LOCUS       Exported               12635 bp ds-DNA     circular SYN 29-JAN-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12635)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Jan 29, 2020 from SnapGene 5.0.6
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12635
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(84..106)
                     /label=3'RBO - BOB
                     /note="color: black; sequence: gtcgcgtaacttaggacttgtgc; 
                     added: 2020-01-29"
     primer_bind     complement(84..106)
                     /label=RBO-R
                     /note="color: black; sequence: GTCGCGTAACTTAGGACTTGTGC; 
                     added: 2020-01-29"
     CDS             1187..1816
                     /codon_start=1
                     /product="stability protein from Pseudomonas plasmid pVS1"
                     /label=pVS1 StaA
                     /note="color: #993366"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2250..3317
                     /codon_start=1
                     /product="replication protein from Pseudomonas plasmid 
                     pVS1"
                     /label=pVS1 RepA
                     /note="color: #993366"
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     misc_feature    3921..4061
                     /label=bom
                     /note="basis of mobility region from pBR322"
                     /note="color: #a6acb3"
     rep_origin      complement(4247..4835)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(5079..5870)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /note="color: #ccffcc"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     primer_bind     6060..6082
                     /label=5'LBO F - BOB
                     /note="color: black; sequence: cggtcaaggttctggaccagttg; 
                     added: 2020-01-29"
     primer_bind     6330..6349
                     /label=LBO - BOB
                     /note="color: black; sequence: gatgggctgcctgtatcgag; added:
                     2020-01-29"
     misc_feature    6398..6422
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
                     /note="color: #ffe4c4"
     primer_bind     6447..6466
                     /label=pZY101-LB-1 (604) F
                     /note="color: black; sequence: aacacattgcggacgttttt; added:
                     2020-01-29"
     primer_bind     6475..6494
                     /label=pTF52fromLB
                     /note="color: black; sequence: gaattaacgccgaattgctc; added:
                     2020-01-29"
     primer_bind     6538..6557
                     /label=NOS2-LB (465) R
                     /note="color: black; sequence: ATTAATGCAGCTGGCACGAC; added:
                     2020-01-29"
     protein_bind    6599..6620
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     promoter        6635..6665
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This forward directional feature has 3 segments:
                        1: 6635 .. 6640 / #ffffff / -35
                        2: 6641 .. 6658 / #ffffff
                        3: 6659 .. 6665 / #ffffff / -10"
     protein_bind    6673..6689
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     primer_bind     6697..6713
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     6933..6952
                     
                     /note="color: black; sequence: agacgacgtcgattttctgc; added:
                     2020-01-29"
     primer_bind     complement(7096..7115)
                     
                     /note="color: black; sequence: aatcttctgcccttgttcca; added:
                     2020-01-29"
     CDS             complement(7286..7837)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /note="color: #ccffcc"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     primer_bind     7321..7339
                     /label=FRT1 Scr R
                     /note="color: black; sequence: GTACCGGCAGGCTGAAGTC; added: 
                     2020-01-29"
     primer_bind     complement(7465..7484)
                     /label=BarEndF
                     /note="color: black; sequence: CTTCAAGAGCGTGGTCGCTG; added:
                     2020-01-29"
     primer_bind     7511..7531
                     /label=BarNosInsR
                     /note="color: black; sequence: gtgggtgtagagcgtggagcc; 
                     added: 2020-01-29"
     primer_bind     7511..7531
                     /label=BarNosInsRa
                     /note="color: black; sequence: GTGGGTGTAGAGCGTGGAGCC; 
                     added: 2020-01-29"
     primer_bind     7517..7535
                     /label=Bar35S (559) F
                     /note="color: black; sequence: gtagagcgtggagcccagt; added: 
                     2020-01-29"
     primer_bind     complement(7581..7599)
                     /label=pZY101-LB-2 (666) R
                     /note="color: black; sequence: gcaacgcctacgactggac; added: 
                     2020-01-29"
     primer_bind     complement(7619..7639)
                     /label=BarMidF
                     /note="color: black; sequence: GAGGTCGCCGGCATCGCCTAC; 
                     added: 2020-01-29"
     primer_bind     7760..7778
                     /label=FLPe Screen F
                     /note="color: black; sequence: gttgacgatggtgcagacc; added: 
                     2020-01-29"
     primer_bind     complement(7824..7840)
                     /label=BAMHI-FRT-BAR-NOS F
                     /note="color: black; sequence: 
                     TCAAGGATCCAGCCAGAAGTTCCTATTCtctagaaaGTATAGGAACTTCGTAACCATGA
                     GCCCAGAACG; added: 2020-01-29"
     promoter        complement(7990..8334)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     8034..8053
                     /label=35S-Ubi (698) F
                     /note="color: black; sequence: ttgcgaaggatagtgggatt; added:
                     2020-01-29"
     primer_bind     complement(8057..8076)
                     /label=Bar35S (559) R
                     /note="color: black; sequence: cactgacgtaagggatgacg; added:
                     2020-01-29"
     primer_bind     8336..8355
                     /label=puc8-Ubi-FLPe-NOS F
                     /note="color: black; sequence: ttgccctttggtcttctgag; added:
                     2020-01-29"
     primer_bind     8339..8362
                     /label=ptf101TeloF
                     /note="color: black; sequence: ccctttggtcttctgagactgtat; 
                     added: 2020-01-29"
     primer_bind     complement(8375..8394)
                     /label=IntegraseProbe1R
                     /note="color: black; sequence: cgacacgcttgtctactcca; added:
                     2020-01-29"
     primer_bind     8380..8399
                     /label=PZY101 MCS F
                     /note="color: black; sequence: tagacaagcgtgtcgtgctc; added:
                     2020-01-29"
     primer_bind     8380..8399
                     /label=V2 puc8-Ubi-FLPe-NOS F
                     /note="color: black; sequence: tagacaagcgtgtcgtgctc; added:
                     2020-01-29"
     promoter        8440..10431
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     8471..8491
                     /label=Ubi1F
                     /note="color: black; sequence: CTCTAGAGATAATGAGCATTG; 
                     added: 2020-01-29"
     primer_bind     8486..8503
                     /label=IntegraseProbe2F
                     /note="color: black; sequence: gcgcattgcatgtctaagtt; added:
                     2020-01-29"
     primer_bind     8528..8549
                     /label=Ubi1F1
                     /note="color: black; sequence: TTGTCACACTTGTTTGAAGTGC; 
                     added: 2020-01-29"
     primer_bind     8657..8680
                     /label=Ubi-1 (619) F
                     /note="color: black; sequence: GACATGGTCTAAAGGACAATTGAG; 
                     added: 2020-01-29"
     primer_bind     8686..8705
                     /label=Ubi2-F
                     /note="color: black; sequence: TGACAACAGGACTCTACAGT; added:
                     2020-01-29"
     primer_bind     complement(8716..8739)
                     /label=UbiPR1
                     /note="color: black; sequence: AAAAAGGAGAACACATGCACACTA; 
                     added: 2020-01-29"
     primer_bind     complement(8719..8739)
                     /label=35S-Ubi (698) R
                     /note="color: black; sequence: AAAAAGGAGAACACATGCACA; 
                     added: 2020-01-29"
     primer_bind     9041..9062
                     /label=Contig2Rvs2
                     /note="color: black; sequence: GAGTCTAACGGACACCAACCAG; 
                     added: 2020-01-29"
     primer_bind     9170..9189
                     /label=Contig2Rvs1
                     /note="color: black; sequence: CTGTCGGCATCCAGAAATTG; added:
                     2020-01-29"
     primer_bind     9227..9245
                     /label=Ubi-2 (435) F
                     /note="color: black; sequence: GGCCTCCTCCTCCTCTCAC; added: 
                     2020-01-29"
     primer_bind     complement(9256..9274)
                     /label=Ubi-1 (619) R
                     /note="color: black; sequence: GGAAAGGAATCCCCCGTAG; added: 
                     2020-01-29"
     primer_bind     complement(9377..9396)
                     /label=IntegraseProbe2R2
                     /note="color: black; sequence: gatttgggggagatctggtt; added:
                     2020-01-29"
     primer_bind     9490..9509
                     /label=Ubi1063F
                     /note="color: black; sequence: GGGCCCGGTAGTTCTACTTC; added:
                     2020-01-29"
     primer_bind     complement(9594..9616)
                     /label=Contig6Fwd1
                     /note="color: black; sequence: ACTGGCAAGTTAGCAATCAGAAC; 
                     added: 2020-01-29"
     primer_bind     complement(9612..9631)
                     /label=IntegraseProbe2R
                     /note="color: black; sequence: tccccaaagagaaacactgg; added:
                     2020-01-29"
     primer_bind     9629..9648
                     /label=Ubi-3 (430) F
                     /note="color: black; sequence: GGAATCCTGGGATGGCTCTA; added:
                     2020-01-29"
     primer_bind     complement(9659..9678)
                     /label=Ubi-2 (453) R
                     /note="color: black; sequence: TCATGAAATCGATCCCGTCT; added:
                     2020-01-29"
     primer_bind     9691..9710
                     /label=IntegraseProbe3F
                     /note="color: black; sequence: cgttgcatagggtttggttt; added:
                     2020-01-29"
     primer_bind     9753..9772
                     /label=Primer3Forward1
                     /note="color: black; sequence: CGGGTCATCTTTTCATGCTT; added:
                     2020-01-29"
     primer_bind     complement(9867..9886)
                     /label=Contig4Fwd3
                     /note="color: black; sequence: TGACACACACATACAGTTCCAAA; 
                     added: 2020-01-29"
     primer_bind     9992..10011
                     /label=Ubi-4 (448) F
                     /note="color: black; sequence: TTTTGTTCGCTTGGTTGTGA; added:
                     2020-01-29"
     primer_bind     complement(10000..10019)
                     /label=Ubi1592R
                     /note="color: black; sequence: CCACATCATCACAACCAAGC; added:
                     2020-01-29"
     primer_bind     complement(10034..10054)
                     /label=Ubi-3 (430) R
                     /note="color: black; sequence: CCGATCTAGAACGAATGAACG; 
                     added: 2020-01-29"
     primer_bind     complement(10095..10117)
                     /label=Contig4Fwd3
                     /note="color: black; sequence: TGACACACACATACAGTTCCAAA; 
                     added: 2020-01-29"
     primer_bind     10098..10123
                     /label=Ubi1660F
                     /note="color: black; sequence: GGAACTGTATGTGTGTGTCATACATC; 
                     added: 2020-01-29"
     primer_bind     10397..10420
                     /label=Ubi47-F
                     /note="color: black; sequence: GATGCTCACCCTGTTGTTTGGTGT; 
                     added: 2020-01-29"
     primer_bind     10397..10416
                     /label=Ubi-Flpe (574) F
                     /note="color: black; sequence: GATGCTCACCCTGTTGTTTG; added:
                     2020-01-29"
     primer_bind     complement(10418..10439)
                     /label=Ubi-4 (448) R
                     /note="color: black; sequence: GAGTCGACCTGCAGAAGTAACA; 
                     added: 2020-01-29"
     primer_bind     complement(10419..10440)
                     /label=Ubi3'R
                     /note="color: black; sequence: AGAGTCGACCTGCAGAAGTAAC; 
                     added: 2020-01-29"
     CDS             10465..11496
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /note="color: #993366"
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     primer_bind     10773..10783
                     /label=NOS2-LB (465) R
                     /note="color: black; sequence: ATTAATGCAGCTGGCACGAC; added:
                     2020-01-29"
     primer_bind     10849..10868
                     /label=Cre (447) F
                     /note="color: black; sequence: GAACGTGCAAAACAGGCTCT"
     primer_bind     10880..10899
                     /label=Cre (200) F
                     /note="color: black; sequence: GCACTGATTTCGACCAGGTT"
     primer_bind     complement(11060..11079)
                     /label=Cre (200) R
                     /note="color: black; sequence: GCTAACCAGCGTTTTCGTTC"
     primer_bind     complement(11276..11295)
                     /label=Cre (447) R
                     /note="color: black; sequence: ATCCTTAGCGCCGTAAATCA"
     primer_bind     complement(11607..11626)
                     /label=FLPe Seq R
                     /note="color: black; sequence: AACGTGGGTAGCACCAAAAC; added:
                     2020-01-29"
     primer_bind     11671..11686
                     /label=AscI-AttP-NOS F
                     /note="color: black; sequence: 
                     GAATGGCGCGCCAATGACCCCAACTGGGGTAACCTTTGAGTTCTCTCAGTTGGGGGAAC
                     CTGCAGATCGTTCAAACA; added: 2020-01-29"
     terminator      11674..11926
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
                     /note="color: #ffffff"
     primer_bind     11676..11695
                     /label=BarNosSeq2.1
                     /note="color: black; sequence: TCGTTCAAACATTTGGCAAT; added:
                     2020-01-29"
     primer_bind     complement(11712..11730)
                     /label=Contig4Fwd1
                     /note="color: black; sequence: AAGACCGGCAACAGGATTC; added: 
                     2020-01-29"
     primer_bind     complement(11816..11838)
                     /label=Contig4Fwd2
                     /note="color: black; sequence: TTGCGGGACTCTAATCATAAAAA; 
                     added: 2020-01-29"
     primer_bind     11822..11845
                     /label=BarNosSeq3.1
                     /note="color: black; sequence: TGATTAGAGTCCCGCAATTATACA; 
                     added: 2020-01-29"
     primer_bind     complement(11861..11881)
                     /label=NosTerm238R
                     /note="color: black; sequence: TTGCGCGCTATATTTTGTTTT; 
                     added: 2020-01-29"
     primer_bind     complement(11950..11969)
                     /label=Flpe-NOS1 (797) R
                     /note="color: black; sequence: CTCTCGATTGCTCATCGTCA; added:
                     2020-01-29"
     primer_bind     complement(12286..12302)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     complement(12303..12322)
                     /label=FLPe Screen R
                     /note="color: black; sequence: gttttcccagtcacgacgtt; added:
                     2020-01-29"
     primer_bind     complement(12306..12329)
                     /label=M13-23merF
                     /note="color: black; sequence: cgccagggttttcccagtcacgac; 
                     added: 2020-01-29"
     primer_bind     complement(12306..12322)
                     /label=M13-17merF
                     /note="color: black; sequence: gttttcccagtcacgac; added: 
                     2020-01-29"
     primer_bind     complement(12309..12327)
                     /label=V2 puc8-Ubi-FLPe-NOS R
                     /note="color: black; sequence: ccagggttttcccagtcac; added: 
                     2020-01-29"
     primer_bind     12336..12356
                     /label=5'RBO F - BOB
                     /note="color: black; sequence: aacttaatcgccttgcagcac; 
                     added: 2020-01-29"
     primer_bind     complement(12376..12394)
                     /label=V2 puc8-Ubi-FLPe-NOS R 2
                     /note="color: black; sequence: ggcctcttcgctattacgc; added: 
                     2020-01-29"
     primer_bind     complement(12430..12449)
                     /label=PZY101 Bkbone R
                     /note="color: black; sequence: ctcaagctcattcgccattc; added:
                     2020-01-29"
     primer_bind     complement(12453..12472)
                     /label=puc8-Ubi-FLPe-NOS R
                     /note="color: black; sequence: gggaaacgacaatctgatcc; added:
                     2020-01-29"
     primer_bind     complement(12467..12486)
                     /label=IntegraseProbe4R
                     /note="color: black; sequence: tttaaactgaaggcgggaaa; added:
                     2020-01-29"
     primer_bind     complement(12467..12486)
                     /label=puc8-Ubi-FLPe-NOS R 2
                     /note="color: black; sequence: tttaaactgaaggcgggaaa; added:
                     2020-01-29"
     misc_feature    12498..12522
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
                     /note="color: #ffe4c4"
     primer_bind     complement(12561..12580)
                     /label=PZY101 MCS R
                     /note="color: black; sequence: aaaccttttcacgccctttt; added:
                     2020-01-29"
     primer_bind     complement(12597..12617)
                     /label=RBO - BOB
                     /note="color: black; sequence: ctgtggttggcatgcacatac; 
                     added: 2020-01-29"
ORIGIN
        1 agtactttga tccaacccct ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag
       61 ccgtcttctg aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg
      121 cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac
      181 tagaaccgga gacattacgc catgaacaag agcgccgccg ctggcctgct gggctatgcc
      241 cgcgtcagca ccgacgacca ggacttgacc aaccaacggg ccgaactgca cgcggccggc
      301 tgcaccaagc tgttttccga gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc
      361 aggatgcttg accacctacg ccctggcgac gttgtgacag tgaccaggct agaccgcctg
      421 gcccgcagca cccgcgacct actggacatt gccgagcgca tccaggaggc cggcgcgggc
      481 ctgcgtagcc tggcagagcc gtgggccgac accaccacgc cggccggccg catggtgttg
      541 accgtgttcg ccggcattgc cgagttcgag cgttccctaa tcatcgaccg cacccggagc
      601 gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc cccgccctac cctcaccccg
      661 gcacagatcg cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg
      721 gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa
      781 gtgacgccca ccgaggccag gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc
      841 gacgccctgg cggccgccga gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg
      901 acggccagga cgaaccgttt ttcattaccg aagagatcga ggcggagatg atcgcggccg
      961 ggtacgtgtt cgagccgccc gcgcacgtct caaccgtgcg gctgcatgaa atcctggccg
     1021 gtttgtctga tgccaagctg gcggcctggc cggccagctt ggccgctgaa gaaaccgagc
     1081 gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg
     1141 cgtatatgat gcgatgagta aataaacaaa tacgcaaggg gaacgcatga aggttatcgc
     1201 tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc
     1261 cctgcaactc gccggggccg atgttctgtt agtcgattcc gatccccagg gcagtgcccg
     1321 cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg accgcccgac
     1381 gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc
     1441 ccaggcggcg gacttggctg tgtccgcgat caaggcagcc gacttcgtgc tgattccggt
     1501 gcagccaagc ccttacgaca tatgggccac cgccgacctg gtggagctgg ttaagcagcg
     1561 cattgaggtc acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg
     1621 cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg tacgagctgc ccattcttga
     1681 gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc gccgccggca caaccgttct
     1741 tgaatcagaa cccgagggcg acgctgcccg cgaggtccag gcgctggccg ctgaaattaa
     1801 atcaaaactc atttgagtta atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta
     1861 agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa cgttggccag cctggcagac
     1921 acgccagcca tgaagcgggt caactttcag ttgccggcgg aggatcacac caagctgaag
     1981 atgtacgcgg tacgccaagg caagaccatt accgagctgc tatctgaata catcgcgcag
     2041 ctaccagagt aaatgagcaa atgaataaat gagtagatga attttagcgg ctaaaggagg
     2101 cggcatggaa aatcaagaac aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg
     2161 aacgggcggt tggccaggcg taagcggctg ggttgtctgc cggccctgca atggcactgg
     2221 aacccccaag cccgaggaat cggcgtgacg gtcgcaaacc atccggcccg gtacaaatcg
     2281 gcgcggcgct gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc gcccagcggc
     2341 aacgcatcga ggcagaagca cgccccggtg aatcgtggca agcggccgct gatcgaatcc
     2401 gcaaagaatc ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg
     2461 gcgacgagca accagatttt ttcgttccga tgctctatga cgtgggcacc cgcgatagtc
     2521 gcagcatcat ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg
     2581 tgatccgcta cgagcttcca gacgggcacg tagaggtttc cgcagggccg gccggcatgg
     2641 ccagtgtgtg ggattacgac ctggtactga tggcggtttc ccatctaacc gaatccatga
     2701 accgataccg ggaagggaag ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg
     2761 acgtactcaa gttctgccgg cgagccgatg gcggaaagca gaaagacgac ctggtagaaa
     2821 cctgcattcg gttaaacacc acgcacgttg ccatgcagcg tacgaagaag gccaagaacg
     2881 gccgcctggt gacggtatcc gagggtgaag ccttgattag ccgctacaag atcgtaaaga
     2941 gcgaaaccgg gcggccggag tacatcgaga tcgagctagc tgattggatg taccgcgaga
     3001 tcacagaagg caagaacccg gacgtgctga cggttcaccc cgattacttt ttgatcgatc
     3061 ccggcatcgg ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca
     3121 gatggttgtt caagacgatc tacgaacgca gtggcagcgc cggagagttc aagaagttct
     3181 gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg
     3241 aggcggggca ggctggcccg atcctagtca tgcgctaccg caacctgatc gagggcgaag
     3301 catccgccgg ttcctaatgt acggagcaga tgctagggca aattgcccta gcaggggaaa
     3361 aaggtcgaaa aggtctcttt cctgtggata gcacgtacat tgggaaccca aagccgtaca
     3421 ttgggaaccg gaacccgtac attgggaacc caaagccgta cattgggaac cggtcacaca
     3481 tgtaagtgac tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac
     3541 ttattaaaac tcttaaaacc cgcctggcct gtgcataact gtctggccag cgcacagccg
     3601 aagagctgca aaaagcgcct acccttcggt cgctgcgctc cctacgcccc gccgcttcgc
     3661 gtcggcctat cgcggccgct ggccgctcaa aaatggctgg cctacggcca ggcaatctac
     3721 cagggcgcgg acaagccgcg ccgtcgccac tcgaccgccg gcgcccacat caaggcaccc
     3781 tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
     3841 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     3901 ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat
     3961 actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
     4021 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc
     4081 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     4141 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     4201 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     4261 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     4321 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     4381 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     4441 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     4501 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     4561 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     4621 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     4681 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     4741 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     4801 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     4861 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg catgatatat
     4921 ctcccaattt gtgtagggct tattatgcac gcttaaaaat aataaaagca gacttgacct
     4981 gatagtttgg ctgtgagcaa ttatgtgctt agtgcatcta atcgcttgag ttaacgccgg
     5041 cgaagcggcg tcggcttgaa cgaatttcta gctagacatt atttgccgac taccttggtg
     5101 atctcgcctt tcacgtagtg gacaaattct tccaactgat ctgcgcgcga ggccaagcga
     5161 tcttcttctt gtccaagata agcctgtcta gcttcaagta tgacgggctg atactgggcc
     5221 ggcaggcgct ccattgccca gtcggcagcg acatccttcg gcgcgatttt gccggttact
     5281 gcgctgtacc aaatgcggga caacgtaagc actacatttc gctcatcgcc agcccagtcg
     5341 ggcggcgagt tccatagcgt taaggtttca tttagcgcct caaatagatc ctgttcagga
     5401 accggatcaa agagttcctc cgccgctgga cctaccaagg caacgctatg ttctcttgct
     5461 tttgtcagca agatagccag atcaatgtcg atcgtggctg gctcgaagat acctgcaaga
     5521 atgtcattgc gctgccattc tccaaattgc agttcgcgct tagctggata acgccacgga
     5581 atgatgtcgt cgtgcacaac aatggtgact tctacagcgc ggagaatctc gctctctcca
     5641 ggggaagccg aagtttccaa aaggtcgttg atcaaagctc gccgcgttgt ttcatcaagc
     5701 cttacggtca ccgtaaccag caaatcaata tcactgtgtg gcttcaggcc gccatccact
     5761 gcggagccgt acaaatgtac ggccagcaac gtcggttcga gatggcgctc gatgacgcca
     5821 actacctctg atagttgagt cgatacttcg gcgatcaccg cttcccccat gatgtttaac
     5881 tttgttttag ggcgactgcc ctgctgcgta acatcgttgc tgctccataa catcaaacat
     5941 cgacccacgg cgtaacgcgc ttgctgcttg gatgcccgag gcatagactg taccccaaaa
     6001 aaacatgtca taacaagaag ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc
     6061 ggtcaaggtt ctggaccagt tgcgtgacgg cagttacgct acttgcatta cagcttacga
     6121 accgaacgag gcttatgtcc actgggttcg tgcccgaatt gatcacaggc agcaacgctc
     6181 tgtcatcgtt acaatcaaca tgctaccctc cgcgagatca tccgtgtttc aaacccggca
     6241 gcttagttgc cgttcttccg aatagcatcg gtaacatgag caaagtctgc cgccttacaa
     6301 cggctctccc gctgacgccg tcccggactg atgggctgcc tgtatcgagt ggtgattttg
     6361 tgccgagctg ccggtcgggg agctgttggc tggctggtgg caggatatat tgtggtgtaa
     6421 acaaattgac gcttagacaa cttaataaca cattgcggac gtttttaatg tactgaatta
     6481 acgccgaatt gctctagcca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     6541 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     6601 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     6661 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     6721 acaagctaat tccttcaaga cgtgctcaaa tcactatttc cacaccccta tatttctatt
     6781 gcactccctt ttaactgttt tttattacaa aaatgccctg gaaaatgcac tccctttttg
     6841 tgtttgtttt tttgtgaaac gatgttgtca ggtaatttat ttgtcagtct actatggtgg
     6901 cccattatat taatagcaac tgtcggtcca atagacgacg tcgattttct gcatttgttt
     6961 aaccacgtgg attttatgac attttatatt agttaatttg taaaacctac ccaattaaag
     7021 acctcatatg ttctaaagac taatacttaa tgataacaat tttcttttag tgaagaaagg
     7081 gataattagt aaatatggaa caagggcaga agatttatta aagccggtaa gagacaacaa
     7141 cgtaggtacg tggagtgtct taggtgactt acccacataa cataaagtga cattaacaaa
     7201 catagctaat gctcctattt gaatagtgca tatcagcata ccttattaca tatagatagg
     7261 agcaaactct agctagattg ttgagtcaga tctcggtgac gggcaggacc ggacggggcg
     7321 gtaccggcag gctgaagtcc agctgccaga aacccacgtc atgccagttc ccgtgcttga
     7381 agccggccgc ccgcagcatg ccgcgggggg catatccgag cgcctcgtgc atgcgcacgc
     7441 tcgggtcgtt gggcagcccg atgacagcga ccacgctctt gaagccctgt gcctccaggg
     7501 acttcagcag gtgggtgtag agcgtggagc ccagtcccgt ccgctggtgg cggggggaga
     7561 cgtacacggt cgactcggcc gtccagtcgt aggcgttgcg tgccttccag gggcccgcgt
     7621 aggcgatgcc ggcgacctcg ccgtccacct cggcgacgag ccagggatag cgctcccgca
     7681 gacggacgag gtcgtccgtc cactcctgcg gttcctgcgg ctcggtacgg aagttgaccg
     7741 tgcttgtctc gatgtagtgg ttgacgatgg tgcagaccgc cggcatgtcc gcctcggtgg
     7801 cacggcggat gtcggccggg cgtcgttctg ggctcatggt agatcccccc gttcgtaaat
     7861 ggtgaaaatt ttcagaaaat tgcttttgct ttaaaagaaa tgatttaaat tgctgcaata
     7921 aaagtagaat gcttgattgc ttgagattcg tttgttttgt atatgttgtg ttgagaatta
     7981 attctcgagg tcctctccaa atgaaatgaa cttccttata tagaggaagg gtcttgcgaa
     8041 ggatagtggg attgtgcgtc atcccttacg tcagtggaga tatcacatca atccacttgc
     8101 tttgaagacg tggttggaac gtcttctttt tccacgatgc tcctcgtggg tgggggtcca
     8161 tctttgggac cactgtcggc agaggcatct tcaacgatgg cctttccttt atcgcaatga
     8221 tggcatttgt aggagccacc ttccttttcc actatcttca caataaagtg acagatagct
     8281 gggcaatgga atccgaggag gtttccggat attacccttt gttgaaaagt ctcaattgcc
     8341 ctttggtctt ctgagactgt atctttgata tttttggagt agacaagcgt gtcgtgctcc
     8401 accatgttga cgaaggcatg caagcttaag cttgcatgcc tgcagtgcag cgtgacccgg
     8461 tcgtgcccct ctctagagat aatgagcatt gcatgtctaa gttataaaaa attaccacat
     8521 attttttttg tcacacttgt ttgaagtgca gtttatctat ctttatacat atatttaaac
     8581 tttactctac gaataatata atctatagta ctacaataat atcagtgttt tagagaatca
     8641 tataaatgaa cagttagaca tggtctaaag gacaattgag tattttgaca acaggactct
     8701 acagttttat ctttttagtg tgcatgtgtt ctcctttttt tttgcaaata gcttcaccta
     8761 tataatactt catccatttt attagtacat ccatttaggg tttagggtta atggttttta
     8821 tagactaatt tttttagtac atctatttta ttctatttta gcctctaaat taagaaaact
     8881 aaaactctat tttagttttt ttatttaata atttagatat aaaatagaat aaaataaagt
     8941 gactaaaaat taaacaaata ccctttaaga aattaaaaaa actaaggaaa catttttctt
     9001 gtttcgagta gataatgcca gcctgttaaa cgccgtcgac gagtctaacg gacaccaacc
     9061 agcgaaccag cagcgtcgcg tcgggccaag cgaagcagac ggcacggcat ctctgtcgct
     9121 gcctctggac ccctctcgag agttccgctc caccgttgga cttgctccgc tgtcggcatc
     9181 cagaaattgc gtggcggagc ggcagacgtg agccggcacg gcaggcggcc tcctcctcct
     9241 ctcacggcac ggcagctacg ggggattcct ttcccaccgc tccttcgctt tcccttcctc
     9301 gcccgccgta ataaatagac accccctcca caccctcttt ccccaacctc gtgttgttcg
     9361 gagcgcacac acacacaacc agatctcccc caaatccacc cgtcggcacc tccgcttcaa
     9421 ggtacgccgc tcgtcctccc cccccccccc tctctacctt ctctagatcg gcgttccggt
     9481 ccatggttag ggcccggtag ttctacttct gttcatgttt gtgttagatc cgtgtttgtg
     9541 ttagatccgt gctgctagcg ttcgtacacg gatgcgacct gtacgtcaga cacgttctga
     9601 ttgctaactt gccagtgttt ctctttgggg aatcctggga tggctctagc cgttccgcag
     9661 acgggatcga tttcatgatt ttttttgttt cgttgcatag ggtttggttt gcccttttcc
     9721 tttatttcaa tatatgccgt gcacttgttt gtcgggtcat cttttcatgc ttttttttgt
     9781 cttggttgtg atgatgtggt ctggttgggc ggtcgttcta gatcggagta gaattctgtt
     9841 tcaaactacc tggtggattt attaattttg gatctgtatg tgtgtgccat acatattcat
     9901 agttacgaat tgaagatgat ggatggaaat atcgatctag gataggtata catgttgatg
     9961 cgggttttac tgatgcatat acagagatgc tttttgttcg cttggttgtg atgatgtggt
    10021 gtggttgggc ggtcgttcat tcgttctaga tcggagtaga atactgtttc aaactacctg
    10081 gtgtatttat taattttgga actgtatgtg tgtgtcatac atcttcatag ttacgagttt
    10141 aagatggatg gaaatatcga tctaggatag gtatacatgt tgatgtgggt tttactgatg
    10201 catatacatg atggcatatg cagcatctat tcatatgctc taaccttgag tacctatcta
    10261 ttataataaa caagtatgtt ttataattat tttgatcttg atatacttgg atgatggcat
    10321 atgcagcagc tatatgtgga tttttttagc cctgccttca tacgctattt atttgcttgg
    10381 tactgtttct tttgtcgatg ctcaccctgt tgtttggtgt tacttctgca ggtcgactct
    10441 agaggatccc cgggtaccgt tgacatgtcc aatttactga ccgtacacca aaatttgcct
    10501 gcattaccgg tcgatgcaac gagtgatgag gttcgcaaga acctgatgga catgttcagg
    10561 gatcgccagg cgttttctga gcatacctgg aaaatgcttc tgtccgtttg ccggtcgtgg
    10621 gcggcatggt gcaagttgaa taaccggaaa tggtttcccg cagaacctga agatgttcgc
    10681 gattatcttc tatatcttca ggcgcgcggt ctggcagtaa aaactatcca gcaacatttg
    10741 ggccagctaa acatgcttca tcgtcggtcc gggctgccac gaccaagtga cagcaatgct
    10801 gtttcactgg ttatgcggcg gatccgaaaa gaaaacgttg atgccggtga acgtgcaaaa
    10861 caggctctag cgttcgaacg cactgatttc gaccaggttc gttcactcat ggaaaatagc
    10921 gatcgctgcc aggatatacg taatctggca tttctgggga ttgcttataa caccctgtta
    10981 cgtatagccg aaattgccag gatcagggtt aaagatatct cacgtactga cggtgggaga
    11041 atgttaatcc atattggcag aacgaaaacg ctggttagca ccgcaggtgt agagaaggca
    11101 cttagcctgg gggtaactaa actggtcgag cgatggattt ccgtctctgg tgtagctgat
    11161 gatccgaata actacctgtt ttgccgggtc agaaaaaatg gtgttgccgc gccatctgcc
    11221 accagccagc tatcaactcg cgccctggaa gggatttttg aagcaactca tcgattgatt
    11281 tacggcgcta aggatgactc tggtcagaga tacctggcct ggtctggaca cagtgcccgt
    11341 gtcggagccg cgcgagatat ggcccgcgct ggagtttcaa taccggagat catgcaagct
    11401 ggtggctgga ccaatgtaaa tattgtcatg aactatatcc gtaacctgga tagtgaaaca
    11461 ggggcaatgg tgcgcctgct ggaagatggc gattagtaag aattcgcatg cattcatcaa
    11521 tattattcat gcggggaaag gcaagattaa tccaactggc aaatcatcca gcgtgattgg
    11581 taacttcagt tccagcgact tgattcgttt tggtgctacc cacgttttca ataaggacga
    11641 gatggtggag taaagaagga gtgcgtcgaa gcagatcgtt caaacatttg gcaataaagt
    11701 ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt tctgttgaat
    11761 tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag atgggttttt
    11821 atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat atagcgcgca
    11881 aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcgatc aaacttcggc
    11941 actgtgtaat gacgatgagc aatcgagagg ctgactaaca aaaggtatgc ccaaaaacaa
    12001 cctctccaaa ctgtttcgaa ttggaagttt ctgctcatgc cgacaggcat aacttanata
    12061 ttcgcgggct attcccacta attcgtcctg ctggtttgcg ccaagataaa tcagtgcatc
    12121 tccttacaag ttcctctgtc ttgtgaaatg aactgctgac tgccccccaa gaaagcctcc
    12181 tcatctccca gttggcggcg gctgatacac catcgaaaac ccacgtccga acacttgata
    12241 catgtgcctg agaaataggc ctacgtccaa gagctcgaat tcggcactgg ccgtcgtttt
    12301 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
    12361 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
    12421 gcgcagcctg aatggcgaat gagcttgagc ttggatcaga ttgtcgtttc ccgccttcag
    12481 tttaaactat cagtgtttga caggatatat tggcgggtaa acctaagaga aaagagcgtt
    12541 tattagaata acggatattt aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat
    12601 gtgcatgcca accacagggt tcccctcggg atcaa
//
