory cytokine IL-12 and the chemokine CXCL10, which are important for NK cell activation and recruitment in vivo. To demonstrate the importance of CXCL10 in NK cell recruitment, we found that CpG-stimulated eosinophils pre-treated with the gut microbial metabolite butyrate had reduced expression and production of CXCL10 and IL-12 and concomitantly were poor at recruitment of NK cells and inducing IFNγ in NK cells. Therefore, eosinophils like other innate immune cells of myeloid origin can conceivably stimulate NK cell activity. In addition, products of the gut microbiota can be potential inhibitors of NK cell activation and recruitment by dampening pro-inflammatory cytokines.
Lumpy skin disease (LSD) is an economically devastating and transboundary disease in cattle. Here... more Lumpy skin disease (LSD) is an economically devastating and transboundary disease in cattle. Here, we report the coding-complete genome sequence of the LSDV72/PrachuapKhiriKhan/Thailand/2021 strain, which was isolated from an affected cow during the first LSD outbreak in Thailand in 2021. The sequence will be beneficial for future genomic studies of the virus.
Purpose To understand the complication and histopathological characteristics between the Silk Fib... more Purpose To understand the complication and histopathological characteristics between the Silk Fibroin/Polyurethanes (SF/PU) and the host response, and to unveil the compatibility of the patch in diabetes individuals. Methods Rats were divided into DM and control (CT) groups, and the DM group was induced with streptozotocin. All groups underwent the SF/PU patch implantation in the abdominal aorta, and the implanted patches were evaluated at one, two, three, and four weeks after implantation. Results DM group had more fibrosis formation and a delayed endothelialization compared to the CT group. There was no evidence of chronic inflammation in both DM and CT groups. Conclusions Fibrosis in hyperglycemic individuals could promote the formation of new vascular structures in the implanted patch such as endothelial and vascular smooth muscle cells. In summary, the SF/PU patch was no serious complications when implanted under hyperglycemia, and the patch was suitable to implant in diabetes ...
Feline morbillivirus (FeMV), a member of the family Paramyxoviridae, is an emerging virus that wa... more Feline morbillivirus (FeMV), a member of the family Paramyxoviridae, is an emerging virus that was discovered in 2012. Despite the importance of FeMV infection in cats because of its postulated involvement in kidney diseases, no simple serological assay has been reported in its detection. Here, FeMV phosphoprotein (P protein) was expressed and purified as a glutathione-S-transferase (GST)-fusion protein and used for an enzyme-linked immunosorbent assay (ELISA) to detect FeMV-specific antibodies. With a cutoff value determined by immunoblotting, anti-FeMV P protein was detected with this assay in 22 (22%) of the 100 cat plasma samples collected from various regions of Japan. This ELISA is useful for epidemiological and immunological studies, as well as for diagnosis of FeMV infection.
The vasovagal tonus index (VVTI) is a time-domain analysis method of heart rate variability acqui... more The vasovagal tonus index (VVTI) is a time-domain analysis method of heart rate variability acquired over a short period. It is a useful measurement for evaluating severity and prognosis heart failure in dogs. Behavior responses can be used to evaluate stress in each dog individually. Stress during clinical examination may interfere with the VVTI since it influences the sympathetic nervous system. The aim of this study was to investigate the effect of behavior responses during clinical examination on the VVTI. Data set obtained from physical examination, systolic blood pressure measurement, electrocardiography, VVTI calculation, and video recording were collected from 50 healthy dogs. Behavior scores were analyzed from video recording and dogs were classified into three groups; group 1 (passive), group 2 (quite active), and group 3 (highly active). The results showed that the VVTI was not different between the three groups (p=0.77). Medians and interquartiles of the VVTI in group 1,...
Feline morbillivirus (FmoPV) is a new virus species that was first discovered in Hong Kong in ₂₀₁... more Feline morbillivirus (FmoPV) is a new virus species that was first discovered in Hong Kong in ₂₀₁₂. Study of FmoPV is important because relationship between its infection and tubulointerstitial nephritis in cats was implicated in the first report. We attempted to develop a genetic detection system based on the consensus sequence of ₉ FmoPV genomic sequences in the L gene, since there were FmoPV strains undetectable by the previous RT-PCR system. When ₈₃ samples were tested with this system, we detected ₄ new FmoPV strains previously undetected by the previous RT-PCR. In order to develop an antibody detection system specific to a FmoPV-antigen, we expressed a recombinant FmoPV P protein in Escherichia coli as a fusion protein with glutathione-S-transferase and developed an ELISA with the purified P protein. We detected FmoPV in ₈₂% (₂₆/₃₄) of formalin-fixed kidney samples from cats suffered from nephritis, suggesting correlation between FmoPV infection and chronic nephritis. We are currently attempting to develop model systems to study FmoPVʼs pathogenicity using tissue culture cells or experimental animals. (J Anim Clin Med. 26(4)163-166, 2017) 要約:猫モルビリウイルス(FmoPV)は ₂₀₁₂ 年に香港で初めて発見された新しいウイルスであるが,感染と猫 の腎炎との関係が示唆されており今後の研究が重要である。私たちは FmoPV の病原性の解明のため,先ず疫学 に重要な遺伝子検査法を ₉ つの FmoPV 株の共通配列によって作製し,以前の方法で検出を行った ₈₃ サンプル中 に新たな ₄ 株の FmoPV を検出した。FmoPV 特異的抗原に対する抗体を検出するため,組み換え FmoPV P 蛋 白質を大腸菌で発現し,精製した蛋白質を用いて ELISA の作製を試みた。腎炎猫の固定腎臓組織サンプルから FmoPV の検出を行い,₈₂%(₂₆/₃₄)という高いパーセンテージで FmoPV が検出されたため,FmoPV の腎臓に おける病原性が確認された。現在,FmoPV の病原性のさらなる解明のため,培養細胞や実験動物を用いた病原性 モデルシステムの構築を進めている (動物臨床医学 ₂₆ (₄) ₁₆₃-₁₆₆, ₂₀₁₇)
Bats are reservoir hosts of many zoonotic viruses and identification of viruses that they carry i... more Bats are reservoir hosts of many zoonotic viruses and identification of viruses that they carry is important. This study aimed to use high throughput screening to identify the viruses in fecal guano of Taiwanese insectivorous bats caves in order to obtain more information on bat-derived pathogenic viruses in East Asia. Guano samples were collected from two caves in Taiwan, pooled, and then subjected to Multiplex PCR-based next generation sequencing for viral identification. Subsequently, encephalomyocarditis virus (EMCV) sequence was detected and confirmed by reverse transcription PCR. EMCV is considered as rodent virus and thus, animal species identification through cytochrome oxidase I (COI) barcoding was further done to identify the viral source. Finally, determination of distribution and verification of the presence of EMCV in guano obtained from Japanese and South Korean caves was also done. We concluded that the guano collected was not contaminated with the excrement of rodents which were reported and presumed to live in Taiwan. Also, EMCV genome fragments were found in guanos of Japanese and South Korean caves. It is possible that the eastern bent-wing bat (Miniopterus fuliginosus) is one of the natural hosts of EMCV in East Asia.
Feline morbillivirus (FeMV) is an emerging member of morbillivirus discovered in 2012. Although a... more Feline morbillivirus (FeMV) is an emerging member of morbillivirus discovered in 2012. Although association of FeMV infection with kidney diseases in cats has been suggested, the pathogenicity of the virus has not been clear to date. To study the association between FeMV infection and pathological changes in kidney tissues of infected cats, we performed immunohistochemistry and immunofluorescent assays to detect FeMV antigens and analyzed the effect of FeMV infection on the pathological changes in the kidney tissues. In 38 kidney tissue samples from cats, some tissue injury scores were significantly higher when the FeMV antigens were detected, especially those for the tubular tissues in which the FeMV antigens were mostly localized. Pathological changes associated with the FeMV antigens included the ones typically found in chronic kidney diseases, such as interstitial cell infiltration, glomerulosclerosis, tubular atrophy and fibrosis. We also detected some feline IgG localizations in glomerular tissues, though co-localization or significant association with the FeMV antigens were not found. Our study confirms the association of FeMV infection with some kidney tissue injuries and provides additional information about roles of FeMV infection in chronic kidney diseases.
Eosinophils like many myeloid innate immune cells can provide cytokines and chemokines for the ac... more Eosinophils like many myeloid innate immune cells can provide cytokines and chemokines for the activation of other immune cells upon TLR stimulation. When TLR stimulated eosinophils were inoculated i.p. into wildtype mice, NK cells were rapidly recruited and exhibited anti-tumor cytotoxicity. However when mice depleted of CD11c(+) cells were used, a marked decrease in the number of recruited NK cells was observed. We postulated that CpG or LPS from the injected eosinophils could be transferred to host cells, which in turn could recruit NK cells. However, by inoculating mice deficient in TLR4 or TLR9 with LPS or CpG stimulated eosinophils respectively, NK cell recruitment was still observed alongside cytotoxicity and IFNγ production. CpG-stimulation of eosinophils produced the pro-inflammatory cytokine IL-12 and the chemokine CXCL10, which are important for NK cell activation and recruitment in vivo. To demonstrate the importance of CXCL10 in NK cell recruitment, we found that CpG-st...
Feline morbillivirus (FeMV), a member of the family Paramyxoviridae, is an emerging virus that wa... more Feline morbillivirus (FeMV), a member of the family Paramyxoviridae, is an emerging virus that was discovered in 2012. Despite the importance of FeMV infection in cats because of its postulated involvement in kidney diseases, no simple serological assay has been reported in its detection. Here, FeMV phosphoprotein (P protein) was expressed and purified as a glutathione-S-transferase (GST)-fusion protein and used for an enzyme-linked immunosorbent assay (ELISA) to detect FeMV-specific antibodies. With a cutoff value determined by immunoblotting, anti-FeMV P protein was detected with this assay in 22 (22%) of the 100 cat plasma samples collected from various regions of Japan. This ELISA is useful for epidemiological and immunological studies, as well as for diagnosis of FeMV infection.
Eosinophils like many myeloid innate immune cells can provide cytokines and chemokines for the ac... more Eosinophils like many myeloid innate immune cells can provide cytokines and chemokines for the activation of other immune cells upon TLR stimulation. When TLR stimulated eosinophils were inoculated i.p. into wildtype mice, NK cells were rapidly recruited and exhibited anti-tumor cytotoxicity. However when mice depleted of CD11c+ cells were used, a marked decrease in the number of recruited NK cells was observed. We postulated that CpG or LPS from the injected eosinophils could be transferred to host cells, which in turn could recruit NK cells. However, by inoculating mice deficient in TLR4 or TLR9 with LPS or CpG stimulated eosinophils respectively, NK cell recruitment was still observed alongside cytotoxicity and IFNγ production. CpG-stimulation of eosinophils produced the pro-inflammatory cytokine IL-12 and the chemokine CXCL10, which are important for NK cell activation and recruitment in vivo. To demonstrate the importance of CXCL10 in NK cell recruitment, we found that CpG-stimulated eosinophils pre-treated with the gut microbial metabolite butyrate had reduced expression and production of CXCL10 and IL-12 and concomitantly were poor at recruitment of NK cells and inducing IFNγ in NK cells. Therefore, eosinophils like other innate immune cells of myeloid origin can conceivably stimulate NK cell activity. In addition, products of the gut microbiota can be potential inhibitors of NK cell activation and recruitment by dampening pro-inflammatory cytokines.
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Papers by Kripitch Sutummaporn