Figure 4. Comparison of CCPs labeled with various probes.
Composite CCPs constructed by aligning the 3D center-of-mass of CCPs in an entire field of view for each probe. A portion of the field of view is displayed in Fig. 2b for Alexa647 and in Supplementary Fig. 8 for tdEos, mEos2, Atto655, TMR and Oregon Green. The xy cross-sections through the 3D center of the composite CCPs are displayed in the upper panels. The localization density profiles along the x axis within a 75-nm wide stripe at the center of the pits are shown in the lower panels. Scale bar, 100 nm.