Electron-rich, nitrogenous heteroaromatic compounds interact more with biological/cellular compon... more Electron-rich, nitrogenous heteroaromatic compounds interact more with biological/cellular components than their non-nitrogenous counterparts. The strong intermolecular interactions with proteins, enzymes, and receptors confer significant biological and therapeutic properties to the imidazole derivatives, giving rise to a well-known and extensively used range of therapeutic drugs used for infections, inflammation, and cancer, to name a few. The current study investigates the anti-cancer properties of fourteen previously synthesized nitrogenous heterocycles, derivatives of imidazole and oxazolone, on a panel of cancer cell lines and, in addition, predicts the molecular interactions, pharmacokinetic and safety profiles of these compounds. Method: The MTT and CellTiter-Glo® assays were used to screen the imidazole and oxazolone derivatives on six cancer cell lines: HL60, MDA-MB-321, KAIMRC1, KMIRC2, MCF-10A, and HCT8. Subsequently, in vitro tubulin staining and imaging were performed, ...
Liver cancer is the third leading cause of cancer death worldwide. Marine mollusc-derived extract... more Liver cancer is the third leading cause of cancer death worldwide. Marine mollusc-derived extracts have gained attention as new potential natural-based anticancer agents to overcome the side effects caused by conventional chemotherapeutic drugs during cancer therapy. We evaluated the cytotoxic effects of a crude extract from the purple-ink released by the sea hare named Bursatella leachii (B. leachii) against human hepatocarcinoma cell line (HepG2) and explored the underlying mechanisms causing the programmed cell death (i.e., apoptosis). Expression of cleaved-caspase-8 and cleaved-caspase-3, key cysteine-aspartic proteases involved in the initiation and completion of the apoptosis process, appeared after HepG2 cell exposure to B. leachii extract. Gene expression levels of pro-apoptotic BAX, tumour suppressor TP53 and Cyclin D1 were increased after treatment with B. leachii. Using liquid chromatography-mass spectrometry, the main biomolecules in the B. leachii extract were identifie...
Neurodegenerative diseases (NDs) are one of the most challenging public health issues. Despite tr... more Neurodegenerative diseases (NDs) are one of the most challenging public health issues. Despite tremendous advances in our understanding of NDs, little progress has been made in establishing effective treatments. Natural products may have enormous potential in preventing and treating NDs by targeting microglia; yet, there have been several clinical concerns about their usage, primarily due to a lack of scientific evidence for their efficacy, molecular targets, physicochemical properties, and safety. To solve this problem, the secondary bioactive metabolites derived from neuroprotective medicinal plants were identified and selected for computational predictions for anti-inflammatory activity, possible molecular targets, physicochemical properties, and safety evaluation using PASS online, Molinspiration, SwissADME, and ProTox-II, respectively. Most of the phytochemicals were active as anti-inflammatory agents as predicted using the PASS online webserver. Moreover, the molecular target ...
Objectives: To prepare tablets by direct compression method using natural gums such as acacia (F1... more Objectives: To prepare tablets by direct compression method using natural gums such as acacia (F1, F2 and F3), gelatin (F4, F5 and F6) and tragacanth (F7, F8 and F9) at different concentrations. To assess the flow properties of the powder blend (polymers mixed with the drug). To evaluate physical properties of the polymer matrix based tablets for weight variation, hardness, friability, thickness, drug content uniformity, disintegration time, swelling index and in-vitro drug release. To perform stability studies for the optimized formula. Method: In the current investigation, an attempt was done to formulate sustained release matrix tablets using natural polymers, acacia, gelatin and tragacanth as release modifier. Paracetamol was used as a model drug. The different ratios used drug: Lactose: polymer were 100: 200: 50, 100: 150: 100 and 100: 100: 150 for acacia, gelatin and tragacanth respectively. Tablets were prepared by direct compression and evaluated by powder characterization, ...
Liver cancer is a leading cause of cancer death globally. Marine mollusc-derived drugs have gaine... more Liver cancer is a leading cause of cancer death globally. Marine mollusc-derived drugs have gained attention as potential natural-based anti-cancer agents to overcome the side effects caused by conventional chemotherapeutic drugs during cancer therapy. Using liquid chromatography-mass spectrometry, the main biomolecules in the purple ink secretion released by the sea hare, named Bursatella leachii (B. leachii), were identified as hectochlorin, malyngamide X, malyngolide S, bursatellin and lyngbyatoxin A. The cytotoxic effects of B. leachii ink concentrate against human hepatocarcinoma (HepG2) cells were determined to be dose- and time-dependent, and further exploration of the underlying mechanisms causing the programmed cell death (apoptosis) were performed. The expression of cleaved-caspase-8 and cleaved-caspase-3, key cysteine-aspartic proteases involved in the initiation and completion of the apoptosis process, appeared after HepG2 cell exposure to the B. leachii ink concentrate....
Herbal medicine has a long history of being used for treating various ailments ranging in severit... more Herbal medicine has a long history of being used for treating various ailments ranging in severity. The growing popularity of herbal medicine, termed the green boom, may be ascribed to people taking a more holistic approach to maintain their health.Traditional medicine is widely used on a regular basis by Malaysians .In this study three medicinal plants (Piper Sarmentosum, MoringaOleifera, MurrayaKoenigii) that are traditionally used in the Malaysians for treating infections, were collected for investigation. The bacteria used in this investigation are both Gram-positive and Gramnegative bacteria which is S. aureus, E.coli, P. aeruinosa and Bacillus Subtilis. The microtitre well plate and disc diffusion assay were used for determining the antibacterial activity of the traditional preparations and plants extracts against the different bacterial strains.Themicrotitre well plate was used for testing the antibacterial activity of the plants against the previously mentioned bacterial str...
Nowadays very few drugs are formulated as powders or granules, they are usually taken with some w... more Nowadays very few drugs are formulated as powders or granules, they are usually taken with some water in which they are readily dispersible in & somewhat rapidly dissolving in as a result of the large surface area provided by the small particles of the powder as well as high solubilitys of the other ingredients used in the formulation, leading to high drug absorption, their bioavailability could be similar to the suspensions form in general. This review covers the classifications of pharmaceutical powders, and recent advancements in granulation techniques with their desirable characteristics in enhancing drug bioavailability and manufacturing performance.
Pharmaceutical industries focus on enhancing patients’ compliance by reducing dosing frequency an... more Pharmaceutical industries focus on enhancing patients’ compliance by reducing dosing frequency and minimizing side effects. People above 40s year are frequently experiencing potential drug-drug interaction during combination therapy poly-therapy of oncedaily formulations. The market is progressing rapidly in modified release technological advancements in improving bioavailability of drug delivery systems by controlling the erratic drug-release and plasmaconcentration profiles. Consequently, enhancing patient compliance, cost effective and minimizing side effects and toxicities. This review focusing on the classification of different modified release drug dosage forms with their plasmaconcentration profiles associated with absorption and changes in controlling and management of diseases.
International Journal of Medical Toxicology & Legal Medicine, 2019
Skin whitening body lotions are the cosmetics products which work on human skin by reducing a pig... more Skin whitening body lotions are the cosmetics products which work on human skin by reducing a pigment called melanin in the skin. It also lightens naturally dark skin and dark spots. It is giving a moisturizing effect to the skin as a natural cosmetics body lotion. This study was aimed on a reduction of skin melanin by tyrosine inhibitory with plant extracts by formulating a stable whitening body lotion containing the plant extracts of Psidium guajava L. leaves and also the extract of Crocus sativus (Saffron). Two plants were extracted in a different way; the method of extraction for Crocus sativus was reflux method and for Psidium guajava leaves was maceration method. The phytochemical screening and tyrosinase inhibition activity of extracts were evaluated. The various types of lotion namely F1 to F5 were formulated by incorporating different concentrations of extracts and evaluated for different parameters. The findings of tyrosinase inhibitory study exhibited that the IC50 value of tyrosine inhibitory of combined extract of Psidium guajava and Crocus sativus was lower compared to Crocus sativus extract. The formulation F3 with the mixture 0.5 g of Crocus sativus and 0.5 g of Psidium guajava leaves showed the best results among several formulations. The result which obtained from formulation F3 was compatible to pass all the evaluation tests. As a conclusion, the mixture of Psidium guajava and Crocus sativus extract possesses a strong tyrosine inhibition activity, and it was formulated as a stable whitening natural body lotion, and it can be safely used on the skin.
... CONCENTRATION (MIC) OF SOME NOVEL TRIAZOLE DERIVATIVE Arun Kumar Wahi1, Arti Singh2* and Ajit... more ... CONCENTRATION (MIC) OF SOME NOVEL TRIAZOLE DERIVATIVE Arun Kumar Wahi1, Arti Singh2* and Ajit Kumar Singh3 ... 2008;43:1989-1996. 8. Shiradkar MR, Ghodake M, Bothara KG, Bhandari SV, Nikalje A, Akula KC, Desai NC, and Burange PJ. ...
Antioxidants can prevent or slow the oxidative damage to our body cells. This study was conducted... more Antioxidants can prevent or slow the oxidative damage to our body cells. This study was conducted in order to determine if there is any relationship between electrical current conductivity and antioxidant property of the ten Malaysian medicinal plants. The objective of this study was to measure the antioxidant property of the herbal extracts, as well as to measure the ability of the herbal extracts to conduct an electrical current. Ten herbs were selected randomly, Strobilaunthus crispa, Pereskia sacharosa, Ehretia laevis, Plectranthus ambinicus, Orthosiphon staminous, Androghophis panculata, Persicaria odorata, Clinacantus nutans, Curry leaves and Frankincense. They were identified and prepared for extraction. The methanol extracts were obtained by ultrasonification extraction method. The antioxidant property was carried out using 1, 1-diphenyl-2-picrylhydrazy (DPPH) reagent. The pH was measured using a pH meter and the phytochemical elements were also tested. Finally the electrica...
International Journal of Pharmacognosy and Phytochemical Research
This study was conducted in order to determine if the herbal extract having antioxidant property.... more This study was conducted in order to determine if the herbal extract having antioxidant property. The major princible is to find out if there is any relationship between electrical current conductivity and antioxidant property of the ten Malaysian medicinal plants. The objective of this study achieved by measuring the antioxidant property of the herbal extracts, as well as to measure the ability of the herbal extracts to conduct an electrical current. Ten herbs were selected randomly, Strobilaunthus crispa, Pereskia sacharosa, Ehretia laevis, Plectranthus ambinicus, Orthosiphon staminous, Androghophis panculata, Persicaria odorata, Clinacantus nutans, Curry leaves and Frankincense. They were identified and prepared for extraction. The methanol extracts were obtained by ultrasonification extraction method. The antioxidant property was carried out using 1, 1-diphenyl-2-picrylhydrazy (DPPH) reagent. The pH was measured using a pH meter and the phytochemical elements were also tested. F...
Objective: Three furosemide aqueous suspensions for paediatric oral usage (2 mg/ml) were investig... more Objective: Three furosemide aqueous suspensions for paediatric oral usage (2 mg/ml) were investigated to determinate its physicochemical stability under different storage conditions. Method: Formulations using sugar syrup and sugar-free vehicles were stored at 4 and 25 o C and the furosemide content was determined by spectrophotometer. Each sample was analyzed in triplicate at different time points (0, 7, 14, 28, 56, 72 and 90 days). Results: Liquid suspensions were successfully formulated from commercially available tablets. In both cases, samples showed suitable physical stability. Furosemide was chemically stable in aqueous suspension during the 90 days of the study at the two storage temperatures. Conclusions: All the tried oral liquid formulations can be conserved at 4 and 25 o C at least 56, 72 and 90 day period.
Different extraction techniques can produce extracts of divergent active compounds from plants. P... more Different extraction techniques can produce extracts of divergent active compounds from plants. Percolation with soxhlet and ultrasonication were used with ethanol solvent to extract hibiscus and jasmine flowers. These extracts were tested for antibacterial effect on Escherichia coli and Staphylococcus aureus with susceptibility test. The study was conducted by extracting the flowers with soxhlet and ultrasonic apparatus using ethanol as solvent. The extracts were separated from the solvent by evaporating the solvent with rotary evaporator. Discs of 6 mm were prepared from filter paper and were impregnated with the extracts. Sub-cultured test organisms, E. coli and S. aureus were swabbed on Mueller Hinton (MH) agars for susceptibility testing. The prepared discs were placed on the swabbed MH agar plates. Commercially prepared jasmine oil and hibiscus tea extracts’ discs were used as standards. Gentamicin discs were used as positive control and ethanol impregnated discs were used as ...
International Journal of Scientific & Technology Research, 2015
The use of digital imaging for the diagnostic purpose has been increased during last few years. T... more The use of digital imaging for the diagnostic purpose has been increased during last few years. This technique has brought advancement in the field of radiography. The effect of digital imaging on workflow has a direct relation with the performance of technologists. Aim: The predestined aim of this study was to analyze the use and influence of digital imaging on the workflow of the radiologists and also to demonstrate the effects on the overall performance of healthcare delivery. Method: A quantitative method of analysis is used in this study and the study instrument consisted of a questionnaire. The questions relates to the effect of digital imaging on workflow and image quality. Total of100 questionnaires were distributed, and 82 of them were retrieved back. The inclusion criteria include healthcare providers who are radiographers, radiologist and radiology nurses. The healthcare providers of all ages, gender, and ethnicity, were included in this study. Results: according to 70.2%...
The advent of new chemical genetic tools and high-throughput screening technologies and platforms... more The advent of new chemical genetic tools and high-throughput screening technologies and platforms have already led and will continue to lead in accessing the natural product diversity of microorganisms. This thesis represents a continuation of the work on the investigation into means of making better use of the advantages of new technologies and the OSMAC approach (One Strain Many Compounds) in drug discovery that the Atta-ur-Rahman Research Institute of Natural Product Discovery (RiND) at MARA University of Technology has engaged in. The result of this thesis is the MECSUS (Microtiter plate, Elicitors, Combination, Solid phase extraction, UHPLC, Statistical analysis) protocol. It involves miniaturized parallel fermentations in 96-well microtiter plate with up to ninety six different media, parallel extraction of the supernatant layer of the fungus via a polymer based solid phase extraction (SPE) plate, chromatographic assessment of the results via UHPLC, and multivariate analysis o...
Objective: The aim of the present study was to characterize the antimicrobial properties of Acaci... more Objective: The aim of the present study was to characterize the antimicrobial properties of Acacia Gum Arabia crude extracts and Acacia Gum Arabia aqueous cream against certain selected quality control microorganisms. Methodology: A serial concentrations were prepared 2.5%, 5%, 10%, 15%, and 20% of Acacia Gum Arabia. An aqueous cream with 5% concentration of Acacia Gum Arabia were prepared and evaluated. A quality control tests were conducted to check the stability, organoleptic properties and pH of the cream. An in vitro evaluation against Gram positive bacteria (Bacillus subtilis and Staphylococcus aureus), Gram negative bacteria (Escherichia coli, and Pseudomonas aeroginosa) and yeast (Candida albicans). The Minimum Inhibitory Concentration (MIC) were determined. Results: Out of sex (6) tested microorganisms, it is found that 5% is the optimum concentration that most of the microorganisms are reported to shows zone of inhibitions. A further experiments were conducted for minimum ...
Electron-rich, nitrogenous heteroaromatic compounds interact more with biological/cellular compon... more Electron-rich, nitrogenous heteroaromatic compounds interact more with biological/cellular components than their non-nitrogenous counterparts. The strong intermolecular interactions with proteins, enzymes, and receptors confer significant biological and therapeutic properties to the imidazole derivatives, giving rise to a well-known and extensively used range of therapeutic drugs used for infections, inflammation, and cancer, to name a few. The current study investigates the anti-cancer properties of fourteen previously synthesized nitrogenous heterocycles, derivatives of imidazole and oxazolone, on a panel of cancer cell lines and, in addition, predicts the molecular interactions, pharmacokinetic and safety profiles of these compounds. Method: The MTT and CellTiter-Glo® assays were used to screen the imidazole and oxazolone derivatives on six cancer cell lines: HL60, MDA-MB-321, KAIMRC1, KMIRC2, MCF-10A, and HCT8. Subsequently, in vitro tubulin staining and imaging were performed, ...
Liver cancer is the third leading cause of cancer death worldwide. Marine mollusc-derived extract... more Liver cancer is the third leading cause of cancer death worldwide. Marine mollusc-derived extracts have gained attention as new potential natural-based anticancer agents to overcome the side effects caused by conventional chemotherapeutic drugs during cancer therapy. We evaluated the cytotoxic effects of a crude extract from the purple-ink released by the sea hare named Bursatella leachii (B. leachii) against human hepatocarcinoma cell line (HepG2) and explored the underlying mechanisms causing the programmed cell death (i.e., apoptosis). Expression of cleaved-caspase-8 and cleaved-caspase-3, key cysteine-aspartic proteases involved in the initiation and completion of the apoptosis process, appeared after HepG2 cell exposure to B. leachii extract. Gene expression levels of pro-apoptotic BAX, tumour suppressor TP53 and Cyclin D1 were increased after treatment with B. leachii. Using liquid chromatography-mass spectrometry, the main biomolecules in the B. leachii extract were identifie...
Neurodegenerative diseases (NDs) are one of the most challenging public health issues. Despite tr... more Neurodegenerative diseases (NDs) are one of the most challenging public health issues. Despite tremendous advances in our understanding of NDs, little progress has been made in establishing effective treatments. Natural products may have enormous potential in preventing and treating NDs by targeting microglia; yet, there have been several clinical concerns about their usage, primarily due to a lack of scientific evidence for their efficacy, molecular targets, physicochemical properties, and safety. To solve this problem, the secondary bioactive metabolites derived from neuroprotective medicinal plants were identified and selected for computational predictions for anti-inflammatory activity, possible molecular targets, physicochemical properties, and safety evaluation using PASS online, Molinspiration, SwissADME, and ProTox-II, respectively. Most of the phytochemicals were active as anti-inflammatory agents as predicted using the PASS online webserver. Moreover, the molecular target ...
Objectives: To prepare tablets by direct compression method using natural gums such as acacia (F1... more Objectives: To prepare tablets by direct compression method using natural gums such as acacia (F1, F2 and F3), gelatin (F4, F5 and F6) and tragacanth (F7, F8 and F9) at different concentrations. To assess the flow properties of the powder blend (polymers mixed with the drug). To evaluate physical properties of the polymer matrix based tablets for weight variation, hardness, friability, thickness, drug content uniformity, disintegration time, swelling index and in-vitro drug release. To perform stability studies for the optimized formula. Method: In the current investigation, an attempt was done to formulate sustained release matrix tablets using natural polymers, acacia, gelatin and tragacanth as release modifier. Paracetamol was used as a model drug. The different ratios used drug: Lactose: polymer were 100: 200: 50, 100: 150: 100 and 100: 100: 150 for acacia, gelatin and tragacanth respectively. Tablets were prepared by direct compression and evaluated by powder characterization, ...
Liver cancer is a leading cause of cancer death globally. Marine mollusc-derived drugs have gaine... more Liver cancer is a leading cause of cancer death globally. Marine mollusc-derived drugs have gained attention as potential natural-based anti-cancer agents to overcome the side effects caused by conventional chemotherapeutic drugs during cancer therapy. Using liquid chromatography-mass spectrometry, the main biomolecules in the purple ink secretion released by the sea hare, named Bursatella leachii (B. leachii), were identified as hectochlorin, malyngamide X, malyngolide S, bursatellin and lyngbyatoxin A. The cytotoxic effects of B. leachii ink concentrate against human hepatocarcinoma (HepG2) cells were determined to be dose- and time-dependent, and further exploration of the underlying mechanisms causing the programmed cell death (apoptosis) were performed. The expression of cleaved-caspase-8 and cleaved-caspase-3, key cysteine-aspartic proteases involved in the initiation and completion of the apoptosis process, appeared after HepG2 cell exposure to the B. leachii ink concentrate....
Herbal medicine has a long history of being used for treating various ailments ranging in severit... more Herbal medicine has a long history of being used for treating various ailments ranging in severity. The growing popularity of herbal medicine, termed the green boom, may be ascribed to people taking a more holistic approach to maintain their health.Traditional medicine is widely used on a regular basis by Malaysians .In this study three medicinal plants (Piper Sarmentosum, MoringaOleifera, MurrayaKoenigii) that are traditionally used in the Malaysians for treating infections, were collected for investigation. The bacteria used in this investigation are both Gram-positive and Gramnegative bacteria which is S. aureus, E.coli, P. aeruinosa and Bacillus Subtilis. The microtitre well plate and disc diffusion assay were used for determining the antibacterial activity of the traditional preparations and plants extracts against the different bacterial strains.Themicrotitre well plate was used for testing the antibacterial activity of the plants against the previously mentioned bacterial str...
Nowadays very few drugs are formulated as powders or granules, they are usually taken with some w... more Nowadays very few drugs are formulated as powders or granules, they are usually taken with some water in which they are readily dispersible in & somewhat rapidly dissolving in as a result of the large surface area provided by the small particles of the powder as well as high solubilitys of the other ingredients used in the formulation, leading to high drug absorption, their bioavailability could be similar to the suspensions form in general. This review covers the classifications of pharmaceutical powders, and recent advancements in granulation techniques with their desirable characteristics in enhancing drug bioavailability and manufacturing performance.
Pharmaceutical industries focus on enhancing patients’ compliance by reducing dosing frequency an... more Pharmaceutical industries focus on enhancing patients’ compliance by reducing dosing frequency and minimizing side effects. People above 40s year are frequently experiencing potential drug-drug interaction during combination therapy poly-therapy of oncedaily formulations. The market is progressing rapidly in modified release technological advancements in improving bioavailability of drug delivery systems by controlling the erratic drug-release and plasmaconcentration profiles. Consequently, enhancing patient compliance, cost effective and minimizing side effects and toxicities. This review focusing on the classification of different modified release drug dosage forms with their plasmaconcentration profiles associated with absorption and changes in controlling and management of diseases.
International Journal of Medical Toxicology & Legal Medicine, 2019
Skin whitening body lotions are the cosmetics products which work on human skin by reducing a pig... more Skin whitening body lotions are the cosmetics products which work on human skin by reducing a pigment called melanin in the skin. It also lightens naturally dark skin and dark spots. It is giving a moisturizing effect to the skin as a natural cosmetics body lotion. This study was aimed on a reduction of skin melanin by tyrosine inhibitory with plant extracts by formulating a stable whitening body lotion containing the plant extracts of Psidium guajava L. leaves and also the extract of Crocus sativus (Saffron). Two plants were extracted in a different way; the method of extraction for Crocus sativus was reflux method and for Psidium guajava leaves was maceration method. The phytochemical screening and tyrosinase inhibition activity of extracts were evaluated. The various types of lotion namely F1 to F5 were formulated by incorporating different concentrations of extracts and evaluated for different parameters. The findings of tyrosinase inhibitory study exhibited that the IC50 value of tyrosine inhibitory of combined extract of Psidium guajava and Crocus sativus was lower compared to Crocus sativus extract. The formulation F3 with the mixture 0.5 g of Crocus sativus and 0.5 g of Psidium guajava leaves showed the best results among several formulations. The result which obtained from formulation F3 was compatible to pass all the evaluation tests. As a conclusion, the mixture of Psidium guajava and Crocus sativus extract possesses a strong tyrosine inhibition activity, and it was formulated as a stable whitening natural body lotion, and it can be safely used on the skin.
... CONCENTRATION (MIC) OF SOME NOVEL TRIAZOLE DERIVATIVE Arun Kumar Wahi1, Arti Singh2* and Ajit... more ... CONCENTRATION (MIC) OF SOME NOVEL TRIAZOLE DERIVATIVE Arun Kumar Wahi1, Arti Singh2* and Ajit Kumar Singh3 ... 2008;43:1989-1996. 8. Shiradkar MR, Ghodake M, Bothara KG, Bhandari SV, Nikalje A, Akula KC, Desai NC, and Burange PJ. ...
Antioxidants can prevent or slow the oxidative damage to our body cells. This study was conducted... more Antioxidants can prevent or slow the oxidative damage to our body cells. This study was conducted in order to determine if there is any relationship between electrical current conductivity and antioxidant property of the ten Malaysian medicinal plants. The objective of this study was to measure the antioxidant property of the herbal extracts, as well as to measure the ability of the herbal extracts to conduct an electrical current. Ten herbs were selected randomly, Strobilaunthus crispa, Pereskia sacharosa, Ehretia laevis, Plectranthus ambinicus, Orthosiphon staminous, Androghophis panculata, Persicaria odorata, Clinacantus nutans, Curry leaves and Frankincense. They were identified and prepared for extraction. The methanol extracts were obtained by ultrasonification extraction method. The antioxidant property was carried out using 1, 1-diphenyl-2-picrylhydrazy (DPPH) reagent. The pH was measured using a pH meter and the phytochemical elements were also tested. Finally the electrica...
International Journal of Pharmacognosy and Phytochemical Research
This study was conducted in order to determine if the herbal extract having antioxidant property.... more This study was conducted in order to determine if the herbal extract having antioxidant property. The major princible is to find out if there is any relationship between electrical current conductivity and antioxidant property of the ten Malaysian medicinal plants. The objective of this study achieved by measuring the antioxidant property of the herbal extracts, as well as to measure the ability of the herbal extracts to conduct an electrical current. Ten herbs were selected randomly, Strobilaunthus crispa, Pereskia sacharosa, Ehretia laevis, Plectranthus ambinicus, Orthosiphon staminous, Androghophis panculata, Persicaria odorata, Clinacantus nutans, Curry leaves and Frankincense. They were identified and prepared for extraction. The methanol extracts were obtained by ultrasonification extraction method. The antioxidant property was carried out using 1, 1-diphenyl-2-picrylhydrazy (DPPH) reagent. The pH was measured using a pH meter and the phytochemical elements were also tested. F...
Objective: Three furosemide aqueous suspensions for paediatric oral usage (2 mg/ml) were investig... more Objective: Three furosemide aqueous suspensions for paediatric oral usage (2 mg/ml) were investigated to determinate its physicochemical stability under different storage conditions. Method: Formulations using sugar syrup and sugar-free vehicles were stored at 4 and 25 o C and the furosemide content was determined by spectrophotometer. Each sample was analyzed in triplicate at different time points (0, 7, 14, 28, 56, 72 and 90 days). Results: Liquid suspensions were successfully formulated from commercially available tablets. In both cases, samples showed suitable physical stability. Furosemide was chemically stable in aqueous suspension during the 90 days of the study at the two storage temperatures. Conclusions: All the tried oral liquid formulations can be conserved at 4 and 25 o C at least 56, 72 and 90 day period.
Different extraction techniques can produce extracts of divergent active compounds from plants. P... more Different extraction techniques can produce extracts of divergent active compounds from plants. Percolation with soxhlet and ultrasonication were used with ethanol solvent to extract hibiscus and jasmine flowers. These extracts were tested for antibacterial effect on Escherichia coli and Staphylococcus aureus with susceptibility test. The study was conducted by extracting the flowers with soxhlet and ultrasonic apparatus using ethanol as solvent. The extracts were separated from the solvent by evaporating the solvent with rotary evaporator. Discs of 6 mm were prepared from filter paper and were impregnated with the extracts. Sub-cultured test organisms, E. coli and S. aureus were swabbed on Mueller Hinton (MH) agars for susceptibility testing. The prepared discs were placed on the swabbed MH agar plates. Commercially prepared jasmine oil and hibiscus tea extracts’ discs were used as standards. Gentamicin discs were used as positive control and ethanol impregnated discs were used as ...
International Journal of Scientific & Technology Research, 2015
The use of digital imaging for the diagnostic purpose has been increased during last few years. T... more The use of digital imaging for the diagnostic purpose has been increased during last few years. This technique has brought advancement in the field of radiography. The effect of digital imaging on workflow has a direct relation with the performance of technologists. Aim: The predestined aim of this study was to analyze the use and influence of digital imaging on the workflow of the radiologists and also to demonstrate the effects on the overall performance of healthcare delivery. Method: A quantitative method of analysis is used in this study and the study instrument consisted of a questionnaire. The questions relates to the effect of digital imaging on workflow and image quality. Total of100 questionnaires were distributed, and 82 of them were retrieved back. The inclusion criteria include healthcare providers who are radiographers, radiologist and radiology nurses. The healthcare providers of all ages, gender, and ethnicity, were included in this study. Results: according to 70.2%...
The advent of new chemical genetic tools and high-throughput screening technologies and platforms... more The advent of new chemical genetic tools and high-throughput screening technologies and platforms have already led and will continue to lead in accessing the natural product diversity of microorganisms. This thesis represents a continuation of the work on the investigation into means of making better use of the advantages of new technologies and the OSMAC approach (One Strain Many Compounds) in drug discovery that the Atta-ur-Rahman Research Institute of Natural Product Discovery (RiND) at MARA University of Technology has engaged in. The result of this thesis is the MECSUS (Microtiter plate, Elicitors, Combination, Solid phase extraction, UHPLC, Statistical analysis) protocol. It involves miniaturized parallel fermentations in 96-well microtiter plate with up to ninety six different media, parallel extraction of the supernatant layer of the fungus via a polymer based solid phase extraction (SPE) plate, chromatographic assessment of the results via UHPLC, and multivariate analysis o...
Objective: The aim of the present study was to characterize the antimicrobial properties of Acaci... more Objective: The aim of the present study was to characterize the antimicrobial properties of Acacia Gum Arabia crude extracts and Acacia Gum Arabia aqueous cream against certain selected quality control microorganisms. Methodology: A serial concentrations were prepared 2.5%, 5%, 10%, 15%, and 20% of Acacia Gum Arabia. An aqueous cream with 5% concentration of Acacia Gum Arabia were prepared and evaluated. A quality control tests were conducted to check the stability, organoleptic properties and pH of the cream. An in vitro evaluation against Gram positive bacteria (Bacillus subtilis and Staphylococcus aureus), Gram negative bacteria (Escherichia coli, and Pseudomonas aeroginosa) and yeast (Candida albicans). The Minimum Inhibitory Concentration (MIC) were determined. Results: Out of sex (6) tested microorganisms, it is found that 5% is the optimum concentration that most of the microorganisms are reported to shows zone of inhibitions. A further experiments were conducted for minimum ...
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Papers by Rasha S Suliman