In this work, mesoporous silica nanoparticles (MSNs) were synthesized and loaded by curcumin (MSN... more In this work, mesoporous silica nanoparticles (MSNs) were synthesized and loaded by curcumin (MSNs-CU) and capped by chitosan glycerol phosphate hydrogel (CS/βGH/MSNs-CU). Both of CS/βGH/MSNs-CU and MSNs-CU were characterized and analyzed, then, the cell viability of epithelial breast tumor cells (MCF-7) and healthy primary human mammary fibroblasts (HPHMF) treated by these nanocarriers, free-CU and the CU dispersed in CS/βGH hydrogel were studied. The CU release of CS/βGH, CS/βGH/MSNs-CU, and MSNs-CU was studied in the simulated body fluid (SBF) and results showed CU release from CS/GP/CU, MSNs-CU and CS/βGP/MSNs-CU was 4, 13.19, 8.87% after 24h, respectively. Optimum loading and encapsulation efficiency percentages of CU into MSNs were obtained 25% and 99% in 24 h, respectively. The MTT assay showed MSNs, MSNs-CU, CU, CS/βGP, CS/βGP/CU, CS/βGP/MSNs-CU had no influence on HPHMF cells. Also, free-CU, MSNs-CU showed cytotoxicity in the concentrations upper than 100 µg/mL and 10 µg/mL...
International Journal of NanoScience and Nanotechnology, 2010
Carbon nanotubes are regarded as promising building blocks for the construction of novel biosenso... more Carbon nanotubes are regarded as promising building blocks for the construction of novel biosensors due to their unique properties like fast electron transfer, high electrocatalytic effect and good biocompatibility. In the present study, the effect of different chemical oxidation’s methods of multi-walled carbon nanotubes (MWCNTs) on the electrochemical behavior of modified graphite electrodes has been investigated. The MWCNTs were oxidized by using three kinds of oxidants: a) concentrated nitric acid (65 wt %), b) mixture of concentrated sulfuric acid (98 wt %) and nitric acid (65 wt %) (3:1), and c) hydrochloric acid (2M) followed by acid mixture of (b). The results of oxidation procedures were characterized by infrared spectroscopy. Graphite electrode surface was modified with cationic, anionic, and non-ionic surfactants. The obtained results showed that amperometric responses of functionalized CNTs were enhanced with cationic surfactant-modified electrodes. The sensitivity of ni...
Low mechanical strength and untargeted osteoinduction of chitosan hydrogel limit its application ... more Low mechanical strength and untargeted osteoinduction of chitosan hydrogel limit its application for bone regeneration. This study aimed to develop an injectable chitosan hydrogel with enhanced mechanical strength and improved osteoinductivity for bone tissue engineering. For this purpose, chitosan-modified halloysite nanotubes (mHNTs) were synthesized first. Then, icariin as a bone inducer was loaded into mHNTs (IC@mHNTs), resulting in a sustained drug release system. Further, nanocomposite chitosan/mHNTs hydrogels were prepared by the sol-gel transition, leading to decreased gelation time and temperature and enhanced mechanical strength of the resulting scaffolds. The mesenchymal stem cells were encapsulated into the hydrogels, and in vitro viability assays showed scaffold biocompatibility. Moreover, embedded mHNTs or IC@mHNTs in the scaffold resulted in enhanced proliferation and bone differentiation of encapsulated cells. It was collectively demonstrated that the injectable in situ forming nanocomposite chitosan hydrogel loaded with IC@mHNTs is a promising candidate for bone regeneration.
The main objective of this novel study was to develop midazolam hydrochloride Fast dissolving ora... more The main objective of this novel study was to develop midazolam hydrochloride Fast dissolving oral films (FDFs) using solvent casting method and to evaluate the characteristics of the optimum formulation through in vitro and in vivo analysis. The FDFs are new favorable solid dosage forms that deliver drugs rapidly to the blood circulation system and have great advantages in the emergent control of severe neuropathic attacks in children.Methods and Midazolam nanosuspensions were prepared using the ultrasonic method and then incorporated in the hydroxypropyl methyl cellulose (HPMC)/pullulan polymeric matrix with other excipients like glycerol and cellulose nanofiber as a softener and a compatibilizer, respectively. The prepared films were evaluated for mechanical properties, morphology study, disintegration time, and dissolution time. SEM images of FDFs showed the uniform distribution of spherical nanoparticles in the polymeric matrix. A film with 36% HPMC, 64% pullulan, and 21% glyce...
Rivastigmine hydrogen tartrate (RT) is a molecule with both hydrophilic and hydrophobic propertie... more Rivastigmine hydrogen tartrate (RT) is a molecule with both hydrophilic and hydrophobic properties used for the treatment of the Alzheimer's disease. In this work, the larger pore size of mesoporous silica nanoparticles (P1-MSN) was synthesized and then, P1-MSN were functionalized by succinic anhydride (S-P1-MSN) and 3-aminopropyltriethoxysilane (APTES) (AP-CO-P1-MSN) using the grafting and co-condensation methods, respectively. A new method was used for the functionalization of P1-MSN by succinic anhydride at room temperature. Nanoparticles were characterized by special instrumental analysis and loaded by RT. Maximum entrapment efficiency and RT loading percentage into P1-MSN, AP-CO-P1-MSN and S-P1-MSN were respectively obtained as 21.26 and 25.5%, 41.5 and 49.8%, and 11.9 and 14.28% for 24 h. In the simulated gastric and body fluids, the release rate of RT-loaded AP-CO-P1-MSN (AP-CO-P1-MSN-RT) was lower than that of other RT-loaded nanoparticles. In oral pathway, the sustained...
This study investigated the cellular uptake of fluorescein isothiocyanate-labelled mesoporous sil... more This study investigated the cellular uptake of fluorescein isothiocyanate-labelled mesoporous silica nanoparticles (FITC-MSNs), amine-functionalised FITC-MSNs (AP-FITC-MSNs) and their gallic acid (GA)-loaded counterparts. Mesoporous silica nanoparticles were labelled with fluorescein isothiocyanate, functionalised by 3-aminopropyltriethoxysilane (APTES) (AP-FITC-MSNs) and then loaded by GA. All nanoparticles were characterised by transmission electron microscopy (TEM), Fourier transform infrared spectroscopy, and X-ray diffraction. The cytotoxicity of different concentrations of dyed nanoparticles was investigated using (3-(4,5-trihydroxybenzoic acid, dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and flow cytometry. TEM images showed that the average particle sizes of FITC-MSNs and AP-FITC-MSNs were about 100 and 110 nm, respectively. These nanoparticles were internalised by Caco-2 cells, accumulated and dispersed into the cytoplasm, nucleus, and subcellular organelles. Nanoparticles containing GA clearly decreased the viability of cells. FITC-MSNs showed no toxicity on Caco-2 cells at concentrations of ≤50 µg/ml. Functionalisation of FITC-MSNs using APTES decreased toxicity effects on the cells. It was found that FITC-MSNs can be applied at low concentrations as a marker in the cells. In addition, AP-FITC-MSNs showed better biocompatibility with Caco-2 cells than FITC-MSNs, because of their positive surface charges.
2012 19th Iranian Conference of Biomedical Engineering (ICBME), 2012
Intelligent thermosensitive formulations based on PLGA-PEG-PLGA triblock copolymer have great pot... more Intelligent thermosensitive formulations based on PLGA-PEG-PLGA triblock copolymer have great potential in biomedical applications, due to their good biocompatibility, biodegradability and injectability. The gelation behavior of the synthesized PLGA-PEG-PLGA triblocks was investigated in this study and the effect of PEG molecular weight and lactide to glycolide molar ratio, as well as the reaction time was investigated. Triblocks with PEG1000 have shown inappropriate gelation; while those with higher molecular weight formed gel at body temperature. The insulin release profiles showed an approximately linear release profile without a non-desirable burst effect More than 80% of the insulin was released during 4 weeks.
Abstract Gelation time and degradation rate of thermally-sensitive aqueous solutions of chitosan/... more Abstract Gelation time and degradation rate of thermally-sensitive aqueous solutions of chitosan/Gp (glycerophosphate disodium salt) have been studied. The effects of different parameters such as Gp salt concentration, solution temperature, degree of deacetylation ...
Biomedical Engineering Group, Chemical Engineering Faculty, Tarbiat Modares University, Tehran, I... more Biomedical Engineering Group, Chemical Engineering Faculty, Tarbiat Modares University, Tehran, Iran, P.O. Box: 14155-143 Abstract Mucoadhesive polymers were first used in the early 1980s to design mucoadhesive buccal drug delivery systems. These systems have been introduced as novel drug delivery systems due to the ability to stick and stay on mucus membranes and slow release of the drug, and are a suitable alternative to overcome the problems of common oral drug delivery systems. Mucoadhesive systems can be prepared in various forms, such as tablet, gel, oral film, spray, viscose solution and micro/ nanoparticle, and can be used for drug delivery from the buccal, sublingual, intestine, ocular, nasal, vaginal, and other mucosal routes. Mucoadhesive polymers wich are the most important component of these drug delivery systems, are usually natural, synthetic or seminatural hydrophilic macro-molecules, containing functional groups forming hydrogen bonds, such as carboxyl and hydroxyl ...
Key Words chitosan properties, chitosan microparticles, drug delivery, spray drying, ionic gelati... more Key Words chitosan properties, chitosan microparticles, drug delivery, spray drying, ionic gelation Polymerization Quarterly, 2016 T he development of a new drug molecule is an expensive and time consuming effort. Improving the safety efficacy ratio of old drugs has been attempted by different methods focused on each individual drug therapy, dose specification and therapeutic drug monitoring. Controlled rate delivery, slow delivery and targeted delivery are other very promising methods which have been pursued intensively. Methods for increasing bioavailability, diminishing side-effects, promoting targeted delivery and treating conditions such as blood-brain barrier disorders, are the main topics in novel drug delivery explorations. Choosing a suitable polymer is one of the most important aspects in development of a drug delivery system. In the last three decades, avast number of biomedical applications have been reported for a chitin derivative, known as chitosan. In this overview t...
In this work, mesoporous silica nanoparticles (MSNs) were synthesized and loaded by curcumin (MSN... more In this work, mesoporous silica nanoparticles (MSNs) were synthesized and loaded by curcumin (MSNs-CU) and capped by chitosan glycerol phosphate hydrogel (CS/βGH/MSNs-CU). Both of CS/βGH/MSNs-CU and MSNs-CU were characterized and analyzed, then, the cell viability of epithelial breast tumor cells (MCF-7) and healthy primary human mammary fibroblasts (HPHMF) treated by these nanocarriers, free-CU and the CU dispersed in CS/βGH hydrogel were studied. The CU release of CS/βGH, CS/βGH/MSNs-CU, and MSNs-CU was studied in the simulated body fluid (SBF) and results showed CU release from CS/GP/CU, MSNs-CU and CS/βGP/MSNs-CU was 4, 13.19, 8.87% after 24h, respectively. Optimum loading and encapsulation efficiency percentages of CU into MSNs were obtained 25% and 99% in 24 h, respectively. The MTT assay showed MSNs, MSNs-CU, CU, CS/βGP, CS/βGP/CU, CS/βGP/MSNs-CU had no influence on HPHMF cells. Also, free-CU, MSNs-CU showed cytotoxicity in the concentrations upper than 100 µg/mL and 10 µg/mL...
International Journal of NanoScience and Nanotechnology, 2010
Carbon nanotubes are regarded as promising building blocks for the construction of novel biosenso... more Carbon nanotubes are regarded as promising building blocks for the construction of novel biosensors due to their unique properties like fast electron transfer, high electrocatalytic effect and good biocompatibility. In the present study, the effect of different chemical oxidation’s methods of multi-walled carbon nanotubes (MWCNTs) on the electrochemical behavior of modified graphite electrodes has been investigated. The MWCNTs were oxidized by using three kinds of oxidants: a) concentrated nitric acid (65 wt %), b) mixture of concentrated sulfuric acid (98 wt %) and nitric acid (65 wt %) (3:1), and c) hydrochloric acid (2M) followed by acid mixture of (b). The results of oxidation procedures were characterized by infrared spectroscopy. Graphite electrode surface was modified with cationic, anionic, and non-ionic surfactants. The obtained results showed that amperometric responses of functionalized CNTs were enhanced with cationic surfactant-modified electrodes. The sensitivity of ni...
Low mechanical strength and untargeted osteoinduction of chitosan hydrogel limit its application ... more Low mechanical strength and untargeted osteoinduction of chitosan hydrogel limit its application for bone regeneration. This study aimed to develop an injectable chitosan hydrogel with enhanced mechanical strength and improved osteoinductivity for bone tissue engineering. For this purpose, chitosan-modified halloysite nanotubes (mHNTs) were synthesized first. Then, icariin as a bone inducer was loaded into mHNTs (IC@mHNTs), resulting in a sustained drug release system. Further, nanocomposite chitosan/mHNTs hydrogels were prepared by the sol-gel transition, leading to decreased gelation time and temperature and enhanced mechanical strength of the resulting scaffolds. The mesenchymal stem cells were encapsulated into the hydrogels, and in vitro viability assays showed scaffold biocompatibility. Moreover, embedded mHNTs or IC@mHNTs in the scaffold resulted in enhanced proliferation and bone differentiation of encapsulated cells. It was collectively demonstrated that the injectable in situ forming nanocomposite chitosan hydrogel loaded with IC@mHNTs is a promising candidate for bone regeneration.
The main objective of this novel study was to develop midazolam hydrochloride Fast dissolving ora... more The main objective of this novel study was to develop midazolam hydrochloride Fast dissolving oral films (FDFs) using solvent casting method and to evaluate the characteristics of the optimum formulation through in vitro and in vivo analysis. The FDFs are new favorable solid dosage forms that deliver drugs rapidly to the blood circulation system and have great advantages in the emergent control of severe neuropathic attacks in children.Methods and Midazolam nanosuspensions were prepared using the ultrasonic method and then incorporated in the hydroxypropyl methyl cellulose (HPMC)/pullulan polymeric matrix with other excipients like glycerol and cellulose nanofiber as a softener and a compatibilizer, respectively. The prepared films were evaluated for mechanical properties, morphology study, disintegration time, and dissolution time. SEM images of FDFs showed the uniform distribution of spherical nanoparticles in the polymeric matrix. A film with 36% HPMC, 64% pullulan, and 21% glyce...
Rivastigmine hydrogen tartrate (RT) is a molecule with both hydrophilic and hydrophobic propertie... more Rivastigmine hydrogen tartrate (RT) is a molecule with both hydrophilic and hydrophobic properties used for the treatment of the Alzheimer's disease. In this work, the larger pore size of mesoporous silica nanoparticles (P1-MSN) was synthesized and then, P1-MSN were functionalized by succinic anhydride (S-P1-MSN) and 3-aminopropyltriethoxysilane (APTES) (AP-CO-P1-MSN) using the grafting and co-condensation methods, respectively. A new method was used for the functionalization of P1-MSN by succinic anhydride at room temperature. Nanoparticles were characterized by special instrumental analysis and loaded by RT. Maximum entrapment efficiency and RT loading percentage into P1-MSN, AP-CO-P1-MSN and S-P1-MSN were respectively obtained as 21.26 and 25.5%, 41.5 and 49.8%, and 11.9 and 14.28% for 24 h. In the simulated gastric and body fluids, the release rate of RT-loaded AP-CO-P1-MSN (AP-CO-P1-MSN-RT) was lower than that of other RT-loaded nanoparticles. In oral pathway, the sustained...
This study investigated the cellular uptake of fluorescein isothiocyanate-labelled mesoporous sil... more This study investigated the cellular uptake of fluorescein isothiocyanate-labelled mesoporous silica nanoparticles (FITC-MSNs), amine-functionalised FITC-MSNs (AP-FITC-MSNs) and their gallic acid (GA)-loaded counterparts. Mesoporous silica nanoparticles were labelled with fluorescein isothiocyanate, functionalised by 3-aminopropyltriethoxysilane (APTES) (AP-FITC-MSNs) and then loaded by GA. All nanoparticles were characterised by transmission electron microscopy (TEM), Fourier transform infrared spectroscopy, and X-ray diffraction. The cytotoxicity of different concentrations of dyed nanoparticles was investigated using (3-(4,5-trihydroxybenzoic acid, dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and flow cytometry. TEM images showed that the average particle sizes of FITC-MSNs and AP-FITC-MSNs were about 100 and 110 nm, respectively. These nanoparticles were internalised by Caco-2 cells, accumulated and dispersed into the cytoplasm, nucleus, and subcellular organelles. Nanoparticles containing GA clearly decreased the viability of cells. FITC-MSNs showed no toxicity on Caco-2 cells at concentrations of ≤50 µg/ml. Functionalisation of FITC-MSNs using APTES decreased toxicity effects on the cells. It was found that FITC-MSNs can be applied at low concentrations as a marker in the cells. In addition, AP-FITC-MSNs showed better biocompatibility with Caco-2 cells than FITC-MSNs, because of their positive surface charges.
2012 19th Iranian Conference of Biomedical Engineering (ICBME), 2012
Intelligent thermosensitive formulations based on PLGA-PEG-PLGA triblock copolymer have great pot... more Intelligent thermosensitive formulations based on PLGA-PEG-PLGA triblock copolymer have great potential in biomedical applications, due to their good biocompatibility, biodegradability and injectability. The gelation behavior of the synthesized PLGA-PEG-PLGA triblocks was investigated in this study and the effect of PEG molecular weight and lactide to glycolide molar ratio, as well as the reaction time was investigated. Triblocks with PEG1000 have shown inappropriate gelation; while those with higher molecular weight formed gel at body temperature. The insulin release profiles showed an approximately linear release profile without a non-desirable burst effect More than 80% of the insulin was released during 4 weeks.
Abstract Gelation time and degradation rate of thermally-sensitive aqueous solutions of chitosan/... more Abstract Gelation time and degradation rate of thermally-sensitive aqueous solutions of chitosan/Gp (glycerophosphate disodium salt) have been studied. The effects of different parameters such as Gp salt concentration, solution temperature, degree of deacetylation ...
Biomedical Engineering Group, Chemical Engineering Faculty, Tarbiat Modares University, Tehran, I... more Biomedical Engineering Group, Chemical Engineering Faculty, Tarbiat Modares University, Tehran, Iran, P.O. Box: 14155-143 Abstract Mucoadhesive polymers were first used in the early 1980s to design mucoadhesive buccal drug delivery systems. These systems have been introduced as novel drug delivery systems due to the ability to stick and stay on mucus membranes and slow release of the drug, and are a suitable alternative to overcome the problems of common oral drug delivery systems. Mucoadhesive systems can be prepared in various forms, such as tablet, gel, oral film, spray, viscose solution and micro/ nanoparticle, and can be used for drug delivery from the buccal, sublingual, intestine, ocular, nasal, vaginal, and other mucosal routes. Mucoadhesive polymers wich are the most important component of these drug delivery systems, are usually natural, synthetic or seminatural hydrophilic macro-molecules, containing functional groups forming hydrogen bonds, such as carboxyl and hydroxyl ...
Key Words chitosan properties, chitosan microparticles, drug delivery, spray drying, ionic gelati... more Key Words chitosan properties, chitosan microparticles, drug delivery, spray drying, ionic gelation Polymerization Quarterly, 2016 T he development of a new drug molecule is an expensive and time consuming effort. Improving the safety efficacy ratio of old drugs has been attempted by different methods focused on each individual drug therapy, dose specification and therapeutic drug monitoring. Controlled rate delivery, slow delivery and targeted delivery are other very promising methods which have been pursued intensively. Methods for increasing bioavailability, diminishing side-effects, promoting targeted delivery and treating conditions such as blood-brain barrier disorders, are the main topics in novel drug delivery explorations. Choosing a suitable polymer is one of the most important aspects in development of a drug delivery system. In the last three decades, avast number of biomedical applications have been reported for a chitin derivative, known as chitosan. In this overview t...
Uploads
Papers by Fariba Ganji