Additional file 3: Table S2. Data on the 722 pig D-loop sequences analyzed in this study. Europea... more Additional file 3: Table S2. Data on the 722 pig D-loop sequences analyzed in this study. European, African and Asian mitochondrial control region sequences retrieved from GenBank were used for the median joining network analysis (Fig. 1). Assignments to sub-haplogroups and variants conform to the pig and wild boar mtDNA tree obtained from DomeTree [22].
Additional file 1: Table S1. Data on the 204 hairy black NIP sampled from six states in Nigeria. ... more Additional file 1: Table S1. Data on the 204 hairy black NIP sampled from six states in Nigeria. Information on the mtDNA, Y-chromosome and MC1R of the 204 NIP samples.
In this study, the preparation, characterization, and evaluation of the cross-linked enzyme aggre... more In this study, the preparation, characterization, and evaluation of the cross-linked enzyme aggregates (CLEAs) of fructosyltransferase isolated from the mutant-type Aureobasidium pullulans NAC8 for the production of fructooligosaccharides (FOS) with prebiotic properties were investigated. The reaction conditions were optimized statistically by using response surface methodology. CLEAs were characterized by using both photo-microscopy and scanning electron microscopy energy-dispersive X-ray spectroscopy (SEM-EDX). The secondary structure of the protein was predicted by using Fourier transform infrared spectroscopy (FTIR). FOSs produced as a result of the biotransformation of sucrose was quantified by using a chemometric analysis of FTIR spectral data. The prepared FOSs were investigated for the prebiotic effects under anaerobic conditions using a standard strain of Lactobacillus sp. Following were the statistically determined, optimum conditions for the production of FOSs: 5% (v/v) g...
Various methods for the detection and analysis of genetic polymorphisms at the DNA-level have bee... more Various methods for the detection and analysis of genetic polymorphisms at the DNA-level have been developed. Notable polymorphisms may be found in the mitochondrial DNA and the minisatellites and microsatellites of genomic DNA. Such polymorphisms are exceedingly numerous and may provide a promising basis for a number of scientific as well as practical applications in the genetic studies in Tilapia. An expanded number of genetic markers would increase statistical insights on marker-based population studies which are highly relevant in the assessment and improvement of commercially important stocks of Tilapia. In addition, utilization of these genetic markers could contribute to the rapid elaboration of piscine genomic maps and to the development of markers for health and production-related traits of fish.
The application of biotechnology-based products to respond to critical needs in the agrifood and ... more The application of biotechnology-based products to respond to critical needs in the agrifood and environmental management sectors of developing countries is an integrated set of activities designed to identify opportunities for biotechnolog ical innovations, and to overcome key bottlenecks to their effective application. This decision reflects recognition of the need for careful, rigorous analysis of the social, economic, and environmental impacts of agricultural biotechnology applications. From the outset, it was felt that this should be a crucial p art of the overall work program, an essential complement to activities geared to promoting the tr ansfer and application of specific biotechnology-based products, and improving the capacities of biotechnology-based enterprises. Understanding the way in which biotechnology applications affect socioeconomic and environmental variables is not simply an academic e xercise. Impact assessment data is important to a range of decision-makers: f...
BackgroundThe history of pig populations in Africa remains controversial due to insufficient evid... more BackgroundThe history of pig populations in Africa remains controversial due to insufficient evidence from archaeological and genetic data. Previously, a Western ancestry for West African pigs was reported based on loci that are involved in the determination of coat color. We investigated the genetic diversity of Nigerian indigenous pigs (NIP) by simultaneously analyzing variation in mitochondrial DNA (mtDNA), Y-chromosome sequence and the melanocortin receptor 1 (MC1R) gene.ResultsMedian-joining network analysis of mtDNA D-loop sequences from 201 NIP and previously characterized loci clustered NIP with populations from the West (Europe/North Africa) and East/Southeast Asia. Analysis of partial sequences of the Y-chromosome in 57 Nigerian boars clustered NIP into lineage HY1. Finally, analysis of MC1R in 90 NIP resulted in seven haplotypes, among which the European wild boar haplotype was carried by one individual and the European dominant black by most of the other individuals (93%...
The history of African pig breeds is still controversial due to the relative scarcity of archaeol... more The history of African pig breeds is still controversial due to the relative scarcity of archaeological and genetic data clarifying their origins. While these breeds might descend from Near Eastern pigs entering the continent via Egypt during the Neolithic, Africa may be a primary domestication locus for pigs, as indicated for other species such as cattle and donkeys. In this regard, characterisation of the mitochondrial gene pool of African pig breeds has revealed a very low frequency of Near Eastern alleles, suggesting that, if Fertile Crescent pigs played a part in the foundation of African breeds, their genetic signature has been substantially erased. Interestingly, genetic analysis of western and eastern African pig breeds has revealed a strong phylogeographic pattern, with the latter harbouring Far Eastern alleles at high frequencies. This finding is consistent with data obtained for chickens and confirms that livestock was transported in ancient times, from the Far East to Af...
This work studied the chromosomal structure of the Nigerian indigenous pig (NIP). The karyotype w... more This work studied the chromosomal structure of the Nigerian indigenous pig (NIP). The karyotype was obtained and a detailed karyogram constructed. Using sixty-two metaphases of the NIP obtained from both mature male and female leucocytes culture, the chromosomal structure was studied. The karyotype obtained was based on the chromosome’s relative length, centromere position and arm (p/q) ratio. The cells had the usual diploid complement of 38 chromosomes (2n = 38) and a nombre fondamental of 64 as in the exotic. The karyotype of NIP was characterized by submetacentric chromosome pairs 1 – 7; metacentric chromosome pairs 8 to 12 and telocentric chromosome pairs 13 to 18 with submetacentric XX for female and XY for male. A detailed karyogram was constructed from the obtained results.
Genetics, selection, evolution : GSE, Jun 26, 2017
The history of pig populations in Africa remains controversial due to insufficient evidence from ... more The history of pig populations in Africa remains controversial due to insufficient evidence from archaeological and genetic data. Previously, a Western ancestry for West African pigs was reported based on loci that are involved in the determination of coat color. We investigated the genetic diversity of Nigerian indigenous pigs (NIP) by simultaneously analyzing variation in mitochondrial DNA (mtDNA), Y-chromosome sequence and the melanocortin receptor 1 (MC1R) gene. Median-joining network analysis of mtDNA D-loop sequences from 201 NIP and previously characterized loci clustered NIP with populations from the West (Europe/North Africa) and East/Southeast Asia. Analysis of partial sequences of the Y-chromosome in 57 Nigerian boars clustered NIP into lineage HY1. Finally, analysis of MC1R in 90 NIP resulted in seven haplotypes, among which the European wild boar haplotype was carried by one individual and the European dominant black by most of the other individuals (93%). The five rema...
Additional file 3: Table S2. Data on the 722 pig D-loop sequences analyzed in this study. Europea... more Additional file 3: Table S2. Data on the 722 pig D-loop sequences analyzed in this study. European, African and Asian mitochondrial control region sequences retrieved from GenBank were used for the median joining network analysis (Fig. 1). Assignments to sub-haplogroups and variants conform to the pig and wild boar mtDNA tree obtained from DomeTree [22].
Additional file 1: Table S1. Data on the 204 hairy black NIP sampled from six states in Nigeria. ... more Additional file 1: Table S1. Data on the 204 hairy black NIP sampled from six states in Nigeria. Information on the mtDNA, Y-chromosome and MC1R of the 204 NIP samples.
In this study, the preparation, characterization, and evaluation of the cross-linked enzyme aggre... more In this study, the preparation, characterization, and evaluation of the cross-linked enzyme aggregates (CLEAs) of fructosyltransferase isolated from the mutant-type Aureobasidium pullulans NAC8 for the production of fructooligosaccharides (FOS) with prebiotic properties were investigated. The reaction conditions were optimized statistically by using response surface methodology. CLEAs were characterized by using both photo-microscopy and scanning electron microscopy energy-dispersive X-ray spectroscopy (SEM-EDX). The secondary structure of the protein was predicted by using Fourier transform infrared spectroscopy (FTIR). FOSs produced as a result of the biotransformation of sucrose was quantified by using a chemometric analysis of FTIR spectral data. The prepared FOSs were investigated for the prebiotic effects under anaerobic conditions using a standard strain of Lactobacillus sp. Following were the statistically determined, optimum conditions for the production of FOSs: 5% (v/v) g...
Various methods for the detection and analysis of genetic polymorphisms at the DNA-level have bee... more Various methods for the detection and analysis of genetic polymorphisms at the DNA-level have been developed. Notable polymorphisms may be found in the mitochondrial DNA and the minisatellites and microsatellites of genomic DNA. Such polymorphisms are exceedingly numerous and may provide a promising basis for a number of scientific as well as practical applications in the genetic studies in Tilapia. An expanded number of genetic markers would increase statistical insights on marker-based population studies which are highly relevant in the assessment and improvement of commercially important stocks of Tilapia. In addition, utilization of these genetic markers could contribute to the rapid elaboration of piscine genomic maps and to the development of markers for health and production-related traits of fish.
The application of biotechnology-based products to respond to critical needs in the agrifood and ... more The application of biotechnology-based products to respond to critical needs in the agrifood and environmental management sectors of developing countries is an integrated set of activities designed to identify opportunities for biotechnolog ical innovations, and to overcome key bottlenecks to their effective application. This decision reflects recognition of the need for careful, rigorous analysis of the social, economic, and environmental impacts of agricultural biotechnology applications. From the outset, it was felt that this should be a crucial p art of the overall work program, an essential complement to activities geared to promoting the tr ansfer and application of specific biotechnology-based products, and improving the capacities of biotechnology-based enterprises. Understanding the way in which biotechnology applications affect socioeconomic and environmental variables is not simply an academic e xercise. Impact assessment data is important to a range of decision-makers: f...
BackgroundThe history of pig populations in Africa remains controversial due to insufficient evid... more BackgroundThe history of pig populations in Africa remains controversial due to insufficient evidence from archaeological and genetic data. Previously, a Western ancestry for West African pigs was reported based on loci that are involved in the determination of coat color. We investigated the genetic diversity of Nigerian indigenous pigs (NIP) by simultaneously analyzing variation in mitochondrial DNA (mtDNA), Y-chromosome sequence and the melanocortin receptor 1 (MC1R) gene.ResultsMedian-joining network analysis of mtDNA D-loop sequences from 201 NIP and previously characterized loci clustered NIP with populations from the West (Europe/North Africa) and East/Southeast Asia. Analysis of partial sequences of the Y-chromosome in 57 Nigerian boars clustered NIP into lineage HY1. Finally, analysis of MC1R in 90 NIP resulted in seven haplotypes, among which the European wild boar haplotype was carried by one individual and the European dominant black by most of the other individuals (93%...
The history of African pig breeds is still controversial due to the relative scarcity of archaeol... more The history of African pig breeds is still controversial due to the relative scarcity of archaeological and genetic data clarifying their origins. While these breeds might descend from Near Eastern pigs entering the continent via Egypt during the Neolithic, Africa may be a primary domestication locus for pigs, as indicated for other species such as cattle and donkeys. In this regard, characterisation of the mitochondrial gene pool of African pig breeds has revealed a very low frequency of Near Eastern alleles, suggesting that, if Fertile Crescent pigs played a part in the foundation of African breeds, their genetic signature has been substantially erased. Interestingly, genetic analysis of western and eastern African pig breeds has revealed a strong phylogeographic pattern, with the latter harbouring Far Eastern alleles at high frequencies. This finding is consistent with data obtained for chickens and confirms that livestock was transported in ancient times, from the Far East to Af...
This work studied the chromosomal structure of the Nigerian indigenous pig (NIP). The karyotype w... more This work studied the chromosomal structure of the Nigerian indigenous pig (NIP). The karyotype was obtained and a detailed karyogram constructed. Using sixty-two metaphases of the NIP obtained from both mature male and female leucocytes culture, the chromosomal structure was studied. The karyotype obtained was based on the chromosome’s relative length, centromere position and arm (p/q) ratio. The cells had the usual diploid complement of 38 chromosomes (2n = 38) and a nombre fondamental of 64 as in the exotic. The karyotype of NIP was characterized by submetacentric chromosome pairs 1 – 7; metacentric chromosome pairs 8 to 12 and telocentric chromosome pairs 13 to 18 with submetacentric XX for female and XY for male. A detailed karyogram was constructed from the obtained results.
Genetics, selection, evolution : GSE, Jun 26, 2017
The history of pig populations in Africa remains controversial due to insufficient evidence from ... more The history of pig populations in Africa remains controversial due to insufficient evidence from archaeological and genetic data. Previously, a Western ancestry for West African pigs was reported based on loci that are involved in the determination of coat color. We investigated the genetic diversity of Nigerian indigenous pigs (NIP) by simultaneously analyzing variation in mitochondrial DNA (mtDNA), Y-chromosome sequence and the melanocortin receptor 1 (MC1R) gene. Median-joining network analysis of mtDNA D-loop sequences from 201 NIP and previously characterized loci clustered NIP with populations from the West (Europe/North Africa) and East/Southeast Asia. Analysis of partial sequences of the Y-chromosome in 57 Nigerian boars clustered NIP into lineage HY1. Finally, analysis of MC1R in 90 NIP resulted in seven haplotypes, among which the European wild boar haplotype was carried by one individual and the European dominant black by most of the other individuals (93%). The five rema...
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Papers by Ofelia Omitogun, Prof