A series of poly(vinyl alcohol) (PVA)/ Poly (vinyl pyrrolidone) (PVP) blend films with different ... more A series of poly(vinyl alcohol) (PVA)/ Poly (vinyl pyrrolidone) (PVP) blend films with different weight ratio of PVA to PVP were prepared by solution-mixing method The morphological structures and characterized of the films were observed via scanning electronic microscopy (SEM), Differential scanning calorimetry (DSC) and Fourier transform infrared (FTIR). DSC measurements showed that there was only one Tg for each blend and the values of Tg varied with the PVA content. This implies that the PVP/PVA blend is at least partially compatible. The formation of the hydrogen bonding between the PVP and PVA was identified by the FTIR spectra.
Iranian Journal of Pharmaceutical Research : IJPR, 2017
This study presents the first ever data of extracting chitin from the Chiton shell, which was the... more This study presents the first ever data of extracting chitin from the Chiton shell, which was then converted to the soluble chitosan by soaking in the 45% NaOH solution. The obtained chitin and chitosan were characterized by the seven different methods. Antioxidant activity of the extracted chitosan was also evaluated using the two methods. The shell content was divided into calcium carbonate (90.5 %), protein (5.2%), and chitin (4.3 %). Due to the results of element analysis and 1H NMR, the final degree of deacetylation of chitosan was 90%. Surprisingly, a significant amount of Fe was accidentally found in the shell after demineralization, and removed from the solution through the filtering. Nonetheless, remained Fe in the extracted chitin and chitosan was 20 times higher than those previously reported from the shell of shrimps and crabs. Presence of this amount of Fe could describe why the produced chitosan was darker compared to the commercial chitosan. Antioxidant activity tests...
This investigation determined the anticancer properties of zerumbone (ZER) on the human T-cell (J... more This investigation determined the anticancer properties of zerumbone (ZER) on the human T-cell (Jurkat) line using the MTT assay, microscopic evaluations, flow cytometric analyses, and caspase activity estimations. The results showed that ZER is selectively cytotoxic to Jurkat cells in a dose and time-dependent manner with IC50 of 11.9 ± 0.2, 8.6 ± 0.5 and 5.4 ± 0.4 μg/mL at 24, 48 and 72 hours of treatment, respectively. ZER did not produce an adverse effect on normal human peripheral blood mononuclear cells (PBMC). ZER is not as cytotoxic as doxorubicin, which imposed an inhibitory effect on Jurkat cells with IC50 of 2.1 ± 0.2, 1.8 ± 0.15, 1.5 ± 0.07 μg/mL after 24, 48 and 72 hours treatment, respectively. ZER significantly (P<0.05 ) arrested Jurkat cells at the G2/M phase of the cell cycle. The antiproliferative effect of ZER on Jurkat cells was through the apoptotic intrinsic pathway via the activation of caspase-3 and -9. The results showed that ZER can be further developed ...
ABSTRACT Persian Shallot (Allium ascalonicum Linn.) has been proven to possess some antimicrobial... more ABSTRACT Persian Shallot (Allium ascalonicum Linn.) has been proven to possess some antimicrobial properties against some of bacteria, viruses and fungi. Indeed the aim of this study was to investigate the antifungal properties of several Persian Shallot extracts against important Candida species. In the first stage the preliminary screening of antifungal activity was determined by disc diffusion test. The effective extracts (hexane, ethyl acetate, methanol, and water) were proceeded to obtain minimum inhibitory concentration using broth microdilution test and time-kill study was also performed at time intervals. Results showed that the Shallot-hexane extract was more effective in terms of Candida species growth. Subsequently, the effects of the extracts on C. albicans biofilm were determined using XTT assay. Finally the expression level of a crucial gene involved in biofilm formation in C. albicans was analysed by semi-quantitative RT-PCR. The MIC was ranged from 5-600 μg/mL for different species of Candida. Moreover in time-kill study, no colonies were observed at 1× MIC after 24 h of incubation period. On the other hand, XTT assay showed the significant reduction of biofilm in Candidatreated by Shallotat level p&lt;0.05. Eventually the expression level of HWP1 was down regulated through the semi-quantitative RT-PCR (p&lt; 0.05). The present study indicates that HWP1 could be one of the possible targets of Shallot to inhibit biofilm in C. albicans.
A comestible composition with anti-cancer property comprises alcohol soluble extracts from plant ... more A comestible composition with anti-cancer property comprises alcohol soluble extracts from plant part of Piper species, palm species, tropical seaweeds, and/or Solanum species
Zerumbone- (ZER-) loaded nanostructure lipid carrier (NLC) (ZER-NLC) prepared for its antileukemi... more Zerumbone- (ZER-) loaded nanostructure lipid carrier (NLC) (ZER-NLC) prepared for its antileukemia effectin vitrowas evaluated for its toxicological effects by observing changes in the liver, kidney, spleen, lung, heart, and brain tissues, serum biochemical parameters, total haemogram, and bone marrow stem cells. The acute toxicity study for ZER-NLC was conducted by orally treating BALB/c mice with a single dose with either water, olive oil, ZER, NLC, or ZER-NLC for 14 days. The animals were observed for clinical and behavioral abnormalities, toxicological symptoms, feed consumption, and gross appearance. The liver, kidney, heart, lung, spleen, and brain tissues were assessed histologically. Total haemogram was counted by hemocytometry and microhematocrit reader. Bone marrow examination in terms of cellular morphology was done by Wright staining with bone marrow smear. Furthermore, serum biochemical parameters were determined spectrophotometrically. Grossly all treated mice, their i...
A series of poly(vinyl alcohol) (PVA)/ Poly (vinyl pyrrolidone) (PVP) blend films with different ... more A series of poly(vinyl alcohol) (PVA)/ Poly (vinyl pyrrolidone) (PVP) blend films with different weight ratio of PVA to PVP were prepared by solution-mixing method The morphological structures and characterized of the films were observed via scanning electronic microscopy (SEM), Differential scanning calorimetry (DSC) and Fourier transform infrared (FTIR). DSC measurements showed that there was only one Tg for each blend and the values of Tg varied with the PVA content. This implies that the PVP/PVA blend is at least partially compatible. The formation of the hydrogen bonding between the PVP and PVA was identified by the FTIR spectra.
Iranian Journal of Pharmaceutical Research : IJPR, 2017
This study presents the first ever data of extracting chitin from the Chiton shell, which was the... more This study presents the first ever data of extracting chitin from the Chiton shell, which was then converted to the soluble chitosan by soaking in the 45% NaOH solution. The obtained chitin and chitosan were characterized by the seven different methods. Antioxidant activity of the extracted chitosan was also evaluated using the two methods. The shell content was divided into calcium carbonate (90.5 %), protein (5.2%), and chitin (4.3 %). Due to the results of element analysis and 1H NMR, the final degree of deacetylation of chitosan was 90%. Surprisingly, a significant amount of Fe was accidentally found in the shell after demineralization, and removed from the solution through the filtering. Nonetheless, remained Fe in the extracted chitin and chitosan was 20 times higher than those previously reported from the shell of shrimps and crabs. Presence of this amount of Fe could describe why the produced chitosan was darker compared to the commercial chitosan. Antioxidant activity tests...
This investigation determined the anticancer properties of zerumbone (ZER) on the human T-cell (J... more This investigation determined the anticancer properties of zerumbone (ZER) on the human T-cell (Jurkat) line using the MTT assay, microscopic evaluations, flow cytometric analyses, and caspase activity estimations. The results showed that ZER is selectively cytotoxic to Jurkat cells in a dose and time-dependent manner with IC50 of 11.9 ± 0.2, 8.6 ± 0.5 and 5.4 ± 0.4 μg/mL at 24, 48 and 72 hours of treatment, respectively. ZER did not produce an adverse effect on normal human peripheral blood mononuclear cells (PBMC). ZER is not as cytotoxic as doxorubicin, which imposed an inhibitory effect on Jurkat cells with IC50 of 2.1 ± 0.2, 1.8 ± 0.15, 1.5 ± 0.07 μg/mL after 24, 48 and 72 hours treatment, respectively. ZER significantly (P<0.05 ) arrested Jurkat cells at the G2/M phase of the cell cycle. The antiproliferative effect of ZER on Jurkat cells was through the apoptotic intrinsic pathway via the activation of caspase-3 and -9. The results showed that ZER can be further developed ...
ABSTRACT Persian Shallot (Allium ascalonicum Linn.) has been proven to possess some antimicrobial... more ABSTRACT Persian Shallot (Allium ascalonicum Linn.) has been proven to possess some antimicrobial properties against some of bacteria, viruses and fungi. Indeed the aim of this study was to investigate the antifungal properties of several Persian Shallot extracts against important Candida species. In the first stage the preliminary screening of antifungal activity was determined by disc diffusion test. The effective extracts (hexane, ethyl acetate, methanol, and water) were proceeded to obtain minimum inhibitory concentration using broth microdilution test and time-kill study was also performed at time intervals. Results showed that the Shallot-hexane extract was more effective in terms of Candida species growth. Subsequently, the effects of the extracts on C. albicans biofilm were determined using XTT assay. Finally the expression level of a crucial gene involved in biofilm formation in C. albicans was analysed by semi-quantitative RT-PCR. The MIC was ranged from 5-600 μg/mL for different species of Candida. Moreover in time-kill study, no colonies were observed at 1× MIC after 24 h of incubation period. On the other hand, XTT assay showed the significant reduction of biofilm in Candidatreated by Shallotat level p&lt;0.05. Eventually the expression level of HWP1 was down regulated through the semi-quantitative RT-PCR (p&lt; 0.05). The present study indicates that HWP1 could be one of the possible targets of Shallot to inhibit biofilm in C. albicans.
A comestible composition with anti-cancer property comprises alcohol soluble extracts from plant ... more A comestible composition with anti-cancer property comprises alcohol soluble extracts from plant part of Piper species, palm species, tropical seaweeds, and/or Solanum species
Zerumbone- (ZER-) loaded nanostructure lipid carrier (NLC) (ZER-NLC) prepared for its antileukemi... more Zerumbone- (ZER-) loaded nanostructure lipid carrier (NLC) (ZER-NLC) prepared for its antileukemia effectin vitrowas evaluated for its toxicological effects by observing changes in the liver, kidney, spleen, lung, heart, and brain tissues, serum biochemical parameters, total haemogram, and bone marrow stem cells. The acute toxicity study for ZER-NLC was conducted by orally treating BALB/c mice with a single dose with either water, olive oil, ZER, NLC, or ZER-NLC for 14 days. The animals were observed for clinical and behavioral abnormalities, toxicological symptoms, feed consumption, and gross appearance. The liver, kidney, heart, lung, spleen, and brain tissues were assessed histologically. Total haemogram was counted by hemocytometry and microhematocrit reader. Bone marrow examination in terms of cellular morphology was done by Wright staining with bone marrow smear. Furthermore, serum biochemical parameters were determined spectrophotometrically. Grossly all treated mice, their i...
Uploads
Papers by Farideh Namvar