We have determined the sequence of 250 bases 5' of the transcriptional start site of the apol... more We have determined the sequence of 250 bases 5' of the transcriptional start site of the apolipoprotein AI gene in a human individual with high serum concentrations of apo AI. One of the alleles contained a G to A substitution at position -75, between the CACAT sequence and the TAAATA box, creating a tandem repeat, CAGGGC-CA*GGGC. The substitution destroys an MspI cutting site, and the polymerase chain reaction and MspI digestion were used to identify the presence of the A or G base. The frequency of the A substitution in 96 healthy men from Bristol was 0.11 and this was increased to 0.25 in men with serum apo AI concentrations greater than 180 mg/dl. Men with the A allele had significantly higher serum concentrations of apo AI, high density lipoprotein (HDL) cholesterol and HDL2 than those with the G allele. In this sample, variation associated with the G to A substitution accounted for 6% and 4.6% of the total variance in apo AI and HDL cholesterol concentrations, respectively...
Die Delphi-Technik in den Sozialwissenschaften, 2000
There are two objectives in the context of foresight in South Africa discussed in this contributi... more There are two objectives in the context of foresight in South Africa discussed in this contribution. Firstly, we want to provide the reader with a broad insight into the processes we are involved with in the country and secondly to share some of the results from our survey of twelve sectors. The change in government and the reality of our democratization process has bought on new challenges and imperatives. The previous Government had three major priorities within the context of Science and Technology: 1. Military dominance in the sub-continent 2. Energy self-sufficiency and 3. National food security
Health research observatories are globally recognised proactive institutions that provide appropr... more Health research observatories are globally recognised proactive institutions that provide appropriate evidence-based information to guide policy-making decisions in a country, thereby improving health care. The World Health Organization has recommended the development of national and regional research and development observatories to ensure relevant, timeous and credible information on current and emerging health research priorities and impact on health responses and interventions, including allocation of resources and their scope and location. This chapter describes the vision, mandate, purpose, scope and benefits of a proposed health research observatory, as well as the key challenges, in order to stimulate awareness and generate local interest among and participation by the health research community and relevant stakeholders in South Africa. The National Health Research Committee (NHRC) has undertaken to establish the National Health Research Observatory as a comprehensive inform...
Simvastatin, an inhibitor of HMG CoA reductase, lowers the plasma total cholesterol and LDL-chole... more Simvastatin, an inhibitor of HMG CoA reductase, lowers the plasma total cholesterol and LDL-cholesterol concentration in familial hypercholesterolemic patients. The efficacy of the drug shows considerable inter-individual variation, however. In this study we have assessed the influence of certain LDL-receptor gene mutations on this variation. A group of 20 male and female heterozygotic familial hypercholesterolemic patients, all Afrikaners and each bearing one of two different LDL receptor gene mutations, FH Afrikaner-1 (FH1) and FH Afrikaner-2 (FH2), was treated with simvastatin (40 mg once daily) for 18 months. The average reduction in total plasma cholesterol was 35.3% in the case of the FH2 men but only 23.2% in that of the FH1 men (P = 0.005); the reduction in LDL cholesterol concentrations was also greater in the FH2 group (39% as opposed to 27.1%, P = 0.02). The better response of the FH2 group was also evident when men and women were considered together. Female FH1 patients responded better to simvastatin treatment, however, than did males with the same gene defect. Mutations at the LDL-receptor locus may thus play a significant role in the variable efficacy of the drug. The particular mutations in the males of this group may have contributed up to 35% of the variance in total cholesterol response and 29% of the variance in LDL-cholesterol response to simvastatin treatment.
We have determined the sequence of 250 bases 5' of the transcriptional start site of the apol... more We have determined the sequence of 250 bases 5' of the transcriptional start site of the apolipoprotein AI gene in a human individual with high serum concentrations of apo AI. One of the alleles contained a G to A substitution at position -75, between the CACAT sequence and the TAAATA box, creating a tandem repeat, CAGGGC-CA*GGGC. The substitution destroys an MspI cutting site, and the polymerase chain reaction and MspI digestion were used to identify the presence of the A or G base. The frequency of the A substitution in 96 healthy men from Bristol was 0.11 and this was increased to 0.25 in men with serum apo AI concentrations greater than 180 mg/dl. Men with the A allele had significantly higher serum concentrations of apo AI, high density lipoprotein (HDL) cholesterol and HDL2 than those with the G allele. In this sample, variation associated with the G to A substitution accounted for 6% and 4.6% of the total variance in apo AI and HDL cholesterol concentrations, respectively...
Die Delphi-Technik in den Sozialwissenschaften, 2000
There are two objectives in the context of foresight in South Africa discussed in this contributi... more There are two objectives in the context of foresight in South Africa discussed in this contribution. Firstly, we want to provide the reader with a broad insight into the processes we are involved with in the country and secondly to share some of the results from our survey of twelve sectors. The change in government and the reality of our democratization process has bought on new challenges and imperatives. The previous Government had three major priorities within the context of Science and Technology: 1. Military dominance in the sub-continent 2. Energy self-sufficiency and 3. National food security
Health research observatories are globally recognised proactive institutions that provide appropr... more Health research observatories are globally recognised proactive institutions that provide appropriate evidence-based information to guide policy-making decisions in a country, thereby improving health care. The World Health Organization has recommended the development of national and regional research and development observatories to ensure relevant, timeous and credible information on current and emerging health research priorities and impact on health responses and interventions, including allocation of resources and their scope and location. This chapter describes the vision, mandate, purpose, scope and benefits of a proposed health research observatory, as well as the key challenges, in order to stimulate awareness and generate local interest among and participation by the health research community and relevant stakeholders in South Africa. The National Health Research Committee (NHRC) has undertaken to establish the National Health Research Observatory as a comprehensive inform...
Simvastatin, an inhibitor of HMG CoA reductase, lowers the plasma total cholesterol and LDL-chole... more Simvastatin, an inhibitor of HMG CoA reductase, lowers the plasma total cholesterol and LDL-cholesterol concentration in familial hypercholesterolemic patients. The efficacy of the drug shows considerable inter-individual variation, however. In this study we have assessed the influence of certain LDL-receptor gene mutations on this variation. A group of 20 male and female heterozygotic familial hypercholesterolemic patients, all Afrikaners and each bearing one of two different LDL receptor gene mutations, FH Afrikaner-1 (FH1) and FH Afrikaner-2 (FH2), was treated with simvastatin (40 mg once daily) for 18 months. The average reduction in total plasma cholesterol was 35.3% in the case of the FH2 men but only 23.2% in that of the FH1 men (P = 0.005); the reduction in LDL cholesterol concentrations was also greater in the FH2 group (39% as opposed to 27.1%, P = 0.02). The better response of the FH2 group was also evident when men and women were considered together. Female FH1 patients responded better to simvastatin treatment, however, than did males with the same gene defect. Mutations at the LDL-receptor locus may thus play a significant role in the variable efficacy of the drug. The particular mutations in the males of this group may have contributed up to 35% of the variance in total cholesterol response and 29% of the variance in LDL-cholesterol response to simvastatin treatment.
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Papers by Mohammed Jeenah