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Circadian clock mutants in Arabidopsis identified by luciferase imaging

Science. 1995 Feb 24;267(5201):1161-3. doi: 10.1126/science.7855595.

Abstract

The cycling bioluminescence of Arabidopsis plants carrying a firefly luciferase fusion construct was used to identify mutant individuals with aberrant cycling patterns. Both long- and short-period mutants were recovered. A semidominant short-period mutation, timing of CAB expression (toc1), was mapped to chromosome 5. The toc1 mutation shortens the period of two distinct circadian rhythms, the expression of chlorophyll a/b-binding protein (CAB) genes and the movements of primary leaves, although toc1 mutants do not show extensive pleiotropy for other phenotypes.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Arabidopsis / genetics
  • Arabidopsis / physiology*
  • Arabidopsis Proteins*
  • Biological Clocks / genetics*
  • Carrier Proteins / genetics*
  • Circadian Rhythm / genetics*
  • Crosses, Genetic
  • Darkness
  • Gene Expression Regulation, Plant
  • Genes, Plant*
  • Light
  • Light-Harvesting Protein Complexes
  • Luciferases / genetics
  • Luminescence
  • Movement
  • Mutation
  • Phenotype
  • Photosynthetic Reaction Center Complex Proteins*
  • Photosystem II Protein Complex*
  • Plant Leaves / physiology
  • Plant Proteins*
  • Plants, Genetically Modified
  • Recombinant Fusion Proteins

Substances

  • Arabidopsis Proteins
  • CAB1 protein, Arabidopsis
  • Carrier Proteins
  • Light-Harvesting Protein Complexes
  • Photosynthetic Reaction Center Complex Proteins
  • Photosystem II Protein Complex
  • Plant Proteins
  • Recombinant Fusion Proteins
  • Luciferases