Abstract
Previously, CD47 gene expression has been shown to increase during mandible development using a micro array technique. To determine the function of CD47 in osteoblasts, CD47 was silenced using siRNA in vitro. The TGF-β1 and phosphorylated-Smad2 levels and transcription factor genes related to bone metabolism increased dose-dependently with CD47 silencing. Furthermore, we determined the distribution of CD47 in mouse embryonic E13 and E15 in vivo. The CD47-positive cells were localized in Meckel's cartilage and antenatal mandibular bone. These results suggest that TGF-β1 signaling and mandible development might be regulated by CD47.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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3T3 Cells
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Animals
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Blotting, Western
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CD47 Antigen / analysis
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CD47 Antigen / genetics
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CD47 Antigen / physiology*
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Cartilage / embryology*
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Cell Culture Techniques
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Electrophoresis, Polyacrylamide Gel
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Gene Silencing / physiology
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Gestational Age
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Immunohistochemistry
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Mandible / embryology*
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Mandible / metabolism
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Mesoderm / physiology*
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Mice
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Osteoblasts / physiology*
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Polymerase Chain Reaction
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RNA, Small Interfering / genetics
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Signal Transduction / genetics
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Signal Transduction / physiology*
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Smad2 Protein / analysis
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Smad2 Protein / genetics
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Transcription Factors / genetics
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Transforming Growth Factor beta1 / analysis
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Transforming Growth Factor beta1 / genetics
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Transforming Growth Factor beta1 / physiology*
Substances
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CD47 Antigen
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Cd47 protein, mouse
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RNA, Small Interfering
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Smad2 Protein
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Smad2 protein, mouse
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Transcription Factors
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Transforming Growth Factor beta1