Background: Microalgae due to an array of its antioxidant, antimicrobial and anti-inflammatory bi... more Background: Microalgae due to an array of its antioxidant, antimicrobial and anti-inflammatory bioactives have been changing the cosmeceutical market. One such bioactive power pack is Chlorella emersonii KJ725233, a novel, non-fastidious freshwater microalga with an inherently higher anti-oxidant potential. Objective: The present study evaluates its potential use as a cosmeceutical ingredient with arrays of biochemical assays, toxicity studies and GC-HRMS profiling. Materials and Methods: In vitro protein-ase inhibition activity of the methanolic extract of C.emersonii KJ725233 was evaluated using SAAANA, sodium hyaluronate and bovine collagen as substrates respectively. The radical scavenging potential was determined by DPPH assay and was correlated to the total flavonoid content evaluated by aluminium trichloride method. The bioactive composition was evaluated by GC – HRMS analysis. Eye irritation potential and sulforhodamine B assay were carried out using 3T3 fibroblasts cells to ascertain its non-toxic nature. Results: The methanolic extract of C.emersonii KJ725233 exhibited a potent elastase inhibition of 92.04±2.27% at a concentration of 1000 µg/mL. This was qualitatively established by the presence of phytol, its
Phycoremediation is a process that uses microalgae for water remediation. The limitations to the ... more Phycoremediation is a process that uses microalgae for water remediation. The limitations to the slow growth rate and low biomass of plants in case of phytoremediation can be effectively managed by Phycoremediation. The present study efficiently couples remediation of nitrate, phosphate levels in a fresh water source with single cell protein production. The Spirulina-Chlorella consortia was tested for its
remediation efficacy in the form of a floating wetland for a maximum period of 10 days. The results were promising with a reduction of 34.36 ± 1.19 % of nitrates, 83.39 ± 0.66 % of phosphates and 97.79 ± 0.79% in bacterial load (cfu/mL). The consortia thus reared was found to have 79 ± 0.2 % protein, 19.8 ± 0.34 % lipids and 9 ± 0.42 % carbohydrates making it a prospective feed supplement. This pilot scale set up thus seems to be a promising solution for onsite remediation of agricultural run-offs that are rich in
nitrates and phosphates.
Phycoremediation is a process that uses microalgae for water remediation. The limitations to the ... more Phycoremediation is a process that uses microalgae for water remediation. The limitations to the slow growth rate and low biomass of plants in case of phytoremediation can be effectively managed by Phycoremediation. The present study efficiently couples remediation of nitrate, phosphate levels in a fresh water source with single cell protein production. The Spirulina-Chlorella consortia was tested for its remediation efficacy in the form of a floating wetland for a maximum period of 10 days. The results were promising with a reduction of 34.36 ± 1.19 % of nitrates, 83.39 ± 0.66 % of phosphates and 97.79 ± 0.79% in bacterial load (cfu/mL). The consortia thus reared was found to have 79 ± 0.2 % protein, 19.8 ± 0.34 % lipids and 9 ± 0.42 % carbohydrates making it a prospective feed supplement. This pilot scale set up thus seems to be a promising solution for onsite remediation of agricultural runoffs that are rich in nitrates and phosphates.
Objective: At low concentrations copper is an essential micronutrient for algal growth wherein it... more Objective: At low concentrations copper is an essential micronutrient for algal growth wherein it plays a vital role as an enzyme cofactor for photosynthetic processes but at high concentrations it functions as a toxic heavy metal. Copper exposure to microalga increases the activity of antioxidative enzymes–catalase, superoxide dismutase, peroxidase along with the induction of chlorosis at higher concentrations. The objective of the present study is to determine the effect of suboptimal concentration of copper on the generation time as well as the antioxidant potential of a novel strain-Chlorella emersonii KJ725233. Methods: The growth of the microalga at the different copper concentrations was monitored by measuring the absorbance at 684 nm. Once the suboptimum copper concentration for the growth of the microalga was determined, the methanolic extracts were evaluated for Total Phenolic Content, Total Flavonoid Contents as well as the antioxidant potential by employing standard methods such as phospho-molybdenum method for Total Antioxidant Capacity and Ferric Reducing Antioxidant Potential. Results: Results indicated that 0.1 µmol of copper stimulated the growth of Chlorella emersonii KJ725233, which was evident from the reduced generation time of the microalga as compared to that of the control and other copper concentrations. The study also proved a 45.69% increase in the antioxidant activity of the microalga on exposure to 0.1 µmol of copper. Conclusion: The present study indicated copper at 0.1 µmol concentration not only acts as a micronutrient but also levies stress resulting in increased antioxidant activity of this novel isolate Chlorella emersonii KJ725233.
Background: Microalgae due to an array of its antioxidant, antimicrobial and anti-inflammatory bi... more Background: Microalgae due to an array of its antioxidant, antimicrobial and anti-inflammatory bioactives have been changing the cosmeceutical market. One such bioactive power pack is Chlorella emersonii KJ725233, a novel, non-fastidious freshwater microalga with an inherently higher anti-oxidant potential. Objective: The present study evaluates its potential use as a cosmeceutical ingredient with arrays of biochemical assays, toxicity studies and GC-HRMS profiling. Materials and Methods: In vitro protein-ase inhibition activity of the methanolic extract of C.emersonii KJ725233 was evaluated using SAAANA, sodium hyaluronate and bovine collagen as substrates respectively. The radical scavenging potential was determined by DPPH assay and was correlated to the total flavonoid content evaluated by aluminium trichloride method. The bioactive composition was evaluated by GC – HRMS analysis. Eye irritation potential and sulforhodamine B assay were carried out using 3T3 fibroblasts cells to ascertain its non-toxic nature. Results: The methanolic extract of C.emersonii KJ725233 exhibited a potent elastase inhibition of 92.04±2.27% at a concentration of 1000 µg/mL. This was qualitatively established by the presence of phytol, its
Phycoremediation is a process that uses microalgae for water remediation. The limitations to the ... more Phycoremediation is a process that uses microalgae for water remediation. The limitations to the slow growth rate and low biomass of plants in case of phytoremediation can be effectively managed by Phycoremediation. The present study efficiently couples remediation of nitrate, phosphate levels in a fresh water source with single cell protein production. The Spirulina-Chlorella consortia was tested for its
remediation efficacy in the form of a floating wetland for a maximum period of 10 days. The results were promising with a reduction of 34.36 ± 1.19 % of nitrates, 83.39 ± 0.66 % of phosphates and 97.79 ± 0.79% in bacterial load (cfu/mL). The consortia thus reared was found to have 79 ± 0.2 % protein, 19.8 ± 0.34 % lipids and 9 ± 0.42 % carbohydrates making it a prospective feed supplement. This pilot scale set up thus seems to be a promising solution for onsite remediation of agricultural run-offs that are rich in
nitrates and phosphates.
Phycoremediation is a process that uses microalgae for water remediation. The limitations to the ... more Phycoremediation is a process that uses microalgae for water remediation. The limitations to the slow growth rate and low biomass of plants in case of phytoremediation can be effectively managed by Phycoremediation. The present study efficiently couples remediation of nitrate, phosphate levels in a fresh water source with single cell protein production. The Spirulina-Chlorella consortia was tested for its remediation efficacy in the form of a floating wetland for a maximum period of 10 days. The results were promising with a reduction of 34.36 ± 1.19 % of nitrates, 83.39 ± 0.66 % of phosphates and 97.79 ± 0.79% in bacterial load (cfu/mL). The consortia thus reared was found to have 79 ± 0.2 % protein, 19.8 ± 0.34 % lipids and 9 ± 0.42 % carbohydrates making it a prospective feed supplement. This pilot scale set up thus seems to be a promising solution for onsite remediation of agricultural runoffs that are rich in nitrates and phosphates.
Objective: At low concentrations copper is an essential micronutrient for algal growth wherein it... more Objective: At low concentrations copper is an essential micronutrient for algal growth wherein it plays a vital role as an enzyme cofactor for photosynthetic processes but at high concentrations it functions as a toxic heavy metal. Copper exposure to microalga increases the activity of antioxidative enzymes–catalase, superoxide dismutase, peroxidase along with the induction of chlorosis at higher concentrations. The objective of the present study is to determine the effect of suboptimal concentration of copper on the generation time as well as the antioxidant potential of a novel strain-Chlorella emersonii KJ725233. Methods: The growth of the microalga at the different copper concentrations was monitored by measuring the absorbance at 684 nm. Once the suboptimum copper concentration for the growth of the microalga was determined, the methanolic extracts were evaluated for Total Phenolic Content, Total Flavonoid Contents as well as the antioxidant potential by employing standard methods such as phospho-molybdenum method for Total Antioxidant Capacity and Ferric Reducing Antioxidant Potential. Results: Results indicated that 0.1 µmol of copper stimulated the growth of Chlorella emersonii KJ725233, which was evident from the reduced generation time of the microalga as compared to that of the control and other copper concentrations. The study also proved a 45.69% increase in the antioxidant activity of the microalga on exposure to 0.1 µmol of copper. Conclusion: The present study indicated copper at 0.1 µmol concentration not only acts as a micronutrient but also levies stress resulting in increased antioxidant activity of this novel isolate Chlorella emersonii KJ725233.
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remediation efficacy in the form of a floating wetland for a maximum period of 10 days. The results were promising with a reduction of 34.36 ± 1.19 % of nitrates, 83.39 ± 0.66 % of phosphates and 97.79 ± 0.79% in bacterial load (cfu/mL). The consortia thus reared was found to have 79 ± 0.2 % protein, 19.8 ± 0.34 % lipids and 9 ± 0.42 % carbohydrates making it a prospective feed supplement. This pilot scale set up thus seems to be a promising solution for onsite remediation of agricultural run-offs that are rich in
nitrates and phosphates.
remediation efficacy in the form of a floating wetland for a maximum period of 10 days. The results were promising with a reduction of 34.36 ± 1.19 % of nitrates, 83.39 ± 0.66 % of phosphates and 97.79 ± 0.79% in bacterial load (cfu/mL). The consortia thus reared was found to have 79 ± 0.2 % protein, 19.8 ± 0.34 % lipids and 9 ± 0.42 % carbohydrates making it a prospective feed supplement. This pilot scale set up thus seems to be a promising solution for onsite remediation of agricultural run-offs that are rich in
nitrates and phosphates.