This article presents the LIFEisGAME project, a serious game that will help children with ASDs to... more This article presents the LIFEisGAME project, a serious game that will help children with ASDs to recognize and express emotions through facial expressions. The game design tackles the main experiential learning cycle of emotion recognition: watch and recognize, learn by doing, recognize and mimic, generalize or knowledge transfer to real life. We briefly describe the technology behind the character animation pipeline centered on the creation of a generic rig. Then, we detail the facial expression analyzer that uses Active Appearance Models. Last, we describe the user study experiment using game mode “recognize the expression”.
This article presents the LIFEisGAME project, a serious game that will help children with ASDs to... more This article presents the LIFEisGAME project, a serious game that will help children with ASDs to recognize and express emotions through facial expressions. The game design tackles the main experiential learning cycle of emotion recognition: watch and recognize, learn by doing, recognize and mimic, generalize or knowledge transfer to real life. We briefly describe the technology behind the character animation pipeline centered on the creation of a generic rig. Then, we detail the facial expression analyzer that uses Active Appearance Models. Last, we describe the user study experiment using game mode “recognize the expression”.
There is a need for a deeper understanding of the biochemical events affecting embryonic stem (ES... more There is a need for a deeper understanding of the biochemical events affecting embryonic stem (ES) cell culture by analyzing the expansion of mouse ES cells in terms of both cell growth and metabolic kinetics. The influence of the initial cell density on cell expansion was assessed. Concomitantly, the biochemical profile of the culture was evaluated, which allowed measuring the consumption of important substrates, such as glucose and glutamine, and the production of metabolic byproducts, like lactate. The results suggest a more efficient cell metabolism in serum-free conditions and a preferential use of glutaminolysis as an energy source during cell expansion at low seeding densities. This work contributes to the development of fully-controlled bioprocesses to produce relevant numbers of ES cells for cell therapies and high-throughput drug screening.
We have studied the effect of low oxygen levels (2% O2), or hypoxia, in the expansion and neural ... more We have studied the effect of low oxygen levels (2% O2), or hypoxia, in the expansion and neural commitment of mouse embryonic stem (ES) cells. When ES cells were maintained in culture with leukemia inhibitory factor (LIF), cell proliferation was reduced at low oxygen levels and a simultaneous reduction in cell viability was also observed. Morphological changes and different cell cycle patterns also occurred, suggesting some early differentiation under hypoxic conditions. However, when cells were maintained in a ground state of pluripotency, by inhibition of autocrine FGF4/ERK and GSK3 signaling, hypoxia did not affect cell proliferation, and did not induce early differentiation. Nevertheless, during neural commitment, low oxygen tension exerted a positive effect on early differentiation of ES cells, resulting in a faster commitment towards neural progenitors. Overall our results demonstrate the need to specifically regulate the oxygen content, especially hypoxia, along with other culture conditions, when developing new strategies for ES cell expansion and/or controlled differentiation.
This article presents the LIFEisGAME project, a serious game that will help children with ASDs to... more This article presents the LIFEisGAME project, a serious game that will help children with ASDs to recognize and express emotions through facial expressions. The game design tackles the main experiential learning cycle of emotion recognition: watch and recognize, learn by doing, recognize and mimic, generalize or knowledge transfer to real life. We briefly describe the technology behind the character animation pipeline centered on the creation of a generic rig. Then, we detail the facial expression analyzer that uses Active Appearance Models. Last, we describe the user study experiment using game mode “recognize the expression”.
This article presents the LIFEisGAME project, a serious game that will help children with ASDs to... more This article presents the LIFEisGAME project, a serious game that will help children with ASDs to recognize and express emotions through facial expressions. The game design tackles the main experiential learning cycle of emotion recognition: watch and recognize, learn by doing, recognize and mimic, generalize or knowledge transfer to real life. We briefly describe the technology behind the character animation pipeline centered on the creation of a generic rig. Then, we detail the facial expression analyzer that uses Active Appearance Models. Last, we describe the user study experiment using game mode “recognize the expression”.
There is a need for a deeper understanding of the biochemical events affecting embryonic stem (ES... more There is a need for a deeper understanding of the biochemical events affecting embryonic stem (ES) cell culture by analyzing the expansion of mouse ES cells in terms of both cell growth and metabolic kinetics. The influence of the initial cell density on cell expansion was assessed. Concomitantly, the biochemical profile of the culture was evaluated, which allowed measuring the consumption of important substrates, such as glucose and glutamine, and the production of metabolic byproducts, like lactate. The results suggest a more efficient cell metabolism in serum-free conditions and a preferential use of glutaminolysis as an energy source during cell expansion at low seeding densities. This work contributes to the development of fully-controlled bioprocesses to produce relevant numbers of ES cells for cell therapies and high-throughput drug screening.
We have studied the effect of low oxygen levels (2% O2), or hypoxia, in the expansion and neural ... more We have studied the effect of low oxygen levels (2% O2), or hypoxia, in the expansion and neural commitment of mouse embryonic stem (ES) cells. When ES cells were maintained in culture with leukemia inhibitory factor (LIF), cell proliferation was reduced at low oxygen levels and a simultaneous reduction in cell viability was also observed. Morphological changes and different cell cycle patterns also occurred, suggesting some early differentiation under hypoxic conditions. However, when cells were maintained in a ground state of pluripotency, by inhibition of autocrine FGF4/ERK and GSK3 signaling, hypoxia did not affect cell proliferation, and did not induce early differentiation. Nevertheless, during neural commitment, low oxygen tension exerted a positive effect on early differentiation of ES cells, resulting in a faster commitment towards neural progenitors. Overall our results demonstrate the need to specifically regulate the oxygen content, especially hypoxia, along with other culture conditions, when developing new strategies for ES cell expansion and/or controlled differentiation.
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