Combinatorial plasmid libraries for material monomer production in Escherichia coli. - Design of ... more Combinatorial plasmid libraries for material monomer production in Escherichia coli. - Design of Experiments: To investigate the production of material monomer targets in E. coli, combinatorial plasmid libraries (carrying genes for enzyme steps in target biosynthetic pathways) were designed using a D-optimal design of experiments (DoE) approach. Varied factors include plasmid copy number (ColE1, p15a, BBR1 or SC101 origins of replication), promoter strength (Ptrc or PlacUV5 promoters), candidate gene selection, and sequential ordering of genes/promoters within the plasmid. Library sizes were selected in the range of 2-48 plasmids, depending on the number of factors and combinatorial space. The pdf file contains schematic illustrations of the plasmid libraries (Figures D1-18) along with DNA sequences of the PCR primers (Table D1) and bridging oligosaccharides (Table D2) required for LCR assembly of the libraries. - Learn: Analyses were performed on in vivo screening data for plasmid libraries designed to produce material monomer targets in E. coli. For each plasmid library several analyses were performed in order to assess their predictive capabilities, identify key factors and predict improved constructs. The pdf file collates reports containing the following information: Model fitting; Contrast and regression effects; Model diagnostics; DoE specifications; Predicted best combinations; Predicted best constructs; Design evaluation; Factor power analysis; and Samples relative variance.
Nutritional supplementation with fish oil or ω-3 (n-3) polyunsaturated fatty acids (PUFAs) has po... more Nutritional supplementation with fish oil or ω-3 (n-3) polyunsaturated fatty acids (PUFAs) has potential benefits for skin inflammation. Although the differential metabolism of the main n-3PUFA eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) could lead to distinct activities, there are no clinical studies comparing their relative efficacy in human skin. Following a 10-wk oral supplementation of healthy volunteers and using mass spectrometry-based lipidomics, we found that n-3PUFA mainly affected the epidermal mediator lipidome. EPA was more efficient than DHA in reducing production of arachidonic acid–derived lipids, and both n-3PUFA lowered N-acyl ethanolamines. In UV radiation–challenged skin (3 times the minimum erythemal dose), EPA attenuated the production of proinflammatory lipids, whereas DHA abrogated the migration of Langerhans cells, as assessed by immunohistochemistry. Interestingly, n-3PUFA increased the infiltration of CD4+ and CD8+ T cells but did not alter the erythemal response, either the sunburn threshold or the resolution of erythema, as assessed by spectrophotometric hemoglobin index readings. As EPA and DHA differentially impact cutaneous inflammation through changes in the network of epidermal lipids and dendritic and infiltrating immune cells, they should be considered separately when designing interventions for cutaneous disease.—Kendall, A. C., Pilkington, S. M., Murphy, S. A., Del Carratore, F., Sunarwidhi, A. L., Kiezel-Tsugunova, M., Urquhart, P., Watson, R. E. B., Breitling, R., Rhodes, L. E., Nicolaou, A. Dynamics of the human skin mediator lipidome in response to dietary ω-3 fatty acid supplementation.
This dataset is the precomputed actinobacterial clusters taken from the antiSMASH database, ready... more This dataset is the precomputed actinobacterial clusters taken from the antiSMASH database, ready to be loaded into OOPS.
Combinatorial plasmid libraries for material monomer production in Escherichia coli. - Design of ... more Combinatorial plasmid libraries for material monomer production in Escherichia coli. - Design of Experiments: To investigate the production of material monomer targets in E. coli, combinatorial plasmid libraries (carrying genes for enzyme steps in target biosynthetic pathways) were designed using a D-optimal design of experiments (DoE) approach. Varied factors include plasmid copy number (ColE1, p15a, BBR1 or SC101 origins of replication), promoter strength (Ptrc or PlacUV5 promoters), candidate gene selection, and sequential ordering of genes/promoters within the plasmid. Library sizes were selected in the range of 2-48 plasmids, depending on the number of factors and combinatorial space. The pdf file contains schematic illustrations of the plasmid libraries (Figures D1-18) along with DNA sequences of the PCR primers (Table D1) and bridging oligosaccharides (Table D2) required for LCR assembly of the libraries. - Learn: Analyses were performed on in vivo screening data for plasmid libraries designed to produce material monomer targets in E. coli. For each plasmid library several analyses were performed in order to assess their predictive capabilities, identify key factors and predict improved constructs. The pdf file collates reports containing the following information: Model fitting; Contrast and regression effects; Model diagnostics; DoE specifications; Predicted best combinations; Predicted best constructs; Design evaluation; Factor power analysis; and Samples relative variance.
Nutritional supplementation with fish oil or ω-3 (n-3) polyunsaturated fatty acids (PUFAs) has po... more Nutritional supplementation with fish oil or ω-3 (n-3) polyunsaturated fatty acids (PUFAs) has potential benefits for skin inflammation. Although the differential metabolism of the main n-3PUFA eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) could lead to distinct activities, there are no clinical studies comparing their relative efficacy in human skin. Following a 10-wk oral supplementation of healthy volunteers and using mass spectrometry-based lipidomics, we found that n-3PUFA mainly affected the epidermal mediator lipidome. EPA was more efficient than DHA in reducing production of arachidonic acid–derived lipids, and both n-3PUFA lowered N-acyl ethanolamines. In UV radiation–challenged skin (3 times the minimum erythemal dose), EPA attenuated the production of proinflammatory lipids, whereas DHA abrogated the migration of Langerhans cells, as assessed by immunohistochemistry. Interestingly, n-3PUFA increased the infiltration of CD4+ and CD8+ T cells but did not alter the erythemal response, either the sunburn threshold or the resolution of erythema, as assessed by spectrophotometric hemoglobin index readings. As EPA and DHA differentially impact cutaneous inflammation through changes in the network of epidermal lipids and dendritic and infiltrating immune cells, they should be considered separately when designing interventions for cutaneous disease.—Kendall, A. C., Pilkington, S. M., Murphy, S. A., Del Carratore, F., Sunarwidhi, A. L., Kiezel-Tsugunova, M., Urquhart, P., Watson, R. E. B., Breitling, R., Rhodes, L. E., Nicolaou, A. Dynamics of the human skin mediator lipidome in response to dietary ω-3 fatty acid supplementation.
This dataset is the precomputed actinobacterial clusters taken from the antiSMASH database, ready... more This dataset is the precomputed actinobacterial clusters taken from the antiSMASH database, ready to be loaded into OOPS.
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Papers by Rainer Breitling