Tetracapsuloides bryosalmonae is a malacosporean parasite causing Proliferative Kidney Disease (P... more Tetracapsuloides bryosalmonae is a malacosporean parasite causing Proliferative Kidney Disease (PKD) of salmonids, which develops in a sac‐shaped phase producing infective spores in freshwater bryozoans. From 2001–2003, several samples of bryozoans were collected along the Sile River (Treviso Province, northern Italy) from sites which are in close communication with two trout farms where PKD is enzootic. All the colonies found were observed under a stereomicroscope in search of T. bryosalmonae and to isolate statoblasts. The bryozoans were classified by studying the superficial microarchitecture of the statoblasts using a scanning electron microscope (SEM). The following species were identified: Cristatella mucedo, Fredericella sultana, Plumatella casmiana, P. emarginata, P. fungosa, P. repens, P. reticulata, P. rugosa, and P. similirepens. Developmental stages of T. bryosalmonae were never found in the colonies. In 11 out of 40 P. fungosa floatoblasts, treated with KOH for SEM study, vermiform structures of 0.5–8 mm in length were observed. The possibility that these structures could represent the vermiform phase of T. bryosalmonae with a developmental stage similar to those described for Buddenbrockia plumatellae is discussed.
The combined use of morphological and molecular studies allowed for the first time the recognitio... more The combined use of morphological and molecular studies allowed for the first time the recognition and description of the adult stage of Clinostomum cutaneum Paperna, 1964 from the grey heron Ardea cinerea L. in Kenya. A redescription of the metacercaria that infect Nile tilapia Oreochromis niloticus niloticus (L.) from the same aquatic environment is also presented. C. cutaneum differs from all other species of Clinostomum Leidy, 1856 in the shape of its uterus. Sequencing the rRNA confirmed the morphological similarity between adults from the grey heron and the metacercarial stage from tilapia, and a level of genetic similarity with the other previously sequenced Clinostomum spp. was observed. The need for a reorganisation of Clinostomum using both morphological and molecular methods is highlighted.
Tetracapsuloides bryosalmonae is a malacosporean parasite causing Proliferative Kidney Disease (P... more Tetracapsuloides bryosalmonae is a malacosporean parasite causing Proliferative Kidney Disease (PKD) of salmonids, which develops in a sac‐shaped phase producing infective spores in freshwater bryozoans. From 2001–2003, several samples of bryozoans were collected along the Sile River (Treviso Province, northern Italy) from sites which are in close communication with two trout farms where PKD is enzootic. All the colonies found were observed under a stereomicroscope in search of T. bryosalmonae and to isolate statoblasts. The bryozoans were classified by studying the superficial microarchitecture of the statoblasts using a scanning electron microscope (SEM). The following species were identified: Cristatella mucedo, Fredericella sultana, Plumatella casmiana, P. emarginata, P. fungosa, P. repens, P. reticulata, P. rugosa, and P. similirepens. Developmental stages of T. bryosalmonae were never found in the colonies. In 11 out of 40 P. fungosa floatoblasts, treated with KOH for SEM study, vermiform structures of 0.5–8 mm in length were observed. The possibility that these structures could represent the vermiform phase of T. bryosalmonae with a developmental stage similar to those described for Buddenbrockia plumatellae is discussed.
The combined use of morphological and molecular studies allowed for the first time the recognitio... more The combined use of morphological and molecular studies allowed for the first time the recognition and description of the adult stage of Clinostomum cutaneum Paperna, 1964 from the grey heron Ardea cinerea L. in Kenya. A redescription of the metacercaria that infect Nile tilapia Oreochromis niloticus niloticus (L.) from the same aquatic environment is also presented. C. cutaneum differs from all other species of Clinostomum Leidy, 1856 in the shape of its uterus. Sequencing the rRNA confirmed the morphological similarity between adults from the grey heron and the metacercarial stage from tilapia, and a level of genetic similarity with the other previously sequenced Clinostomum spp. was observed. The need for a reorganisation of Clinostomum using both morphological and molecular methods is highlighted.
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