Background & objective: Genetic polymorphisms in the vitamin D receptor (VDR) gene are related to... more Background & objective: Genetic polymorphisms in the vitamin D receptor (VDR) gene are related to bone mineral density, bone turnover, and diseases with bone loss. Alveolar bone loss is a key feature in periodontitis. The aim of this study was to determine whether severe generalized chronic periodontitis (CP) in a Turkish population was associated with polymorphisms in the VDR gene. Methods: Samples of venous blood and DNA were obtained from 72 patients with severe generalized chronic periodontitis and 102 healthy controls. The polymorphic regions were amplified using PCR followed by digestion with restriction enzymes BsmI A/G(rs1544410), ApaI G/T(rs11168271), TaqI T/C(rs731236), and analyzed electrophoretically. Genotype and allele frequencies were calculated. Results: There were no statistically significant differences in the frequencies of VDR BsmI, ApaI, TaqI genotypes between the CP patients and healthy controls. The GTT haplotype, constructed from the three adjacent restriction fragment length polymorphisms was found to be over-represented among CP cases. This corresponded an OR of 2.4 (95% confidence interval, 1.12-5.18) for heterozygous carriers and 2.27 (95% confidence interval, 0.95-5.4) for homozygous carrier of the risk haplotype. Interpretation & conclusion: The present findings indicated that BsmI, ApaI, TaqI polymorphisms of the VDR gene were not associated with the severe generalized CP in the studied Turkish patients. Moreover, the VDR genotypes based on haplotype analysis may be associated with chronic periodontitis. In the future, diagnostic periodontal risk assessments like polymorphisms may be useful in detection of individuals susceptible for periodontitis.
Comparison of The Myobacterium Tuberculosis with The Conventional Methods Polymerase chain reacti... more Comparison of The Myobacterium Tuberculosis with The Conventional Methods Polymerase chain reaction (PCR). due to its faster response than culture was used for the diagnosis of Mycobacterium tuberculosis. Amplification reactions were performed using primers that amplify a 123 bp fragment of Insertion Sequence 6110 (IS61 10) specific to M. tuberculosis. A total of 64 samples were tested by PCR. The type (number) of specimens tested as fallows: sputum (31). urine (20). perisentez (5), torosentez (4). peritoneal fluid (3) and broneial washing (1). Sputum samples were decontaminated by N-acetyl-L-cysteine-NaOH. other samples were decontaminated by NaOH-Na citrate method. The DNA used in PCR was obtained by boiling. 33 samples were found positive wiLh PCR and 30 samples were negative with PCR. 30 samples were smear positive and 30 samples were smear negative. 17 samples were culture positive and 30 samples were culture negative. The sensilivily and specificity were 94% and 79%. respectiv...
Diabetes mellitus is a leading cause of end-stage renal disease in the Western world. Histologica... more Diabetes mellitus is a leading cause of end-stage renal disease in the Western world. Histologically, mesangial expansion with increased extracellular matrix protein is observed in patients with diabetic nephropathy. Because transforming growth factor (TGF)-β promotes extracellular matrix production in response to high glucose, TGF-β is considered to play a central role in the pathogenesis of diabetic nephropathy. We investigated the
This study examines the frequency of TNFRSF1A gene R92Q mutation in patients with Familial Medite... more This study examines the frequency of TNFRSF1A gene R92Q mutation in patients with Familial Mediterranean Fever (FMF) and the role of this mutation in FMF. The study included 223 FMF patients with definite diagnosis, according to Tel-Hashomer criteria, carrying two mutations and 205 FMF patients as controls (symptomatically diagnosed) with definite diagnosis but without any of the MEFV gene mutations screened. The DNA samples of FMF patients and controls were genotyped with regard to TNFRSF1A gene R92Q mutation by PCR–RFLP method. Genotypes and allele frequencies of the TNFRSF1A gene R92Q mutation were similar in the two groups (p=0.481 and p=0.48, respectively). Because of the similarities between the symptoms of FMF and TNFRSF1A-associated periodic syndrome (TRAPS), the frequencies of the TNFRSF1A gene R92Q mutation was studied in patients with two MEFV gene mutations and also in patients without any of the twelve most common MEFV gene mutations. No significant difference was obser...
It has become increasingly clear in recent years that periodontal disease can cause dramatic incr... more It has become increasingly clear in recent years that periodontal disease can cause dramatic increases in the levels of markers of systemic inflam - mation and can also result in reductions in the levels of these markers. It is also known that amyloid fibril deposits derived from circulating acute-phase reactant serum amyloid A (SAA) lead to systemic AA amyloidosis. However, in up to approx- imately 12% of cases of AA amyloidosis no underlying etiology can be identified. Periodontal evaluations are not normally performed as a part of the medical as- sessment of patients with AA amyloidosis. Hence, destructive periodontal diseases may be an overlooked source of inflam - mation in these patients. Periodontal diseases have not been investigated in systemic AA amyloidosis with unknown etiology. Our hypothesis is that periodon- titis may be an important occult source of chronic inflammation that increases the levels of acute-phase reactants, which, in turn, might affect or cause the develop- ment of systemic AA amyloidosis. INTRODUCTION Periodontal diseases are moving into the focus of systemic diseases. Periodontitis is a chronic and occult infection. Our hypothesis is that periodontitis should be considered as a possible etiological factor along with the traditional factors for systemic AA
Background & objective: Genetic polymorphisms in the vitamin D receptor (VDR) gene are related to... more Background & objective: Genetic polymorphisms in the vitamin D receptor (VDR) gene are related to bone mineral density, bone turnover, and diseases with bone loss. Alveolar bone loss is a key feature in periodontitis. The aim of this study was to determine whether severe generalized chronic periodontitis (CP) in a Turkish population was associated with polymorphisms in the VDR gene. Methods: Samples of venous blood and DNA were obtained from 72 patients with severe generalized chronic periodontitis and 102 healthy controls. The polymorphic regions were amplified using PCR followed by digestion with restriction enzymes BsmI A/G(rs1544410), ApaI G/T(rs11168271), TaqI T/C(rs731236), and analyzed electrophoretically. Genotype and allele frequencies were calculated. Results: There were no statistically significant differences in the frequencies of VDR BsmI, ApaI, TaqI genotypes between the CP patients and healthy controls. The GTT haplotype, constructed from the three adjacent restriction fragment length polymorphisms was found to be over-represented among CP cases. This corresponded an OR of 2.4 (95% confidence interval, 1.12-5.18) for heterozygous carriers and 2.27 (95% confidence interval, 0.95-5.4) for homozygous carrier of the risk haplotype. Interpretation & conclusion: The present findings indicated that BsmI, ApaI, TaqI polymorphisms of the VDR gene were not associated with the severe generalized CP in the studied Turkish patients. Moreover, the VDR genotypes based on haplotype analysis may be associated with chronic periodontitis. In the future, diagnostic periodontal risk assessments like polymorphisms may be useful in detection of individuals susceptible for periodontitis.
Comparison of The Myobacterium Tuberculosis with The Conventional Methods Polymerase chain reacti... more Comparison of The Myobacterium Tuberculosis with The Conventional Methods Polymerase chain reaction (PCR). due to its faster response than culture was used for the diagnosis of Mycobacterium tuberculosis. Amplification reactions were performed using primers that amplify a 123 bp fragment of Insertion Sequence 6110 (IS61 10) specific to M. tuberculosis. A total of 64 samples were tested by PCR. The type (number) of specimens tested as fallows: sputum (31). urine (20). perisentez (5), torosentez (4). peritoneal fluid (3) and broneial washing (1). Sputum samples were decontaminated by N-acetyl-L-cysteine-NaOH. other samples were decontaminated by NaOH-Na citrate method. The DNA used in PCR was obtained by boiling. 33 samples were found positive wiLh PCR and 30 samples were negative with PCR. 30 samples were smear positive and 30 samples were smear negative. 17 samples were culture positive and 30 samples were culture negative. The sensilivily and specificity were 94% and 79%. respectiv...
Diabetes mellitus is a leading cause of end-stage renal disease in the Western world. Histologica... more Diabetes mellitus is a leading cause of end-stage renal disease in the Western world. Histologically, mesangial expansion with increased extracellular matrix protein is observed in patients with diabetic nephropathy. Because transforming growth factor (TGF)-β promotes extracellular matrix production in response to high glucose, TGF-β is considered to play a central role in the pathogenesis of diabetic nephropathy. We investigated the
This study examines the frequency of TNFRSF1A gene R92Q mutation in patients with Familial Medite... more This study examines the frequency of TNFRSF1A gene R92Q mutation in patients with Familial Mediterranean Fever (FMF) and the role of this mutation in FMF. The study included 223 FMF patients with definite diagnosis, according to Tel-Hashomer criteria, carrying two mutations and 205 FMF patients as controls (symptomatically diagnosed) with definite diagnosis but without any of the MEFV gene mutations screened. The DNA samples of FMF patients and controls were genotyped with regard to TNFRSF1A gene R92Q mutation by PCR–RFLP method. Genotypes and allele frequencies of the TNFRSF1A gene R92Q mutation were similar in the two groups (p=0.481 and p=0.48, respectively). Because of the similarities between the symptoms of FMF and TNFRSF1A-associated periodic syndrome (TRAPS), the frequencies of the TNFRSF1A gene R92Q mutation was studied in patients with two MEFV gene mutations and also in patients without any of the twelve most common MEFV gene mutations. No significant difference was obser...
It has become increasingly clear in recent years that periodontal disease can cause dramatic incr... more It has become increasingly clear in recent years that periodontal disease can cause dramatic increases in the levels of markers of systemic inflam - mation and can also result in reductions in the levels of these markers. It is also known that amyloid fibril deposits derived from circulating acute-phase reactant serum amyloid A (SAA) lead to systemic AA amyloidosis. However, in up to approx- imately 12% of cases of AA amyloidosis no underlying etiology can be identified. Periodontal evaluations are not normally performed as a part of the medical as- sessment of patients with AA amyloidosis. Hence, destructive periodontal diseases may be an overlooked source of inflam - mation in these patients. Periodontal diseases have not been investigated in systemic AA amyloidosis with unknown etiology. Our hypothesis is that periodon- titis may be an important occult source of chronic inflammation that increases the levels of acute-phase reactants, which, in turn, might affect or cause the develop- ment of systemic AA amyloidosis. INTRODUCTION Periodontal diseases are moving into the focus of systemic diseases. Periodontitis is a chronic and occult infection. Our hypothesis is that periodontitis should be considered as a possible etiological factor along with the traditional factors for systemic AA
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