I am a PhD candidate in 3D cell culture for T cell development and cancer immunology at Pusan national university. i am working in the Immune cell molecular biology Research Lab (http://medical.pusan.ac.kr, http://cafe.daum.net/immunohistocell), under the supervision of Professor Dr. Yoon Sik. i earned my Pharmacy degree from the University of Malakand, Pakistan in 2009 and completed clinical clerkship in the Hayatabad medical complex Hospital and Medicraft pharmaceutical, both in Peshawar, Pakistan. after that had also work experience as dispensing pharmacist with an international NGO "International medical Corps (IMC)" to provide free medical care to patients in the flood and earthquake affected areas in Swat Pakistan. Latter received my master’s degree in medicine from the Pusan national university, Busan, South Korea. My master thesis versed on “Modulation of the adhesion of thymic epithelial cells with thymocyte by gain- and loss-of-function of mouse epidermal growth factor-like protein 8”. Currently my work focuses on the use of 3D cell culture to study the T cell development and designing the matrix for normal and cancerous tissue, organoids and tumaroids development particularly to the find out the hematological malignancy and metastasis of lymphoma. My work also addresses cellular and molecular mechanistic role of anticancer drugs resistance in cancer malignancy (Lymphoma) and their sensitization. I was one of the selected candidates in Korea in 2011 - till date to be awarded with scholarships for the role of epidermal growth factor like protein 8, BK-21 plus programs and immune network pioneer research center, which provides full tuition and stipend support to students to pursue Graduate studies in South Korea. Address: Pusan, Pusan-jikhalsi, Korea, Republic of
Hydrogels are prototypical matrices for 3D cell culture, of which alginate hydrogels are extensiv... more Hydrogels are prototypical matrices for 3D cell culture, of which alginate hydrogels are extensively used. However, ionic crosslinking agents, such as Ca 2+ , are required to form alginate hydrogels, but introduce Ca 2+-associated cytotoxicity and long-term stability issues. Collagen is a promising biomaterial for 3D cell culture scaffolds primarily due to its biocompatibility. In the present study, the authors designed and fabricated a calcium-free, physically crosslinked, efficient, and bioactive hydrogel composed of alginate, marine collagen, and agarose (AmCA) for use in 3D cell cultures. This AmCA hydrogel was assessed by FTIR, swelling property, scanning electron microscopy, phase contrast microscopy, cell proliferation, cell viability, confocal microscopy, transparency and RT-PCR analyses. The gel was found to exhibit excellent cytocompatibility with various tumor and non-tumor cells, to generate high yields of multicellular spheroids, and to promote cellular activity. Furthermore, the transparency of the AmCA hydrogel suggests it can be used without cell-tracking chemicals in morphological studies of cell cultures. Taken together, it would appear that the described physically crosslinked AmCA hydrogel could provide a novel platform for the development of customizable, transparent, biocompatible, functional, easy-to-produce, and cost-effective scaffolds for use in 3D cultures of various cell types.
Single pH-dependent drug delivery systems have been widely used for colon-targeted delivery, but ... more Single pH-dependent drug delivery systems have been widely used for colon-targeted delivery, but their efficiency is often hampered by the variation in gut pH. To overcome the limitation of single pH-dependent delivery systems, in this study, we developed and evaluated the therapeutic potential of budesonide-loaded dual pH/time-dependent nanoparticles (NPs) for the treatment of colitis. Eudragit FS30D was used as a pH-dependent polymer, and Eudragit RS100 as a time-dependent controlled release polymer. Single pH-dependent NPs (pH_NPs), single time-dependent NPs (Time_NPs), and dual pH/time-dependent NPs (pH/Time_NPs) were prepared using the oil-in-water emulsion method. The physicochemical properties and drug release profiles of these NPs in gastrointestinal (GI) tract conditions were investigated. The therapeutic potential and in vivo distribution of the NPs were evaluated in a dextran sulfate sodium (DSS)-induced colitis mice model. The pH/Time_NPs prevented a burst drug release in acidic pH conditions and showed sustained release at a colonic pH. The in vivo distribution study in the mice GI tract demonstrated that pH/Time_NPs were more efficiently delivered to the inflamed colon than pH_NPs were. Compared to the single pH_NPs-treated group, the pH/Time_NPs-treated group showed increased body weight and colon length and markedly decreased disease activity index, colon weight/length ratios, histological damage, and inflammatory cell infiltration in colon tissue. Our results demonstrate that the dual pH/time-dependent NPs are an effective oral colon-targeted delivery system for colitis therapy.
In this study, we sought to synthesize regenerated bacterial cellulose (rBC) scaffolds for applic... more In this study, we sought to synthesize regenerated bacterial cellulose (rBC) scaffolds for application in in vitro tissue regeneration. Bacterial cellulose (BC) was dissolved in N-methyl morpholine-N-oxide (NMMO), and salt crystals were added as porogens, followed by casting in molds and incubation in water. The synthesized scaffolds were characterized using Fourier transform infrared (FT-IR) spectroscopy and field-emission scanning electron microscopy (FE-SEM). The FT-IR spectra exhibited bands characteristic for BC in rBC scaffolds, indicating no alteration in chemical structure, while FE-SEM revealed a porous structure of the rBC scaffold. The scaffolds exhibited very high swelling ratio, indicating enhanced water absorption and nutrient exchange capacity. The in vitro biocompatibility of the rBC scaffolds was tested based on the adhesion, growth, and proliferation of animal fibroblasts (NIH 3T3), and the osteogenesis of animal osteoblasts (MC3T3-E1). Results indicated good cell adhesion, penetration, and proliferation. Alkaline phosphatase (ALP) activity and Alizarin red staining (ARS) revealed osteogenic differentiation of animal osteoblasts on the scaffolds. These results demonstrate that the rBC scaffolds are potential candidates for future tissue engineering applications.
Hydrogels are prototypical matrices for 3D cell culture, of which alginate hydrogels are extensiv... more Hydrogels are prototypical matrices for 3D cell culture, of which alginate hydrogels are extensively used. However, ionic crosslinking agents, such as Ca 2+ , are required to form alginate hydrogels, but introduce Ca 2+-associated cytotoxicity and long-term stability issues. Collagen is a promising biomaterial for 3D cell culture scaffolds primarily due to its biocompatibility. In the present study, the authors designed and fabricated a calcium-free, physically crosslinked, efficient, and bioactive hydrogel composed of alginate, marine collagen, and agarose (AmCA) for use in 3D cell cultures. This AmCA hydrogel was assessed by FTIR, swelling property, scanning electron microscopy, phase contrast microscopy, cell proliferation, cell viability, confocal microscopy, transparency and RT-PCR analyses. The gel was found to exhibit excellent cytocompatibility with various tumor and non-tumor cells, to generate high yields of multicellular spheroids, and to promote cellular activity. Furthermore, the transparency of the AmCA hydrogel suggests it can be used without cell-tracking chemicals in morphological studies of cell cultures. Taken together, it would appear that the described physically crosslinked AmCA hydrogel could provide a novel platform for the development of customizable, transparent, biocompatible, functional, easy-to-produce, and cost-effective scaffolds for use in 3D cultures of various cell types.
Single pH-dependent drug delivery systems have been widely used for colon-targeted delivery, but ... more Single pH-dependent drug delivery systems have been widely used for colon-targeted delivery, but their efficiency is often hampered by the variation in gut pH. To overcome the limitation of single pH-dependent delivery systems, in this study, we developed and evaluated the therapeutic potential of budesonide-loaded dual pH/time-dependent nanoparticles (NPs) for the treatment of colitis. Eudragit FS30D was used as a pH-dependent polymer, and Eudragit RS100 as a time-dependent controlled release polymer. Single pH-dependent NPs (pH_NPs), single time-dependent NPs (Time_NPs), and dual pH/time-dependent NPs (pH/Time_NPs) were prepared using the oil-in-water emulsion method. The physicochemical properties and drug release profiles of these NPs in gastrointestinal (GI) tract conditions were investigated. The therapeutic potential and in vivo distribution of the NPs were evaluated in a dextran sulfate sodium (DSS)-induced colitis mice model. The pH/Time_NPs prevented a burst drug release in acidic pH conditions and showed sustained release at a colonic pH. The in vivo distribution study in the mice GI tract demonstrated that pH/Time_NPs were more efficiently delivered to the inflamed colon than pH_NPs were. Compared to the single pH_NPs-treated group, the pH/Time_NPs-treated group showed increased body weight and colon length and markedly decreased disease activity index, colon weight/length ratios, histological damage, and inflammatory cell infiltration in colon tissue. Our results demonstrate that the dual pH/time-dependent NPs are an effective oral colon-targeted delivery system for colitis therapy.
In this study, we sought to synthesize regenerated bacterial cellulose (rBC) scaffolds for applic... more In this study, we sought to synthesize regenerated bacterial cellulose (rBC) scaffolds for application in in vitro tissue regeneration. Bacterial cellulose (BC) was dissolved in N-methyl morpholine-N-oxide (NMMO), and salt crystals were added as porogens, followed by casting in molds and incubation in water. The synthesized scaffolds were characterized using Fourier transform infrared (FT-IR) spectroscopy and field-emission scanning electron microscopy (FE-SEM). The FT-IR spectra exhibited bands characteristic for BC in rBC scaffolds, indicating no alteration in chemical structure, while FE-SEM revealed a porous structure of the rBC scaffold. The scaffolds exhibited very high swelling ratio, indicating enhanced water absorption and nutrient exchange capacity. The in vitro biocompatibility of the rBC scaffolds was tested based on the adhesion, growth, and proliferation of animal fibroblasts (NIH 3T3), and the osteogenesis of animal osteoblasts (MC3T3-E1). Results indicated good cell adhesion, penetration, and proliferation. Alkaline phosphatase (ALP) activity and Alizarin red staining (ARS) revealed osteogenic differentiation of animal osteoblasts on the scaffolds. These results demonstrate that the rBC scaffolds are potential candidates for future tissue engineering applications.
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in vitro tissue regeneration. Bacterial cellulose (BC) was dissolved in N-methyl morpholine-N-oxide
(NMMO), and salt crystals were added as porogens, followed by casting in molds and incubation in
water. The synthesized scaffolds were characterized using Fourier transform infrared (FT-IR)
spectroscopy and field-emission scanning electron microscopy (FE-SEM). The FT-IR spectra exhibited
bands characteristic for BC in rBC scaffolds, indicating no alteration in chemical structure, while FE-SEM
revealed a porous structure of the rBC scaffold. The scaffolds exhibited very high swelling ratio,
indicating enhanced water absorption and nutrient exchange capacity. The in vitro biocompatibility of
the rBC scaffolds was tested based on the adhesion, growth, and proliferation of animal fibroblasts (NIH
3T3), and the osteogenesis of animal osteoblasts (MC3T3-E1). Results indicated good cell adhesion,
penetration, and proliferation. Alkaline phosphatase (ALP) activity and Alizarin red staining (ARS) revealed
osteogenic differentiation of animal osteoblasts on the scaffolds. These results demonstrate that the rBC
scaffolds are potential candidates for future tissue engineering applications.
in vitro tissue regeneration. Bacterial cellulose (BC) was dissolved in N-methyl morpholine-N-oxide
(NMMO), and salt crystals were added as porogens, followed by casting in molds and incubation in
water. The synthesized scaffolds were characterized using Fourier transform infrared (FT-IR)
spectroscopy and field-emission scanning electron microscopy (FE-SEM). The FT-IR spectra exhibited
bands characteristic for BC in rBC scaffolds, indicating no alteration in chemical structure, while FE-SEM
revealed a porous structure of the rBC scaffold. The scaffolds exhibited very high swelling ratio,
indicating enhanced water absorption and nutrient exchange capacity. The in vitro biocompatibility of
the rBC scaffolds was tested based on the adhesion, growth, and proliferation of animal fibroblasts (NIH
3T3), and the osteogenesis of animal osteoblasts (MC3T3-E1). Results indicated good cell adhesion,
penetration, and proliferation. Alkaline phosphatase (ALP) activity and Alizarin red staining (ARS) revealed
osteogenic differentiation of animal osteoblasts on the scaffolds. These results demonstrate that the rBC
scaffolds are potential candidates for future tissue engineering applications.