Pakistan journal of biological sciences: PJBS, 2007
1. Pak J Biol Sci. 2007 Nov 15;10(22):3973-82. Study the effect of hexavalent chromium on some bi... more 1. Pak J Biol Sci. 2007 Nov 15;10(22):3973-82. Study the effect of hexavalent chromium on some biochemical, citotoxicological and histopathological aspects of the Orechromis spp. fish. Abbas HH, Ali FK. Department of Hydrobiology ...
There is rising concern for the interaction of environmental contaminants with brain transcriptom... more There is rising concern for the interaction of environmental contaminants with brain transcriptome and the potential effect on reproductive processes. The present study sought to determine selenoprotein glutathione peroxidase 4 (gpx4) transcriptional activity in the brain and testis of the soil biosentinel, Podarcis sicula, through the main phases of the reproductive cycle and whether pharmaceuticals exert an endocrine disruption. Based on gpx4 cloned amminoacids sequence (GenBank AEX09236.1.), we used a bioinformatic approach to assess the structural role. Specifically, we detected seasonally the reactive oxygen species (ROS) level using electron spin resonance spectroscopy and gpx4 transcriptional activity using quantitative real‐time polymerase chain reaction. In addition, the impact of pharmaceuticals was assessed after 21‐days of treatment with ICI 182,780 and human chorionic gonadotropin administration in mating and winter stasis, respectively. Bioinformatic data shows the gpx4 proteic activity and a phylogenetic profile. ROS contents in lizard brain are significantly less than in testis and display higher levels after treatments. Brain gpx4 expression gives statistically significant seasonal differences, opposite trends in testis and altered expression in both tissues, with evidence of testis morphological and DNA disruption. Taken together, these results provide direct evidence that gpx4 in P. sicula plays a seasonal regulatory role and may be a reliable biomarker for reproductive health toxicity screening.
Dioxins have global concerns because of the bioaccumulation tendency and persistency in the envir... more Dioxins have global concerns because of the bioaccumulation tendency and persistency in the environment. Water, seabream Pagrus auratus and seabass Dicentrarchus labrax samples were collected from Abu Qir, Alexandria to evaluate the concentration of dioxin. Fourier Transform Infrared Spectrometer (FTIR) and molecular modeling was applied for elucidating the molecular structure of fish samples. Furthermore, HPLC with UV detection was used to determine the concentration of dioxins (2,8-dichloro dibenzo-p-dioxin). RT-PCR assay was conducted to verify the expression of some immune genes in the fish species as a result of water pollution. The average detected concentrations varied from 0.2 to 1.3μg/l. Gene expression revealed that MHC class 1 and C3 were highly upregulated in liver and muscle of seabass and seabream while T2BP was highly regulated in seabass liver and seabream muscle and seabass muscle for transferrin, FTIR and molecular modeling indicate that dioxin finds its way to fis...
Reactive oxygen species (ROS) in biological tissues of elected biosentinels represent an optimal ... more Reactive oxygen species (ROS) in biological tissues of elected biosentinels represent an optimal biomarker for eco-monitoring of polluted areas. Electron spin resonance (ESR) is the most definitive method for detecting, quantifying and possibly identifying radicals in complex systems. A non-invasive method for monitoring polluted areas by the quantitative determination of ROS in frog skin biopsy is presented. We assessed by ESR spectroscopy the ROS level in adult male of Pelophylax bergeri, specie not a risk of extinction, collected from the polluted Sarno River (SA, Italy) basin. The spin-trap ESR method was validated by immunohistochemical analysis of the well-assessed pollution biomarkers cytochrome P450 aromatase 1A (CYP1A) and glutathione S-transferase (GST), and by determining the poly(ADPribose) polymerase (PARP) and GST enzymatic activity. ROS concentration in skin samples from frogs collected in the polluted area is significantly higher than that determined for the unpollut...
Environmental science and pollution research international, Jan 28, 2017
Model of the our research was the adult male amphibian anura, Pelophylax bergeri, poikilotherm sp... more Model of the our research was the adult male amphibian anura, Pelophylax bergeri, poikilotherm species not considered threatened by the IUCN, sampled in representative sites at different degree. In the first phase, a biochemical characterization of the ADP-ribosylation on the skin of barcoded amphibian anura collected from Matese Lake (clean reference site in CE, Italy) was carried out. Two PARP isoforms were evidence: the first of 66 kDa is localized into nucleus and activated by DNA damage; the second of 150 kDa is in cytoplasm, as demonstrated by biochemical and immunohistochemical analysis. Subsequently, the PARP activity, the quantitative expression of androgen receptor gene, and the levels of arsenic and chromium in skin and testis of frog and soil, water, and sediment collected from sites at different degrees of pollution were measured. A significant variation of PARP activity and androgen receptor expression levels was detected in both tissues of barcoded frogs from Sarno an...
We have developed an original in vitro bioassay using teleost scale, that has osteoclasts, osteob... more We have developed an original in vitro bioassay using teleost scale, that has osteoclasts, osteoblasts, and bone matrix as each marker: alkaline phosphatase (ALP) for osteoblasts and tartrate-resistant acid phosphatase (TRAP) for osteoclasts. Using this scale in vitro bioassay, we examined the effects of seawater polluted with highly concentrated polycyclic aromatic hydrocarbons (PAHs) and nitro-polycyclic aromatic hydrocarbons (NPAHs) on osteoblastic and osteoclastic activities in the present study. Polluted seawater was collected from two sites (the Alexandria site on the Mediterranean Sea and the Suez Canal site on the Red Sea). Total levels of PAHs in the seawater from the Alexandria and Suez Canal sites were 1364.59 and 992.56 ng/l, respectively. We were able to detect NPAHs in both seawater samples. Total levels of NPAHs were detected in the seawater of the Alexandria site (12.749 ng/l) and the Suez Canal site (3.914 ng/l). Each sample of polluted seawater was added to culture...
Interaction between oil pollution status and cellular antioxidant defense by Glutathione S-Transf... more Interaction between oil pollution status and cellular antioxidant defense by Glutathione S-Transferase (GST) was investigated using marine fish. The results in grouper, Solea vulgaris revealed that grouper liver collected from the Suez oil production port, Floating port, resulted in significant decrease in the GST activity respect samples from Atakah fishing port. The liver GST parameter in S. vulgaris could be extended as a biomarker of oil Suez Canal pollution.
Aeromonads have been recognized as potential food borne pathogens for more than 20 years. Aeromon... more Aeromonads have been recognized as potential food borne pathogens for more than 20 years. Aeromonas species is ubiquitous in aquatic environments / components including fresh water, fish and shellfish. Clinical infections of Aeromonads usually result in heavy losses in both marine and freshwater aquaculture. The main goal of our research was to investigate the occurrence of potentially pathogenic Aeromonas species in the River Nile, Egypt during the year 2009/2010. Six hundred and twenty six random isolates of typical colonies of Aeromonads were isolated from water/ fish and then further confirmed by PCR. Moreover, we have demonstrated the DNA damage in fish organs because of exposure to polluted water. Five hundred and sixty one isolates were confirmed to be Aeromonas species. Only two hundred and seventy six isolates were confirmed to be A. hydrophila when strains were analyzed for the presence of the aerolysin (aerA) and hemolysin (hylaA) genes by PCR amplification using specific...
Infectious, water-related diseases are a major cause of morbidity and mortality worldwide. A sign... more Infectious, water-related diseases are a major cause of morbidity and mortality worldwide. A significant proportion of this immense burden of diseases is caused by ‘classical’ water-related pollutants. In the Egyptian water streams there are a number of compounds that are toxic even at relatively low levels; these pollutants were identified as “priority pollutants.” Such pollutants have a particular concern for aquatic systems include: dichlorodiphenyl trichloroethane (DDT) and polycyclic aromatic hydrocarbons (PAHs) and their nitrated derivatives (NPAHs). Acute exposure to these substances produce adverse effects of aquatic biota and habitats, chronic exposure to low concentrations probably is a more significant issue for fish population structure and may result in multiple substances acting in “an additive, synergistic or antagonistic manner” that may render impacts relatively difficult to discern. As a result, these pollutants can act as endocrine disrupting chemicals (EDCs). The...
In the current investigation the cyanobacterium; Spirulina maxima exhibited antagonistic activity... more In the current investigation the cyanobacterium; Spirulina maxima exhibited antagonistic activity against Pencillium oxalicum. The results indicated that, the active substance produced maximally after 15 days of incubation in aerated culture at 30°C and pH 8 in B-G 11 medium. Dimethyl was the best solvent for extracting the active material. The antagonistic material was purified using thin layer chromatography. It was found that the purified antagonistic agent produced had a moderately toxic level and the LC was 1027 mg/l. The partially 50 purified agent of S. maxima showed a broad spectrum of antifungal activity with an average activity of 26% against five tested human and plant pathogenic fungi compared to the three tested commercial drugs. The most inhibited fungus was P. oxalicum (91%) followed by F. solani (65%) and R. solani (20%) compared to the tested antifungal drugs. The compound showed maximum absorption band at 250 nm. Infrared (IR) indicated presence of (NH , OH, NH) gr...
Polycyclic aromatic hydrocarbons (PAHs) in the Red sea water were determined at Suez and Ismailia... more Polycyclic aromatic hydrocarbons (PAHs) in the Red sea water were determined at Suez and Ismailia governorates, Egypt. The sites were selected to represent areas with different activities of Suez Gulf water. The concentrations of fifteen PAHs having two to six rings were determined by using HPLC with florescence detection. The total average concentrations of the fifteen PAHs over the Suez governorate sites (S1 and S2) were 1.45 and 0.34 μg/l, respectively. However those for Ismailia governorate sites (I1 and I2) were 1.06 and 0.24 μg/l, respectively. PAHs having four to six rings were the predominant compounds in particulate matters. The major sources of PAHs and their impacts on the health of certain types of fish such as Mullet and Sea-bass species were studied through the effect on the expression of stress protein related genes. In addition, expression of stress protein related genes (Cytochrome P450, CYP1A and metallothionein, MT-1A) and antioxidant enzymes (Glutathione-S-Transf...
International Journal of Scientific and Engineering Research
The Sarno River is affected by an extreme environmental degradation as a result of uncontrolled a... more The Sarno River is affected by an extreme environmental degradation as a result of uncontrolled agricultural activities and the outflow of industrial waste. Changes in the abundance indexes and biodiversity of frogs can be used as a measuring tool to determine water quality of the Sarno River. The aim of this study is to demonstrate the potential in using frog tissue as environmental indicators and to monitor changes in water quality of local streams in the development area of Sarno River. Thus, many samples are collected from sediment, water and frogs for metals analysis. To identify any differentiations of bioaccumulation of metals we have been analyzed skin and gonad of the more representative frog specie along the Sarno river, the barcoded Pelophylax bergeri. Results show that the degree of pollution in the Sarno river varies by zone, as well as by metal to metal, with contents that often are very elevated in frogs and higher in water than sediment. The concentrations of heavy m...
The aquatic environment makes up the major part of our environment and resources, therefore its s... more The aquatic environment makes up the major part of our environment and resources, therefore its safety is directly related to the safety our health. In this study, three tilapia species ( Oreochromis niloticus , Oreochromis aureus and Tilapia zilli ) and Clarias gariepinus were employed to estimate water pollution using micronucleus (MN) test. The test has been used successfully as a mutagenic assay. It is simple, reliable, sensitive, and it does not depend on any karyotypic characteristics. Fish were collected from locations that display differential environmental stresses. Two main experiments were carried out. In the first one, blood samples were collected, fixed for 24 h and then were stained with Giemsa. In the second experiment, fish were acclimated for a week. Fish were fed and each specimen had received an IP injection of cyclophosphamide (2.6, 10, 40, mg/kg b.wt). After 24 h, blood samples were collected and MN frequencies were counted and statistically tested. Results from...
Pakistan journal of biological sciences: PJBS, 2007
1. Pak J Biol Sci. 2007 Nov 15;10(22):3973-82. Study the effect of hexavalent chromium on some bi... more 1. Pak J Biol Sci. 2007 Nov 15;10(22):3973-82. Study the effect of hexavalent chromium on some biochemical, citotoxicological and histopathological aspects of the Orechromis spp. fish. Abbas HH, Ali FK. Department of Hydrobiology ...
There is rising concern for the interaction of environmental contaminants with brain transcriptom... more There is rising concern for the interaction of environmental contaminants with brain transcriptome and the potential effect on reproductive processes. The present study sought to determine selenoprotein glutathione peroxidase 4 (gpx4) transcriptional activity in the brain and testis of the soil biosentinel, Podarcis sicula, through the main phases of the reproductive cycle and whether pharmaceuticals exert an endocrine disruption. Based on gpx4 cloned amminoacids sequence (GenBank AEX09236.1.), we used a bioinformatic approach to assess the structural role. Specifically, we detected seasonally the reactive oxygen species (ROS) level using electron spin resonance spectroscopy and gpx4 transcriptional activity using quantitative real‐time polymerase chain reaction. In addition, the impact of pharmaceuticals was assessed after 21‐days of treatment with ICI 182,780 and human chorionic gonadotropin administration in mating and winter stasis, respectively. Bioinformatic data shows the gpx4 proteic activity and a phylogenetic profile. ROS contents in lizard brain are significantly less than in testis and display higher levels after treatments. Brain gpx4 expression gives statistically significant seasonal differences, opposite trends in testis and altered expression in both tissues, with evidence of testis morphological and DNA disruption. Taken together, these results provide direct evidence that gpx4 in P. sicula plays a seasonal regulatory role and may be a reliable biomarker for reproductive health toxicity screening.
Dioxins have global concerns because of the bioaccumulation tendency and persistency in the envir... more Dioxins have global concerns because of the bioaccumulation tendency and persistency in the environment. Water, seabream Pagrus auratus and seabass Dicentrarchus labrax samples were collected from Abu Qir, Alexandria to evaluate the concentration of dioxin. Fourier Transform Infrared Spectrometer (FTIR) and molecular modeling was applied for elucidating the molecular structure of fish samples. Furthermore, HPLC with UV detection was used to determine the concentration of dioxins (2,8-dichloro dibenzo-p-dioxin). RT-PCR assay was conducted to verify the expression of some immune genes in the fish species as a result of water pollution. The average detected concentrations varied from 0.2 to 1.3μg/l. Gene expression revealed that MHC class 1 and C3 were highly upregulated in liver and muscle of seabass and seabream while T2BP was highly regulated in seabass liver and seabream muscle and seabass muscle for transferrin, FTIR and molecular modeling indicate that dioxin finds its way to fis...
Reactive oxygen species (ROS) in biological tissues of elected biosentinels represent an optimal ... more Reactive oxygen species (ROS) in biological tissues of elected biosentinels represent an optimal biomarker for eco-monitoring of polluted areas. Electron spin resonance (ESR) is the most definitive method for detecting, quantifying and possibly identifying radicals in complex systems. A non-invasive method for monitoring polluted areas by the quantitative determination of ROS in frog skin biopsy is presented. We assessed by ESR spectroscopy the ROS level in adult male of Pelophylax bergeri, specie not a risk of extinction, collected from the polluted Sarno River (SA, Italy) basin. The spin-trap ESR method was validated by immunohistochemical analysis of the well-assessed pollution biomarkers cytochrome P450 aromatase 1A (CYP1A) and glutathione S-transferase (GST), and by determining the poly(ADPribose) polymerase (PARP) and GST enzymatic activity. ROS concentration in skin samples from frogs collected in the polluted area is significantly higher than that determined for the unpollut...
Environmental science and pollution research international, Jan 28, 2017
Model of the our research was the adult male amphibian anura, Pelophylax bergeri, poikilotherm sp... more Model of the our research was the adult male amphibian anura, Pelophylax bergeri, poikilotherm species not considered threatened by the IUCN, sampled in representative sites at different degree. In the first phase, a biochemical characterization of the ADP-ribosylation on the skin of barcoded amphibian anura collected from Matese Lake (clean reference site in CE, Italy) was carried out. Two PARP isoforms were evidence: the first of 66 kDa is localized into nucleus and activated by DNA damage; the second of 150 kDa is in cytoplasm, as demonstrated by biochemical and immunohistochemical analysis. Subsequently, the PARP activity, the quantitative expression of androgen receptor gene, and the levels of arsenic and chromium in skin and testis of frog and soil, water, and sediment collected from sites at different degrees of pollution were measured. A significant variation of PARP activity and androgen receptor expression levels was detected in both tissues of barcoded frogs from Sarno an...
We have developed an original in vitro bioassay using teleost scale, that has osteoclasts, osteob... more We have developed an original in vitro bioassay using teleost scale, that has osteoclasts, osteoblasts, and bone matrix as each marker: alkaline phosphatase (ALP) for osteoblasts and tartrate-resistant acid phosphatase (TRAP) for osteoclasts. Using this scale in vitro bioassay, we examined the effects of seawater polluted with highly concentrated polycyclic aromatic hydrocarbons (PAHs) and nitro-polycyclic aromatic hydrocarbons (NPAHs) on osteoblastic and osteoclastic activities in the present study. Polluted seawater was collected from two sites (the Alexandria site on the Mediterranean Sea and the Suez Canal site on the Red Sea). Total levels of PAHs in the seawater from the Alexandria and Suez Canal sites were 1364.59 and 992.56 ng/l, respectively. We were able to detect NPAHs in both seawater samples. Total levels of NPAHs were detected in the seawater of the Alexandria site (12.749 ng/l) and the Suez Canal site (3.914 ng/l). Each sample of polluted seawater was added to culture...
Interaction between oil pollution status and cellular antioxidant defense by Glutathione S-Transf... more Interaction between oil pollution status and cellular antioxidant defense by Glutathione S-Transferase (GST) was investigated using marine fish. The results in grouper, Solea vulgaris revealed that grouper liver collected from the Suez oil production port, Floating port, resulted in significant decrease in the GST activity respect samples from Atakah fishing port. The liver GST parameter in S. vulgaris could be extended as a biomarker of oil Suez Canal pollution.
Aeromonads have been recognized as potential food borne pathogens for more than 20 years. Aeromon... more Aeromonads have been recognized as potential food borne pathogens for more than 20 years. Aeromonas species is ubiquitous in aquatic environments / components including fresh water, fish and shellfish. Clinical infections of Aeromonads usually result in heavy losses in both marine and freshwater aquaculture. The main goal of our research was to investigate the occurrence of potentially pathogenic Aeromonas species in the River Nile, Egypt during the year 2009/2010. Six hundred and twenty six random isolates of typical colonies of Aeromonads were isolated from water/ fish and then further confirmed by PCR. Moreover, we have demonstrated the DNA damage in fish organs because of exposure to polluted water. Five hundred and sixty one isolates were confirmed to be Aeromonas species. Only two hundred and seventy six isolates were confirmed to be A. hydrophila when strains were analyzed for the presence of the aerolysin (aerA) and hemolysin (hylaA) genes by PCR amplification using specific...
Infectious, water-related diseases are a major cause of morbidity and mortality worldwide. A sign... more Infectious, water-related diseases are a major cause of morbidity and mortality worldwide. A significant proportion of this immense burden of diseases is caused by ‘classical’ water-related pollutants. In the Egyptian water streams there are a number of compounds that are toxic even at relatively low levels; these pollutants were identified as “priority pollutants.” Such pollutants have a particular concern for aquatic systems include: dichlorodiphenyl trichloroethane (DDT) and polycyclic aromatic hydrocarbons (PAHs) and their nitrated derivatives (NPAHs). Acute exposure to these substances produce adverse effects of aquatic biota and habitats, chronic exposure to low concentrations probably is a more significant issue for fish population structure and may result in multiple substances acting in “an additive, synergistic or antagonistic manner” that may render impacts relatively difficult to discern. As a result, these pollutants can act as endocrine disrupting chemicals (EDCs). The...
In the current investigation the cyanobacterium; Spirulina maxima exhibited antagonistic activity... more In the current investigation the cyanobacterium; Spirulina maxima exhibited antagonistic activity against Pencillium oxalicum. The results indicated that, the active substance produced maximally after 15 days of incubation in aerated culture at 30°C and pH 8 in B-G 11 medium. Dimethyl was the best solvent for extracting the active material. The antagonistic material was purified using thin layer chromatography. It was found that the purified antagonistic agent produced had a moderately toxic level and the LC was 1027 mg/l. The partially 50 purified agent of S. maxima showed a broad spectrum of antifungal activity with an average activity of 26% against five tested human and plant pathogenic fungi compared to the three tested commercial drugs. The most inhibited fungus was P. oxalicum (91%) followed by F. solani (65%) and R. solani (20%) compared to the tested antifungal drugs. The compound showed maximum absorption band at 250 nm. Infrared (IR) indicated presence of (NH , OH, NH) gr...
Polycyclic aromatic hydrocarbons (PAHs) in the Red sea water were determined at Suez and Ismailia... more Polycyclic aromatic hydrocarbons (PAHs) in the Red sea water were determined at Suez and Ismailia governorates, Egypt. The sites were selected to represent areas with different activities of Suez Gulf water. The concentrations of fifteen PAHs having two to six rings were determined by using HPLC with florescence detection. The total average concentrations of the fifteen PAHs over the Suez governorate sites (S1 and S2) were 1.45 and 0.34 μg/l, respectively. However those for Ismailia governorate sites (I1 and I2) were 1.06 and 0.24 μg/l, respectively. PAHs having four to six rings were the predominant compounds in particulate matters. The major sources of PAHs and their impacts on the health of certain types of fish such as Mullet and Sea-bass species were studied through the effect on the expression of stress protein related genes. In addition, expression of stress protein related genes (Cytochrome P450, CYP1A and metallothionein, MT-1A) and antioxidant enzymes (Glutathione-S-Transf...
International Journal of Scientific and Engineering Research
The Sarno River is affected by an extreme environmental degradation as a result of uncontrolled a... more The Sarno River is affected by an extreme environmental degradation as a result of uncontrolled agricultural activities and the outflow of industrial waste. Changes in the abundance indexes and biodiversity of frogs can be used as a measuring tool to determine water quality of the Sarno River. The aim of this study is to demonstrate the potential in using frog tissue as environmental indicators and to monitor changes in water quality of local streams in the development area of Sarno River. Thus, many samples are collected from sediment, water and frogs for metals analysis. To identify any differentiations of bioaccumulation of metals we have been analyzed skin and gonad of the more representative frog specie along the Sarno river, the barcoded Pelophylax bergeri. Results show that the degree of pollution in the Sarno river varies by zone, as well as by metal to metal, with contents that often are very elevated in frogs and higher in water than sediment. The concentrations of heavy m...
The aquatic environment makes up the major part of our environment and resources, therefore its s... more The aquatic environment makes up the major part of our environment and resources, therefore its safety is directly related to the safety our health. In this study, three tilapia species ( Oreochromis niloticus , Oreochromis aureus and Tilapia zilli ) and Clarias gariepinus were employed to estimate water pollution using micronucleus (MN) test. The test has been used successfully as a mutagenic assay. It is simple, reliable, sensitive, and it does not depend on any karyotypic characteristics. Fish were collected from locations that display differential environmental stresses. Two main experiments were carried out. In the first one, blood samples were collected, fixed for 24 h and then were stained with Giemsa. In the second experiment, fish were acclimated for a week. Fish were fed and each specimen had received an IP injection of cyclophosphamide (2.6, 10, 40, mg/kg b.wt). After 24 h, blood samples were collected and MN frequencies were counted and statistically tested. Results from...
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