Head of Hydrobiology department, Veterinary division, National Research CentreFemale senior professor of fish Molecular Biology and Biotechnology Address: 33, Elbehooth st., Cairo, Egypt
The aim of the present study was to investigate the effect of Lactobacillus plantarum (L. plantar... more The aim of the present study was to investigate the effect of Lactobacillus plantarum (L. plantarum) on the growth performance as well as to assess the impact of probiotics L. plantarum on the expression of immune-related genes in the liver of tilapia fish (Oreochromis niloticus). Lactobacillus plantarum was administered orally at 10⁶ CFU g⁻¹ feed to fish for 30 days. Thirty days after the start of the feeding period, fish were I/P challenged with pathogenic Pseudomonas fluorescens (0.1 ml of 10 7 cells / ml).Only the fish fed the diet containing L. plantarum showed significantly (P < 0.05) improved growth rates, protection against Pseudomonas fluorescens compared to the control. Subsequently, real-time PCR was employed to determine the mRNA levels of IL-6 TNF-α in the liver, of the control and L. plantarum groups. IL-6 and TNF-α gene expression were significantly up-regulated by L. plantarum. Moreover, the mRNA levels of IL-6and TNF-α were significantly higher in the L. plantaru...
The present study investigates the expression of A2M gene in the Oreochromis niloticus and albino... more The present study investigates the expression of A2M gene in the Oreochromis niloticus and albino rats as immune response to their infection with Zoonotic bacteria (Clostridium perfringens). The infection was established in fish with IP injection of 0.3ml of 3X108 CFU ∕ml of C. perfringens which cause Food-borne disease, and in rat rattus norvegicus by feeding on insufficient cooked infected O. niloticus, Biochemical and molecular studies were established after three and six days post challenge. Liver functions (ALT, AST and ALP) were significantly (P≤0.05) highly elevated. Kidney functions (Urea, uric acid and Calcium) were not affected but the Creatinine showed significant increase in its level (P≤0.05). CRP also was highly elevated in rats and slightly elevated in O. niloticus. The cellulose acetate gel electrophoresis of serum protein showed significant increase in the total protein and its fractions for both O. niloticus and rats in both intervals of time. Expression pattern of...
This study constitutes a survey on the Egyptian tilapia fish farms. In this study, tilapia fish (... more This study constitutes a survey on the Egyptian tilapia fish farms. In this study, tilapia fish (Oreochromis niloticus) were collected from three different fish farms in Egypt; Al-Abbasa, Kafr El-Sheikh and El-Fayoum to assess the heavy metal pollution. Cadmium (Cd), Copper (Cu), Zinc (Zn) and Lead (Pb) concentrations were measured in water and fish muscles from these studied areas. Liver enzymes, total protein levels were determined in fish serum and the expression of MT-gene was examined. Water and tissue concentrations of heavy metal recorded its highest values in Al-Abbasa followed by El-Fayoum then Kafr El-Sheikh fish farms. Analysis of GOT and GPT revealed altered values, a clear decrease in the total protein levels in association with high contamination by heavy metals was observed. Obvious increase in the expression of MT gene was observed in response to heavy metal pollution. In conclusion, this study revealed a clear heavy metal pollution in the studied Egyptian tilapia fi...
Heavy metal pollution is one of the most serious problems that face human with referring to peopl... more Heavy metal pollution is one of the most serious problems that face human with referring to people eating sea foods. Heavy metals may affect the aquatic organisms at the cellular level and possibly change some genes regulation. Metallothionein (MT) is a low molecular weight protein that binds to heavy metals in aquatic organisms and is considered as a biomarker of heavy metal pollution. Therefore, this study was undertaken to estimate the copper (Cu), cadmium (Cd), zinc (Zn) and lead (Pb) concentrations in water and fish tissues, the GOT, GPT and LDH enzymes value and the expression level of MT gene in Nile tilapia fish (Oreochromis niloticus) in Lake Manzala, Egypt. Elevated values of Cu, Cd, Zn and Pb concentrations were detected in water and fish tissues. Highly pronounced values of GOT, GPT and LDH enzymes were detected. Using RT- PCR, an evidence of increase in metallothionein expression in fish livers was detected, monitoring the fish response to heavy metal pollution in
The herbicides Thiobencarb and Dithiopyr are largely used as weed killers, especially in the rice... more The herbicides Thiobencarb and Dithiopyr are largely used as weed killers, especially in the rice fields, where cultured tilapia can be exposed to the adverse effects of such herbicides. Leaching of the herbicides in the soil could also affect the wild fish in the natural habitats and so considered as chemical water pollutants. To assess the adverse effects of these herbicides on the Nile tilapia in this study; Lcso was determined, and recorded as 720jig/l and 280jig/l for Thiobencarb and Dithiopyr respectively. Nile tilapia was exposed to acute and chronic exposures of Thiobencarb and Dithiopyr as single and mixed treatment^ 'The residue of both herbicides in fish tissues was estimated, and revealed that the highest value was detected in the liver, while the lowest was in the brain. Biochemical and genetic studies were applied to evaluate the toxicity and alterations due to Thiobencarb and Dithiopyr in Nile tilapia. bievated values ot serum and muscle total protein and alkaline...
This study was planned to investigate the effect of Enterococcus faecium probiotic on the growth ... more This study was planned to investigate the effect of Enterococcus faecium probiotic on the growth performance and to assess its impact on the expression of some immunomodulating genes in Oreochromis niloticus. E. faecium was administered orally in a level of 8g/kg feed to fish for 45 days. At the end of the feeding period, fish were I/P challenged with pathogenic Aeromonas hydrophila (0.2 ml of 2 x10 8 CFU / ml).The fish group fed the diet containing E. faecium showed significant increase in weight gain, SGR and PER. Also it showed low mortality rate, less severe clinical pictures and histopathological changes during challenge test against A.hydrophila compared to the control group. Semi-quantitative PCR was employed to determine the mRNA levels of IL-8 and TNF-α genes of the control and probiotic groups. IL-8 and TNF-α were significantly upregulated by E. faecium. Moreover these genes expression were significantly higher in the E. faecium group after A. hydrophila infection, suggest...
In the concept of global warming, the physiological mechanisms limiting and adjusting cold and he... more In the concept of global warming, the physiological mechanisms limiting and adjusting cold and heat tolerance have regained interest. Temperature is considered as one of the most important environmental stressor that can strongly affect physiological functions of fish. Therefore, to understand the role of metabolic regulation in environmental temperature stress tolerance, we have examined how different temperature acclimation (22°C and 32°C) for 7 days affects the mitochondrial properties exemplified by ATP Synthase F-type gene expression in tilapia fish Oreochromis niloticus as the cellular energy budget. The relative transcriptional activity of this gene using RTPCR a semi-quantitative analysis indicated that ATP synthase F0 gene has remarkably stable expression in response to high temperature, in contrast this gene was up-regulated in case of low temperature exposure compared to sactin gene. ATP synthase F0 was identified and characterized, ATP Synthase F0 Subunit 6 cDNA with the...
A new member of heat-shock protein family (HSP 9) identified in Tilapia Oreochromis niloticus imm... more A new member of heat-shock protein family (HSP 9) identified in Tilapia Oreochromis niloticus immunized by killed Flavobacterium columnarae. Suppressive subtractive hybridization (SSH) was utilized to construct a cDNA library and a semi-quantitative RT-PCR analysis used to examine HSP9 immune gene regulation. O. niloticus heat shock protein (ONHSP9) cDNA composed of 1228 bps with a 1167 bps open reading frame, the predicted gene product is 389 amino acid with molecular weight of 42.1 kDa, it shares 92% similarity with that of Danio rerio. Compared to s-actin, the semi- quantitative RT-PCR revealed that ONHSP9 expressed in tissues of stimulated fish as up-regulated gene suggesting that this member of heat-shock genes is probably involved in the general immune response against the pathogenic bacteria. (Nature and Science. 2009;7(2):46-57). (ISSN: 1545-0740).
This study constitutes a survey on the Egyptian tilapia fish farms. In this study, tilapia fish (... more This study constitutes a survey on the Egyptian tilapia fish farms. In this study, tilapia fish (Oreochromis niloticus) were collected from three different fish farms in Egypt; Al-Abbasa, Kafr El-Sheikh and El-Fayoum to assess the heavy metal pollution. Cadmium (Cd), Copper (Cu), Zinc (Zn) and Lead (Pb) concentrations were measured in water and fish muscles from these studied areas. Liver enzymes, total protein levels were determined in fish serum and the expression of MT-gene was examined. Water and tissue concentrations of heavy metal recorded its highest values in Al-Abbasa followed by El-Fayoum then Kafr El-Sheikh fish farms. Analysis of GOT and GPT revealed altered values, a clear decrease in the total protein levels in association with high contamination by heavy metals was observed. Obvious increase in the expression of MT gene was observed in response to heavy metal pollution. In conclusion, this study revealed a clear heavy metal pollution in the studied Egyptian tilapia fi...
BACKGROUND AND OBJECTIVE Leek (Allium ampeloprasum) is one of the most commonly used herbal foods... more BACKGROUND AND OBJECTIVE Leek (Allium ampeloprasum) is one of the most commonly used herbal foods all over the world. This study was conducted to evaluate the protective effect of leek extract on catfish experimentally challenged with Aeromonas hydrophila, a problematic bacterial pathogen that affects various freshwater fish species. MATERIALS AND METHODS Aeromonas hydrophila was isolated and identified from catfish showing clinical signs of septicemia. The in vitro activity of leek extract to control the growth of Aeromonas hydrophila was investigated. In the in vivo experiment, about 240 adult catfish (Clarias gariepinus) were fed three different leek extract concentrations (10, 25 and 50 mg kg-1 body weight) for 1 month. Later on, a challenge study was conducted using an identified A. hydrophila strain. Morbidity and mortality were recorded throughout one week post-challenge. Furthermore, the effect of leek extract on some immune-related genes was investigated. RESULTS Under the in vitro testing, a significant increase (10 and 13 mm) in the inhibition zone was recorded in wells treated with 25 and 50 mg L-1 leak extract, respectively. A significant reduction in fish mortalities was reported in all leek extract treated groups compared to the control group which was given water. TLR1 gene expression was upregulated in fish treated with leek extract while TNFα gene expression was down-regulated. CONCLUSION Overall, results suggested that the leek extract has immunostimulating effects that can help control bacterial infections in catfish and probably other fish species.
Research Journal of Pharmaceutical, Biological and Chemical Sciences
Infectious agents are potential causes of mass kills among cultured fish populations worldwide. T... more Infectious agents are potential causes of mass kills among cultured fish populations worldwide. The study was carried out through the course of two outbreaks targeted gilthead sea bream, Sparus aurata, cultured in two models of mari-culture systems at northern Egypt, floating net-cages and earthen ponds during 2012. Affected fish farms showed unique threatening seasonal episodes of high mortalities. Disease problems recorded during this period were investigated, focusing on phenotypic, molecular characterization and histopathological alterations. Total number of 100 moribund and ∕ or freshly dead fish samples showing picture of clinical septicemia were inspected through the course of these epidemics. Vibrio alginolyticus was the most predominant bacterial pathogen 28.57 % followed by streptococcus agalactiae 24.48 %, Pseudomonas fluorescens 18.36 % and Vibrio vulnificus 16.32 %. Infections attributed to Tenacibaculum maritimum recorded the lowest rate 12.24 %. PCR yielded specific a...
The aim of the present study was to investigate the effect of Lactobacillus plantarum (L. plantar... more The aim of the present study was to investigate the effect of Lactobacillus plantarum (L. plantarum) on the growth performance as well as to assess the impact of probiotics L. plantarum on the expression of immune-related genes in the liver of tilapia fish (Oreochromis niloticus). Lactobacillus plantarum was administered orally at 10⁶ CFU g⁻¹ feed to fish for 30 days. Thirty days after the start of the feeding period, fish were I/P challenged with pathogenic Pseudomonas fluorescens (0.1 ml of 10 7 cells / ml).Only the fish fed the diet containing L. plantarum showed significantly (P < 0.05) improved growth rates, protection against Pseudomonas fluorescens compared to the control. Subsequently, real-time PCR was employed to determine the mRNA levels of IL-6 TNF-α in the liver, of the control and L. plantarum groups. IL-6 and TNF-α gene expression were significantly up-regulated by L. plantarum. Moreover, the mRNA levels of IL-6and TNF-α were significantly higher in the L. plantaru...
The present study investigates the expression of A2M gene in the Oreochromis niloticus and albino... more The present study investigates the expression of A2M gene in the Oreochromis niloticus and albino rats as immune response to their infection with Zoonotic bacteria (Clostridium perfringens). The infection was established in fish with IP injection of 0.3ml of 3X108 CFU ∕ml of C. perfringens which cause Food-borne disease, and in rat rattus norvegicus by feeding on insufficient cooked infected O. niloticus, Biochemical and molecular studies were established after three and six days post challenge. Liver functions (ALT, AST and ALP) were significantly (P≤0.05) highly elevated. Kidney functions (Urea, uric acid and Calcium) were not affected but the Creatinine showed significant increase in its level (P≤0.05). CRP also was highly elevated in rats and slightly elevated in O. niloticus. The cellulose acetate gel electrophoresis of serum protein showed significant increase in the total protein and its fractions for both O. niloticus and rats in both intervals of time. Expression pattern of...
This study constitutes a survey on the Egyptian tilapia fish farms. In this study, tilapia fish (... more This study constitutes a survey on the Egyptian tilapia fish farms. In this study, tilapia fish (Oreochromis niloticus) were collected from three different fish farms in Egypt; Al-Abbasa, Kafr El-Sheikh and El-Fayoum to assess the heavy metal pollution. Cadmium (Cd), Copper (Cu), Zinc (Zn) and Lead (Pb) concentrations were measured in water and fish muscles from these studied areas. Liver enzymes, total protein levels were determined in fish serum and the expression of MT-gene was examined. Water and tissue concentrations of heavy metal recorded its highest values in Al-Abbasa followed by El-Fayoum then Kafr El-Sheikh fish farms. Analysis of GOT and GPT revealed altered values, a clear decrease in the total protein levels in association with high contamination by heavy metals was observed. Obvious increase in the expression of MT gene was observed in response to heavy metal pollution. In conclusion, this study revealed a clear heavy metal pollution in the studied Egyptian tilapia fi...
Heavy metal pollution is one of the most serious problems that face human with referring to peopl... more Heavy metal pollution is one of the most serious problems that face human with referring to people eating sea foods. Heavy metals may affect the aquatic organisms at the cellular level and possibly change some genes regulation. Metallothionein (MT) is a low molecular weight protein that binds to heavy metals in aquatic organisms and is considered as a biomarker of heavy metal pollution. Therefore, this study was undertaken to estimate the copper (Cu), cadmium (Cd), zinc (Zn) and lead (Pb) concentrations in water and fish tissues, the GOT, GPT and LDH enzymes value and the expression level of MT gene in Nile tilapia fish (Oreochromis niloticus) in Lake Manzala, Egypt. Elevated values of Cu, Cd, Zn and Pb concentrations were detected in water and fish tissues. Highly pronounced values of GOT, GPT and LDH enzymes were detected. Using RT- PCR, an evidence of increase in metallothionein expression in fish livers was detected, monitoring the fish response to heavy metal pollution in
The herbicides Thiobencarb and Dithiopyr are largely used as weed killers, especially in the rice... more The herbicides Thiobencarb and Dithiopyr are largely used as weed killers, especially in the rice fields, where cultured tilapia can be exposed to the adverse effects of such herbicides. Leaching of the herbicides in the soil could also affect the wild fish in the natural habitats and so considered as chemical water pollutants. To assess the adverse effects of these herbicides on the Nile tilapia in this study; Lcso was determined, and recorded as 720jig/l and 280jig/l for Thiobencarb and Dithiopyr respectively. Nile tilapia was exposed to acute and chronic exposures of Thiobencarb and Dithiopyr as single and mixed treatment^ 'The residue of both herbicides in fish tissues was estimated, and revealed that the highest value was detected in the liver, while the lowest was in the brain. Biochemical and genetic studies were applied to evaluate the toxicity and alterations due to Thiobencarb and Dithiopyr in Nile tilapia. bievated values ot serum and muscle total protein and alkaline...
This study was planned to investigate the effect of Enterococcus faecium probiotic on the growth ... more This study was planned to investigate the effect of Enterococcus faecium probiotic on the growth performance and to assess its impact on the expression of some immunomodulating genes in Oreochromis niloticus. E. faecium was administered orally in a level of 8g/kg feed to fish for 45 days. At the end of the feeding period, fish were I/P challenged with pathogenic Aeromonas hydrophila (0.2 ml of 2 x10 8 CFU / ml).The fish group fed the diet containing E. faecium showed significant increase in weight gain, SGR and PER. Also it showed low mortality rate, less severe clinical pictures and histopathological changes during challenge test against A.hydrophila compared to the control group. Semi-quantitative PCR was employed to determine the mRNA levels of IL-8 and TNF-α genes of the control and probiotic groups. IL-8 and TNF-α were significantly upregulated by E. faecium. Moreover these genes expression were significantly higher in the E. faecium group after A. hydrophila infection, suggest...
In the concept of global warming, the physiological mechanisms limiting and adjusting cold and he... more In the concept of global warming, the physiological mechanisms limiting and adjusting cold and heat tolerance have regained interest. Temperature is considered as one of the most important environmental stressor that can strongly affect physiological functions of fish. Therefore, to understand the role of metabolic regulation in environmental temperature stress tolerance, we have examined how different temperature acclimation (22°C and 32°C) for 7 days affects the mitochondrial properties exemplified by ATP Synthase F-type gene expression in tilapia fish Oreochromis niloticus as the cellular energy budget. The relative transcriptional activity of this gene using RTPCR a semi-quantitative analysis indicated that ATP synthase F0 gene has remarkably stable expression in response to high temperature, in contrast this gene was up-regulated in case of low temperature exposure compared to sactin gene. ATP synthase F0 was identified and characterized, ATP Synthase F0 Subunit 6 cDNA with the...
A new member of heat-shock protein family (HSP 9) identified in Tilapia Oreochromis niloticus imm... more A new member of heat-shock protein family (HSP 9) identified in Tilapia Oreochromis niloticus immunized by killed Flavobacterium columnarae. Suppressive subtractive hybridization (SSH) was utilized to construct a cDNA library and a semi-quantitative RT-PCR analysis used to examine HSP9 immune gene regulation. O. niloticus heat shock protein (ONHSP9) cDNA composed of 1228 bps with a 1167 bps open reading frame, the predicted gene product is 389 amino acid with molecular weight of 42.1 kDa, it shares 92% similarity with that of Danio rerio. Compared to s-actin, the semi- quantitative RT-PCR revealed that ONHSP9 expressed in tissues of stimulated fish as up-regulated gene suggesting that this member of heat-shock genes is probably involved in the general immune response against the pathogenic bacteria. (Nature and Science. 2009;7(2):46-57). (ISSN: 1545-0740).
This study constitutes a survey on the Egyptian tilapia fish farms. In this study, tilapia fish (... more This study constitutes a survey on the Egyptian tilapia fish farms. In this study, tilapia fish (Oreochromis niloticus) were collected from three different fish farms in Egypt; Al-Abbasa, Kafr El-Sheikh and El-Fayoum to assess the heavy metal pollution. Cadmium (Cd), Copper (Cu), Zinc (Zn) and Lead (Pb) concentrations were measured in water and fish muscles from these studied areas. Liver enzymes, total protein levels were determined in fish serum and the expression of MT-gene was examined. Water and tissue concentrations of heavy metal recorded its highest values in Al-Abbasa followed by El-Fayoum then Kafr El-Sheikh fish farms. Analysis of GOT and GPT revealed altered values, a clear decrease in the total protein levels in association with high contamination by heavy metals was observed. Obvious increase in the expression of MT gene was observed in response to heavy metal pollution. In conclusion, this study revealed a clear heavy metal pollution in the studied Egyptian tilapia fi...
BACKGROUND AND OBJECTIVE Leek (Allium ampeloprasum) is one of the most commonly used herbal foods... more BACKGROUND AND OBJECTIVE Leek (Allium ampeloprasum) is one of the most commonly used herbal foods all over the world. This study was conducted to evaluate the protective effect of leek extract on catfish experimentally challenged with Aeromonas hydrophila, a problematic bacterial pathogen that affects various freshwater fish species. MATERIALS AND METHODS Aeromonas hydrophila was isolated and identified from catfish showing clinical signs of septicemia. The in vitro activity of leek extract to control the growth of Aeromonas hydrophila was investigated. In the in vivo experiment, about 240 adult catfish (Clarias gariepinus) were fed three different leek extract concentrations (10, 25 and 50 mg kg-1 body weight) for 1 month. Later on, a challenge study was conducted using an identified A. hydrophila strain. Morbidity and mortality were recorded throughout one week post-challenge. Furthermore, the effect of leek extract on some immune-related genes was investigated. RESULTS Under the in vitro testing, a significant increase (10 and 13 mm) in the inhibition zone was recorded in wells treated with 25 and 50 mg L-1 leak extract, respectively. A significant reduction in fish mortalities was reported in all leek extract treated groups compared to the control group which was given water. TLR1 gene expression was upregulated in fish treated with leek extract while TNFα gene expression was down-regulated. CONCLUSION Overall, results suggested that the leek extract has immunostimulating effects that can help control bacterial infections in catfish and probably other fish species.
Research Journal of Pharmaceutical, Biological and Chemical Sciences
Infectious agents are potential causes of mass kills among cultured fish populations worldwide. T... more Infectious agents are potential causes of mass kills among cultured fish populations worldwide. The study was carried out through the course of two outbreaks targeted gilthead sea bream, Sparus aurata, cultured in two models of mari-culture systems at northern Egypt, floating net-cages and earthen ponds during 2012. Affected fish farms showed unique threatening seasonal episodes of high mortalities. Disease problems recorded during this period were investigated, focusing on phenotypic, molecular characterization and histopathological alterations. Total number of 100 moribund and ∕ or freshly dead fish samples showing picture of clinical septicemia were inspected through the course of these epidemics. Vibrio alginolyticus was the most predominant bacterial pathogen 28.57 % followed by streptococcus agalactiae 24.48 %, Pseudomonas fluorescens 18.36 % and Vibrio vulnificus 16.32 %. Infections attributed to Tenacibaculum maritimum recorded the lowest rate 12.24 %. PCR yielded specific a...
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