Iranian Journal of Pharmaceutical Research : IJPR, 2014
Elevated expression of the drug efflux transporter ABCG2 seems to correlate with multidrug resist... more Elevated expression of the drug efflux transporter ABCG2 seems to correlate with multidrug resistance of cancer cells. Specific COX-2 inhibitor celecoxib has been shown to enhance the sensitivity of cancer cells to anticancer drugs. To clarify whether ABCG2 inhibition is involved in the sensitizing effect of celecoxib, we investigated whether the expression of ABCG2 in breast cancer cell lines, could be modulated by celecoxib. The expression of the multidrug resistant gene (ABCG2) at mRNA and protein level was detected by real-time quantitative reverse transcription-polymerase chain reaction and flow cytometry analysis, respectively. Among three human breast cancer cell lines ABCG2 and COX-2 were highly expressed in MCF7-MX and MDA-MB-231 cells, respectively. The COX-2 inhibitor celecoxib up-regulated the expression of ABCG2 mRNA in MCF-7 and MCF7-MX cells, which was accompanied by increased ABCG2 protein expression. While celecoxib was able to block the 12-O-tetradecanoylphorbol-13...
The antigenotoxic effect of persicasulfide A (PSA) from Ferula persica on DNA damage induced by h... more The antigenotoxic effect of persicasulfide A (PSA) from Ferula persica on DNA damage induced by hydrogen peroxide (H2O2) was evaluated using single cell gel electrophoresis (SCGE). PSA was extracted from F. persica, characterized by NMR and its antioxidant/antigenotoxic effects were investigated. The antigenotoxic effect of solutions containing either PSA (1, 10, 50, 100, 200, 300, 400 and 500 microM) or ascorbic acid (250, 500, 750 and 1000 microM) alone, or in the presence of H2O2 (25, 50, 100 and 200 microM) were tested on lymphocytes derived from the blood of healthy male Wistar rats (250-300 g) by using the comet assay. The degree of damage to DNA after exposure to different solutions was calculated based on the amount of DNA present in the tail compared to the total amounts of lymphocyte DNA. PSA did not show genotoxicity and caused a 50% reduction in DNA damage induced by H2O (EC50:476.47+/-67.46 microM). Compared to the EC50 for ascorbic acid (1399.23+/-205.21 microM), it was deduced that PSA was more effective than ascorbic acid in the prevention of oxidative damage to DNA.
In this study, the antigenotoxic effects of diversin, a prenylated coumarin obtained from Ferula ... more In this study, the antigenotoxic effects of diversin, a prenylated coumarin obtained from Ferula diversivittata roots were evaluated using comet assay. Isolated lymphocytes from healthy volunteers' blood samples were incubated with diversin (10, 25, 50, 100, 200 and 400 µM) alone, or in the presence of H2O2 (25 µM). DNA break was measured based on the %tail DNA and compared with different concentrations of curcumin (10, 25 and 50 µM) as the positive control. It was shown that all concentrations of diversin significantly reduce DNA damage caused by H2O2.
The allele frequency of the multidrug resistance 1 (MDR1) gene C3435T polymorphism differs with d... more The allele frequency of the multidrug resistance 1 (MDR1) gene C3435T polymorphism differs with different ethnic populations, such as Asian, African, and Caucasian. This polymorphism has been reported to be associated with the increase of gastric cancer. The objective of this study was to find out the association of gastric cancer incidence with MDR1 gene polymorphism in an ethnic Iranian population. In this study, 48 gastric cancer patients were diagnosed. Genomic DNA was extracted by a salting-out method. The MDR1 polymorphism was studied by a polymerase chain reaction (PCR)-restriction fragment length polymorphism method, using a standard method. The polymorphic homozygote (T/T) genotype showed significantly an association with the incidence of gastric cancer compared with controls (P < 0.05). This study suggests that C3435T polymorphism of the MDR1 gene may be associated with gastric cancer in an ethnic Iranian population.
... damage. Javad Behravan, Fatemeh Mosaffa, Gholamreza Karimi, Mehrdad Iranshahi, and Samira Seh... more ... damage. Javad Behravan, Fatemeh Mosaffa, Gholamreza Karimi, Mehrdad Iranshahi, and Samira SehatBakhsh. Biotechnology Research Center, Mashhad University of Medical Sciences, Vakil abad Boulevard, Mashhad, Iran. ...
The protective properties of a prenylated coumarin, umbelliprenin (UMB), on the human lymphocytes... more The protective properties of a prenylated coumarin, umbelliprenin (UMB), on the human lymphocytes DNA lesions were tested. Lymphocytes were isolated from blood samples taken from healthy volunteers. DNA breaks and resistance to H(2)O(2)-induced damage were measured using a single-cell microgel electrophoresis technique under alkaline conditions (comet assay). Human lymphocytes were incubated in UMB (10, 25, 50, 100, 200, and 400 microM) alone or a combination of different concentrations of UMB (10, 25, 50, 100, 200, and 400 microM) and 25 microM H(2)O(2). Untreated cells, ascorbic acid (AA; 25, 50, 100, 200, and 400 microM) and H(2)O(2) (25 microM) were considered as negative control, positive control, and the standard antioxidant agent for our study, respectively. Single cells were analyzed with "TriTek Cometscore version 1.5" software. The DNA damage was expressed as percent tail DNA. UMB exhibited a concentration-dependent increase in protection activity against DNA damage induced by 25 microM H(2)O(2) (from 67.28% to 39.17%). The antigenotoxic activity of AA, in the range 0-50 microM, was greater than that of UMB. However, no significant difference (p > 0.05) in the protective activity was found between UMB and AA at concentrations of approximately higher than 50 microM.
Iranian Journal of Pharmaceutical Research : IJPR, 2014
Elevated expression of the drug efflux transporter ABCG2 seems to correlate with multidrug resist... more Elevated expression of the drug efflux transporter ABCG2 seems to correlate with multidrug resistance of cancer cells. Specific COX-2 inhibitor celecoxib has been shown to enhance the sensitivity of cancer cells to anticancer drugs. To clarify whether ABCG2 inhibition is involved in the sensitizing effect of celecoxib, we investigated whether the expression of ABCG2 in breast cancer cell lines, could be modulated by celecoxib. The expression of the multidrug resistant gene (ABCG2) at mRNA and protein level was detected by real-time quantitative reverse transcription-polymerase chain reaction and flow cytometry analysis, respectively. Among three human breast cancer cell lines ABCG2 and COX-2 were highly expressed in MCF7-MX and MDA-MB-231 cells, respectively. The COX-2 inhibitor celecoxib up-regulated the expression of ABCG2 mRNA in MCF-7 and MCF7-MX cells, which was accompanied by increased ABCG2 protein expression. While celecoxib was able to block the 12-O-tetradecanoylphorbol-13...
The antigenotoxic effect of persicasulfide A (PSA) from Ferula persica on DNA damage induced by h... more The antigenotoxic effect of persicasulfide A (PSA) from Ferula persica on DNA damage induced by hydrogen peroxide (H2O2) was evaluated using single cell gel electrophoresis (SCGE). PSA was extracted from F. persica, characterized by NMR and its antioxidant/antigenotoxic effects were investigated. The antigenotoxic effect of solutions containing either PSA (1, 10, 50, 100, 200, 300, 400 and 500 microM) or ascorbic acid (250, 500, 750 and 1000 microM) alone, or in the presence of H2O2 (25, 50, 100 and 200 microM) were tested on lymphocytes derived from the blood of healthy male Wistar rats (250-300 g) by using the comet assay. The degree of damage to DNA after exposure to different solutions was calculated based on the amount of DNA present in the tail compared to the total amounts of lymphocyte DNA. PSA did not show genotoxicity and caused a 50% reduction in DNA damage induced by H2O (EC50:476.47+/-67.46 microM). Compared to the EC50 for ascorbic acid (1399.23+/-205.21 microM), it was deduced that PSA was more effective than ascorbic acid in the prevention of oxidative damage to DNA.
In this study, the antigenotoxic effects of diversin, a prenylated coumarin obtained from Ferula ... more In this study, the antigenotoxic effects of diversin, a prenylated coumarin obtained from Ferula diversivittata roots were evaluated using comet assay. Isolated lymphocytes from healthy volunteers' blood samples were incubated with diversin (10, 25, 50, 100, 200 and 400 µM) alone, or in the presence of H2O2 (25 µM). DNA break was measured based on the %tail DNA and compared with different concentrations of curcumin (10, 25 and 50 µM) as the positive control. It was shown that all concentrations of diversin significantly reduce DNA damage caused by H2O2.
The allele frequency of the multidrug resistance 1 (MDR1) gene C3435T polymorphism differs with d... more The allele frequency of the multidrug resistance 1 (MDR1) gene C3435T polymorphism differs with different ethnic populations, such as Asian, African, and Caucasian. This polymorphism has been reported to be associated with the increase of gastric cancer. The objective of this study was to find out the association of gastric cancer incidence with MDR1 gene polymorphism in an ethnic Iranian population. In this study, 48 gastric cancer patients were diagnosed. Genomic DNA was extracted by a salting-out method. The MDR1 polymorphism was studied by a polymerase chain reaction (PCR)-restriction fragment length polymorphism method, using a standard method. The polymorphic homozygote (T/T) genotype showed significantly an association with the incidence of gastric cancer compared with controls (P < 0.05). This study suggests that C3435T polymorphism of the MDR1 gene may be associated with gastric cancer in an ethnic Iranian population.
... damage. Javad Behravan, Fatemeh Mosaffa, Gholamreza Karimi, Mehrdad Iranshahi, and Samira Seh... more ... damage. Javad Behravan, Fatemeh Mosaffa, Gholamreza Karimi, Mehrdad Iranshahi, and Samira SehatBakhsh. Biotechnology Research Center, Mashhad University of Medical Sciences, Vakil abad Boulevard, Mashhad, Iran. ...
The protective properties of a prenylated coumarin, umbelliprenin (UMB), on the human lymphocytes... more The protective properties of a prenylated coumarin, umbelliprenin (UMB), on the human lymphocytes DNA lesions were tested. Lymphocytes were isolated from blood samples taken from healthy volunteers. DNA breaks and resistance to H(2)O(2)-induced damage were measured using a single-cell microgel electrophoresis technique under alkaline conditions (comet assay). Human lymphocytes were incubated in UMB (10, 25, 50, 100, 200, and 400 microM) alone or a combination of different concentrations of UMB (10, 25, 50, 100, 200, and 400 microM) and 25 microM H(2)O(2). Untreated cells, ascorbic acid (AA; 25, 50, 100, 200, and 400 microM) and H(2)O(2) (25 microM) were considered as negative control, positive control, and the standard antioxidant agent for our study, respectively. Single cells were analyzed with "TriTek Cometscore version 1.5" software. The DNA damage was expressed as percent tail DNA. UMB exhibited a concentration-dependent increase in protection activity against DNA damage induced by 25 microM H(2)O(2) (from 67.28% to 39.17%). The antigenotoxic activity of AA, in the range 0-50 microM, was greater than that of UMB. However, no significant difference (p > 0.05) in the protective activity was found between UMB and AA at concentrations of approximately higher than 50 microM.
Uploads
Papers by F Mosaffa