Whole genome duplication (WGD) is an evolutionary event resulting in a redundancy of genetic mate... more Whole genome duplication (WGD) is an evolutionary event resulting in a redundancy of genetic material. Different mechanisms of genome doubling through allo- or autopolyploidization could lead to distinct evolutionary trajectories of newly formed polyploids. Genome studies on such species are undoubtedly important for understanding one of the crucial stages of genome evolution. However, assembling neopolyploid appears to be a challenging task because its genome consists of two homologous (or homeologous) chromosome sets and therefore contains the extended paralogous regions with a high homology level. Post-WGD evolution of polyploids includes rediploidization, first part of which is cytogenetic diploidization led to the formation of species, whose polyploid origin might be hidden by disomic inheritance and diploid-like meiosis. Earlier we uncovered the hidden polyploid origin of free-living flatworms of the genusMacrostomum(Macrostomum lignano, M. janickei, andM. mirumnovem). Despite...
The gene composition, function and evolution of B-chromosomes (Bs) have been actively discussed i... more The gene composition, function and evolution of B-chromosomes (Bs) have been actively discussed in recent years. However, the additional genomic elements are still enigmatic. One of Bs mysteries is their spatial organization in the interphase nucleus. It is known that heterochromatic compartments are not randomly localized in a nucleus. The purpose of this work was to study the organization and three-dimensional spatial arrangement of Bs in the interphase nucleus. Using microdissection of Bs and autosome centromeric heterochromatic regions of the yellow-necked mouse (Apodemus flavicollis) we obtained DNA probes for further two-dimensional (2D)- and three-dimensional (3D)- fluorescence in situ hybridization (FISH) studies. Simultaneous in situ hybridization of obtained here B-specific DNA probes and autosomal C-positive pericentromeric region-specific probes further corroborated the previously stated hypothesis about the pseudoautosomal origin of the additional chromosomes of this sp...
The results of comprehensive clinical examination and molecular cytogenetic analysis of a patient... more The results of comprehensive clinical examination and molecular cytogenetic analysis of a patient carrying chromosome 3p+ in 69% of the peripheral blood lymphocytes are presented. Using microdissection of the metaphase chromosomes followed by DOP–PCR, a DNA library specific for the abnormal chromosome was obtained. By fluorescence in situ hybridization (FISH) of this DNA library on chromosomes from the patient and a healthy donor, the aberrant chromosome was identified as der(3)t(3;10)(p25;q24.3). Since this chromosome was present in only a proportion of patient's cells studied and no chromosome aberrations were revealed in cells of his parents, the der(3)t(3;10) is suggested to appear de novo. The cells carrying der(3)t(3;10) are monosomic for a proportion of 3p25 and trisomic for 10q24.3 → qter. The developmental malformations revealed in the patient, such as the specific features of facial skeleton, mental retardation, microcephaly, and others are similar to those described p...
7th Korea-Russia International Symposium on Science and Technology, Proceedings KORUS 2003. (IEEE Cat. No.03EX737), 2003
The high efficiency of chromosome analysis in human inherited pathology, neoplastic cell transfor... more The high efficiency of chromosome analysis in human inherited pathology, neoplastic cell transformation, and karyotypic evolution in mammals is associated with application of numerous DNA probes in fluorescent in situ hybridization (FISH). Generation of the whole chromosome paints specific to the abnormal chromosomes or chromosome regions followed with reversed painting provides detailed information that allows making final diagnosis or determining the direction of further investigation. The technique for generating painting probes with metaphase chromosome microdissection and degenerate oligonucleotideprimed polymerase chain reaction (DOP-PCR) was developed and used for analysis in cases of small supernumerary marker chromosomes, unbalance translocations, deletions, and complex chromosome rearrangements. The efficiency of this approach was compared with that of multiplex FISH, spectral karyotyping, and multicolor banding. In many cases the results of chromosome microdissection foll...
A possibility of obtaining a panel of marsupial-rodent somatic cell hybrid clones has been explor... more A possibility of obtaining a panel of marsupial-rodent somatic cell hybrid clones has been explored, with a view to mapping the genome of the opossum (Monodelphis domestica). Fusion of opossum cells (splenocytes, bone marrow cells, and fibroblasts) with fibroblasts of Chinese hamster or vole (HGPRT- and TK- mutants, respectively) produced 146 hybrid clones. The majority of marsupial-mammalian somatic cell hybrids were characterized by pronounced fragmentation and segregation of marsupial chromosomes. To overcome this difficulty, a method for rapid screening was developed, which allowed the early selection of clones rich in chromosomal material of opossum. Based on the screening results, 25 clones of independent origin were selected. A detailed genetic analysis, which included chromosome G-banding and in situ hybridization of biotin-labeled opossum DNA on metaphase chromosomes, allowed further selection of seven hybrid clones containing one to six intact chromosomes of M. domestica. ...
Evidence is presented for the chromosome localization of seven silver fox genes obtained with the... more Evidence is presented for the chromosome localization of seven silver fox genes obtained with the help of panel of fox x Chinese hamster somatic cell hybrids. Thus, the AK1, GOT2 and ALDOC are assigned to chromosome VFU2, PGP to chromosome VFU3, BLVR to chromosome VFU5, ACP1 to chromosome VFU8 and ITPA to chromosome VFU14. The genetic map of 29 fox genes is compared with those reported for man and other animals. The results obtained support and extend our previous suggestion that formation of the Canidae branch of the Carnivora phylogenetic tree was associated with great increase in the rate of reorganization of the ancestral karyotype.
Abstract book of the 2nd International Scientific Conference "Plants and Microbes: the Future of Biotechnology" PLAMIC2020, 2020
The present report is devoted to analysis of results obtained with modern molecular and molecular... more The present report is devoted to analysis of results obtained with modern molecular and molecular-cytogenetic methods in studies of karyotype and genome organization in various animal species. Perspectives of their application for the study of karyotype and genome organization in plants are considered and discussed.
Whole genome duplication (WGD) is an evolutionary event resulting in a redundancy of genetic mate... more Whole genome duplication (WGD) is an evolutionary event resulting in a redundancy of genetic material. Different mechanisms of genome doubling through allo- or autopolyploidization could lead to distinct evolutionary trajectories of newly formed polyploids. Genome studies on such species are undoubtedly important for understanding one of the crucial stages of genome evolution. However, assembling neopolyploid appears to be a challenging task because its genome consists of two homologous (or homeologous) chromosome sets and therefore contains the extended paralogous regions with a high homology level. Post-WGD evolution of polyploids includes rediploidization, first part of which is cytogenetic diploidization led to the formation of species, whose polyploid origin might be hidden by disomic inheritance and diploid-like meiosis. Earlier we uncovered the hidden polyploid origin of free-living flatworms of the genusMacrostomum(Macrostomum lignano, M. janickei, andM. mirumnovem). Despite...
The gene composition, function and evolution of B-chromosomes (Bs) have been actively discussed i... more The gene composition, function and evolution of B-chromosomes (Bs) have been actively discussed in recent years. However, the additional genomic elements are still enigmatic. One of Bs mysteries is their spatial organization in the interphase nucleus. It is known that heterochromatic compartments are not randomly localized in a nucleus. The purpose of this work was to study the organization and three-dimensional spatial arrangement of Bs in the interphase nucleus. Using microdissection of Bs and autosome centromeric heterochromatic regions of the yellow-necked mouse (Apodemus flavicollis) we obtained DNA probes for further two-dimensional (2D)- and three-dimensional (3D)- fluorescence in situ hybridization (FISH) studies. Simultaneous in situ hybridization of obtained here B-specific DNA probes and autosomal C-positive pericentromeric region-specific probes further corroborated the previously stated hypothesis about the pseudoautosomal origin of the additional chromosomes of this sp...
The results of comprehensive clinical examination and molecular cytogenetic analysis of a patient... more The results of comprehensive clinical examination and molecular cytogenetic analysis of a patient carrying chromosome 3p+ in 69% of the peripheral blood lymphocytes are presented. Using microdissection of the metaphase chromosomes followed by DOP–PCR, a DNA library specific for the abnormal chromosome was obtained. By fluorescence in situ hybridization (FISH) of this DNA library on chromosomes from the patient and a healthy donor, the aberrant chromosome was identified as der(3)t(3;10)(p25;q24.3). Since this chromosome was present in only a proportion of patient's cells studied and no chromosome aberrations were revealed in cells of his parents, the der(3)t(3;10) is suggested to appear de novo. The cells carrying der(3)t(3;10) are monosomic for a proportion of 3p25 and trisomic for 10q24.3 → qter. The developmental malformations revealed in the patient, such as the specific features of facial skeleton, mental retardation, microcephaly, and others are similar to those described p...
7th Korea-Russia International Symposium on Science and Technology, Proceedings KORUS 2003. (IEEE Cat. No.03EX737), 2003
The high efficiency of chromosome analysis in human inherited pathology, neoplastic cell transfor... more The high efficiency of chromosome analysis in human inherited pathology, neoplastic cell transformation, and karyotypic evolution in mammals is associated with application of numerous DNA probes in fluorescent in situ hybridization (FISH). Generation of the whole chromosome paints specific to the abnormal chromosomes or chromosome regions followed with reversed painting provides detailed information that allows making final diagnosis or determining the direction of further investigation. The technique for generating painting probes with metaphase chromosome microdissection and degenerate oligonucleotideprimed polymerase chain reaction (DOP-PCR) was developed and used for analysis in cases of small supernumerary marker chromosomes, unbalance translocations, deletions, and complex chromosome rearrangements. The efficiency of this approach was compared with that of multiplex FISH, spectral karyotyping, and multicolor banding. In many cases the results of chromosome microdissection foll...
A possibility of obtaining a panel of marsupial-rodent somatic cell hybrid clones has been explor... more A possibility of obtaining a panel of marsupial-rodent somatic cell hybrid clones has been explored, with a view to mapping the genome of the opossum (Monodelphis domestica). Fusion of opossum cells (splenocytes, bone marrow cells, and fibroblasts) with fibroblasts of Chinese hamster or vole (HGPRT- and TK- mutants, respectively) produced 146 hybrid clones. The majority of marsupial-mammalian somatic cell hybrids were characterized by pronounced fragmentation and segregation of marsupial chromosomes. To overcome this difficulty, a method for rapid screening was developed, which allowed the early selection of clones rich in chromosomal material of opossum. Based on the screening results, 25 clones of independent origin were selected. A detailed genetic analysis, which included chromosome G-banding and in situ hybridization of biotin-labeled opossum DNA on metaphase chromosomes, allowed further selection of seven hybrid clones containing one to six intact chromosomes of M. domestica. ...
Evidence is presented for the chromosome localization of seven silver fox genes obtained with the... more Evidence is presented for the chromosome localization of seven silver fox genes obtained with the help of panel of fox x Chinese hamster somatic cell hybrids. Thus, the AK1, GOT2 and ALDOC are assigned to chromosome VFU2, PGP to chromosome VFU3, BLVR to chromosome VFU5, ACP1 to chromosome VFU8 and ITPA to chromosome VFU14. The genetic map of 29 fox genes is compared with those reported for man and other animals. The results obtained support and extend our previous suggestion that formation of the Canidae branch of the Carnivora phylogenetic tree was associated with great increase in the rate of reorganization of the ancestral karyotype.
Abstract book of the 2nd International Scientific Conference "Plants and Microbes: the Future of Biotechnology" PLAMIC2020, 2020
The present report is devoted to analysis of results obtained with modern molecular and molecular... more The present report is devoted to analysis of results obtained with modern molecular and molecular-cytogenetic methods in studies of karyotype and genome organization in various animal species. Perspectives of their application for the study of karyotype and genome organization in plants are considered and discussed.
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